Restriction map for
pFN21AB8101
(Circular) (Six-base) MAPSORT of: pFN21AB8101.seq Check: 4615 from: 1 to: 6046
pFN21AB8101 6046 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2517 3327 3856 278
Size: 53 83 186 936 981 810 529 2468
Fragments arranged by size:
2468 981 936 810 529 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2282 2334 2517 3389 1937
Size: 345 52 183 872 4594
Fragments arranged by size:
4594 872 345 183 52
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4109 5493 5761 1632
Size: 2477 1384 268 1917
Fragments arranged by size:
2477 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 3003 4945 5922 1203
Size: 1800 1942 977 1327
Fragments arranged by size:
1942 1800 1327 977
AgeI A'CCGG_T
Cuts at: 2039 4877 2039
Size: 2838 3208
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 3071 1166
Size: 1905 4141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2179 3789 5361 2179
Size: 1610 1572 2864
Fragments arranged by size:
2864 1610 1572
ApaI G_GGCC'C
Cuts at: 2190 2190
Size: 6046
ApaLI G'TGCA_C
Cuts at: 4103 5259 4103
Size: 1156 4890
ApoI r'AATT_y
Cuts at: 1493 2240 3355 3581 3733 5842 1493
Size: 747 1115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 747 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 3371 1870
Size: 73 1428 4545
Fragments arranged by size:
4545 1428 73
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3367 4822 618
Size: 325 217 627 1580 1455 1842
Fragments arranged by size:
1842 1580 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2190 2672 3129 3171 3365 1391
Size: 744 55 482 457 42 194 4072
Fragments arranged by size:
4072 744 482 457 194 55 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2921 3938 5148 2921
Size: 1017 1210 3819
Fragments arranged by size:
3819 1210 1017
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BfrBI ATG'CAT
Cuts at: 2055 2055
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2349 6041 2349
Size: 3692 2354
BlpI GC'TnA_GC
Cuts at: 3339 3463 3339
Size: 124 5922
Bme1580I G_kGCm'C
Cuts at: 1790 2190 4107 5263 1790
Size: 400 1917 1156 2573
Fragments arranged by size:
2573 1917 1156 400
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3777 4735 442
Size: 416 53 83 399 24 2360 958 1753
Fragments arranged by size:
2360 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2201 3134 4706 1239
Size: 932 30 933 1572 2579
Fragments arranged by size:
2579 1572 933 932 30
Bpu10I CC'TnA_GC
Cuts at: 2915 2915
Size: 6046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 4171 5036 5334 2633
Size: 1538 865 298 3345
Fragments arranged by size:
3345 1538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 2609 3071 4709 882
Size: 617 549 561 462 1638 2219
Fragments arranged by size:
2219 1638 617 561 549 462
BsaAI yAC'GTr
Cuts at: 493 1204 3004 3837 493
Size: 711 1800 833 2702
Fragments arranged by size:
2702 1800 833 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2514 2943 3324 3853
Size: 53 83 186 936 981 429 381 529
Cuts at: 3853 4235 4870 275
Size: 382 635 1451
Fragments arranged by size:
1451 981 936 635 529 429 382 381
186 83 53
BsaWI w'CCGG_w
Cuts at: 2039 2106 2520 4479 4877 5151 5298 5709 2039
Size: 67 414 1959 398 274 147 411 2376
Fragments arranged by size:
2376 1959 414 411 398 274 147 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2933 2963 1178
Size: 30 165 30 741 30 759 30 4261
Fragments arranged by size:
4261 759 741 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 2168 1186
Size: 939 43 5064
Fragments arranged by size:
5064 939 43
BseYI C'CCAG_C
Cuts at: 2032 2855 5249 5745 2032
Size: 823 2394 496 2333
Fragments arranged by size:
2394 2333 823 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 1163
Size: 573 345 5128
Fragments arranged by size:
5128 573 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 4107 4192 5263 2135
Size: 1972 85 1071 2918
Fragments arranged by size:
2918 1972 1071 85
BsmI GAATG_Cn'
Cuts at: 2057 3557 3650 2057
Size: 1500 93 4453
Fragments arranged by size:
4453 1500 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2190 2672 3129 3171 3365 4107
Size: 399 345 55 482 457 42 194 742
Cuts at: 4107 4192 5263 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 457 399 345 194
85 55 42
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3251 3334 3401 844
Size: 2407 83 67 3489
Fragments arranged by size:
3489 2407 83 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2686 3933 1394
Size: 429 863 1247 3507
Fragments arranged by size:
3507 1247 863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 3106 4690 4877 1700
Size: 110 229 1067 1584 187 2869
Fragments arranged by size:
2869 1584 1067 229 187 110
BsrGI T'GTAC_A
Cuts at: 96 2637 96
Size: 2541 3505
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6046
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstXI CCAn_nnnn'nTGG
Cuts at: 2398 2398
Size: 6046
BstYI r'GATC_y
Cuts at: 1972 2349 3376 3743 4128 4145 5597 5683 5695
Size: 377 1027 367 385 17 1452 86 12
Cuts at: 5695 6041 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1027 385 377 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2283 2283
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 2004 3081 513
Size: 552 939 1077 3478
Fragments arranged by size:
3478 1077 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 4356 4376 3562
Size: 794 20 5232
Fragments arranged by size:
5232 794 20
ClaI AT'CG_AT
Cuts at: 1554 2550 1554
Size: 996 5050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3214 3249 529
Size: 35 2650 35 3326
Fragments arranged by size:
3326 2650 35 35
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DraIII CAC_nnn'GTG
Cuts at: 3031 3031
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2560 2887 3493
Size: 54 1425 50 275 18 730 327 606
Cuts at: 3493 4382 6011 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 730 606 327 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3976 1353
Size: 2623 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4633 5007 5153 387
Size: 1064 138 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2133 2133
Size: 6046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2201 3134 4109 4706 5493 5761
Size: 393 539 30 933 975 597 787 268
Cuts at: 5761 1239
Size: 1524
Fragments arranged by size:
1524 975 933 787 597 539 393 268
30
EcoO109I rG'GnC_Cy
Cuts at: 2186 2187 2186
Size: 1 6045
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 2422 2576 5193 1678
Size: 744 154 2617 2531
Fragments arranged by size:
2617 2531 744 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2933 2965 4687 4719 4761
Size: 32 135 32 1361 32 1722 32 42
Cuts at: 4761 4793 1373
Size: 32 2626
Fragments arranged by size:
2626 1722 1361 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2488 2623 2983 3390 3636
Size: 988 281 289 261 135 360 407 246
Cuts at: 3636 669
Size: 3079
Fragments arranged by size:
3079 988 407 360 289 281 261 246
135
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 5160 5344 1305
Size: 1797 2058 184 2007
Fragments arranged by size:
2058 2007 1797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2562 2889 10
Size: 54 1425 343 730 327 3167
Fragments arranged by size:
3167 1425 730 343 327 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2780 3002 3071 4004 4363 4522
Size: 648 909 705 222 69 933 359 159
Cuts at: 4522 518
Size: 2042
Fragments arranged by size:
2042 933 909 705 648 359 222 159
69
MspA1I CmG'CkG
Cuts at: 1606 2263 2832 2842 3462 4139 5287 5532 1606
Size: 657 569 10 620 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 657 620 569 245 10
NaeI GCC'GGC
Cuts at: 3108 3108
Size: 6046
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4555
Fragments arranged by size:
4555 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NgoMIV G'CCGG_C
Cuts at: 3106 3106
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NsiI A_TGCA'T
Cuts at: 2057 2057
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 2407 2509 3404 4949 1132
Size: 97 1178 102 895 1545 2229
Fragments arranged by size:
2229 1545 1178 895 102 97
PasI CC'CwG_GG
Cuts at: 1348 2710 3040 1348
Size: 1362 330 4354
Fragments arranged by size:
4354 1362 330
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2569 1108
Size: 1461 4585
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PspOMI G'GGCC_C
Cuts at: 2186 2186
Size: 6046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2102 2134 2102
Size: 32 6014
PstI C_TGCA'G
Cuts at: 830 3398 830
Size: 2568 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 2263 2842 2263
Size: 579 5467
RsrII CG'GwC_CG
Cuts at: 2869 2869
Size: 6046
SacI G_AGCT'C
Cuts at: 2135 2135
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SexAI A'CCwGG_T
Cuts at: 2976 2976
Size: 6046
SfcI C'TryA_G
Cuts at: 826 1045 3394 4529 5210 5401 826
Size: 219 2349 1135 681 191 1471
Fragments arranged by size:
2349 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 3373 1872
Size: 1501 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2612 4150 5051 5313 820
Size: 197 926 669 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 669 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 2509 3404 1132
Size: 1377 895 3774
Fragments arranged by size:
3774 1377 895
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 2381 1517
Size: 864 5182
StyI C'CwwG_G
Cuts at: 513 1065 2004 2382 3485 513
Size: 552 939 378 1103 3074
Fragments arranged by size:
3074 1103 939 552 378
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4073 4090 4243 4428 4873 325
Size: 1255 2493 17 153 185 445 1498
Fragments arranged by size:
2493 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 3048 4292
Size: 274 80 33 51 1443 660 411 1244
Cuts at: 4292 6004 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1244 660 411 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6006 6038 6006
Size: 32 6014
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2245 3070 2146
Size: 99 825 5122
Fragments arranged by size:
5122 825 99
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 3371 1870
Size: 1501 4545
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2515 3325 3854 276
Size: 53 83 186 936 981 810 529 2468
Fragments arranged by size:
2468 981 936 810 529 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI Bsu36I EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI PacI PmlI PpuMI PspXI SacII
SanDI SapI SfiI SfoI SrfI SwaI