Restriction map for
pFN21AB8011
(Circular) (Six-base) MAPSORT of: pFN21AB8011.seq Check: 3984 from: 1 to: 5947
pFN21AB8011 5947 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2607 2818 4010 5394 5662 1632
Size: 975 211 1192 1384 268 1917
Fragments arranged by size:
1917 1384 1192 975 268 211
AfeI AGC'GCT
Cuts at: 2013 2013
Size: 5947
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2452 4846 5823 1203
Size: 1249 2394 977 1327
Fragments arranged by size:
2394 1327 1249 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 3080 1166
Size: 1914 4033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2598 1184
Size: 1414 4533
AlwNI CAG_nnn'CTG
Cuts at: 2759 3690 5262 2759
Size: 931 1572 3444
Fragments arranged by size:
3444 1572 931
ApaLI G'TGCA_C
Cuts at: 2448 4004 5160 2448
Size: 1556 1156 3235
Fragments arranged by size:
3235 1556 1156
ApoI r'AATT_y
Cuts at: 1493 2349 3256 3482 3634 5743 1493
Size: 856 907 226 152 2109 1697
Fragments arranged by size:
2109 1697 907 856 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 4723 618
Size: 325 217 627 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3266 1391
Size: 1875 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2132 928
Size: 971 233 4743
Fragments arranged by size:
4743 971 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2947 2981 4138 4172 1903
Size: 34 1010 34 1157 34 3678
Fragments arranged by size:
3678 1157 1010 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3243 4558 136
Size: 107 122 71 631 605 1571 1315 1525
Fragments arranged by size:
1571 1525 1315 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 2752 3203 3364 2752
Size: 451 161 5335
Fragments arranged by size:
5335 451 161
Bme1580I G_kGCm'C
Cuts at: 1790 2452 2712 4008 5164 1790
Size: 662 260 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 662 260
BmgBI CAC'GTC
Cuts at: 1318 2955 1318
Size: 1637 4310
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2415 2677 2699 2717 3128 4607 1239
Size: 1100 76 262 22 18 411 1479 2579
Fragments arranged by size:
2579 1479 1100 411 262 76 22 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2675 2782 4610 882
Size: 617 1176 107 1828 2219
Fragments arranged by size:
2219 1828 1176 617 107
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 2292 5941 2292
Size: 3649 2298
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2135 2720 2770 1186
Size: 901 48 585 50 4363
Fragments arranged by size:
4363 901 585 50 48
BseYI C'CCAG_C
Cuts at: 2067 2094 5150 5646 2067
Size: 27 3056 496 2368
Fragments arranged by size:
3056 2368 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 2909 1163
Size: 573 858 315 4201
Fragments arranged by size:
4201 858 573 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2452 4008 4093 5164 2452
Size: 1556 85 1071 3235
Fragments arranged by size:
3235 1556 1071 85
BsmI GAATG_Cn'
Cuts at: 2591 3458 3551 2591
Size: 867 93 4987
Fragments arranged by size:
4987 867 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3173 3173
Size: 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2452 2712 3266 4008 4093 5164 1391
Size: 399 662 260 554 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 662 554 399 260 85
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2751 3834 1394
Size: 429 928 1083 3507
Fragments arranged by size:
3507 1083 928 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 3015 96
Size: 2919 3028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2645 794
Size: 1851 4096
BstBI TT'CG_AA
Cuts at: 2418 4751 2418
Size: 2333 3614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2190 2260 2344 2190
Size: 70 84 5793
Fragments arranged by size:
5793 84 70
BstYI r'GATC_y
Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942
Size: 1305 367 385 17 1452 86 12 346
Cuts at: 5942 1972
Size: 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2343 2443 513
Size: 552 939 339 100 4017
Fragments arranged by size:
4017 939 552 339 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2885 2984 487
Size: 683 56 1659 99 3450
Fragments arranged by size:
3450 1659 683 99 56
BtsI GCAGTG_nn'
Cuts at: 2337 2502 2970 3020 3463 4257 4277 2337
Size: 165 468 50 443 794 20 4007
Fragments arranged by size:
4007 794 468 443 165 50 20
ClaI AT'CG_AT
Cuts at: 1554 2794 1554
Size: 1240 4707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912
Size: 54 1425 50 275 18 1564 889 1629
Cuts at: 5912 8
Size: 43
Fragments arranged by size:
1629 1564 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2619 3171 3877 1353
Size: 1266 552 706 3423
Fragments arranged by size:
3423 1266 706 552
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2415 2607 2677 2699 2717 2818
Size: 393 707 76 192 70 22 18 101
Cuts at: 2818 3128 4010 4607 5394 5662 1239
Size: 310 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 707 597 393 310 268
192 101 76 70 22 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3037 3037
Size: 5947
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3245 3245
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 2015 3203 5094 1678
Size: 337 1188 1891 2531
Fragments arranged by size:
2531 1891 1188 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4588 4620 4662 4694 1373
Size: 32 135 32 3016 32 42 32 2626
Fragments arranged by size:
3016 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 3291 3537 669
Size: 988 281 295 1058 246 3079
Fragments arranged by size:
3079 1058 988 295 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5947
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2321 2873 2940 3015 5061 5245 1305
Size: 1016 552 67 75 2046 184 2007
Fragments arranged by size:
2046 2007 1016 552 184 75 67
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2188 2342 3080 3905 4264 4423 518
Size: 648 1022 154 738 825 359 159 2042
Fragments arranged by size:
2042 1022 825 738 648 359 159 154
MspA1I CmG'CkG
Cuts at: 1606 3006 3147 3363 4040 5188 5433 1606
Size: 1400 141 216 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 245 216 141
NcoI C'CATG_G
Cuts at: 513 1065 2004 2343 2443 513
Size: 552 939 339 100 4017
Fragments arranged by size:
4017 939 552 339 100
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2456 3154 3305 4850 1132
Size: 97 1227 698 151 1545 2229
Fragments arranged by size:
2229 1545 1227 698 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 2452 4846 2452
Size: 2394 3553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2150 1108
Size: 1042 4905
PfoI T'CCnGG_A
Cuts at: 2733 2971 2733
Size: 238 5709
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2835 1613
Size: 1222 4725
PsiI TTA'TAA
Cuts at: 2883 3517 2883
Size: 634 5313
PstI C_TGCA'G
Cuts at: 830 2471 3299 830
Size: 1641 828 3478
Fragments arranged by size:
3478 1641 828
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
PvuII CAG'CTG
Cuts at: 3006 3006
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 2471 3299 2471
Size: 828 5119
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2467 3295 4430 5111 5302 826
Size: 219 1422 828 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 828 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3154 3305 1132
Size: 2022 151 3774
Fragments arranged by size:
3774 2022 151
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 3043 1517
Size: 1526 4421
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2343 2443 2529 2817 3033
Size: 552 939 81 258 100 86 288 216
Cuts at: 3033 3386 513
Size: 353 3074
Fragments arranged by size:
3074 939 552 353 288 258 216 100
86 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3001 3974 3991 4144 4329 4774 325
Size: 1255 1421 973 17 153 185 445 1498
Fragments arranged by size:
1498 1421 1255 973 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2985 3015 3084
Size: 274 80 33 51 1443 1008 30 69
Cuts at: 3084 3126 4193 5905 96
Size: 42 1067 1712 138
Fragments arranged by size:
1712 1443 1067 1008 274 138 80 69
51 42 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2729 2761 5907 5939 2729
Size: 32 3146 32 2737
Fragments arranged by size:
3146 2737 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2674 3227 2674
Size: 553 5394
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI