Restriction map for
pFN21AB8017
(Circular) (Six-base) MAPSORT of: pFN21AB8017.seq Check: 8240 from: 1 to: 6646
pFN21AB8017 6646 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2487 3855 2487
Size: 1368 5278
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2137 2330 4709 6093 6361 1632
Size: 505 193 2379 1384 268 1917
Fragments arranged by size:
2379 1917 1384 505 268 193
AfeI AGC'GCT
Cuts at: 3521 3521
Size: 6646
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6449
AflIII A'CryG_T
Cuts at: 1203 2342 5545 6522 1203
Size: 1139 3203 977 1327
Fragments arranged by size:
3203 1327 1139 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 2540 5375 2540
Size: 2835 3811
AleI CACnn'nnGTG
Cuts at: 1166 2381 2498 3530 1166
Size: 1215 117 1032 4282
Fragments arranged by size:
4282 1215 1032 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6646
AlwNI CAG_nnn'CTG
Cuts at: 2125 2455 3653 4389 5961 2125
Size: 330 1198 736 1572 2810
Fragments arranged by size:
2810 1572 1198 736 330
ApaI G_GGCC'C
Cuts at: 2239 3860 2239
Size: 1621 5025
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 2647 3955 4181 4333 6442 1493
Size: 1154 1308 226 152 2109 1697
Fragments arranged by size:
2109 1697 1308 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 2321 2765 3733 3776 3971 1870
Size: 73 378 444 968 43 195 4545
Fragments arranged by size:
4545 968 444 378 195 73 43
BamHI G'GATC_C
Cuts at: 2617 3976 4343 2617
Size: 1359 367 4920
Fragments arranged by size:
4920 1359 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 3553 3967 5422 618
Size: 325 217 627 792 974 414 1455 1842
Fragments arranged by size:
1842 1455 974 792 627 414 325 217
BanII G_rGCy'C
Cuts at: 1391 2239 2636 2828 2933 3860 3965 1391
Size: 848 397 192 105 927 105 4072
Fragments arranged by size:
4072 927 848 397 192 105 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3476 928
Size: 971 1577 4098
Fragments arranged by size:
4098 1577 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4837 4871 1903
Size: 34 2900 34 3678
Fragments arranged by size:
3678 2900 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4538 5748 4538
Size: 1210 5436
BclI T'GATC_A
Cuts at: 1548 3564 1548
Size: 2016 4630
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2571 5257 136
Size: 107 122 71 631 605 899 2686 1525
Fragments arranged by size:
2686 1525 899 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6641 6641
Size: 6646
BlpI GC'TnA_GC
Cuts at: 4063 4063
Size: 6646
Bme1580I G_kGCm'C
Cuts at: 1790 2239 2412 2709 3860 4707 5863 1790
Size: 449 173 297 1151 847 1156 2573
Fragments arranged by size:
2573 1156 1151 847 449 297 173
BmgBI CAC'GTC
Cuts at: 1318 2659 1318
Size: 1341 5305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2242 2798 2936
Size: 416 53 83 399 24 825 556 138
Cuts at: 2936 3863 4377 5335 442
Size: 927 514 958 1753
Fragments arranged by size:
1753 958 927 825 556 514 416 399
138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 2102 2738 3836 3887 5306 1239
Size: 814 49 636 1098 51 1419 2579
Fragments arranged by size:
2579 1419 1098 814 636 51 49
Bpu10I CC'TnA_GC
Cuts at: 2822 2822
Size: 6646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2905 3134 3179 3482 4771 5636 5934 2905
Size: 229 45 303 1289 865 298 3617
Fragments arranged by size:
3617 1289 865 303 298 229 45
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2658 2804 2913 3125 3375 3812 3887
Size: 617 1159 146 109 212 250 437 75
Cuts at: 3887 5309 882
Size: 1422 2219
Fragments arranged by size:
2219 1422 1159 617 437 250 212 146
109 75
BsaAI yAC'GTr
Cuts at: 493 1204 2343 4437 493
Size: 711 1139 2094 2702
Fragments arranged by size:
2702 2094 1139 711
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4453 4835 5470 275
Size: 53 83 186 936 2920 382 635 1451
Fragments arranged by size:
2920 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2667 3117 5079 5477 5751 5898 6309 2667
Size: 450 1962 398 274 147 411 3004
Fragments arranged by size:
3004 1962 450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2297 2327 3344 3374 3404
Size: 30 165 30 894 30 1017 30 30
Cuts at: 3404 1178
Size: 4420
Fragments arranged by size:
4420 1017 894 165 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2902 3143 1186
Size: 1716 241 4689
Fragments arranged by size:
4689 1716 241
BseYI C'CCAG_C
Cuts at: 2300 2509 2800 2843 2943 3037 5849 6345 2300
Size: 209 291 43 100 94 2812 496 2601
Fragments arranged by size:
2812 2601 496 291 209 100 94 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6073
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3011 3574 4096 4857 5885 1540
Size: 275 186 1010 563 522 761 1028 2301
Fragments arranged by size:
2301 1028 1010 761 563 522 275 186
BsiHKAI G_wGCw'C
Cuts at: 2267 2391 2589 2828 3195 3213 4707 4792 5863
Size: 124 198 239 367 18 1494 85 1071
Cuts at: 5863 2267
Size: 3050
Fragments arranged by size:
3050 1494 1071 367 239 198 124 85
18
BsmI GAATG_Cn'
Cuts at: 4157 4250 4157
Size: 93 6553
BsmBI CGTCTCn'nnnn_
Cuts at: 3458 3458
Size: 6646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2267 2391 2412 2589 2636 2709
Size: 399 449 28 124 21 177 47 73
Cuts at: 2709 2828 2933 3195 3213 3860 3965 4707 4792
Size: 119 105 262 18 647 105 742 85
Cuts at: 4792 5863 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 647 449 399 262 177
124 119 105 105 85 73 47 28
21 18
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 2733 3669 3855 4001 844
Size: 1643 246 936 186 146 3489
Fragments arranged by size:
3489 1643 936 246 186 146
BsrBI CCG'CTC
Cuts at: 1394 1823 3915 4533 1394
Size: 429 2092 618 3507
Fragments arranged by size:
3507 2092 618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5141 5315 65
Size: 1183 3893 174 1396
Fragments arranged by size:
3893 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2988 3839 3900 5290 5477 1700
Size: 110 1178 851 61 1390 187 2869
Fragments arranged by size:
2869 1390 1178 851 187 110 61
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 4706 5718 4706
Size: 1012 5634
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2455 3048 794
Size: 1661 593 4392
Fragments arranged by size:
4392 1661 593
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 2431 1184
Size: 1247 5399
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 6646
BstYI r'GATC_y
Cuts at: 1972 2097 2451 2617 2670 3976 4343 4728 4745
Size: 125 354 166 53 1306 367 385 17
Cuts at: 4745 6197 6283 6295 6641 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1306 385 367 354 346 166
125 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 3480 3933 3480
Size: 453 6193
BtgI C'CryG_G
Cuts at: 513 1065 2257 2794 3025 3302 3528 3672 513
Size: 552 1192 537 231 277 226 144 3487
Fragments arranged by size:
3487 1192 552 537 277 231 226 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3184 3319 487
Size: 683 56 1958 135 3814
Fragments arranged by size:
3814 1958 683 135 56
BtsI GCAGTG_nn'
Cuts at: 2176 2410 4162 4956 4976 2176
Size: 234 1752 794 20 3846
Fragments arranged by size:
3846 1752 794 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6611
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DraIII CAC_nnn'GTG
Cuts at: 3080 3080
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2107 2990
Size: 54 1425 50 275 18 259 18 883
Cuts at: 2990 3008 3055 3571 3837 3841 4093 4982 6611
Size: 18 47 516 266 4 252 889 1629
Cuts at: 6611 8
Size: 43
Fragments arranged by size:
1629 1425 889 883 516 275 266 259
252 54 50 47 43 18 18 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 3008 3571 4093 1537
Size: 275 1196 563 522 4090
Fragments arranged by size:
4090 1196 563 522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 3776 4576 1353
Size: 866 1557 800 3423
Fragments arranged by size:
3423 1557 866 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5233 5607 5753 387
Size: 1064 138 3644 374 146 1280
Fragments arranged by size:
3644 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2826 2826
Size: 6646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2102 2137 2330 2738 3836 3887
Size: 393 421 49 35 193 408 1098 51
Cuts at: 3887 4709 5306 6093 6361 1239
Size: 822 597 787 268 1524
Fragments arranged by size:
1524 1098 822 787 597 421 408 393
268 193 51 49 35
EcoNI CCTnn'n_nnAGG
Cuts at: 3152 3152
Size: 6646
EcoO109I rG'GnC_Cy
Cuts at: 2122 2349 2804 2953 3484 3937 2122
Size: 227 455 149 531 453 4831
Fragments arranged by size:
4831 531 455 453 227 149
EcoRI G'AATT_C
Cuts at: 2647 3955 4333 2647
Size: 1308 378 4960
Fragments arranged by size:
4960 1308 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 6646
FseI GG_CCGG'CC
Cuts at: 3843 3843
Size: 6646
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 2919 3523 5793 1678
Size: 1241 604 2270 2531
Fragments arranged by size:
2531 2270 1241 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2526 2558 2655 2687 3323
Size: 32 135 32 954 32 97 32 636
Cuts at: 3323 3355 3374 3406 3587 3619 5287 5319 5361
Size: 32 19 32 181 32 1668 32 42
Cuts at: 5361 5393 1373
Size: 32 2626
Fragments arranged by size:
2626 1668 954 636 181 135 97 42
32 32 32 32 32 32 32 32
32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2013 3990 4236 669
Size: 988 281 75 1977 246 3079
Fragments arranged by size:
3079 1977 988 281 246 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 4245 4245
Size: 6646
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2641 2994 5760 5944 1305
Size: 1336 353 2766 184 2007
Fragments arranged by size:
2766 2007 1336 353 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2109 10
Size: 54 1425 343 277 4547
Fragments arranged by size:
4547 1425 343 277 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2498 3206 3530 3752 4604 4963
Size: 648 1215 117 708 324 222 852 359
Cuts at: 4963 5122 518
Size: 159 2042
Fragments arranged by size:
2042 1215 852 708 648 359 324 222
159 117
MspA1I CmG'CkG
Cuts at: 1606 2758 2847 3865 3874 4062 4739 5887 6132
Size: 1152 89 1018 9 188 677 1148 245
Cuts at: 6132 1606
Size: 2120
Fragments arranged by size:
2120 1152 1148 1018 677 245 188 89
9
NaeI GCC'GGC
Cuts at: 2990 3841 2990
Size: 851 5795
NcoI C'CATG_G
Cuts at: 513 1065 2257 3302 3672 513
Size: 552 1192 1045 370 3487
Fragments arranged by size:
3487 1192 1045 552 370
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NgoMIV G'CCGG_C
Cuts at: 2988 3839 2988
Size: 851 5795
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 3832 4004 5549 1132
Size: 97 2603 172 1545 2229
Fragments arranged by size:
2603 2229 1545 172 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6646
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 2614 2800 1108
Size: 1320 186 186 4954
Fragments arranged by size:
4954 1320 186 186
PfoI T'CCnGG_A
Cuts at: 3702 3702
Size: 6646
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PmlI CAC'GTG
Cuts at: 2343 2343
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2349 2953 3937 2349
Size: 604 984 5058
Fragments arranged by size:
5058 984 604
PshAI GACnn'nnGTC
Cuts at: 1613 2438 2958 1613
Size: 825 520 5301
Fragments arranged by size:
5301 825 520
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PspOMI G'GGCC_C
Cuts at: 2235 3856 2235
Size: 1621 5025
PspXI vC'TCGA_Gb
Cuts at: 2765 2765
Size: 6646
PstI C_TGCA'G
Cuts at: 830 2171 2897 3998 830
Size: 1341 726 1101 3478
Fragments arranged by size:
3478 1341 1101 726
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
PvuII CAG'CTG
Cuts at: 2758 3865 3874 2758
Size: 1107 9 5530
Fragments arranged by size:
5530 1107 9
SacI G_AGCT'C
Cuts at: 2828 2828
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SapI GCTCTTCn'nnn_
Cuts at: 2219 2219
Size: 6646
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 2192 4894 6606 2192
Size: 2702 1712 2232
Fragments arranged by size:
2702 2232 1712
SfcI C'TryA_G
Cuts at: 826 1045 2167 2893 3994 5129 5810 6001 826
Size: 219 1122 726 1101 1135 681 191 1471
Fragments arranged by size:
1471 1135 1122 1101 726 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 2884 3149 3194 3497 4750
Size: 197 926 822 119 265 45 303 1253
Cuts at: 4750 5651 5913 820
Size: 901 262 1553
Fragments arranged by size:
1553 1253 926 901 822 303 265 262
197 119 45
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 3832 4004 1132
Size: 2700 172 3774
Fragments arranged by size:
3774 2700 172
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6646
StyI C'CwwG_G
Cuts at: 513 1065 2257 2280 3302 3327 3672 4085 513
Size: 552 1192 23 1022 25 345 413 3074
Fragments arranged by size:
3074 1192 1022 552 413 345 25 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2250 4673 4690 4843 5028 5473 325
Size: 1255 670 2423 17 153 185 445 1498
Fragments arranged by size:
2423 1498 1255 670 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 2400 3246
Size: 274 80 33 51 1443 213 210 846
Cuts at: 3246 4892 6604 96
Size: 1646 1712 138
Fragments arranged by size:
1712 1646 1443 846 274 213 210 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 6606 6638 2583
Size: 32 3991 32 2591
Fragments arranged by size:
3991 2591 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XhoI C'TCGA_G
Cuts at: 1943 2765 1943
Size: 822 5824
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstZ17I EcoRV FspAI KasI NarI NruI NsiI
PacI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI