Restriction map for
pFN21AB8001
(Circular) (Six-base) MAPSORT of: pFN21AB8001.seq Check: 9003 from: 1 to: 5500
pFN21AB8001 5500 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 2843 1937
Size: 514 392 4594
Fragments arranged by size:
4594 514 392
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2322 3563 4947 5215 1632
Size: 690 1241 1384 268 1917
Fragments arranged by size:
1917 1384 1241 690 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AlwNI CAG_nnn'CTG
Cuts at: 2300 2711 2753 3243 4815 2300
Size: 411 42 490 1572 2985
Fragments arranged by size:
2985 1572 490 411 42
ApaI G_GGCC'C
Cuts at: 2718 2718
Size: 5500
ApaLI G'TGCA_C
Cuts at: 3557 4713 3557
Size: 1156 4344
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 2825 1870
Size: 73 700 182 4545
Fragments arranged by size:
4545 700 182 73
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2532 2821 4276 618
Size: 325 217 627 745 289 1455 1842
Fragments arranged by size:
1842 1455 745 627 325 289 217
BanII G_rGCy'C
Cuts at: 1391 2190 2718 2819 1391
Size: 799 528 101 4072
Fragments arranged by size:
4072 799 528 101
BbeI G_GCGC'C
Cuts at: 2536 2536
Size: 5500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3691 3725 1903
Size: 34 50 34 1670 34 3678
Fragments arranged by size:
3678 1670 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2233 2748 3392 4602 2233
Size: 515 644 1210 3131
Fragments arranged by size:
3131 1210 644 515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4111 136
Size: 107 122 71 631 605 2439 1525
Fragments arranged by size:
2439 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5495 5495
Size: 5500
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 2651 2718 3561 4717 1790
Size: 861 67 843 1156 2573
Fragments arranged by size:
2573 1156 861 843 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4189 442
Size: 416 53 83 399 24 1814 958 1753
Fragments arranged by size:
1814 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2069
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 4160 1239
Size: 845 2076 2579
Fragments arranged by size:
2579 2076 845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 882
Size: 617 2664 2219
Fragments arranged by size:
2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2652 3291 493
Size: 711 1448 639 2702
Fragments arranged by size:
2702 1448 711 639
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2533 2637 3307 3689
Size: 53 83 186 936 1000 104 670 382
Cuts at: 3689 4324 275
Size: 635 1451
Fragments arranged by size:
1451 1000 936 670 635 382 186 104
83 53
BsaWI w'CCGG_w
Cuts at: 2316 3933 4331 4605 4752 5163 2316
Size: 1617 398 274 147 411 2653
Fragments arranged by size:
2653 1617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 1178
Size: 30 165 30 831 30 4414
Fragments arranged by size:
4414 831 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 1186
Size: 1378 4122
BseYI C'CCAG_C
Cuts at: 2711 4703 5199 2711
Size: 1992 496 3012
Fragments arranged by size:
3012 1992 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4927
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2735 2950 3711 4739 1540
Size: 275 186 734 215 761 1028 2301
Fragments arranged by size:
2301 1028 761 734 275 215 186
BsiHKAI G_wGCw'C
Cuts at: 2760 3561 3646 4717 2760
Size: 801 85 1071 3543
Fragments arranged by size:
3543 1071 801 85
BsmI GAATG_Cn'
Cuts at: 2209 3011 3104 2209
Size: 802 93 4605
Fragments arranged by size:
4605 802 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2651 2718 2760 2819 3561 3646
Size: 399 400 461 67 42 59 742 85
Cuts at: 3646 4717 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 461 400 399 85 67
59 42
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 844
Size: 2011 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3387 1394
Size: 429 1564 3507
Fragments arranged by size:
3507 1564 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 3995 4169 65
Size: 1183 840 1907 174 1396
Fragments arranged by size:
1907 1396 1183 840 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4144 4331 1700
Size: 110 2334 187 2869
Fragments arranged by size:
2869 2334 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5500
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 2257 1184
Size: 1073 4427
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2157 2254 2137
Size: 20 97 5383
Fragments arranged by size:
5383 97 20
BstYI r'GATC_y
Cuts at: 1972 2830 3197 3582 3599 5051 5137 5149 5495
Size: 858 367 385 17 1452 86 12 346
Cuts at: 5495 1972
Size: 1977
Fragments arranged by size:
1977 1452 858 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2253 2491 2613 513
Size: 552 1188 238 122 3400
Fragments arranged by size:
3400 1188 552 238 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2525 487
Size: 683 56 1299 3462
Fragments arranged by size:
3462 1299 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3810 3830 3016
Size: 794 20 4686
Fragments arranged by size:
4686 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 809
Size: 507 3191 1802
Fragments arranged by size:
3191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2268 2732
Size: 54 1425 50 275 18 207 231 464
Cuts at: 2732 2947 3836 5465 8
Size: 215 889 1629 43
Fragments arranged by size:
1629 1425 889 464 275 231 215 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2732 2947 1537
Size: 275 920 215 4090
Fragments arranged by size:
4090 920 275 215
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2511 2595 2726 3430 1353
Size: 723 435 84 131 704 3423
Fragments arranged by size:
3423 723 704 435 131 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4087 4461 4607 387
Size: 1064 138 2498 374 146 1280
Fragments arranged by size:
2498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2322 3563 4160 4947 5215 1239
Size: 393 452 238 1241 597 787 268 1524
Fragments arranged by size:
1524 1241 787 597 452 393 268 238
EcoO109I rG'GnC_Cy
Cuts at: 2143 2715 2143
Size: 572 4928
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2536 2697 4647 1678
Size: 858 161 1950 2531
Fragments arranged by size:
2531 1950 858 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2234 2239 2266 2609
Size: 32 135 32 635 27 5 27 343
Cuts at: 2609 2641 4141 4173 4215 4247 1373
Size: 32 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 635 343 135 42 32 32
32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2844 3090 669
Size: 988 281 720 186 246 3079
Fragments arranged by size:
3079 988 720 281 246 186
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KasI G'GCGC_C
Cuts at: 2532 2532
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2292 2313 2334 4614 4798 1305
Size: 813 174 21 21 2280 184 2007
Fragments arranged by size:
2280 2007 813 184 174 21 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 10
Size: 54 1425 343 207 3471
Fragments arranged by size:
3471 1425 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3458 3817 3976 518
Size: 648 2292 359 159 2042
Fragments arranged by size:
2292 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2493 2711 2731 2916 3593 4741 4986
Size: 405 482 218 20 185 677 1148 245
Cuts at: 4986 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 482 405 245 218 185
20
NarI GG'CG_CC
Cuts at: 2533 2533
Size: 5500
NcoI C'CATG_G
Cuts at: 513 1065 2613 513
Size: 552 1548 3400
Fragments arranged by size:
3400 1548 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2858 4403 1132
Size: 97 1629 1545 2229
Fragments arranged by size:
2229 1629 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2747 1348
Size: 1399 4101
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5500
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PmlI CAC'GTG
Cuts at: 2652 2652
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5500
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PspOMI G'GGCC_C
Cuts at: 2714 2714
Size: 5500
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2153 2185 2153
Size: 32 5468
PstI C_TGCA'G
Cuts at: 830 2028 2852 830
Size: 1198 824 3478
Fragments arranged by size:
3478 1198 824
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
PvuII CAG'CTG
Cuts at: 2711 2711
Size: 5500
RsrII CG'GwC_CG
Cuts at: 2356 2356
Size: 5500
SacII CC_GC'GG
Cuts at: 2494 2494
Size: 5500
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SexAI A'CCwGG_T
Cuts at: 2132 2132
Size: 5500
SfcI C'TryA_G
Cuts at: 826 1045 2024 2848 3983 4664 4855 826
Size: 219 979 824 1135 681 191 1471
Fragments arranged by size:
1471 1135 979 824 681 219 191
SfoI GGC'GCC
Cuts at: 2534 2534
Size: 5500
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2645 2827 1872
Size: 773 182 4545
Fragments arranged by size:
4545 773 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2858 1132
Size: 1726 3774
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 2777 1517
Size: 1260 4240
StyI C'CwwG_G
Cuts at: 513 1065 2613 2939 513
Size: 552 1548 326 3074
Fragments arranged by size:
3074 1548 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3527 3544 3697 3882 4327 325
Size: 1255 1947 17 153 185 445 1498
Fragments arranged by size:
1947 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3746 5458 96
Size: 274 80 33 51 1443 1769 1712 138
Fragments arranged by size:
1769 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5500
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2643 2825 1870
Size: 773 182 4545
Fragments arranged by size:
4545 773 182
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PspXI SacI SanDI SapI SfiI
SrfI SwaI XcmI