Restriction map for
pFN21AB8020
(Circular) (Six-base) MAPSORT of: pFN21AB8020.seq Check: 56 from: 1 to: 6775
pFN21AB8020 6775 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4118 1937
Size: 2181 4594
Acc65I G'GTAC_C
Cuts at: 2519 4096 2519
Size: 1577 5198
AclI AA'CG_TT
Cuts at: 1563 2380 4902 5275 1563
Size: 817 2522 373 3063
Fragments arranged by size:
3063 2522 817 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2840 3209 4838 6222 6490 1632
Size: 708 500 369 1629 1384 268 1917
Fragments arranged by size:
1917 1629 1384 708 500 369 268
AfeI AGC'GCT
Cuts at: 2647 2647
Size: 6775
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6578
AflIII A'CryG_T
Cuts at: 1203 5674 6651 1203
Size: 4471 977 1327
Fragments arranged by size:
4471 1327 977
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 5504 5504
Size: 6775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2948 1184
Size: 1764 5011
AlwNI CAG_nnn'CTG
Cuts at: 2564 4518 6090 2564
Size: 1954 1572 3249
Fragments arranged by size:
3249 1954 1572
ApaI G_GGCC'C
Cuts at: 2499 2499
Size: 6775
ApaLI G'TGCA_C
Cuts at: 4832 5988 4832
Size: 1156 5619
ApoI r'AATT_y
Cuts at: 1493 2709 3247 4084 4310 4462 6571 1493
Size: 1216 538 837 226 152 2109 1697
Fragments arranged by size:
2109 1697 1216 837 538 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5329 160
Size: 872 4297 1606
Fragments arranged by size:
4297 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 2901 4100 1870
Size: 73 958 1199 4545
Fragments arranged by size:
4545 1199 958 73
BamHI G'GATC_C
Cuts at: 4105 4472 4105
Size: 367 6408
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2519 4096 5551 618
Size: 325 217 627 732 1577 1455 1842
Fragments arranged by size:
1842 1577 1455 732 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2499 4094 1391
Size: 1108 1595 4072
Fragments arranged by size:
4072 1595 1108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4966 5000 1903
Size: 34 3029 34 3678
Fragments arranged by size:
3678 3029 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4667 5877 4667
Size: 1210 5565
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5386 136
Size: 107 122 71 631 605 3714 1525
Fragments arranged by size:
3714 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6770 6770
Size: 6775
BlpI GC'TnA_GC
Cuts at: 3146 4192 3146
Size: 1046 5729
Bme1580I G_kGCm'C
Cuts at: 1790 2499 3183 4836 5992 1790
Size: 709 684 1653 1156 2573
Fragments arranged by size:
2573 1653 1156 709 684
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4506 5464 442
Size: 416 53 83 399 24 3089 958 1753
Fragments arranged by size:
3089 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2439 2465 2514 2670 2862 5435 1239
Size: 1172 28 26 49 156 192 2573 2579
Fragments arranged by size:
2579 2573 1172 192 156 49 28 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2454 3683 4900 5765 6063 2454
Size: 1229 1217 865 298 3166
Fragments arranged by size:
3166 1229 1217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2838 5438 882
Size: 617 1339 2600 2219
Fragments arranged by size:
2600 2219 1339 617
BsaAI yAC'GTr
Cuts at: 493 1204 4566 493
Size: 711 3362 2702
Fragments arranged by size:
3362 2702 711
BsaBI GATnn'nnATC
Cuts at: 6769 6769
Size: 6775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4582 4964 5599 275
Size: 53 83 186 936 3049 382 635 1451
Fragments arranged by size:
3049 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2265 3202 5208 5606 5880 6027 6438 2265
Size: 937 2006 398 274 147 411 2602
Fragments arranged by size:
2602 2006 937 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2512 2542 3228 3258 1178
Size: 30 165 30 1109 30 686 30 4695
Fragments arranged by size:
4695 1109 686 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2041 2624 2654 3695 3716 1186
Size: 852 3 583 30 1041 21 4245
Fragments arranged by size:
4245 1041 852 583 30 21 3
BseYI C'CCAG_C
Cuts at: 2916 3276 3455 3779 5978 6474 2916
Size: 360 179 324 2199 496 3217
Fragments arranged by size:
3217 2199 496 360 324 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3984 1163
Size: 573 2248 3954
Fragments arranged by size:
3954 2248 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4225 4986 6014 1540
Size: 275 186 2224 761 1028 2301
Fragments arranged by size:
2301 2224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2977 4836 4921 5992 2977
Size: 1859 85 1071 3760
Fragments arranged by size:
3760 1859 1071 85
BsmI GAATG_Cn'
Cuts at: 4286 4379 4286
Size: 93 6682
BsmBI CGTCTCn'nnnn_
Cuts at: 2595 2817 2595
Size: 222 6553
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2977 3183 4094 4836 4921 5992
Size: 399 709 478 206 911 742 85 1071
Cuts at: 5992 1391
Size: 2174
Fragments arranged by size:
2174 1071 911 742 709 478 399 206
85
BspEI T'CCGG_A
Cuts at: 3202 6438 3202
Size: 3236 3539
BspHI T'CATG_A
Cuts at: 4664 6394 4664
Size: 1730 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2507 4130 844
Size: 1663 1623 3489
Fragments arranged by size:
3489 1663 1623
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 4662 1394
Size: 429 749 2090 3507
Fragments arranged by size:
3507 2090 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2814 2887 3160 3621 5270 5444 65
Size: 1183 1566 73 273 461 1649 174 1396
Fragments arranged by size:
1649 1566 1396 1183 461 273 174 73
BsrFI r'CCGG_y
Cuts at: 1700 1810 5419 5606 1700
Size: 110 3609 187 2869
Fragments arranged by size:
3609 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2115 96
Size: 2019 4756
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 3003 3528 4835 5847 3003
Size: 525 1307 1012 3931
Fragments arranged by size:
3931 1307 1012 525
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6775
BstBI TT'CG_AA
Cuts at: 5579 5579
Size: 6775
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6775
BstYI r'GATC_y
Cuts at: 1972 2509 3373 4105 4472 4857 4874 6326 6412
Size: 537 864 732 367 385 17 1452 86
Cuts at: 6412 6424 6770 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 864 732 537 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3514 3981 513
Size: 552 939 1510 467 3307
Fragments arranged by size:
3307 1510 939 552 467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6036
Fragments arranged by size:
6036 683 56
BtsI GCAGTG_nn'
Cuts at: 2565 2930 4291 5085 5105 2565
Size: 365 1361 794 20 4235
Fragments arranged by size:
4235 1361 794 365 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6740
DraI TTT'AAA
Cuts at: 4079 4079
Size: 6775
DraIII CAC_nnn'GTG
Cuts at: 2198 2198
Size: 6775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2587 5782 809
Size: 507 1271 3195 1802
Fragments arranged by size:
3195 1802 1271 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 3168 3960
Size: 54 1425 50 275 18 253 1085 792
Cuts at: 3960 4222 5111 6740 8
Size: 262 889 1629 43
Fragments arranged by size:
1629 1425 1085 889 792 275 262 253
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2059 2598 3003 3033 3858 4705 1353
Size: 706 539 405 30 825 847 3423
Fragments arranged by size:
3423 847 825 706 539 405 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5362 5736 5882 387
Size: 1064 138 3773 374 146 1280
Fragments arranged by size:
3773 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2411 2439 2465 2514 2670 2840
Size: 393 708 71 28 26 49 156 170
Cuts at: 2840 2862 3209 4838 5435 6222 6490 1239
Size: 22 347 1629 597 787 268 1524
Fragments arranged by size:
1629 1524 787 708 597 393 347 268
170 156 71 49 28 26 22
EcoRI G'AATT_C
Cuts at: 4084 4462 4084
Size: 378 6397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3044 3044
Size: 6775
FspI TGC'GCA
Cuts at: 5281 5281
Size: 6775
HaeII r_GCGC'y
Cuts at: 1678 2649 2962 5922 1678
Size: 971 313 2960 2531
Fragments arranged by size:
2960 2531 971 313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2981 3013 5416
Size: 32 135 32 443 32 934 32 2403
Cuts at: 5416 5448 5490 5522 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2403 934 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2863 3978 4119 4365 669
Size: 988 281 925 1115 141 246 3079
Fragments arranged by size:
3079 1115 988 925 281 246 141
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6775
HpaI GTT'AAC
Cuts at: 4365 4365
Size: 6775
KpnI G_GTAC'C
Cuts at: 2523 4100 2523
Size: 1577 5198
MfeI C'AATT_G
Cuts at: 3255 4374 3255
Size: 1119 5656
MluI A'CGCG_T
Cuts at: 6651 6651
Size: 6775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 3093 5889 6073 1305
Size: 936 852 2796 184 2007
Fragments arranged by size:
2796 2007 936 852 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2085 3170 10
Size: 54 1425 343 253 1085 3615
Fragments arranged by size:
3615 1425 1085 343 253 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2895 3494 3956 4733 5092 5251 518
Size: 648 1729 599 462 777 359 159 2042
Fragments arranged by size:
2042 1729 777 648 599 462 359 159
MspA1I CmG'CkG
Cuts at: 1606 4191 4868 6016 6261 1606
Size: 2585 677 1148 245 2120
Fragments arranged by size:
2585 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3514 513
Size: 552 939 1510 3774
Fragments arranged by size:
3774 1510 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6775
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6775
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NspI r_CATG'y
Cuts at: 1132 1229 3396 4133 5678 1132
Size: 97 2167 737 1545 2229
Fragments arranged by size:
2229 2167 1545 737 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6775
PciI A'CATG_T
Cuts at: 5674 5674
Size: 6775
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3515 1108
Size: 2407 4368
PfoI T'CCnGG_A
Cuts at: 2416 2790 2416
Size: 374 6401
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 6357 6389 4844
Size: 32 1481 32 5230
Fragments arranged by size:
5230 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 4345 4345
Size: 6775
PspOMI G'GGCC_C
Cuts at: 2495 2495
Size: 6775
PstI C_TGCA'G
Cuts at: 830 2181 2518 4127 830
Size: 1351 337 1609 3478
Fragments arranged by size:
3478 1609 1351 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SapI GCTCTTCn'nnn_
Cuts at: 3033 3033
Size: 6775
SbfI CC_TGCA'GG
Cuts at: 2518 4127 2518
Size: 1609 5166
ScaI AGT'ACT
Cuts at: 3715 5023 6735 3715
Size: 1308 1712 3755
Fragments arranged by size:
3755 1712 1308
SfcI C'TryA_G
Cuts at: 826 1045 2177 2514 4123 5258 5939 6130 826
Size: 219 1132 337 1609 1135 681 191 1471
Fragments arranged by size:
1609 1471 1135 1132 681 337 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 4102 1872
Size: 2230 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2469 3662 4879 5780 6042 820
Size: 197 926 526 1193 1217 901 262 1553
Fragments arranged by size:
1553 1217 1193 926 901 526 262 197
SnaBI TAC'GTA
Cuts at: 493 4566 493
Size: 4073 2702
SpeI A'CTAG_T
Cuts at: 152 2459 4003 152
Size: 2307 1544 2924
Fragments arranged by size:
2924 2307 1544
SphI G_CATG'C
Cuts at: 1132 4133 1132
Size: 3001 3774
SspI AAT'ATT
Cuts at: 5 52 4699 5
Size: 47 4647 2081
Fragments arranged by size:
4647 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6775
StyI C'CwwG_G
Cuts at: 513 1065 2004 3439 3514 4214 513
Size: 552 939 1435 75 700 3074
Fragments arranged by size:
3074 1435 939 700 552 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2706 2744 3174 4802 4819 4972 5157
Size: 1255 1126 38 430 1628 17 153 185
Cuts at: 5157 5602 325
Size: 445 1498
Fragments arranged by size:
1628 1498 1255 1126 445 430 185 153
38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 3713 5021
Size: 274 80 33 51 1443 138 1598 1308
Cuts at: 5021 6733 96
Size: 1712 138
Fragments arranged by size:
1712 1598 1443 1308 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3228 3260 3651 3683 6735 6767 3228
Size: 32 391 32 3052 32 3236
Fragments arranged by size:
3236 3052 391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2147 1316
Size: 831 5944
XbaI T'CTAG_A
Cuts at: 4111 4111
Size: 6775
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6775
XmaI C'CCGG_G
Cuts at: 1870 4100 1870
Size: 2230 4545
XmnI GAAnn'nnTTC
Cuts at: 2249 4904 2249
Size: 2655 4120
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI