Restriction map for
pFN21AB7995
(Circular) (Six-base) MAPSORT of: pFN21AB7995.seq Check: 5172 from: 1 to: 6055
pFN21AB7995 6055 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 4118 5502 5770 1632
Size: 590 1896 1384 268 1917
Fragments arranged by size:
1917 1896 1384 590 268
AflII C'TTAA_G
Cuts at: 820 1017 2894 820
Size: 197 1877 3981
Fragments arranged by size:
3981 1877 197
AflIII A'CryG_T
Cuts at: 1203 2232 4954 5931 1203
Size: 1029 2722 977 1327
Fragments arranged by size:
2722 1327 1029 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2238 1184
Size: 1054 5001
AlwNI CAG_nnn'CTG
Cuts at: 3798 5370 3798
Size: 1572 4483
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2319 2697 2747 2939 3364 3590 3742 5851
Size: 826 378 50 192 425 226 152 2109
Cuts at: 5851 1493
Size: 1697
Fragments arranged by size:
2109 1697 826 425 378 226 192 152
50
AseI AT'TA_AT
Cuts at: 160 1032 2528 4609 160
Size: 872 1496 2081 1606
Fragments arranged by size:
2081 1606 1496 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 3380 1870
Size: 73 574 863 4545
Fragments arranged by size:
4545 863 574 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 3376 4831 618
Size: 325 217 627 1079 510 1455 1842
Fragments arranged by size:
1842 1455 1079 627 510 325 217
BanII G_rGCy'C
Cuts at: 1391 2773 3374 1391
Size: 1382 601 4072
Fragments arranged by size:
4072 1382 601
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 2672 928
Size: 971 606 167 4311
Fragments arranged by size:
4311 971 606 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4246 4280 1903
Size: 34 2309 34 3678
Fragments arranged by size:
3678 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BfrBI ATG'CAT
Cuts at: 2089 2426 2795 2089
Size: 337 369 5349
Fragments arranged by size:
5349 369 337
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2409 3317 6050 2409
Size: 908 2733 2414
Fragments arranged by size:
2733 2414 908
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 4116 5272 1790
Size: 2326 1156 2573
Fragments arranged by size:
2573 2326 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2996 3786 4744
Size: 416 53 83 399 24 1579 790 958
Cuts at: 4744 442
Size: 1753
Fragments arranged by size:
1753 1579 958 790 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2724 3109 4715 1239
Size: 1485 385 1606 2579
Fragments arranged by size:
2579 1606 1485 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 5045 5343 4180
Size: 865 298 4892
Fragments arranged by size:
4892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4718 882
Size: 617 3219 2219
Fragments arranged by size:
3219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 3846 493
Size: 711 909 1733 2702
Fragments arranged by size:
2702 1733 909 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2391 3862 4244 4879
Size: 53 83 186 936 858 1471 382 635
Cuts at: 4879 275
Size: 1451
Fragments arranged by size:
1471 1451 936 858 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2373 3258 4488 4886 5160 5307 5718 2373
Size: 885 1230 398 274 147 411 2710
Fragments arranged by size:
2710 1230 885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2485 2515 1178
Size: 30 165 30 1082 30 4718
Fragments arranged by size:
4718 1082 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6055
BseYI C'CCAG_C
Cuts at: 5258 5754 5258
Size: 496 5559
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2100 2271 1163
Size: 573 364 171 4947
Fragments arranged by size:
4947 573 364 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 4116 4201 5272 2073
Size: 2043 85 1071 2856
Fragments arranged by size:
2856 2043 1071 85
BsmI GAATG_Cn'
Cuts at: 2091 2454 3566 3659 2091
Size: 363 1112 93 4487
Fragments arranged by size:
4487 1112 363 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2773 3374 4116 4201 5272 1391
Size: 399 283 700 601 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 700 601 399 283 85
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 2394 3944 5674 2394
Size: 1550 1730 2775
Fragments arranged by size:
2775 1730 1550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3410 844
Size: 2566 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2235 96
Size: 2139 3916
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2409 3317 3385 3752 4137 4154 5606 5692
Size: 437 908 68 367 385 17 1452 86
Cuts at: 5692 5704 6050 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 908 437 385 367 346 86
68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 3571 4365 4385 3571
Size: 794 20 5241
Fragments arranged by size:
