Restriction map for
pFN21AB8019
(Circular) (Six-base) MAPSORT of: pFN21AB8019.seq Check: 9164 from: 1 to: 6715
pFN21AB8019 6715 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2902 2902
Size: 6715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4525 278
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4058 1937
Size: 2121 4594
Acc65I G'GTAC_C
Cuts at: 4036 4036
Size: 6715
AclI AA'CG_TT
Cuts at: 1563 4842 5215 1563
Size: 3279 373 3063
Fragments arranged by size:
3279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2698 3374 4778 6162 6430 1632
Size: 1066 676 1404 1384 268 1917
Fragments arranged by size:
1917 1404 1384 1066 676 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6518
AflIII A'CryG_T
Cuts at: 1203 2901 3298 5614 6591 1203
Size: 1698 397 2316 977 1327
Fragments arranged by size:
2316 1698 1327 977 397
AgeI A'CCGG_T
Cuts at: 5546 5546
Size: 6715
AhdI GACnn_n'nnGTC
Cuts at: 2536 3914 5444 2536
Size: 1378 1530 3807
Fragments arranged by size:
3807 1530 1378
AleI CACnn'nnGTG
Cuts at: 1166 3299 1166
Size: 2133 4582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6715
AlwNI CAG_nnn'CTG
Cuts at: 4458 6030 4458
Size: 1572 5143
ApaI G_GGCC'C
Cuts at: 2994 3685 2994
Size: 691 6024
ApaLI G'TGCA_C
Cuts at: 2129 4772 5928 2129
Size: 2643 1156 2916
Fragments arranged by size:
2916 2643 1156
ApoI r'AATT_y
Cuts at: 1493 2141 4024 4250 4402 6511 1493
Size: 648 1883 226 152 2109 1697
Fragments arranged by size:
2109 1883 1697 648 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5269 160
Size: 872 4237 1606
Fragments arranged by size:
4237 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 3208 3556 3595 3685 3844 3902
Size: 73 135 1130 348 39 90 159 58
Cuts at: 3902 4040 1870
Size: 138 4545
Fragments arranged by size:
4545 1130 348 159 138 135 90 73
58 39
BamHI G'GATC_C
Cuts at: 4045 4412 4045
Size: 367 6348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2487 3148 3751 4036 5491
Size: 325 217 627 700 661 603 285 1455
Cuts at: 5491 618
Size: 1842
Fragments arranged by size:
1842 1455 700 661 627 603 325 285
217
BanII G_rGCy'C
Cuts at: 1391 2895 2994 3124 3685 3852 4034 1391
Size: 1504 99 130 561 167 182 4072
Fragments arranged by size:
4072 1504 561 182 167 130 99
BbeI G_GCGC'C
Cuts at: 3152 3152
Size: 6715
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3409 928
Size: 971 1510 4234
Fragments arranged by size:
4234 1510 971
BbvCI CC'TCA_GC
Cuts at: 2256 2549 2256
Size: 293 6422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2669 2703 2782 2816 3421 3455 4906
Size: 34 732 34 79 34 605 34 1451
Cuts at: 4906 4940 1903
Size: 34 3678
Fragments arranged by size:
3678 1451 732 605 79 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5817 4607
Size: 1210 5505
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6715
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5326 136
Size: 107 122 71 631 605 3654 1525
Fragments arranged by size:
3654 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2190 6710 2190
Size: 4520 2195
BlpI GC'TnA_GC
Cuts at: 2114 2612 3800 4132 2114
Size: 498 1188 332 4697
Fragments arranged by size:
4697 1188 498 332
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2291 2449 2490 2994 3685 4776 5932
Size: 343 158 158 41 504 691 1091 1156
Cuts at: 5932 1790
Size: 2573
Fragments arranged by size:
2573 1156 1091 691 504 343 158 158
41
BmgBI CAC'GTC
Cuts at: 1318 2923 3277 1318
Size: 1605 354 4756
Fragments arranged by size:
4756 1605 354
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4446 5404 442
Size: 416 53 83 399 24 3029 958 1753
Fragments arranged by size:
3029 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3236 3451 5375 1239
Size: 1997 215 1924 2579
Fragments arranged by size:
2579 1997 1924 215
Bpu10I CC'TnA_GC
Cuts at: 2256 2549 2256
Size: 293 6422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2582 3224 4840 5705 6003 2342
Size: 240 642 1616 865 298 3054
Fragments arranged by size:
3054 1616 865 642 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 2242 2493 5378 882
Size: 617 507 236 251 2885 2219
Fragments arranged by size:
2885 2219 617 507 251 236
BsaAI yAC'GTr
Cuts at: 493 1204 3301 4506 493
Size: 711 2097 1205 2702
Fragments arranged by size:
2702 2097 1205 711
BsaBI GATnn'nnATC
Cuts at: 3248 6709 3248
Size: 3461 3254
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 2452 3149 4522
Size: 53 83 186 936 515 404 697 1373
Cuts at: 4522 4904 5539 275
Size: 382 635 1451
Fragments arranged by size:
1451 1373 936 697 635 515 404 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2652 5148 5546 5820 5967 6378 2652
Size: 2496 398 274 147 411 2989
Fragments arranged by size:
2989 2496 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 2690 2720 2831
Size: 30 165 30 633 30 624 30 111
Cuts at: 2831 2861 3449 3479 1178
Size: 30 588 30 4414
Fragments arranged by size:
4414 633 624 588 165 111 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2057 2201 2480 2698 1186
Size: 868 3 144 279 218 5203
Fragments arranged by size:
5203 868 279 218 144 3
BseYI C'CCAG_C
Cuts at: 3018 3719 5918 6414 3018
Size: 701 2199 496 3319
Fragments arranged by size:
3319 2199 701 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6142
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2230 2821 2884 3229 3792 4165
Size: 275 186 229 591 63 345 563 373
Cuts at: 4165 4926 5954 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 591 563 373 345 275
229 186 63
BsiHKAI G_wGCw'C
Cuts at: 2115 2133 2266 2636 4776 4861 5932 2115
Size: 18 133 370 2140 85 1071 2898
Fragments arranged by size:
2898 2140 1071 370 133 85 18
BsmI GAATG_Cn'
Cuts at: 4226 4319 4226
Size: 93 6622
BsmBI CGTCTCn'nnnn_
Cuts at: 2039 2820 2039
Size: 781 5934
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2133 2266 2291 2449 2490 2636
Size: 399 325 18 133 25 158 41 146
Cuts at: 2636 2895 2994 3124 3685 3852 4034 4776 4861
Size: 259 99 130 561 167 182 742 85
Cuts at: 4861 5932 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 561 399 325 259 182
167 158 146 133 130 99 85 41
25 18
BspEI T'CCGG_A
Cuts at: 2652 6378 2652
Size: 3726 2989
BspHI T'CATG_A
Cuts at: 2853 4604 6334 2853
Size: 1751 1730 3234
Fragments arranged by size:
3234 1751 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2902 4070 844
Size: 1472 586 1168 3489
Fragments arranged by size:
3489 1472 1168 586
BsrBI CCG'CTC
Cuts at: 1394 1823 2326 3319 4602 1394
Size: 429 503 993 1283 3507
Fragments arranged by size:
3507 1283 993 503 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2950 5210 5384 65
Size: 1183 1702 2260 174 1396
Fragments arranged by size:
2260 1702 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2433 2667 3791 5359 5546 1700
Size: 110 311 312 234 1124 1568 187 2869
Fragments arranged by size:
2869 1568 1124 312 311 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 3291 96
Size: 3195 3520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6715
BssSI C'ACGA_G
Cuts at: 2602 4775 5787 2602
Size: 2173 1012 3530
Fragments arranged by size:
3530 2173 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6715
BstBI TT'CG_AA
Cuts at: 5519 5519
Size: 6715
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6715
BstYI r'GATC_y
Cuts at: 1972 2190 3219 3424 4045 4412 4797 4814 6266
Size: 218 1029 205 621 367 385 17 1452
Cuts at: 6266 6352 6364 6710 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1029 621 385 367 346 218
205 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2157 2421 2622 2821 3053
Size: 552 939 66 87 264 201 199 232
Cuts at: 3053 3805 513
Size: 752 3423
Fragments arranged by size:
3423 939 752 552 264 232 201 199
87 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3235 487
Size: 683 56 2009 3967
Fragments arranged by size:
3967 2009 683 56
BtsI GCAGTG_nn'
Cuts at: 3410 3661 3733 3739 4231 5025 5045 3410
Size: 251 72 6 492 794 20 5080
Fragments arranged by size:
5080 794 492 251 72 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2803 2838 3637 3672 529
Size: 35 2239 35 799 35 3572
Fragments arranged by size:
3572 2239 799 35 35 35
DraI TTT'AAA
Cuts at: 4019 4019
Size: 6715
DraIII CAC_nnn'GTG
Cuts at: 3758 3758
Size: 6715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5722 809
Size: 507 4406 1802
Fragments arranged by size:
4406 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2227 2376
Size: 54 1425 50 275 18 264 133 149
Cuts at: 2376 2871 2881 3009 3789 3793 4162 5051 6680
Size: 495 10 128 780 4 369 889 1629
Cuts at: 6680 8
Size: 43
Fragments arranged by size:
1629 1425 889 780 495 369 275 264
149 133 128 54 50 43 18 10
4
EagI C'GGCC_G
Cuts at: 1537 1812 2227 2881 3789 4162 1537
Size: 275 415 654 908 373 4090
Fragments arranged by size:
4090 908 654 415 373 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3138 3158 4645 1353
Size: 1785 20 1487 3423
Fragments arranged by size:
3423 1785 1487 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2686 2711 3287 5302 5676
Size: 1064 138 435 662 25 576 2015 374
Cuts at: 5676 5822 387
Size: 146 1280
Fragments arranged by size:
2015 1280 1064 662 576 435 374 146
138 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2698 3236 3374 3451 4778 5375 6162
Size: 393 1066 538 138 77 1327 597 787
Cuts at: 6162 6430 1239
Size: 268 1524
Fragments arranged by size:
1524 1327 1066 787 597 538 393 268
138 77
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2690 2255
Size: 435 6280
EcoO109I rG'GnC_Cy
Cuts at: 2280 2990 3022 3560 3592 3682 3908 2280
Size: 710 32 538 32 90 226 5087
Fragments arranged by size:
5087 710 538 226 90 32 32
EcoRI G'AATT_C
Cuts at: 4024 4402 4024
Size: 378 6337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3379 3409 3379
Size: 30 6685
FseI GG_CCGG'CC
Cuts at: 3795 3795
Size: 6715
FspI TGC'GCA
Cuts at: 5221 5221
Size: 6715
HaeII r_GCGC'y
Cuts at: 1678 2686 3152 3180 5862 1678
Size: 1008 466 28 2682 2531
Fragments arranged by size:
2682 2531 1008 466 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2522 2549 2554
Size: 32 135 32 692 32 226 27 5
Cuts at: 2554 2581 2690 2722 3204 3236 3466 3498 3900
Size: 27 109 32 482 32 230 32 402
Cuts at: 3900 3932 5356 5388 5430 5462 1373
Size: 32 1424 32 42 32 2626
Fragments arranged by size:
2626 1424 692 482 402 230 226 135
109 42 32 32 32 32 32 32
32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3382 4059 4305 669
Size: 988 281 1444 677 246 3079
Fragments arranged by size:
3079 1444 988 677 281 246
HindIII A'AGCT_T
Cuts at: 748 2210 748
Size: 1462 5253
HpaI GTT'AAC
Cuts at: 4305 4305
Size: 6715
KasI G'GCGC_C
Cuts at: 3148 3148
Size: 6715
KpnI G_GTAC'C
Cuts at: 4040 4040
Size: 6715
MfeI C'AATT_G
Cuts at: 4314 4314
Size: 6715
MluI A'CGCG_T
Cuts at: 6591 6591
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5829 6013 1305
Size: 4524 184 2007
Fragments arranged by size:
4524 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 3011 10
Size: 54 1425 343 546 633 3714
Fragments arranged by size:
3714 1425 633 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 2370 3299 4673 5032 5191 518
Size: 648 1105 99 929 1374 359 159 2042
Fragments arranged by size:
2042 1374 1105 929 648 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2876 3154 3199 3502 3615 3774 4131 4808
Size: 1270 278 45 303 113 159 357 677
Cuts at: 4808 5956 6201 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1270 1148 677 357 303 278 245
159 113 45
NaeI GCC'GGC
Cuts at: 2669 3793 2669
Size: 1124 5591
NarI GG'CG_CC
Cuts at: 3149 3149
Size: 6715
NcoI C'CATG_G
Cuts at: 513 1065 2004 2157 2421 513
Size: 552 939 153 264 4807
Fragments arranged by size:
4807 939 552 264 153
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6715
NgoMIV G'CCGG_C
Cuts at: 2667 3791 2667
Size: 1124 5591
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6715
NotI GC'GGCC_GC
Cuts at: 4162 4162
Size: 6715
NspI r_CATG'y
Cuts at: 1132 1229 2905 3235 4073 5618 1132
Size: 97 1676 330 838 1545 2229
Fragments arranged by size:
2229 1676 1545 838 330 97
PasI CC'CwG_GG
Cuts at: 1348 2284 1348
Size: 936 5779
PciI A'CATG_T
Cuts at: 2901 5614 2901
Size: 2713 4002
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3018 1108
Size: 1910 4805
PfoI T'CCnGG_A
Cuts at: 3918 3918
Size: 6715
PmeI GTTT'AAAC
Cuts at: 4019 4019
Size: 6715
PmlI CAC'GTG
Cuts at: 3301 3301
Size: 6715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4784 4816 6297 6329 4784
Size: 32 1481 32 5170
Fragments arranged by size:
5170 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2280 3022 3560 3592 3908 2280
Size: 742 538 32 316 5087
Fragments arranged by size:
5087 742 538 316 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6715
PsiI TTA'TAA
Cuts at: 4285 4285
Size: 6715
PspOMI G'GGCC_C
Cuts at: 2990 3681 2990
Size: 691 6024
PstI C_TGCA'G
Cuts at: 830 2913 3434 4067 830
Size: 2083 521 633 3478
Fragments arranged by size:
3478 2083 633 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6715
PvuII CAG'CTG
Cuts at: 3199 3774 3199
Size: 575 6140
RsrII CG'GwC_CG
Cuts at: 2698 2698
Size: 6715
SalI G'TCGA_C
Cuts at: 1936 4057 1936
Size: 2121 4594
SanDI GG'GwC_CC
Cuts at: 2280 3022 3560 3908 2280
Size: 742 538 348 5087
Fragments arranged by size:
5087 742 538 348
SbfI CC_TGCA'GG
Cuts at: 4067 4067
Size: 6715
ScaI AGT'ACT
Cuts at: 4963 6675 4963
Size: 1712 5003
SexAI A'CCwGG_T
Cuts at: 2055 2055
Size: 6715
SfcI C'TryA_G
Cuts at: 826 1045 2909 3430 4063 5198 5879 6070 826
Size: 219 1864 521 633 1135 681 191 1471
Fragments arranged by size:
1864 1471 1135 681 633 521 219 191
SfoI GGC'GCC
Cuts at: 3150 3150
Size: 6715
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
SgrAI Cr'CCGG_yG
Cuts at: 1700 2433 2667 1700
Size: 733 234 5748
Fragments arranged by size:
5748 733 234
SmaI CCC'GGG
Cuts at: 1872 3558 3597 3687 3846 3904 4042 1872
Size: 1686 39 90 159 58 138 4545
Fragments arranged by size:
4545 1686 159 138 90 58 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 2561 3239 4819 5720 5982
Size: 197 926 378 240 678 1580 901 262
Cuts at: 5982 820
Size: 1553
Fragments arranged by size:
1580 1553 926 901 678 378 262 240
197
SnaBI TAC'GTA
Cuts at: 493 4506 493
Size: 4013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6715
SphI G_CATG'C
Cuts at: 1132 4073 1132
Size: 2941 3774
SspI AAT'ATT
Cuts at: 5 52 4639 5
Size: 47 4587 2081
Fragments arranged by size:
4587 2081 47
StuI AGG'CCT
Cuts at: 1517 3830 3878 1517
Size: 2313 48 4354
Fragments arranged by size:
4354 2313 48
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2421 2492 3873 4154 513
Size: 552 939 153 264 71 1381 281 3074
Fragments arranged by size:
3074 1381 939 552 281 264 153 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2505 2517 4742 4759 4912 5097 5542
Size: 1255 925 12 2225 17 153 185 445
Cuts at: 5542 325
Size: 1498
Fragments arranged by size:
2225 1498 1255 925 445 185 153 17
12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3291 4961 6673
Size: 274 80 33 51 1443 1314 1670 1712
Cuts at: 6673 96
Size: 138
Fragments arranged by size:
1712 1670 1443 1314 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3558 3590 6675 6707 3558
Size: 32 3085 32 3566
Fragments arranged by size:
3566 3085 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6715
XbaI T'CTAG_A
Cuts at: 4051 4051
Size: 6715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 3803 2575
Size: 1228 5487
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6715
XmaI C'CCGG_G
Cuts at: 1870 3556 3595 3685 3844 3902 4040 1870
Size: 1686 39 90 159 58 138 4545
Fragments arranged by size:
4545 1686 159 138 90 58 39
XmnI GAAnn'nnTTC
Cuts at: 4844 4844
Size: 6715
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4523 276
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FspAI NruI NsiI
PacI PspXI PsrI SacI SacII SapI SfiI SrfI
SwaI