Restriction map for
pFN21AB8012
(Circular) (Six-base) MAPSORT of: pFN21AB8012.seq Check: 7862 from: 1 to: 6829
pFN21AB8012 6829 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3466 3466
Size: 6829
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4172 1937
Size: 2235 4594
Acc65I G'GTAC_C
Cuts at: 4150 4150
Size: 6829
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3017 4118 4892 6276 6544 1632
Size: 1385 1101 774 1384 268 1917
Fragments arranged by size:
1917 1385 1384 1101 774 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 3438 3855 3903 5728 6705 1203
Size: 2235 417 48 1825 977 1327
Fragments arranged by size:
2235 1825 1327 977 417 48
AgeI A'CCGG_T
Cuts at: 5660 5660
Size: 6829
AhdI GACnn_n'nnGTC
Cuts at: 3238 3738 5558 3238
Size: 500 1820 4509
Fragments arranged by size:
4509 1820 500
AleI CACnn'nnGTG
Cuts at: 1166 2135 1166
Size: 969 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6829
AlwNI CAG_nnn'CTG
Cuts at: 2195 3325 3944 4084 4572 6144 2195
Size: 1130 619 140 488 1572 2880
Fragments arranged by size:
2880 1572 1130 619 488 140
ApaI G_GGCC'C
Cuts at: 2333 3694 4120 2333
Size: 1361 426 5042
Fragments arranged by size:
5042 1361 426
ApaLI G'TGCA_C
Cuts at: 4886 6042 4886
Size: 1156 5673
ApoI r'AATT_y
Cuts at: 1493 2948 3590 4138 4364 4516 6625 1493
Size: 1455 642 548 226 152 2109 1697
Fragments arranged by size:
2109 1697 1455 642 548 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2040 3684 5383 160
Size: 872 1008 1644 1699 1606
Fragments arranged by size:
1699 1644 1606 1008 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 2601 3695 4154 1870
Size: 73 658 1094 459 4545
Fragments arranged by size:
4545 1094 658 459 73
AvrII C'CTAG_G
Cuts at: 3127 3127
Size: 6829
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2336 2369 2336
Size: 33 6796
BamHI G'GATC_C
Cuts at: 4159 4526 4159
Size: 367 6462
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 3454 4150 5605 618
Size: 325 217 627 533 1134 696 1455 1842
Fragments arranged by size:
1842 1455 1134 696 627 533 325 217
BanII G_rGCy'C
Cuts at: 1391 2333 2370 2550 3694 3921 4094 4120 4148
Size: 942 37 180 1144 227 173 26 28
Cuts at: 4148 1391
Size: 4072
Fragments arranged by size:
4072 1144 942 227 180 173 37 28
26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5858
BbvCI CC'TCA_GC
Cuts at: 3041 3041
Size: 6829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3905 3939 5020 5054 1903
Size: 34 1968 34 1081 34 3678
Fragments arranged by size:
3678 1968 1081 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 5931 4721
Size: 1210 5619
BclI T'GATC_A
Cuts at: 1548 2415 1548
Size: 867 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 2249 2337
Size: 107 122 71 631 605 386 191 88
Cuts at: 2337 2471 4108 5440 136
Size: 134 1637 1332 1525
Fragments arranged by size:
1637 1525 1332 631 605 386 191 134
122 107 88 71
BglII A'GATC_T
Cuts at: 3489 6824 3489
Size: 3335 3494
BlpI GC'TnA_GC
Cuts at: 4246 4246
Size: 6829
Bme1580I G_kGCm'C
Cuts at: 1790 2333 3694 4120 4890 6046 1790
Size: 543 1361 426 770 1156 2573
Fragments arranged by size:
2573 1361 1156 770 543 426
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2833 2961 4121
Size: 416 53 83 399 24 1416 128 1160
Cuts at: 4121 4560 5518 442
Size: 439 958 1753
Fragments arranged by size:
1753 1416 1160 958 439 416 399 128
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3511 3511
Size: 6829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2573 3302 3697 5489 1239
Size: 1145 189 729 395 1792 2579
Fragments arranged by size:
2579 1792 1145 729 395 189
Bpu10I CC'TnA_GC
Cuts at: 2184 2544 3041 2184
Size: 360 497 5972
Fragments arranged by size:
5972 497 360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2412 2653 3413 4954 5819 6117 2412
Size: 241 760 1541 865 298 3124
Fragments arranged by size:
3124 1541 865 760 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5492 882
Size: 617 3993 2219
Fragments arranged by size:
3993 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3400 3439 4620 493
Size: 711 2196 39 1181 2702
Fragments arranged by size:
2702 2196 1181 711 39
BsaBI GATnn'nnATC
Cuts at: 6823 6823
Size: 6829
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3366 4636 5018 5653
Size: 53 83 186 936 1833 1270 382 635
Cuts at: 5653 275
Size: 1451
Fragments arranged by size:
1833 1451 1270 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5262 5660 5934 6081 6492 5262
Size: 398 274 147 411 5599
Fragments arranged by size:
5599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3002 3032 1178
Size: 30 165 30 1599 30 4975
Fragments arranged by size:
4975 1599 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 2468 2492 2495 2498 2501 2522 2525
Size: 1123 159 24 3 3 3 21 3
Cuts at: 2525 2528 2906 3023 3080 3083 3164 3167 1186
Size: 3 378 117 57 3 81 3 4848
Fragments arranged by size:
4848 1123 378 159 117 81 57 24
21 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2673 2898 2989 3028 3132 3249 3595 3799 3883
Size: 225 91 39 104 117 346 204 84
Cuts at: 3883 4018 4111 6032 6528 2673
Size: 135 93 1921 496 2974
Fragments arranged by size:
2974 1921 496 346 225 204 135 117
104 93 91 84 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 2670 3314 3968 1163
Size: 573 336 598 644 654 4024
Fragments arranged by size:
4024 654 644 598 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2466 2857 4279 5040 6068 1540
Size: 275 186 465 391 1422 761 1028 2301
Fragments arranged by size:
2301 1422 1028 761 465 391 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 4890 4975 6046 2370
Size: 2520 85 1071 3153
Fragments arranged by size:
3153 2520 1071 85
BsmI GAATG_Cn'
Cuts at: 3863 4340 4433 3863
Size: 477 93 6259
Fragments arranged by size:
6259 477 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2747 2747
Size: 6829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2370 2550 3694 3921 4094 4120
Size: 399 543 37 180 1144 227 173 26
Cuts at: 4120 4148 4890 4975 6046 1391
Size: 28 742 85 1071 2174
Fragments arranged by size:
2174 1144 1071 742 543 399 227 180
173 85 37 28 26
BspEI T'CCGG_A
Cuts at: 6492 6492
Size: 6829
BspHI T'CATG_A
Cuts at: 4718 6448 4718
Size: 1730 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2775 3234 3466 4184 844
Size: 1316 615 459 232 718 3489
Fragments arranged by size:
3489 1316 718 615 459 232
BsrBI CCG'CTC
Cuts at: 1394 1823 4716 1394
Size: 429 2893 3507
Fragments arranged by size:
3507 2893 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2278 5324 5498 65
Size: 1183 1030 3046 174 1396
Fragments arranged by size:
3046 1396 1183 1030 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2770 3878 5473 5660 1700
Size: 110 960 1108 1595 187 2869
Fragments arranged by size:
2869 1595 1108 960 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6829
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 3405 4889 5901 3405
Size: 1484 1012 4333
Fragments arranged by size:
4333 1484 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4028 794
Size: 3234 3595
BstBI TT'CG_AA
Cuts at: 5633 5633
Size: 6829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6829
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 4111 2137
Size: 1974 4855
BstYI r'GATC_y
Cuts at: 1972 3265 3489 4159 4526 4911 4928 6380 6466
Size: 1293 224 670 367 385 17 1452 86
Cuts at: 6466 6478 6824 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1293 670 385 367 346 224
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2800 3006 2800
Size: 206 6623
BtgI C'CryG_G
Cuts at: 513 1065 2053 2163 2247 3083 3837 513
Size: 552 988 110 84 836 754 3505
Fragments arranged by size:
3505 988 836 754 552 110 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3454 487
Size: 683 56 2228 3862
Fragments arranged by size:
3862 2228 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3323 3801 4345 5139 5159 3181
Size: 142 478 544 794 20 4851
Fragments arranged by size:
4851 794 544 478 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6829
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6794
DraI TTT'AAA
Cuts at: 4133 4133
Size: 6829
DraIII CAC_nnn'GTG
Cuts at: 2841 4049 2841
Size: 1208 5621
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5836 809
Size: 507 4520 1802
Fragments arranged by size:
4520 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2463 2854
Size: 54 1425 50 275 18 204 429 391
Cuts at: 2854 3507 3834 4276 5165 6794 8
Size: 653 327 442 889 1629 43
Fragments arranged by size:
1629 1425 889 653 442 429 391 327
275 204 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2463 2854 4276 1537
Size: 275 651 391 1422 4090
Fragments arranged by size:
4090 1422 651 391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 4759 1353
Size: 1146 2260 3423
Fragments arranged by size:
3423 2260 1146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2306 5416 5790 5936 387
Size: 1064 138 717 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 717 374 146 138
EcoICRI GAG'CTC
Cuts at: 2368 2368
Size: 6829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2384 2573 3017 3302 3697 4118 4892
Size: 393 752 189 444 285 395 421 774
Cuts at: 4892 5489 6276 6544 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 774 752 597 444 421 395
393 285 268 189
EcoNI CCTnn'n_nnAGG
Cuts at: 2187 3938 2187
Size: 1751 5078
EcoO109I rG'GnC_Cy
Cuts at: 2060 2141 2149 2192 2330 2405 2566 2577 2805
Size: 81 8 43 138 75 161 11 228
Cuts at: 2805 2977 3690 3691 4116 2060
Size: 172 713 1 425 4773
Fragments arranged by size:
4773 713 425 228 172 161 138 81
75 43 11 8 1
EcoRI G'AATT_C
Cuts at: 3590 4138 4516 3590
Size: 548 378 5903
Fragments arranged by size:
5903 548 378
FspI TGC'GCA
Cuts at: 2156 5335 2156
Size: 3179 3650
HaeII r_GCGC'y
Cuts at: 1678 2163 3602 5976 1678
Size: 485 1439 2374 2531
Fragments arranged by size:
2531 2374 1439 485
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 2651 2683 3224
Size: 32 135 32 935 32 112 32 541
Cuts at: 3224 3256 3724 3756 5470 5502 5544 5576 1373
Size: 32 468 32 1714 32 42 32 2626
Fragments arranged by size:
2626 1714 935 541 468 135 112 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3187 4173 4419 669
Size: 988 281 1083 166 986 246 3079
Fragments arranged by size:
3079 1083 988 986 281 246 166
HindIII A'AGCT_T
Cuts at: 748 3074 748
Size: 2326 4503
HpaI GTT'AAC
Cuts at: 4419 4419
Size: 6829
KpnI G_GTAC'C
Cuts at: 4154 4154
Size: 6829
MfeI C'AATT_G
Cuts at: 3190 4428 3190
Size: 1238 5591
MluI A'CGCG_T
Cuts at: 6705 6705
Size: 6829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 5943 6127 1305
Size: 1482 3156 184 2007
Fragments arranged by size:
3156 2007 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5007
Fragments arranged by size:
5007 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3356 3747 4787 5146 5305 518
Size: 648 969 1221 391 1040 359 159 2042
Fragments arranged by size:
2042 1221 1040 969 648 391 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2135 3280 3528 4245 4922 6070 6315
Size: 501 28 1145 248 717 677 1148 245
Cuts at: 6315 1606
Size: 2120
Fragments arranged by size:
2120 1148 1145 717 677 501 248 245
28
NaeI GCC'GGC
Cuts at: 2772 3880 2772
Size: 1108 5721
NcoI C'CATG_G
Cuts at: 513 1065 2163 513
Size: 552 1098 5179
Fragments arranged by size:
5179 1098 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6829
NgoMIV G'CCGG_C
Cuts at: 2770 3878 2770
Size: 1108 5721
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6829
NotI GC'GGCC_GC
Cuts at: 4276 4276
Size: 6829
NspI r_CATG'y
Cuts at: 1132 1229 2641 3907 3964 4187 5732 1132
Size: 97 1412 1266 57 223 1545 2229
Fragments arranged by size:
2229 1545 1412 1266 223 97 57
PasI CC'CwG_GG
Cuts at: 1348 2534 2689 3098 1348
Size: 1186 155 409 5079
Fragments arranged by size:
5079 1186 409 155
PciI A'CATG_T
Cuts at: 3903 5728 3903
Size: 1825 5004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2841 1108
Size: 1733 5096
PfoI T'CCnGG_A
Cuts at: 2790 2790
Size: 6829
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PmlI CAC'GTG
Cuts at: 3439 3439
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 6411 6443 4898
Size: 32 1481 32 5284
Fragments arranged by size:
5284 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2149 2405 2805 2141
Size: 8 256 400 6165
Fragments arranged by size:
6165 400 256 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6829
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 6829
PspOMI G'GGCC_C
Cuts at: 2329 3690 4116 2329
Size: 1361 426 5042
Fragments arranged by size:
5042 1361 426
PspXI vC'TCGA_Gb
Cuts at: 3695 3695
Size: 6829
PstI C_TGCA'G
Cuts at: 830 2127 3279 4181 830
Size: 1297 1152 902 3478
Fragments arranged by size:
3478 1297 1152 902
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
PvuII CAG'CTG
Cuts at: 2107 2135 3280 2107
Size: 28 1145 5656
Fragments arranged by size:
5656 1145 28
SacI G_AGCT'C
Cuts at: 2370 2370
Size: 6829
SalI G'TCGA_C
Cuts at: 1936 4171 1936
Size: 2235 4594
SanDI GG'GwC_CC
Cuts at: 2141 2141
Size: 6829
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 5077 6789 5077
Size: 1712 5117
SfcI C'TryA_G
Cuts at: 826 1045 2123 3275 4177 5312 5993 6184 826
Size: 219 1078 1152 902 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 1078 902 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2249 2337 2471 2249
Size: 88 134 6607
Fragments arranged by size:
6607 134 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 2603 4156 1872
Size: 731 1553 4545
Fragments arranged by size:
4545 1553 731
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2427 2668 3428 3695 4933 5834
Size: 197 926 484 241 760 267 1238 901
Cuts at: 5834 6096 820
Size: 262 1553
Fragments arranged by size:
1553 1238 926 901 760 484 267 262
241 197
SnaBI TAC'GTA
Cuts at: 493 3400 4620 493
Size: 2907 1220 2702
Fragments arranged by size:
2907 2702 1220
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6829
SphI G_CATG'C
Cuts at: 1132 2641 3964 4187 1132
Size: 1509 1323 223 3774
Fragments arranged by size:
3774 1509 1323 223
SspI AAT'ATT
Cuts at: 5 52 4753 5
Size: 47 4701 2081
Fragments arranged by size:
4701 2081 47
StuI AGG'CCT
Cuts at: 1517 2340 2397 4001 1517
Size: 823 57 1604 4345
Fragments arranged by size:
4345 1604 823 57
StyI C'CwwG_G
Cuts at: 513 1065 2145 2163 2286 2325 3127 4268 513
Size: 552 1080 18 123 39 802 1141 3074
Fragments arranged by size:
3074 1141 1080 802 552 123 39 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2852 3161 4856 4873 5026 5211 5656
Size: 1255 1272 309 1695 17 153 185 445
Cuts at: 5656 325
Size: 1498
Fragments arranged by size:
1695 1498 1272 1255 445 309 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 5075 6787
Size: 274 80 33 51 1443 375 2723 1712
Cuts at: 6787 96
Size: 138
Fragments arranged by size:
2723 1712 1443 375 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6789 6821 6789
Size: 32 6797
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6829
XbaI T'CTAG_A
Cuts at: 4165 4165
Size: 6829
XhoI C'TCGA_G
Cuts at: 1943 3695 1943
Size: 1752 5077
XmaI C'CCGG_G
Cuts at: 1870 2601 4154 1870
Size: 731 1553 4545
Fragments arranged by size:
4545 1553 731
XmnI GAAnn'nnTTC
Cuts at: 4958 4958
Size: 6829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BsiWI BstZ17I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI
XcmI