Restriction map for
pFN21AB7996
(Circular) (Six-base) MAPSORT of: pFN21AB7996.seq Check: 9081 from: 1 to: 6115
pFN21AB7996 6115 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2491 2713 2734 2743 2491
Size: 222 21 9 5863
Fragments arranged by size:
5863 222 21 9
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3458 1937
Size: 1521 4594
Acc65I G'GTAC_C
Cuts at: 3436 3436
Size: 6115
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 4178 5562 5830 1632
Size: 391 2155 1384 268 1917
Fragments arranged by size:
2155 1917 1384 391 268
AfeI AGC'GCT
Cuts at: 2796 2796
Size: 6115
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 4844 4844
Size: 6115
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2153 1184
Size: 969 5146
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 6083
AlwNI CAG_nnn'CTG
Cuts at: 2305 2446 3858 5430 2305
Size: 141 1412 1572 2990
Fragments arranged by size:
2990 1572 1412 141
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 3424 3650 3802 5911 1493
Size: 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2155 2193 2400 2780 3086 3440
Size: 73 147 65 38 207 380 306 354
Cuts at: 3440 1870
Size: 4545
Fragments arranged by size:
4545 380 354 306 207 147 73 65
38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2044 2077 2044
Size: 33 6082
BamHI G'GATC_C
Cuts at: 3445 3812 3445
Size: 367 5748
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 3126 3436 4891 618
Size: 325 217 627 420 919 310 1455 1842
Fragments arranged by size:
1842 1455 919 627 420 325 310 217
BanII G_rGCy'C
Cuts at: 1391 2147 2162 2316 3150 3434 1391
Size: 756 15 154 834 284 4072
Fragments arranged by size:
4072 834 756 284 154 15
BbeI G_GCGC'C
Cuts at: 2211 2211
Size: 6115
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3317 3390 928
Size: 971 1418 73 3653
Fragments arranged by size:
3653 1418 971 73
BbvCI CC'TCA_GC
Cuts at: 2798 2798
Size: 6115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2824 2858 4306 4340 1903
Size: 34 887 34 1448 34 3678
Fragments arranged by size:
3678 1448 887 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 3321 1548
Size: 1773 4342
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 3053 4726
Size: 107 122 71 631 605 470 911 1673
Cuts at: 4726 136
Size: 1525
Fragments arranged by size:
1673 1525 911 631 605 470 122 107
71
BglII A'GATC_T
Cuts at: 6110 6110
Size: 6115
BlpI GC'TnA_GC
Cuts at: 2390 3532 2390
Size: 1142 4973
Bme1580I G_kGCm'C
Cuts at: 1790 3234 4176 5332 1790
Size: 1444 942 1156 2573
Fragments arranged by size:
2573 1444 1156 942
BmgBI CAC'GTC
Cuts at: 1318 2614 1318
Size: 1296 4819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3846 4804 442
Size: 416 53 83 399 24 2429 958 1753
Fragments arranged by size:
2429 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2378 2489 3053 3071 3224 4775 1239
Size: 871 268 111 564 18 153 1551 2579
Fragments arranged by size:
2579 1551 871 564 268 153 111 18
Bpu10I CC'TnA_GC
Cuts at: 2798 3014 2798
Size: 216 5899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2672 4240 5105 5403 2525
Size: 147 1568 865 298 3237
Fragments arranged by size:
3237 1568 865 298 147
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2709 3329 4778 882
Size: 617 1210 620 1449 2219
Fragments arranged by size:
2219 1449 1210 620 617
BsaAI yAC'GTr
Cuts at: 493 1204 3906 493
Size: 711 2702 2702
Fragments arranged by size:
2702 2702 711
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 2953 3922 4304
Size: 53 83 186 936 675 745 969 382
Cuts at: 4304 4939 275
Size: 635 1451
Fragments arranged by size:
1451 969 936 745 675 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2901 4548 4946 5220 5367 5778 2901
Size: 1647 398 274 147 411 3238
Fragments arranged by size:
3238 1647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2846 2876 3026 3056 1178
Size: 30 165 30 1443 30 150 30 4237
Fragments arranged by size:
4237 1443 165 150 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2152 2570 2573 2717 2720 2870 3050 3104
Size: 966 418 3 144 3 150 180 54
Cuts at: 3104 3107 3128 3140 3227 1186
Size: 3 21 12 87 4074
Fragments arranged by size:
4074 966 418 180 150 144 87 54
21 12 3 3 3
BseYI C'CCAG_C
Cuts at: 2140 5318 5814 2140
Size: 3178 496 2441
Fragments arranged by size:
3178 2441 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 2681 2768 1163
Size: 573 783 162 87 4510
Fragments arranged by size:
4510 783 573 162 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2323 3565 4326 5354 1540
Size: 275 186 322 1242 761 1028 2301
Fragments arranged by size:
2301 1242 1028 761 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2316 2589 2601 3150 4176 4261 5332 2162
Size: 154 273 12 549 1026 85 1071 2945
Fragments arranged by size:
2945 1071 1026 549 273 154 85 12
BsmI GAATG_Cn'
Cuts at: 3626 3719 3626
Size: 93 6022
BsmBI CGTCTCn'nnnn_
Cuts at: 2555 2555
Size: 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2162 2316 2589 2601 3150 3234
Size: 399 357 15 154 273 12 549 84
Cuts at: 3234 3434 4176 4261 5332 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 549 399 357 273 200
154 85 84 15 12
BspEI T'CCGG_A
Cuts at: 5778 5778
Size: 6115
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2491 2713 2727 2734 2743 2850 3470 844
Size: 1647 222 14 7 9 107 620 3489
Fragments arranged by size:
3489 1647 620 222 107 14 9 7
BsrBI CCG'CTC
Cuts at: 1394 1823 2198 2204 4002 1394
Size: 429 375 6 1798 3507
Fragments arranged by size:
3507 1798 429 375 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4610 4784 65
Size: 1183 3362 174 1396
Fragments arranged by size:
3362 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2413 4759 4946 1700
Size: 110 223 380 2346 187 2869
Fragments arranged by size:
2869 2346 380 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 2417 2419 2811 1925
Size: 492 2 392 5229
Fragments arranged by size:
5229 492 392 2
BssSI C'ACGA_G
Cuts at: 2594 2699 2909 4175 5187 2594
Size: 105 210 1266 1012 3522
Fragments arranged by size:
3522 1266 1012 210 105
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2305 2446 794
Size: 1511 141 4463
Fragments arranged by size:
4463 1511 141
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2958 3222 3445 3812 4197 4214 5666 5752
Size: 986 264 223 367 385 17 1452 86
Cuts at: 5752 5764 6110 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 986 385 367 346 264 223
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3131 3276 3131
Size: 145 5970
BtgI C'CryG_G
Cuts at: 513 1065 2247 2769 513
Size: 552 1182 522 3859
Fragments arranged by size:
3859 1182 552 522
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2812 487
Size: 683 56 1586 3790
Fragments arranged by size:
3790 1586 683 56
BtsI GCAGTG_nn'
Cuts at: 3388 3631 4425 4445 3388
Size: 243 794 20 5058
Fragments arranged by size:
5058 794 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2277 3562
Size: 54 1425 50 275 18 420 27 1285
Cuts at: 3562 4451 6080 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1285 889 420 275 54 50
43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3562 1537
Size: 275 1750 4090
Fragments arranged by size:
4090 1750 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 4045 1353
Size: 1253 1439 3423
Fragments arranged by size:
3423 1439 1253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2537 2867 4702 5076 5222 387
Size: 1064 138 948 330 1835 374 146 1280
Fragments arranged by size:
1835 1280 1064 948 374 330 146 138
EcoICRI GAG'CTC
Cuts at: 2160 2314 3148 2160
Size: 154 834 5127
Fragments arranged by size:
5127 834 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2110 2378 2489 3053 3071 3224
Size: 393 391 87 268 111 564 18 153
Cuts at: 3224 4178 4775 5562 5830 1239
Size: 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 597 564 393 391 268
268 153 111 87 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2264 2846 2921 2264
Size: 582 75 5458
Fragments arranged by size:
5458 582 75
EcoO109I rG'GnC_Cy
Cuts at: 2094 2257 2527 2914 2094
Size: 163 270 387 5295
Fragments arranged by size:
5295 387 270 163
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 6115
FspI TGC'GCA
Cuts at: 4621 4621
Size: 6115
HaeII r_GCGC'y
Cuts at: 1678 2211 2236 2540 2798 3011 3078 5262 1678
Size: 533 25 304 258 213 67 2184 2531
Fragments arranged by size:
2531 2184 533 304 258 213 67 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2647 2679 2943 2950 2975
Size: 32 135 32 1075 32 264 7 25
Cuts at: 2975 2982 4756 4788 4830 4862 1373
Size: 7 1774 32 42 32 2626
Fragments arranged by size:
2626 1774 1075 264 135 42 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 3705 669
Size: 988 281 1521 246 3079
Fragments arranged by size:
3079 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6115
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KasI G'GCGC_C
Cuts at: 2207 2207
Size: 6115
KpnI G_GTAC'C
Cuts at: 3440 3440
Size: 6115
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 2913 5229 5413 1305
Size: 1227 381 2316 184 2007
Fragments arranged by size:
2316 2007 1227 381 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 3846
Fragments arranged by size:
3846 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4073 4432 4591 518
Size: 648 2907 359 159 2042
Fragments arranged by size:
2907 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2431 3531 4208 5356 5601 1606
Size: 825 1100 677 1148 245 2120
Fragments arranged by size:
2120 1148 1100 825 677 245
NaeI GCC'GGC
Cuts at: 2035 2415 2035
Size: 380 5735
NarI GG'CG_CC
Cuts at: 2208 2208
Size: 6115
NcoI C'CATG_G
Cuts at: 513 1065 2247 513
Size: 552 1182 4381
Fragments arranged by size:
4381 1182 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6115
NgoMIV G'CCGG_C
Cuts at: 2033 2413 2033
Size: 380 5735
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 3473 5018 1132
Size: 97 2244 1545 2229
Fragments arranged by size:
2244 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6115
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6115
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 3026 3058 4184 4216 5697 5729 2666
Size: 32 328 32 1126 32 1481 32 3052
Fragments arranged by size:
3052 1481 1126 328 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2527
Size: 6115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PspXI vC'TCGA_Gb
Cuts at: 3086 3086
Size: 6115
PstI C_TGCA'G
Cuts at: 830 2310 2724 2847 3467 830
Size: 1480 414 123 620 3478
Fragments arranged by size:
3478 1480 620 414 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 2431 2431
Size: 6115
SacI G_AGCT'C
Cuts at: 2162 2316 3150 2162
Size: 154 834 5127
Fragments arranged by size:
5127 834 154
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SapI GCTCTTCn'nnn_
Cuts at: 2606 2606
Size: 6115
SbfI CC_TGCA'GG
Cuts at: 2724 2847 3467 2724
Size: 123 620 5372
Fragments arranged by size:
5372 620 123
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2306 2720 2843 3463 4598 5279 5470
Size: 219 1261 414 123 620 1135 681 191
Cuts at: 5470 826
Size: 1471
Fragments arranged by size:
1471 1261 1135 681 620 414 219 191
123
SfoI GGC'GCC
Cuts at: 2209 2209
Size: 6115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6115
SmaI CCC'GGG
Cuts at: 1872 2092 2402 3442 1872
Size: 220 310 1040 4545
Fragments arranged by size:
4545 1040 310 220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 2687 3086 4219 5120 5382
Size: 197 926 597 147 399 1133 901 262
Cuts at: 5382 820
Size: 1553
Fragments arranged by size:
1553 1133 926 901 597 399 262 197
147
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 3473 1132
Size: 2341 3774
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 2247 3554 513
Size: 552 1182 1307 3074
Fragments arranged by size:
3074 1307 1182 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2057 4142 4159 4312 4497 4942 325
Size: 1255 477 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 477 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4361 6073 96
Size: 274 80 33 51 1443 2384 1712 138
Fragments arranged by size:
2384 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6075 6107 6075
Size: 32 6083
Tth111I GACn'n_nGTC
Cuts at: 1316 3361 1316
Size: 2045 4070
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 3086 1943
Size: 1143 4972
XmaI C'CCGG_G
Cuts at: 1870 2090 2400 3440 1870
Size: 220 310 1040 4545
Fragments arranged by size:
4545 1040 310 220
XmnI GAAnn'nnTTC
Cuts at: 4244 4244
Size: 6115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BfrBI BplI BsiWI BstXI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI