Restriction map for
pFN21AB8102
(Circular) (Six-base) MAPSORT of: pFN21AB8102.seq Check: 9166 from: 1 to: 5956
pFN21AB8102 5956 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2539 4019 5403 5671 1632
Size: 907 1480 1384 268 1917
Fragments arranged by size:
1917 1480 1384 907 268
AfeI AGC'GCT
Cuts at: 2985 2985
Size: 5956
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 2330 4855 5832 1203
Size: 1127 2525 977 1327
Fragments arranged by size:
2525 1327 1127 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 2222 2756 3036 1166
Size: 1056 534 280 4086
Fragments arranged by size:
4086 1056 534 280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2732 1184
Size: 1548 4408
AlwNI CAG_nnn'CTG
Cuts at: 2241 3699 5271 2241
Size: 1458 1572 2926
Fragments arranged by size:
2926 1572 1458
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2477 2681 2813 2849 3173
Size: 325 217 627 690 204 132 36 324
Cuts at: 3173 3277 4732 618
Size: 104 1455 1842
Fragments arranged by size:
1842 1455 690 627 325 324 217 204
132 104 36
BanII G_rGCy'C
Cuts at: 1391 2402 2654 3275 1391
Size: 1011 252 621 4072
Fragments arranged by size:
4072 1011 621 252
BbeI G_GCGC'C
Cuts at: 2817 2817
Size: 5956
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BfrBI ATG'CAT
Cuts at: 2074 2074
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3111 4567
Size: 107 122 71 631 605 337 1102 1456
Cuts at: 4567 136
Size: 1525
Fragments arranged by size:
1525 1456 1102 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2646 2852 3178 4017 5173 1790
Size: 856 206 326 839 1156 2573
Fragments arranged by size:
2573 1156 856 839 326 206
BmgBI CAC'GTC
Cuts at: 1318 3034 1318
Size: 1716 4240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2262 3687 4645
Size: 416 53 83 399 24 845 1425 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1425 958 845 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2852 4616 1239
Size: 1613 1764 2579
Fragments arranged by size:
2579 1764 1613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2940 4619 882
Size: 617 1441 1679 2219
Fragments arranged by size:
2219 1679 1441 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 3158 5950 3158
Size: 2792 3164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2814 2940 3763 4145
Size: 53 83 186 936 1281 126 823 382
Cuts at: 4145 4780 275
Size: 635 1451
Fragments arranged by size:
1451 1281 936 823 635 382 186 126
83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 1178
Size: 30 165 30 819 30 4882
Fragments arranged by size:
4882 819 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3041 3137 3244 1186
Size: 1855 96 107 3898
Fragments arranged by size:
3898 1855 107 96
BseYI C'CCAG_C
Cuts at: 2621 5159 5655 2621
Size: 2538 496 2922
Fragments arranged by size:
2922 2538 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2822 1163
Size: 573 1086 4297
Fragments arranged by size:
4297 1086 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2402 3033 4017 4102 5173 2229
Size: 173 631 984 85 1071 3012
Fragments arranged by size:
3012 1071 984 631 173 85
BsmI GAATG_Cn'
Cuts at: 2887 3467 3560 2887
Size: 580 93 5283
Fragments arranged by size:
5283 580 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2402 2646 2654 2852 3033 3178
Size: 399 439 173 244 8 198 181 145
Cuts at: 3178 3275 4017 4102 5173 1391
Size: 97 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 244 198 181
173 145 97 85 8
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 2349 3845 5575 2349
Size: 1496 1730 2730
Fragments arranged by size:
2730 1730 1496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 2702 3311 844
Size: 1367 491 609 3489
Fragments arranged by size:
3489 1367 609 491
BsrBI CCG'CTC
Cuts at: 1394 1823 2679 3843 1394
Size: 429 856 1164 3507
Fragments arranged by size:
3507 1164 856 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2097 4451 4625 65
Size: 1183 849 2354 174 1396
Fragments arranged by size:
2354 1396 1183 849 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2895 4600 4787 1700
Size: 110 1085 1705 187 2869
Fragments arranged by size:
2869 1705 1085 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2273 2317 4016 5028 2273
Size: 44 1699 1012 3201
Fragments arranged by size:
3201 1699 1012 44
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 3166 3166
