Restriction map for
pFN21AB7992
(Circular) (Six-base) MAPSORT of: pFN21AB7992.seq Check: 770 from: 1 to: 6010
pFN21AB7992 6010 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 2762 3331 2762
Size: 569 5441
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2233 2712 3064 4073 5457 5725 1632
Size: 476 125 479 352 1009 1384 268 1917
Fragments arranged by size:
1917 1384 1009 479 476 352 268 125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 4909 5886 1203
Size: 3706 977 1327
Fragments arranged by size:
3706 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 2277 4739 2277
Size: 2462 3548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6010
AlwNI CAG_nnn'CTG
Cuts at: 2108 2359 2513 3753 5325 2108
Size: 251 154 1240 1572 2793
Fragments arranged by size:
2793 1572 1240 251 154
ApaI G_GGCC'C
Cuts at: 2034 2034
Size: 6010
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 2862 3030 3198 3319 3545 3697 5806 1493
Size: 1369 168 168 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1369 226 168 168 152 121
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2283 3335 1870
Size: 73 340 1052 4545
Fragments arranged by size:
4545 1052 340 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2762 3331 4786 618
Size: 325 217 627 975 569 1455 1842
Fragments arranged by size:
1842 1455 975 627 569 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2409 2655 3008 3267 3329 1391
Size: 643 375 246 353 259 62 4072
Fragments arranged by size:
4072 643 375 353 259 246 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5039
BbvCI CC'TCA_GC
Cuts at: 2327 2327
Size: 6010
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2295 2329 4201 4235 1903
Size: 34 358 34 1872 34 3678
Fragments arranged by size:
3678 1872 358 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4621 136
Size: 107 122 71 631 605 2949 1525
Fragments arranged by size:
2949 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2232 6005 2232
Size: 3773 2237
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2034 4071 5227 1790
Size: 244 2037 1156 2573
Fragments arranged by size:
2573 2037 1156 244
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3741 4699 442
Size: 416 53 83 399 24 2324 958 1753
Fragments arranged by size:
2324 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2486 2579 2976 4670 1239
Size: 1142 105 93 397 1694 2579
Fragments arranged by size:
2579 1694 1142 397 105 93
Bpu10I CC'TnA_GC
Cuts at: 2327 2327
Size: 6010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2314 4135 5000 5298 2314
Size: 1821 865 298 3026
Fragments arranged by size:
3026 1821 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2189 2600 4673 882
Size: 617 690 411 2073 2219
Fragments arranged by size:
2219 2073 690 617 411
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3817 4199 4834 275
Size: 53 83 186 936 2284 382 635 1451
Fragments arranged by size:
2284 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2462 1186
Size: 1231 45 4734
Fragments arranged by size:
4734 1231 45
BseYI C'CCAG_C
Cuts at: 2610 5213 5709 2610
Size: 2603 496 2911
Fragments arranged by size:
2911 2603 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2499 2511 1163
Size: 573 360 403 12 4662
Fragments arranged by size:
4662 573 403 360 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 3008 3094 3260 3267 4071 4156 5227 2409
Size: 599 86 166 7 804 85 1071 3192
Fragments arranged by size:
3192 1071 804 599 166 86 85 7
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2409 2655 3008 3094 3260 3267
Size: 399 244 375 246 353 86 166 7
Cuts at: 3267 3329 4071 4156 5227 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 353 246 244
166 86 85 62 7
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2491 2533 3365 844
Size: 1647 42 832 3489
Fragments arranged by size:
3489 1647 832 42
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2669 4505 4679 65
Size: 1183 1421 1836 174 1396
Fragments arranged by size:
1836 1421 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4654 4841 1700
Size: 110 2844 187 2869
Fragments arranged by size:
2869 2844 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6010
BstBI TT'CG_AA
Cuts at: 3054 4814 3054
Size: 1760 4250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2232 2355 3340 3707 4092 4109 5561 5647
Size: 260 123 985 367 385 17 1452 86
Cuts at: 5647 5659 6005 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 985 385 367 346 260 123
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2400 2601 2400
Size: 201 5809
BtgI C'CryG_G
Cuts at: 513 1065 2004 2540 513
Size: 552 939 536 3983
Fragments arranged by size:
3983 939 552 536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 3526 4320 4340 3526
Size: 794 20 5196
Fragments arranged by size:
5196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3457 4346 5975
Size: 54 1425 50 275 18 1627 889 1629
Cuts at: 5975 8
Size: 43
Fragments arranged by size:
1629 1627 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 2529 3940 1353
Size: 813 363 1411 3423
Fragments arranged by size:
3423 1411 813 363
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2228 4597 4971 5117 387
Size: 1064 138 639 2369 374 146 1280
Fragments arranged by size:
2369 1280 1064 639 374 146 138
EcoICRI GAG'CTC
Cuts at: 2407 3006 3265 2407
Size: 599 259 5152
Fragments arranged by size:
5152 599 259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2233 2381 2486 2579 2712 2976
Size: 393 476 125 148 105 93 133 264
Cuts at: 2976 3064 4073 4670 5457 5725 1239
Size: 88 1009 597 787 268 1524
Fragments arranged by size:
1524 1009 787 597 476 393 268 264
148 133 125 105 93 88
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2399
Size: 6010
EcoO109I rG'GnC_Cy
Cuts at: 2030 2031 2503 2510 2030
Size: 1 472 7 5530
Fragments arranged by size:
5530 472 7 1
EcoRI G'AATT_C
Cuts at: 3030 3198 3319 3697 3030
Size: 168 121 378 5343
Fragments arranged by size:
5343 378 168 121
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 5157 1678
Size: 3479 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2263 2295 2396
Size: 32 135 32 593 32 66 32 101
Cuts at: 2396 2428 2651 2683 4651 4683 4725 4757 1373
Size: 32 223 32 1968 32 42 32 2626
Fragments arranged by size:
2626 1968 593 223 135 101 66 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3243 748
Size: 2495 3515
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 2766 3335 2766
Size: 569 5441
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5124 5308 1305
Size: 3819 184 2007
Fragments arranged by size:
3819 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4188
Fragments arranged by size:
4188 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3968 4327 4486 518
Size: 648 2802 359 159 2042
Fragments arranged by size:
2802 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2108 3426 4103 5251 5496 1606
Size: 443 59 1318 677 1148 245 2120
Fragments arranged by size:
2120 1318 1148 677 443 245 59
NcoI C'CATG_G
Cuts at: 513 1065 2004 2540 513
Size: 552 939 536 3983
Fragments arranged by size:
3983 939 552 536
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 3368 4913 1132
Size: 97 2139 1545 2229
Fragments arranged by size:
2229 2139 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 4909 4909
Size: 6010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6010
PfoI T'CCnGG_A
Cuts at: 2358 2358
Size: 6010
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2503
Size: 6010
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 2919 3580 2919
Size: 661 5349
PspOMI G'GGCC_C
Cuts at: 2030 2030
Size: 6010
PstI C_TGCA'G
Cuts at: 830 2068 2778 3362 830
Size: 1238 710 584 3478
Fragments arranged by size:
3478 1238 710 584
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2049 2108 2049
Size: 59 5951
SacI G_AGCT'C
Cuts at: 2409 3008 3267 2409
Size: 599 259 5152
Fragments arranged by size:
5152 599 259
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SapI GCTCTTCn'nnn_
Cuts at: 2529 2529
Size: 6010
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 2310 4258 5970 2310
Size: 1948 1712 2350
Fragments arranged by size:
2350 1948 1712
SfcI C'TryA_G
Cuts at: 826 1045 2064 2774 3358 4493 5174 5365 826
Size: 219 1019 710 584 1135 681 191 1471
Fragments arranged by size:
1471 1135 1019 710 681 584 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 2285 3337 1872
Size: 413 1052 4545
Fragments arranged by size:
4545 1052 413
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2293 4114 5015 5277 820
Size: 197 926 350 1821 901 262 1553
Fragments arranged by size:
1821 1553 926 901 350 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 2291 3077 3161 1517
Size: 774 786 84 4366
Fragments arranged by size:
4366 786 774 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2540 2655 3449 513
Size: 552 939 536 115 794 3074
Fragments arranged by size:
3074 939 794 552 536 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2407 2698 3005 4037 4054 4207 4392
Size: 1255 827 291 307 1032 17 153 185
Cuts at: 4392 4837 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1032 827 445 307 291 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2308 4256 5968
Size: 274 80 33 51 1443 331 1948 1712
Cuts at: 5968 96
Size: 138
Fragments arranged by size:
1948 1712 1443 331 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2571 2603 5970 6002 2571
Size: 32 3367 32 2579
Fragments arranged by size:
3367 2579 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 2692 2273
Size: 419 5591
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 2283 3335 1870
Size: 413 1052 4545
Fragments arranged by size:
4545 1052 413
XmnI GAAnn'nnTTC
Cuts at: 2993 3050 3077 4139 2993
Size: 57 27 1062 4864
Fragments arranged by size:
4864 1062 57 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI