Restriction map for
pFN21AB8004
(Circular) (Six-base) MAPSORT of: pFN21AB8004.seq Check: 9225 from: 1 to: 6817
pFN21AB8004 6817 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4627 278
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2526 2733 4160 1937
Size: 589 207 1427 4594
Fragments arranged by size:
4594 1427 589 207
Acc65I G'GTAC_C
Cuts at: 2066 3426 4138 2066
Size: 1360 712 4745
Fragments arranged by size:
4745 1360 712
AclI AA'CG_TT
Cuts at: 1563 4944 5317 1563
Size: 3381 373 3063
Fragments arranged by size:
3381 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2483 2505 2562 2654 3319 4880 6264 6532
Size: 851 22 57 92 665 1561 1384 268
Cuts at: 6532 1632
Size: 1917
Fragments arranged by size:
1917 1561 1384 851 665 268 92 57
22
AfeI AGC'GCT
Cuts at: 3789 3789
Size: 6817
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6620
AflIII A'CryG_T
Cuts at: 1203 5716 6693 1203
Size: 4513 977 1327
Fragments arranged by size:
4513 1327 977
AgeI A'CCGG_T
Cuts at: 5648 5648
Size: 6817
AhdI GACnn_n'nnGTC
Cuts at: 5546 5546
Size: 6817
AleI CACnn'nnGTG
Cuts at: 1166 3923 1166
Size: 2757 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6817
AlwNI CAG_nnn'CTG
Cuts at: 2215 2766 3991 4560 6132 2215
Size: 551 1225 569 1572 2900
Fragments arranged by size:
2900 1572 1225 569 551
ApaI G_GGCC'C
Cuts at: 3621 3621
Size: 6817
ApaLI G'TGCA_C
Cuts at: 4874 6030 4874
Size: 1156 5661
ApoI r'AATT_y
Cuts at: 1493 4126 4352 4504 6613 1493
Size: 2633 226 152 2109 1697
Fragments arranged by size:
2633 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5371 160
Size: 872 4339 1606
Fragments arranged by size:
4339 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 3402 4075 4142 1870
Size: 73 88 1371 673 67 4545
Fragments arranged by size:
4545 1371 673 88 73 67
BamHI G'GATC_C
Cuts at: 3126 4147 4514 3126
Size: 1021 367 5429
Fragments arranged by size:
5429 1021 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2425 3426 3964 4138
Size: 325 217 627 279 359 1001 538 174
Cuts at: 4138 5593 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1001 627 538 359 325 279
217 174
BanII G_rGCy'C
Cuts at: 1391 3201 3621 3684 3728 3738 4136 1391
Size: 1810 420 63 44 10 398 4072
Fragments arranged by size:
4072 1810 420 398 63 44 10
BbeI G_GCGC'C
Cuts at: 3968 3968
Size: 6817
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2961 3407 928
Size: 971 1062 446 4338
Fragments arranged by size:
4338 1062 971 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2266 2300 2633 2667 5008 5042 1903
Size: 34 329 34 333 34 2341 34 3678
Fragments arranged by size:
3678 2341 333 329 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2453 4709 5919 2453
Size: 2256 1210 3351
Fragments arranged by size:
3351 2256 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6817
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3962 3979 5428
Size: 107 122 71 631 605 2290 17 1449
Cuts at: 5428 136
Size: 1525
Fragments arranged by size:
2290 1525 1449 631 605 122 107 71
17
BglII A'GATC_T
Cuts at: 3093 6812 3093
Size: 3719 3098
BlpI GC'TnA_GC
Cuts at: 4234 4234
Size: 6817
Bme1580I G_kGCm'C
Cuts at: 1790 2430 3621 4878 6034 1790
Size: 640 1191 1257 1156 2573
Fragments arranged by size:
2573 1257 1191 1156 640
BmgBI CAC'GTC
Cuts at: 1318 3049 1318
Size: 1731 5086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2396 2433 2539
Size: 416 53 83 399 24 979 37 106
Cuts at: 2539 3215 4548 5506 442
Size: 676 1333 958 1753
Fragments arranged by size:
1753 1333 979 958 676 416 399 106
83 53 37 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 2738 3038 3131 5477 1239
Size: 943 556 300 93 2346 2579
Fragments arranged by size:
2579 2346 943 556 300 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 3137 3740 4942 5807 6105 2281
Size: 856 603 1202 865 298 2993
Fragments arranged by size:
2993 1202 865 856 603 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3703 5480 882
Size: 617 2204 1777 2219
Fragments arranged by size:
2219 2204 1777 617
BsaAI yAC'GTr
Cuts at: 493 1204 4608 493
Size: 711 3404 2702
Fragments arranged by size:
3404 2702 711
BsaBI GATnn'nnATC
Cuts at: 2396 6811 2396
Size: 4415 2402
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3522 3965 3971 4624
Size: 53 83 186 936 1989 443 6 653
Cuts at: 4624 5006 5641 275
Size: 382 635 1451
Fragments arranged by size:
1989 1451 936 653 635 443 382 186
83 53 6
BsaWI w'CCGG_w
Cuts at: 3294 3730 5250 5648 5922 6069 6480 3294
Size: 436 1520 398 274 147 411 3631
Fragments arranged by size:
3631 1520 436 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2975 3005 3022
Size: 30 165 30 624 30 918 30 17
Cuts at: 3022 3052 3089 3119 3297 3327 3593 3623 3725
Size: 30 37 30 178 30 266 30 102
Cuts at: 3725 3755 1178
Size: 30 4240
Fragments arranged by size:
4240 918 624 266 178 165 102 37
30 30 30 30 30 30 30 30
30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 3188 3692 4042 1186
Size: 958 1044 504 350 3961
Fragments arranged by size:
3961 1044 958 504 350
BseYI C'CCAG_C
Cuts at: 2148 2233 2429 2759 3254 3870 6020 6516 2148
Size: 85 196 330 495 616 2150 496 2449
Fragments arranged by size:
2449 2150 616 496 495 330 196 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3225 1163
Size: 573 1489 4755
Fragments arranged by size:
4755 1489 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3057 4267 5028 6056 1540
Size: 275 186 1056 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1056 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3684 3728 3930 4878 4963 6034 3684
Size: 44 202 948 85 1071 4467
Fragments arranged by size:
4467 1071 948 202 85 44
BsmI GAATG_Cn'
Cuts at: 2278 4328 4421 2278
Size: 2050 93 4674
Fragments arranged by size:
4674 2050 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3055 3055
Size: 6817
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 3201 3621 3684 3728 3738 3930
Size: 399 640 771 420 63 44 10 192
Cuts at: 3930 4136 4878 4963 6034 1391
Size: 206 742 85 1071 2174
Fragments arranged by size:
2174 1071 771 742 640 420 399 206
192 85 63 44 10
BspEI T'CCGG_A
Cuts at: 6480 6480
Size: 6817
BspHI T'CATG_A
Cuts at: 2805 4706 6436 2805
Size: 1901 1730 3186
Fragments arranged by size:
3186 1901 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4172 844
Size: 3328 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2619 4704 1394
Size: 429 796 2085 3507
Fragments arranged by size:
3507 2085 796 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2377 5312 5486 65
Size: 1183 1129 2935 174 1396
Fragments arranged by size:
2935 1396 1183 1129 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4005 5461 5648 1700
Size: 110 2195 1456 187 2869
Fragments arranged by size:
2869 2195 1456 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6817
BssSI C'ACGA_G
Cuts at: 4877 5889 4877
Size: 1012 5805
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6817
BstBI TT'CG_AA
Cuts at: 5621 5621
Size: 6817
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6817
BstXI CCAn_nnnn'nTGG
Cuts at: 4031 4031
Size: 6817
BstYI r'GATC_y
Cuts at: 1972 2741 3093 3126 4147 4514 4899 4916 6368
Size: 769 352 33 1021 367 385 17 1452
Cuts at: 6368 6454 6466 6812 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1021 769 385 367 352 346
86 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 3189 3189
Size: 6817
BtgI C'CryG_G
Cuts at: 513 1065 2004 2690 3072 3613 3693 513
Size: 552 939 686 382 541 80 3637
Fragments arranged by size:
3637 939 686 552 541 382 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6078
Fragments arranged by size:
6078 683 56
BtsI GCAGTG_nn'
Cuts at: 2282 3868 4333 5127 5147 2282
Size: 1586 465 794 20 3952
Fragments arranged by size:
3952 1586 794 465 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2286 2321 529
Size: 35 1722 35 5025
Fragments arranged by size:
5025 1722 35 35
DraI TTT'AAA
Cuts at: 2872 4121 2872
Size: 1249 5568
DraIII CAC_nnn'GTG
Cuts at: 2248 3644 2248
Size: 1396 5421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5824 809
Size: 507 4508 1802
Fragments arranged by size:
4508 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3054 4264
Size: 54 1425 50 275 18 177 1047 1210
Cuts at: 4264 5153 6782 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1210 1047 889 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3054 4264 1537
Size: 275 1242 1210 4090
Fragments arranged by size:
4090 1242 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 3935 4747 1353
Size: 996 1586 812 3423
Fragments arranged by size:
3423 1586 996 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 3554 5404 5778 5924 387
Size: 1064 138 507 1458 1850 374 146 1280
Fragments arranged by size:
1850 1458 1280 1064 507 374 146 138
EcoICRI GAG'CTC
Cuts at: 3682 3726 3682
Size: 44 6773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2483 2505 2562 2654 2738 3038
Size: 393 550 301 22 57 92 84 300
Cuts at: 3038 3131 3319 4880 5477 6264 6532 1239
Size: 93 188 1561 597 787 268 1524
Fragments arranged by size:
1561 1524 787 597 550 393 301 300
268 188 93 92 84 57 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2114 2126 3388 3455 2114
Size: 12 1262 67 5476
Fragments arranged by size:
5476 1262 67 12
EcoO109I rG'GnC_Cy
Cuts at: 2119 2533 2573 3184 3502 3618 4091 2119
Size: 414 40 611 318 116 473 4845
Fragments arranged by size:
4845 611 473 414 318 116 40
EcoRI G'AATT_C
Cuts at: 4126 4504 4126
Size: 378 6439
FspI TGC'GCA
Cuts at: 5323 5323
Size: 6817
HaeII r_GCGC'y
Cuts at: 1678 2863 3791 3968 5964 1678
Size: 1185 928 177 1996 2531
Fragments arranged by size:
2531 1996 1185 928 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2161 2193 2726
Size: 32 135 32 455 32 102 32 533
Cuts at: 2726 2758 3297 3329 3725 3757 4058 4090 5458
Size: 32 539 32 396 32 301 32 1368
Cuts at: 5458 5490 5532 5564 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1368 539 533 455 396 301 135
102 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4161 4407 669
Size: 988 281 2223 246 3079
Fragments arranged by size:
3079 2223 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2299 2636 748
Size: 1551 337 4929
Fragments arranged by size:
4929 1551 337
HpaI GTT'AAC
Cuts at: 4407 4407
Size: 6817
KasI G'GCGC_C
Cuts at: 3964 3964
Size: 6817
KpnI G_GTAC'C
Cuts at: 2070 3430 4142 2070
Size: 1360 712 4745
Fragments arranged by size:
4745 1360 712
MfeI C'AATT_G
Cuts at: 4416 4416
Size: 6817
MluI A'CGCG_T
Cuts at: 6693 6693
Size: 6817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3445 5931 6115 1305
Size: 2140 2486 184 2007
Fragments arranged by size:
2486 2140 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4818
Fragments arranged by size:
4818 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3797 3923 4775 5134 5293 518
Size: 648 2631 126 852 359 159 2042
Fragments arranged by size:
2631 2042 852 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2709 3366 3615 4233 4910 6058 6303 1606
Size: 1103 657 249 618 677 1148 245 2120
Fragments arranged by size:
2120 1148 1103 677 657 618 249 245
NarI GG'CG_CC
Cuts at: 3965 3965
Size: 6817
NcoI C'CATG_G
Cuts at: 513 1065 2004 3693 513
Size: 552 939 1689 3637
Fragments arranged by size:
3637 1689 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6817
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6817
NotI GC'GGCC_GC
Cuts at: 4264 4264
Size: 6817
NspI r_CATG'y
Cuts at: 1132 1229 2144 3494 4175 5720 1132
Size: 97 915 1350 681 1545 2229
Fragments arranged by size:
2229 1545 1350 915 681 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6817
PciI A'CATG_T
Cuts at: 5716 5716
Size: 6817
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3644 1108
Size: 2536 4281
PfoI T'CCnGG_A
Cuts at: 2844 2906 2844
Size: 62 6755
PmeI GTTT'AAAC
Cuts at: 4121 4121
Size: 6817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4886 4918 6399 6431 4886
Size: 32 1481 32 5272
Fragments arranged by size:
5272 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2533 4091 2119
Size: 414 1558 4845
Fragments arranged by size:
4845 1558 414
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6817
PsiI TTA'TAA
Cuts at: 4387 4387
Size: 6817
PspOMI G'GGCC_C
Cuts at: 3617 3617
Size: 6817
PstI C_TGCA'G
Cuts at: 830 3089 3474 3987 4169 830
Size: 2259 385 513 182 3478
Fragments arranged by size:
3478 2259 513 385 182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6817
PvuII CAG'CTG
Cuts at: 3366 3366
Size: 6817
SacI G_AGCT'C
Cuts at: 3684 3728 3684
Size: 44 6773
SacII CC_GC'GG
Cuts at: 3616 3616
Size: 6817
SalI G'TCGA_C
Cuts at: 1936 4159 1936
Size: 2223 4594
SapI GCTCTTCn'nnn_
Cuts at: 2349 3935 2349
Size: 1586 5231
SbfI CC_TGCA'GG
Cuts at: 3474 4169 3474
Size: 695 6122
ScaI AGT'ACT
Cuts at: 3572 5065 6777 3572
Size: 1493 1712 3612
Fragments arranged by size:
3612 1712 1493
SfcI C'TryA_G
Cuts at: 826 1045 3085 3361 3470 3935 3983 4165 5300
Size: 219 2040 276 109 465 48 182 1135
Cuts at: 5300 5981 6172 826
Size: 681 191 1471
Fragments arranged by size:
2040 1471 1135 681 465 276 219 191
182 109 48
SfoI GGC'GCC
Cuts at: 3966 3966
Size: 6817
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6817
SmaI CCC'GGG
Cuts at: 1872 2033 3404 4144 1872
Size: 161 1371 740 4545
Fragments arranged by size:
4545 1371 740 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2296 3152 3719 4921 5822 6084
Size: 197 926 353 856 567 1202 901 262
Cuts at: 6084 820
Size: 1553
Fragments arranged by size:
1553 1202 926 901 856 567 353 262
197
SnaBI TAC'GTA
Cuts at: 493 4608 493
Size: 4115 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6817
SphI G_CATG'C
Cuts at: 1132 2144 4175 1132
Size: 1012 2031 3774
Fragments arranged by size:
3774 2031 1012
SspI AAT'ATT
Cuts at: 5 52 4741 5
Size: 47 4689 2081
Fragments arranged by size:
4689 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6817
StyI C'CwwG_G
Cuts at: 513 1065 2004 2994 3063 3693 4062 4256 513
Size: 552 939 990 69 630 369 194 3074
Fragments arranged by size:
3074 990 939 630 552 369 194 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2425 4844 4861 5014 5199 5644 325
Size: 1255 845 2419 17 153 185 445 1498
Fragments arranged by size:
2419 1498 1255 845 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3210 3570 5063
Size: 274 80 33 51 1443 1233 360 1493
Cuts at: 5063 6775 96
Size: 1712 138
Fragments arranged by size:
1712 1493 1443 1233 360 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 3022 3054 6777 6809 2975
Size: 32 15 32 3723 32 2983
Fragments arranged by size:
3723 2983 32 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6817
XbaI T'CTAG_A
Cuts at: 4153 4153
Size: 6817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3070 3256 3515 3070
Size: 186 259 6372
Fragments arranged by size:
6372 259 186
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6817
XmaI C'CCGG_G
Cuts at: 1870 2031 3402 4142 1870
Size: 161 1371 740 4545
Fragments arranged by size:
4545 1371 740 161
XmnI GAAnn'nnTTC
Cuts at: 4946 4946
Size: 6817
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4625 276
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SexAI SfiI SrfI SwaI