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951
Size: 1314 367 385 17 1452 86 12 346
Cuts at: 5951 1972
Size: 1977
Fragments arranged by size:
1977 1452 1314 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3145 3190 487
Size: 683 56 1919 45 3253
Fragments arranged by size:
3253 1919 683 56 45
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 2959 3260 2959
Size: 301 5655
DraIII CAC_nnn'GTG
Cuts at: 2576 2824 2576
Size: 248 5708
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2703 2796
Size: 54 1425 50 275 18 303 570 93
Cuts at: 2796 3103 3403 4292 5921 8
Size: 307 300 889 1629 43
Fragments arranged by size:
1629 1425 889 570 307 303 300 275
93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2284 2870 3221 3886 1353
Size: 931 586 351 665 3423
Fragments arranged by size:
3423 931 665 586 351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2400 2400
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2539 2852 4019 4616 5403 5671 1239
Size: 393 907 313 1167 597 787 268 1524
Fragments arranged by size:
1524 1167 907 787 597 393 313 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2737 2737
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2691 2691
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2025 2609 2817 2987 5103 1678
Size: 347 584 208 170 2116 2531
Fragments arranged by size:
2531 2116 584 347 208 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2286 2318 4597
Size: 32 135 32 620 32 62 32 2279
Cuts at: 4597 4629 4671 4703 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2279 620 135 62 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KasI G'GCGC_C
Cuts at: 2813 2813
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2568 5070 5254 1305
Size: 1263 2502 184 2007
Fragments arranged by size:
2502 2007 1263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2705 3105 10
Size: 54 1425 343 303 570 400 2861
Fragments arranged by size:
2861 1425 570 400 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2756 3036 3164 3914 4273 4432
Size: 648 1056 534 280 128 750 359 159
Cuts at: 4432 518
Size: 2042
Fragments arranged by size:
2042 1056 750 648 534 359 280 159
128
MspA1I CmG'CkG
Cuts at: 1606 2722 2802 3372 4049 5197 5442 1606
Size: 1116 80 570 677 1148 245 2120
Fragments arranged by size:
2120 1148 1116 677 570 245 80
NarI GG'CG_CC
Cuts at: 2814 2814
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NsiI A_TGCA'T
Cuts at: 2076 2076
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3151 3314 4859 1132
Size: 97 1922 163 1545 2229
Fragments arranged by size:
2229 1922 1545 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2691 2691
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 2268 1613
Size: 655 5301
PsiI TTA'TAA
Cuts at: 2364 3526 2364
Size: 1162 4794
PstI C_TGCA'G
Cuts at: 830 2721 2991 3308 830
Size: 1891 270 317 3478
Fragments arranged by size:
3478 1891 317 270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2722 2722
Size: 5956
SacI G_AGCT'C
Cuts at: 2402 2402
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SapI GCTCTTCn'nnn_
Cuts at: 2870 2870
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SexAI A'CCwGG_T
Cuts at: 2820 2820
Size: 5956
SfcI C'TryA_G
Cuts at: 826 1045 2717 2987 3304 4439 5120 5311 826
Size: 219 1672 270 317 1135 681 191 1471
Fragments arranged by size:
1672 1471 1135 681 317 270 219 191
SfoI GGC'GCC
Cuts at: 2815 2815
Size: 5956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3151 3314 1132
Size: 2019 163 3774
Fragments arranged by size:
3774 2019 163
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2590 1517
Size: 1073 4883
StyI C'CwwG_G
Cuts at: 513 1065 2004 3395 513
Size: 552 939 1391 3074
Fragments arranged by size:
3074 1391 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2495 2635 3158 3983 4000 4153 4338
Size: 1255 915 140 523 825 17 153 185
Cuts at: 4338 4783 325
Size: 445 1498
Fragments arranged by size:
1498 1255 915 825 523 445 185 153
140 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 4202 5914
Size: 274 80 33 51 1443 690 1535 1712
Cuts at: 5914 96
Size: 138
Fragments arranged by size:
1712 1535 1443 690 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2370 2402 5916 5948 2370
Size: 32 3514 32 2378
Fragments arranged by size:
3514 2378 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI
XcmI