5241 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3098 3133 529
Size: 35 2534 35 3451
Fragments arranged by size:
3451 2534 35 35
DraI TTT'AAA
Cuts at: 2406 3270 3359 2406
Size: 864 89 5102
Fragments arranged by size:
5102 864 89
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 3140 3502
Size: 54 1425 50 275 18 294 1016 362
Cuts at: 3502 4391 6020 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1016 889 362 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2720 3108 3308 3985 1353
Size: 1367 388 200 677 3423
Fragments arranged by size:
3423 1367 677 388 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2724 3109 4118 4715 5502 5770
Size: 393 590 502 385 1009 597 787 268
Cuts at: 5770 1239
Size: 1524
Fragments arranged by size:
1524 1009 787 597 590 502 393 385
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2540
Size: 6055
EcoO109I rG'GnC_Cy
Cuts at: 2033 2713 2033
Size: 680 5375
EcoRI G'AATT_C
Cuts at: 2747 2939 3364 3742 2747
Size: 192 425 378 5060
Fragments arranged by size:
5060 425 378 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 6055
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2890 2922 4696 4728 4770
Size: 32 135 32 1318 32 1774 32 42
Cuts at: 4770 4802 1373
Size: 32 2626
Fragments arranged by size:
2626 1774 1318 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2603 3213 748
Size: 1855 610 3590
Fragments arranged by size:
3590 1855 610
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2916 2973 5169 5353 1305
Size: 1611 57 2196 184 2007
Fragments arranged by size:
2196 2007 1611 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 3142 10
Size: 54 1425 343 294 1016 2923
Fragments arranged by size:
2923 1425 1016 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4013 4372 4531 518
Size: 648 2847 359 159 2042
Fragments arranged by size:
2847 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2661 3471 4148 5296 5541 1606
Size: 1055 810 677 1148 245 2120
Fragments arranged by size:
2120 1148 1055 810 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5503
NdeI CA'TA_TG
Cuts at: 387 2797 387
Size: 2410 3645
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NsiI A_TGCA'T
Cuts at: 2091 2428 2797 2091
Size: 337 369 5349
Fragments arranged by size:
5349 369 337
NspI r_CATG'y
Cuts at: 1132 1229 2236 2426 3413 4958 1132
Size: 97 1007 190 987 1545 2229
Fragments arranged by size:
2229 1545 1007 987 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 2232 4954 2232
Size: 2722 3333
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PfoI T'CCnGG_A
Cuts at: 2701 3125 2701
Size: 424 5631
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2713 2713
Size: 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PstI C_TGCA'G
Cuts at: 830 2227 3407 830
Size: 1397 1180 3478
Fragments arranged by size:
3478 1397 1180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2661 2661
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SanDI GG'GwC_CC
Cuts at: 2713 2713
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SexAI A'CCwGG_T
Cuts at: 2573 2573
Size: 6055
SfcI C'TryA_G
Cuts at: 826 1045 2223 3403 4538 5219 5410 826
Size: 219 1178 1180 1135 681 191 1471
Fragments arranged by size:
1471 1180 1178 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 2519 3382 1872
Size: 647 863 4545
Fragments arranged by size:
4545 863 647
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2894 4159 5060 5322 820
Size: 197 926 951 1265 901 262 1553
Fragments arranged by size:
1553 1265 951 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2113 3846 493
Size: 1620 1733 2702
Fragments arranged by size:
2702 1733 1620
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 2386 2526 2680 3979 5
Size: 47 2334 140 154 1299 2081
Fragments arranged by size:
2334 2081 1299 154 140 47
StuI AGG'CCT
Cuts at: 1517 2546 1517
Size: 1029 5026
StyI C'CwwG_G
Cuts at: 513 1065 2096 2253 3494 513
Size: 552 1031 157 1241 3074
Fragments arranged by size:
3074 1241 1031 552 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 4301 6013
Size: 274 80 33 51 1443 258 2066 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
2066 1712 1443 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2485 2517 6015 6047 2485
Size: 32 3498 32 2493
Fragments arranged by size:
3498 2493 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2344
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 2517 3380 1870
Size: 647 863 4545
Fragments arranged by size:
4545 863 647
XmnI GAAnn'nnTTC
Cuts at: 2808 4184 2808
Size: 1376 4679
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SfiI SfoI SrfI SwaI