Restriction map for
pFN21AB8108
(Circular) (Six-base) MAPSORT of: pFN21AB8108.seq Check: 5749 from: 1 to: 6139
pFN21AB8108 6139 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3320 4202 5586 5854 1632
Size: 1688 882 1384 268 1917
Fragments arranged by size:
1917 1688 1384 882 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 3175 3404 5038 6015 1203
Size: 1972 229 1634 977 1327
Fragments arranged by size:
1972 1634 1327 977 229
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 2871 2903 3372 3404 2319
Size: 32 520 32 469 32 5054
Fragments arranged by size:
5054 520 469 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3882 5454 3882
Size: 1572 4567
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3160 3448 3674 3826 5935 1493
Size: 1667 288 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 288 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 3464 1870
Size: 73 1521 4545
Fragments arranged by size:
4545 1521 73
BamHI G'GATC_C
Cuts at: 2059 3469 3836 2059
Size: 1410 367 4362
Fragments arranged by size:
4362 1410 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3460 4915 618
Size: 325 217 627 1673 1455 1842
Fragments arranged by size:
1842 1673 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3458 1391
Size: 2067 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 2963 928
Size: 971 714 350 4104
Fragments arranged by size:
4104 971 714 350
BbvCI CC'TCA_GC
Cuts at: 2097 2097
Size: 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2385 4031 5241 2385
Size: 1646 1210 3283
Fragments arranged by size:
3283 1646 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 3187 4750
Size: 107 122 71 631 605 421 1094 1563
Cuts at: 4750 136
Size: 1525
Fragments arranged by size:
1563 1525 1094 631 605 421 122 107
71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 4200 5356 1790
Size: 2410 1156 2573
Fragments arranged by size:
2573 2410 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3870 4828 442
Size: 416 53 83 399 24 2453 958 1753
Fragments arranged by size:
2453 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4799 1239
Size: 3560 2579
Bpu10I CC'TnA_GC
Cuts at: 2097 2097
Size: 6139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4802 882
Size: 617 3303 2219
Fragments arranged by size:
3303 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2551 3930 493
Size: 711 1347 1379 2702
Fragments arranged by size:
2702 1379 1347 711
BsaBI GATnn'nnATC
Cuts at: 2588 3333 6133 2588
Size: 745 2800 2594
Fragments arranged by size:
2800 2594 745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2178 2208 2332 2362 1178
Size: 30 165 30 775 30 124 30 4955
Fragments arranged by size:
4955 775 165 124 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 3441 1186
Size: 847 1408 3884
Fragments arranged by size:
3884 1408 847
BseYI C'CCAG_C
Cuts at: 5342 5838 5342
Size: 496 5643
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4200 4285 5356 4200
Size: 85 1071 4983
Fragments arranged by size:
4983 1071 85
BsmI GAATG_Cn'
Cuts at: 2411 3650 3743 2411
Size: 1239 93 4807
Fragments arranged by size:
4807 1239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3458 4200 4285 5356 1391
Size: 399 1668 742 85 1071 2174
Fragments arranged by size:
2174 1668 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 3127 4028 5758 3127
Size: 901 1730 3508
Fragments arranged by size:
3508 1730 901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3494 844
Size: 2650 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4026 1394
Size: 429 2203 3507
Fragments arranged by size:
3507 2203 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2711 4783 4970 1700
Size: 110 901 2072 187 2869
Fragments arranged by size:
2869 2072 901 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2048 2048
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 2059 3328 3469 3836 4221 4238 5690 5776
Size: 87 1269 141 367 385 17 1452 86
Cuts at: 5776 5788 6134 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1269 385 367 346 141 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4648
Fragments arranged by size:
4648 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2289 487
Size: 683 56 1063 4337
Fragments arranged by size:
4337 1063 683 56
BtsI GCAGTG_nn'
Cuts at: 3655 4449 4469 3655
Size: 794 20 5325
Fragments arranged by size:
5325 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3586 4475 6104
Size: 54 1425 50 275 18 1756 889 1629
Cuts at: 6104 8
Size: 43
Fragments arranged by size:
1756 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2315 2487 4069 1353
Size: 776 186 172 1582 3423
Fragments arranged by size:
3423 1582 776 186 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3320 4202 4799 5586 5854 1239
Size: 393 1688 882 597 787 268 1524
Fragments arranged by size:
1688 1524 882 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2144 2144
Size: 6139
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 5286 1678
Size: 3608 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2336 2368 2661
Size: 32 135 32 593 32 139 32 293
Cuts at: 2661 2693 4780 4812 4854 4886 1373
Size: 32 2087 32 42 32 2626
Fragments arranged by size:
2626 2087 593 293 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 3000 3090 3738 3000
Size: 90 648 5401
Fragments arranged by size:
5401 648 90
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 2583 3151 3425 5253 5437 1305
Size: 891 387 568 274 1828 184 2007
Fragments arranged by size:
2007 1828 891 568 387 274 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4317
Fragments arranged by size:
4317 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2046 2772 3343 4097 4456 4615 518
Size: 648 880 726 571 754 359 159 2042
Fragments arranged by size:
2042 880 754 726 648 571 359 159
MspA1I CmG'CkG
Cuts at: 1606 2441 3555 4232 5380 5625 1606
Size: 835 1114 677 1148 245 2120
Fragments arranged by size:
2120 1148 1114 835 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4648
Fragments arranged by size:
4648 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 3023 3179 3408 3497 5042 1132
Size: 97 1794 156 229 89 1545 2229
Fragments arranged by size:
2229 1794 1545 229 156 97 89
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 3175 3404 5038 3175
Size: 229 1634 4276
Fragments arranged by size:
4276 1634 229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6139
PfoI T'CCnGG_A
Cuts at: 2033 2033
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PstI C_TGCA'G
Cuts at: 830 2155 2524 3491 830
Size: 1325 369 967 3478
Fragments arranged by size:
3478 1325 967 369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2441 2441
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SapI GCTCTTCn'nnn_
Cuts at: 2129 2129
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SfcI C'TryA_G
Cuts at: 826 1045 2151 2520 3487 4622 5303 5494 826
Size: 219 1106 369 967 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 967 681 369 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 5144 5406 820
Size: 197 926 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2551 3930 493
Size: 2058 1379 2702
Fragments arranged by size:
2702 2058 1379
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 2652 1517
Size: 1135 5004
StyI C'CwwG_G
Cuts at: 513 1065 2004 3578 513
Size: 552 939 1574 3074
Fragments arranged by size:
3074 1574 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2434 4166 4183 4336 4521 4966 325
Size: 1255 854 1732 17 153 185 445 1498
Fragments arranged by size:
1732 1498 1255 854 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3107 4385 6097
Size: 274 80 33 51 1443 1130 1278 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
1712 1443 1278 1130 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 2797 2829 3018 3050 6099 6131 2178
Size: 32 587 32 189 32 3049 32 2186
Fragments arranged by size:
3049 2186 587 189 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI