Restriction map for
pFN21AB8006
(Circular) (Six-base) MAPSORT of: pFN21AB8006.seq Check: 3070 from: 1 to: 7024
pFN21AB8006 7024 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2320 3259 2320
Size: 939 6085
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4834 278
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3828 4367 1937
Size: 1891 539 4594
Fragments arranged by size:
4594 1891 539
Acc65I G'GTAC_C
Cuts at: 4345 4345
Size: 7024
AclI AA'CG_TT
Cuts at: 1563 5151 5524 1563
Size: 3588 373 3063
Fragments arranged by size:
3588 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3780 4031 5087 6471 6739 1632
Size: 2148 251 1056 1384 268 1917
Fragments arranged by size:
2148 1917 1384 1056 268 251
AfeI AGC'GCT
Cuts at: 2091 2091
Size: 7024
AflII C'TTAA_G
Cuts at: 820 1017 2339 2348 820
Size: 197 1322 9 5496
Fragments arranged by size:
5496 1322 197 9
AflIII A'CryG_T
Cuts at: 1203 3912 4063 5923 6900 1203
Size: 2709 151 1860 977 1327
Fragments arranged by size:
2709 1860 1327 977 151
AgeI A'CCGG_T
Cuts at: 5855 5855
Size: 7024
AhdI GACnn_n'nnGTC
Cuts at: 2824 5753 2824
Size: 2929 4095
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2328 4182 1184
Size: 1144 1854 4026
Fragments arranged by size:
4026 1854 1144
AlwNI CAG_nnn'CTG
Cuts at: 3254 4054 4767 6339 3254
Size: 800 713 1572 3939
Fragments arranged by size:
3939 1572 800 713
ApaI G_GGCC'C
Cuts at: 2046 3216 3262 2046
Size: 1170 46 5808
Fragments arranged by size:
5808 1170 46
ApaLI G'TGCA_C
Cuts at: 4309 5081 6237 4309
Size: 772 1156 5096
Fragments arranged by size:
5096 1156 772
ApoI r'AATT_y
Cuts at: 1493 2710 3320 3374 4333 4559 4711 6820 1493
Size: 1217 610 54 959 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 959 610 226 152 54
AseI AT'TA_AT
Cuts at: 160 1032 5578 160
Size: 872 4546 1606
Fragments arranged by size:
4546 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
AvaI C'yCGr_G
Cuts at: 1870 1943 2976 3208 3314 3711 3788 4349 1870
Size: 73 1033 232 106 397 77 561 4545
Fragments arranged by size:
4545 1033 561 397 232 106 77 73
BamHI G'GATC_C
Cuts at: 2237 4354 4721 2237
Size: 2117 367 4540
Fragments arranged by size:
4540 2117 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3632 4345 5800 618
Size: 325 217 627 1845 713 1455 1842
Fragments arranged by size:
1845 1842 1455 713 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2046 2077 3216 3262 4305 4343 1391
Size: 633 22 31 1139 46 1043 38 4072
Fragments arranged by size:
4072 1139 1043 633 46 38 31 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6053
BbvCI CC'TCA_GC
Cuts at: 2193 3243 4199 4233 2193
Size: 1050 956 34 4984
Fragments arranged by size:
4984 1050 956 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2171 2205 2729 2763 3389 3423 5215
Size: 34 234 34 524 34 626 34 1792
Cuts at: 5215 5249 1903
Size: 34 3678
Fragments arranged by size:
3678 1792 626 524 234 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4916 6126 4916
Size: 1210 5814
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7024
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3257 5635 136
Size: 107 122 71 631 605 1585 2378 1525
Fragments arranged by size:
2378 1585 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7019 7019
Size: 7024
BlpI GC'TnA_GC
Cuts at: 3390 4441 3390
Size: 1051 5973
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2776 3216 3262 4313 5085 6241 1790
Size: 256 730 440 46 1051 772 1156 2573
Fragments arranged by size:
2573 1156 1051 772 730 440 256 46
BmgBI CAC'GTC
Cuts at: 1318 2434 2479 3915 4321 1318
Size: 1116 45 1436 406 4021
Fragments arranged by size:
4021 1436 1116 406 45
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4084 4755 5713
Size: 416 53 83 399 24 2667 671 958
Cuts at: 5713 442
Size: 1753
Fragments arranged by size:
2667 1753 958 671 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4232 1056
Size: 3176 3848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2043 2091 3208 2041
Size: 2 48 1117 5857
Fragments arranged by size:
5857 1117 48 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2967 3188 3356 5684 1239
Size: 1728 221 168 2328 2579
Fragments arranged by size:
2579 2328 1728 221 168
Bpu10I CC'TnA_GC
Cuts at: 2193 2459 3149 3243 4199 4233 2193
Size: 266 690 94 956 34 4984
Fragments arranged by size:
4984 956 690 266 94 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3560 5149 6014 6312 3560
Size: 1589 865 298 4272
Fragments arranged by size:
4272 1589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5687 882
Size: 617 4188 2219
Fragments arranged by size:
4188 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4815 493
Size: 711 3611 2702
Fragments arranged by size:
3611 2702 711
BsaBI GATnn'nnATC
Cuts at: 7018 7018
Size: 7024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4280 4831 5213 5848
Size: 53 83 186 936 2747 551 382 635
Cuts at: 5848 275
Size: 1451
Fragments arranged by size:
2747 1451 936 635 551 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2319 5457 5855 6129 6276 6687 2319
Size: 3138 398 274 147 411 2656
Fragments arranged by size:
3138 2656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 2494 2524 2940
Size: 30 165 30 640 30 421 30 416
Cuts at: 2940 2970 1178
Size: 30 5232
Fragments arranged by size:
5232 640 421 416 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2570 3030 4229 4259 1186
Size: 1063 321 460 1199 30 3951
Fragments arranged by size:
3951 1199 1063 460 321 30
BseYI C'CCAG_C
Cuts at: 3848 4287 6227 6723 3848
Size: 439 1940 496 4149
Fragments arranged by size:
4149 1940 496 439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2160 2867 3536 4040 4175 1163
Size: 573 424 707 669 504 135 4012
Fragments arranged by size:
4012 707 669 573 504 424 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4474 5235 6263 1540
Size: 275 186 2473 761 1028 2301
Fragments arranged by size:
2473 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2077 2601 2880 4313 5085 5170 6241 2077
Size: 524 279 1433 772 85 1071 2860
Fragments arranged by size:
2860 1433 1071 772 524 279 85
BsmI GAATG_Cn'
Cuts at: 4535 4628 4535
Size: 93 6931
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2046 2077 2601 2776 2880 3216
Size: 399 234 22 31 524 175 104 336
Cuts at: 3216 3262 4305 4313 4343 5085 5170 6241 1391
Size: 46 1043 8 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 1043 742 524 399 336 234
175 104 85 46 31 30 22 8
BspEI T'CCGG_A
Cuts at: 2319 6687 2319
Size: 4368 2656
BspHI T'CATG_A
Cuts at: 2928 4913 6643 2928
Size: 1985 1730 3309
Fragments arranged by size:
3309 1985 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 3259 4197 4379 844
Size: 1476 939 938 182 3489
Fragments arranged by size:
3489 1476 939 938 182
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 3394 4911 1394
Size: 429 209 1362 1517 3507
Fragments arranged by size:
3507 1517 1362 429 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 5519 5693 65
Size: 1183 4271 174 1396
Fragments arranged by size:
4271 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5668 5855 1700
Size: 110 3858 187 2869
Fragments arranged by size:
3858 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2412 96
Size: 2316 4708
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7024
BssSI C'ACGA_G
Cuts at: 3554 3878 5084 6096 3554
Size: 324 1206 1012 4482
Fragments arranged by size:
4482 1206 1012 324
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3988 794
Size: 3194 3830
BstBI TT'CG_AA
Cuts at: 3325 5828 3325
Size: 2503 4521
BstEII G'GTnAC_C
Cuts at: 1184 2983 3640 1184
Size: 1799 657 4568
Fragments arranged by size:
4568 1799 657
BstXI CCAn_nnnn'nTGG
Cuts at: 2945 2945
Size: 7024
BstYI r'GATC_y
Cuts at: 1972 2237 3665 3870 4041 4354 4721 5106 5123
Size: 265 1428 205 171 313 367 385 17
Cuts at: 5123 6575 6661 6673 7019 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1428 385 367 346 313 265
205 171 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3898 4314 513
Size: 552 939 1894 416 3223
Fragments arranged by size:
3223 1894 939 552 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6285
Fragments arranged by size:
6285 683 56
BtsI GCAGTG_nn'
Cuts at: 2601 3112 4540 5334 5354 2601
Size: 511 1428 794 20 4271
Fragments arranged by size:
4271 1428 794 511 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2869 2904 529
Size: 35 2305 35 4649
Fragments arranged by size:
4649 2305 35 35
DraI TTT'AAA
Cuts at: 4328 4328
Size: 7024
DraIII CAC_nnn'GTG
Cuts at: 3988 3988
Size: 7024
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3824 6031 809
Size: 507 2508 2207 1802
Fragments arranged by size:
2508 2207 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2281 2890 4099
Size: 54 1425 50 275 18 451 609 1209
Cuts at: 4099 4471 5360 6989 8
Size: 372 889 1629 43
Fragments arranged by size:
1629 1425 1209 889 609 451 372 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4471 1537
Size: 275 2659 4090
Fragments arranged by size:
4090 2659 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 2694 3756 3947 4217 4954 1353
Size: 876 465 1062 191 270 737 3423
Fragments arranged by size:
3423 1062 876 737 465 270 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 2823 3446 3572 4273 5611
Size: 1064 138 867 367 623 126 701 1338
Cuts at: 5611 5985 6131 387
Size: 374 146 1280
Fragments arranged by size:
1338 1280 1064 867 701 623 374 367
146 138 126
EcoICRI GAG'CTC
Cuts at: 2075 2075
Size: 7024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2967 3188 3356 3780 4031 5087 5684
Size: 393 1335 221 168 424 251 1056 597
Cuts at: 5684 6471 6739 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1335 1056 787 597 424 393 268
251 221 168
EcoNI CCTnn'n_nnAGG
Cuts at: 2992 3266 2992
Size: 274 6750
EcoO109I rG'GnC_Cy
Cuts at: 2197 3212 3213 3258 3259 2197
Size: 1015 1 45 1 5962
Fragments arranged by size:
5962 1015 45 1 1
EcoRI G'AATT_C
Cuts at: 4333 4711 4333
Size: 378 6646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2783 3642 2783
Size: 859 6165
FspI TGC'GCA
Cuts at: 4091 5530 4091
Size: 1439 5585
HaeII r_GCGC'y
Cuts at: 1678 2093 6171 1678
Size: 415 4078 2531
Fragments arranged by size:
4078 2531 415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2162 2176 2194 2810
Size: 32 135 32 572 18 14 18 616
Cuts at: 2810 2842 5665 5697 5739 5771 1373
Size: 32 2823 32 42 32 2626
Fragments arranged by size:
2823 2626 616 572 135 42 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4368 4614 669
Size: 988 281 2430 246 3079
Fragments arranged by size:
3079 2430 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7024
HpaI GTT'AAC
Cuts at: 4614 4614
Size: 7024
KpnI G_GTAC'C
Cuts at: 4349 4349
Size: 7024
MfeI C'AATT_G
Cuts at: 4623 4623
Size: 7024
MluI A'CGCG_T
Cuts at: 6900 6900
Size: 7024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3997 6138 6322 1305
Size: 2692 2141 184 2007
Fragments arranged by size:
2692 2141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2283 10
Size: 54 1425 343 451 4751
Fragments arranged by size:
4751 1425 451 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4062 4982 5341 5500 518
Size: 648 2896 920 359 159 2042
Fragments arranged by size:
2896 2042 920 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2911 4051 4440 5117 6265 6510 1606
Size: 1305 1140 389 677 1148 245 2120
Fragments arranged by size:
2120 1305 1148 1140 677 389 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3898 513
Size: 552 939 1894 3639
Fragments arranged by size:
3639 1894 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7024
NheI G'CTAG_C
Cuts at: 1052 4228 1052
Size: 3176 3848
NotI GC'GGCC_GC
Cuts at: 4471 4471
Size: 7024
NspI r_CATG'y
Cuts at: 1132 1229 2486 3565 4067 4096 4382 5927 1132
Size: 97 1257 1079 502 29 286 1545 2229
Fragments arranged by size:
2229 1545 1257 1079 502 286 97 29
PasI CC'CwG_GG
Cuts at: 1348 3254 3262 1348
Size: 1906 8 5110
Fragments arranged by size:
5110 1906 8
PciI A'CATG_T
Cuts at: 4063 5923 4063
Size: 1860 5164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3904 1108
Size: 2796 4228
PmeI GTTT'AAAC
Cuts at: 4328 4328
Size: 7024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 6606 6638 5093
Size: 32 1481 32 5479
Fragments arranged by size:
5479 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2197
Size: 7024
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7024
PsiI TTA'TAA
Cuts at: 3069 4594 3069
Size: 1525 5499
PspOMI G'GGCC_C
Cuts at: 2042 3212 3258 2042
Size: 1170 46 5808
Fragments arranged by size:
5808 1170 46
PspXI vC'TCGA_Gb
Cuts at: 3788 3788
Size: 7024
PstI C_TGCA'G
Cuts at: 830 2390 3163 4161 4376 830
Size: 1560 773 998 215 3478
Fragments arranged by size:
3478 1560 998 773 215
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7024
PvuII CAG'CTG
Cuts at: 4051 4051
Size: 7024
RsrII CG'GwC_CG
Cuts at: 2317 2317
Size: 7024
SacI G_AGCT'C
Cuts at: 2077 2077
Size: 7024
SalI G'TCGA_C
Cuts at: 1936 4366 1936
Size: 2430 4594
SbfI CC_TGCA'GG
Cuts at: 4376 4376
Size: 7024
ScaI AGT'ACT
Cuts at: 5272 6984 5272
Size: 1712 5312
SexAI A'CCwGG_T
Cuts at: 2987 2987
Size: 7024
SfcI C'TryA_G
Cuts at: 826 1045 2275 2386 2901 3159 4157 4372 5507
Size: 219 1230 111 515 258 998 215 1135
Cuts at: 5507 6188 6379 826
Size: 681 191 1471
Fragments arranged by size:
1471 1230 1135 998 681 515 258 219
215 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7024
SmaI CCC'GGG
Cuts at: 1872 3210 4351 1872
Size: 1338 1141 4545
Fragments arranged by size:
4545 1338 1141
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 2348 3314 3575 3788 5128
Size: 197 926 396 9 966 261 213 1340
Cuts at: 5128 6029 6291 820
Size: 901 262 1553
Fragments arranged by size:
1553 1340 966 926 901 396 262 261
213 197 9
SnaBI TAC'GTA
Cuts at: 493 4815 493
Size: 4322 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7024
SphI G_CATG'C
Cuts at: 1132 2486 4382 1132
Size: 1354 1896 3774
Fragments arranged by size:
3774 1896 1354
SspI AAT'ATT
Cuts at: 5 52 4948 5
Size: 47 4896 2081
Fragments arranged by size:
4896 2081 47
StuI AGG'CCT
Cuts at: 1517 2705 2766 1517
Size: 1188 61 5775
Fragments arranged by size:
5775 1188 61
StyI C'CwwG_G
Cuts at: 513 1065 2004 3898 4463 513
Size: 552 939 1894 565 3074
Fragments arranged by size:
3074 1894 939 565 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3188 3511 3896 4074 5051 5068 5221
Size: 1255 1608 323 385 178 977 17 153
Cuts at: 5221 5406 5851 325
Size: 185 445 1498
Fragments arranged by size:
1608 1498 1255 977 445 385 323 185
178 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2412 3558 5270
Size: 274 80 33 51 1443 435 1146 1712
Cuts at: 5270 6982 96
Size: 1712 138
Fragments arranged by size:
1712 1712 1443 1146 435 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2043 2075 2301 2333 2494 2526 2940 2972 3711
Size: 32 226 32 161 32 414 32 739
Cuts at: 3711 3743 4026 4058 6984 7016 2043
Size: 32 283 32 2926 32 2051
Fragments arranged by size:
2926 2051 739 414 283 226 161 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7024
XbaI T'CTAG_A
Cuts at: 4360 4360
Size: 7024
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2828 2828
Size: 7024
XhoI C'TCGA_G
Cuts at: 1943 3314 3788 1943
Size: 1371 474 5179
Fragments arranged by size:
5179 1371 474
XmaI C'CCGG_G
Cuts at: 1870 3208 4349 1870
Size: 1338 1141 4545
Fragments arranged by size:
4545 1338 1141
XmnI GAAnn'nnTTC
Cuts at: 3374 3933 5153 3374
Size: 559 1220 5245
Fragments arranged by size:
5245 1220 559
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4832 276
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PsrI SacII
SanDI SapI SfiI SfoI SrfI SwaI