Restriction map for
pFN21AB7993
(Circular) (Six-base) MAPSORT of: pFN21AB7993.seq Check: 2986 from: 1 to: 6112
pFN21AB7993 6112 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2461 2461
Size: 6112
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 2850 3433 2850
Size: 583 5529
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2615 2862 3380 4175 5559 5827 1632
Size: 370 613 247 518 795 1384 268 1917
Fragments arranged by size:
1917 1384 795 613 518 370 268 247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 2735 1166
Size: 1569 4543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2218 2573 2956 3855 5427 2218
Size: 355 383 899 1572 2903
Fragments arranged by size:
2903 1572 899 383 355
ApaI G_GGCC'C
Cuts at: 2177 2177
Size: 6112
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 3253 3421 3647 3799 5908 1493
Size: 1760 168 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 3299 3437 1870
Size: 73 113 1243 138 4545
Fragments arranged by size:
4545 1243 138 113 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2132 2183 2850 3433
Size: 325 217 627 279 66 51 667 583
Cuts at: 3433 4888 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 667 627 583 325 279 217
66 51
BanII G_rGCy'C
Cuts at: 1391 2087 2177 3357 3431 1391
Size: 696 90 1180 74 4072
Fragments arranged by size:
4072 1180 696 90 74
BbeI G_GCGC'C
Cuts at: 2070 2136 2187 2070
Size: 66 51 5995
Fragments arranged by size:
5995 66 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5141
BbvCI CC'TCA_GC
Cuts at: 2790 2835 3021 2790
Size: 45 186 5881
Fragments arranged by size:
5881 186 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 2124 2181
Size: 107 122 71 631 605 428 24 57
Cuts at: 2181 4723 136
Size: 2542 1525
Fragments arranged by size:
2542 1525 631 605 428 122 107 71
57 24
BglII A'GATC_T
Cuts at: 2721 6107 2721
Size: 3386 2726
BlpI GC'TnA_GC
Cuts at: 2044 3529 2044
Size: 1485 4627
Bme1580I G_kGCm'C
Cuts at: 1790 2177 3406 4173 5329 1790
Size: 387 1229 767 1156 2573
Fragments arranged by size:
2573 1229 1156 767 387
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3843 4801 442
Size: 416 53 83 399 24 2426 958 1753
Fragments arranged by size:
2426 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2106 2106
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2360 2381 2510 2657 2858 3212 3237
Size: 1001 120 21 129 147 201 354 25
Cuts at: 3237 3319 4772 1239
Size: 82 1453 2579
Fragments arranged by size:
2579 1453 1001 354 201 147 129 120
82 25 21
Bpu10I CC'TnA_GC
Cuts at: 2081 2790 2835 3021 3326 2081
Size: 709 45 186 305 4867
Fragments arranged by size:
4867 709 305 186 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 3029 4237 5102 5400 2480
Size: 549 1208 865 298 3192
Fragments arranged by size:
3192 1208 865 549 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 2861 4775 882
Size: 617 858 504 1914 2219
Fragments arranged by size:
2219 1914 858 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2133 2184 2241
Size: 53 83 186 936 534 66 51 57
Cuts at: 2241 3919 4301 4936 275
Size: 1678 382 635 1451
Fragments arranged by size:
1678 1451 936 635 534 382 186 83
66 57 53 51
BsaWI w'CCGG_w
Cuts at: 2907 3375 4545 4943 5217 5364 5775 2907
Size: 468 1170 398 274 147 411 3244
Fragments arranged by size:
3244 1170 468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2354 2378 2384
Size: 30 165 30 603 30 318 24 6
Cuts at: 2384 2408 2747 2777 3194 3210 3224 3240 1178
Size: 24 339 30 417 16 14 16 4050
Fragments arranged by size:
4050 603 417 339 318 165 30 30
30 30 24 24 16 16 14 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2825 3038 3083 3215 1186
Size: 1102 537 213 45 132 4083
Fragments arranged by size:
4083 1102 537 213 132 45
BseYI C'CCAG_C
Cuts at: 2885 5315 5811 2885
Size: 2430 496 3186
Fragments arranged by size:
3186 2430 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5539
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2144 3562 4323 5351 1540
Size: 275 186 143 1418 761 1028 2301
Fragments arranged by size:
2301 1418 1028 761 275 186 143
BsiHKAI G_wGCw'C
Cuts at: 2087 4173 4258 5329 2087
Size: 2086 85 1071 2870
Fragments arranged by size:
2870 2086 1071 85
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
BsmBI CGTCTCn'nnnn_
Cuts at: 2687 2687
Size: 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2177 3357 3406 3431 4173 4258
Size: 399 297 90 1180 49 25 742 85
Cuts at: 4258 5329 1391
Size: 1071 2174
Fragments arranged by size:
2174 1180 1071 742 399 297 90 85
49 25
BspEI T'CCGG_A
Cuts at: 2907 3375 5775 2907
Size: 468 2400 3244
Fragments arranged by size:
3244 2400 468
BspHI T'CATG_A
Cuts at: 3138 4001 5731 3138
Size: 863 1730 3519
Fragments arranged by size:
3519 1730 863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2461 3467 844
Size: 1617 1006 3489
Fragments arranged by size:
3489 1617 1006
BsrBI CCG'CTC
Cuts at: 1394 1823 2090 3999 1394
Size: 429 267 1909 3507
Fragments arranged by size:
3507 1909 429 267
BsrDI GCAATG_nn'
Cuts at: 65 1248 3009 3325 4607 4781 65
Size: 1183 1761 316 1282 174 1396
Fragments arranged by size:
1761 1396 1282 1183 316 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 3207 4756 4943 1700
Size: 110 376 1021 1549 187 2869
Fragments arranged by size:
2869 1549 1021 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 3054 4172 5184 3054
Size: 1118 1012 3982
Fragments arranged by size:
3982 1118 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 2200 1184
Size: 1016 5096
BstXI CCAn_nnnn'nTGG
Cuts at: 2441 2441
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 2721 3442 3809 4194 4211 5663 5749 5761
Size: 749 721 367 385 17 1452 86 12
Cuts at: 5761 6107 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 749 721 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2678 2678
Size: 6112
BtgI C'CryG_G
Cuts at: 513 1065 2062 2748 3309 513
Size: 552 997 686 561 3316
Fragments arranged by size:
3316 997 686 561 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2770 487
Size: 683 56 1544 3829
Fragments arranged by size:
3829 1544 683 56
BtsI GCAGTG_nn'
Cuts at: 2465 3280 3346 3628 4422 4442 2465
Size: 815 66 282 794 20 4135
Fragments arranged by size:
4135 815 794 282 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2141 2151 2397
Size: 54 1425 50 275 18 311 10 246
Cuts at: 2397 2817 3559 4448 6077 8
Size: 420 742 889 1629 43
Fragments arranged by size:
1629 1425 889 742 420 311 275 246
54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2141 3559 1537
Size: 275 329 1418 4090
Fragments arranged by size:
4090 1418 329 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4042 1353
Size: 2689 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 3376 4699 5073 5219 387
Size: 1064 138 921 866 1323 374 146 1280
Fragments arranged by size:
1323 1280 1064 921 866 374 146 138
EcoICRI GAG'CTC
Cuts at: 2085 2085
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2240 2360 2381 2510 2615 2657
Size: 393 370 238 120 21 129 105 42
Cuts at: 2657 2858 2862 3212 3237 3319 3380 4175 4772
Size: 201 4 350 25 82 61 795 597
Cuts at: 4772 5559 5827 1239
Size: 787 268 1524
Fragments arranged by size:
1524 795 787 597 393 370 350 268
238 201 129 120 105 82 61 42
25 21 4
EcoO109I rG'GnC_Cy
Cuts at: 2173 2682 3347 2173
Size: 509 665 4938
Fragments arranged by size:
4938 665 509
EcoRI G'AATT_C
Cuts at: 3253 3421 3799 3253
Size: 168 378 5566
Fragments arranged by size:
5566 378 168
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2070 2136 2187 3044 5259 1678
Size: 392 66 51 857 2215 2531
Fragments arranged by size:
2531 2215 857 392 66 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 2699 2731 4753
Size: 32 135 32 806 32 289 32 2022
Cuts at: 4753 4785 4827 4859 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2022 806 289 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 3456 3702 669
Size: 988 281 765 753 246 3079
Fragments arranged by size:
3079 988 765 753 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KasI G'GCGC_C
Cuts at: 2066 2132 2183 2066
Size: 66 51 5995
Fragments arranged by size:
5995 66 51
KpnI G_GTAC'C
Cuts at: 2854 3437 2854
Size: 583 5529
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2751 2979 5226 5410 1305
Size: 1446 228 2247 184 2007
Fragments arranged by size:
2247 2007 1446 228 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2819 10
Size: 54 1425 343 987 3303
Fragments arranged by size:
3303 1425 987 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2735 3143 4070 4429 4588 518
Size: 648 1569 408 927 359 159 2042
Fragments arranged by size:
2042 1569 927 648 408 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2230 2260 2570 3091 3118 3528 4205
Size: 458 166 30 310 521 27 410 677
Cuts at: 4205 5353 5598 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 521 458 410 310 245
166 30 27
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 6112
NarI GG'CG_CC
Cuts at: 2067 2133 2184 2067
Size: 66 51 5995
Fragments arranged by size:
5995 66 51
NcoI C'CATG_G
Cuts at: 513 1065 2748 3309 513
Size: 552 1683 561 3316
Fragments arranged by size:
3316 1683 561 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 2141 3559 2141
Size: 1418 4694
NspI r_CATG'y
Cuts at: 1132 1229 2512 3470 5015 1132
Size: 97 1283 958 1545 2229
Fragments arranged by size:
2229 1545 1283 958 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2682 3347 2682
Size: 665 5447
PshAI GACnn'nnGTC
Cuts at: 1613 2757 1613
Size: 1144 4968
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PspOMI G'GGCC_C
Cuts at: 2173 2173
Size: 6112
PstI C_TGCA'G
Cuts at: 830 2385 2472 2715 2961 3090 3464 830
Size: 1555 87 243 246 129 374 3478
Fragments arranged by size:
3478 1555 374 246 243 129 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2570 3118 2570
Size: 548 5564
RsrII CG'GwC_CG
Cuts at: 3378 3378
Size: 6112
SacI G_AGCT'C
Cuts at: 2087 2087
Size: 6112
SacII CC_GC'GG
Cuts at: 2065 2065
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2381 2468 2711 2957 3086 3460 4595
Size: 219 1336 87 243 246 129 374 1135
Cuts at: 4595 5276 5467 826
Size: 681 191 1471
Fragments arranged by size:
1471 1336 1135 681 374 246 243 219
191 129 87
SfoI GGC'GCC
Cuts at: 2068 2134 2185 2068
Size: 66 51 5995
Fragments arranged by size:
5995 66 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2459 3044 4216 5117 5379 820
Size: 197 926 516 585 1172 901 262 1553
Fragments arranged by size:
1553 1172 926 901 585 516 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 2512 3470 1132
Size: 1380 958 3774
Fragments arranged by size:
3774 1380 958
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2104 2643 2748 3309 3551 513
Size: 552 1039 539 105 561 242 3074
Fragments arranged by size:
3074 1039 561 552 539 242 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4139 4156 4309 4494 4939 325
Size: 1255 2559 17 153 185 445 1498
Fragments arranged by size:
2559 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 2354 2386 3210 3242 6072 6104 2006
Size: 32 316 32 824 32 2830 32 2014
Fragments arranged by size:
2830 2014 824 316 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2882 3103 2882
Size: 221 5891
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI SanDI SapI SexAI
SfiI SrfI SwaI