Restriction map for
pFN21AB7991
(Circular) (Six-base) MAPSORT of: pFN21AB7991.seq Check: 4392 from: 1 to: 6031
pFN21AB7991 6031 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2115 3374 1937
Size: 178 1259 4594
Fragments arranged by size:
4594 1259 178
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2991 3233 4094 5478 5746 1632
Size: 1359 242 861 1384 268 1917
Fragments arranged by size:
1917 1384 1359 861 268 242
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6031
AlwNI CAG_nnn'CTG
Cuts at: 3774 5346 3774
Size: 1572 4459
ApaI G_GGCC'C
Cuts at: 2244 3186 2244
Size: 942 5089
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 3340 3566 3718 5827 1493
Size: 1847 226 152 2109 1697
Fragments arranged by size:
2109 1847 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2455 2455
Size: 6031
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 2203 2243 2263 2335 2382 3356 1870
Size: 73 260 40 20 72 47 974 4545
Fragments arranged by size:
4545 974 260 73 72 47 40 20
BamHI G'GATC_C
Cuts at: 2946 3361 3728 2946
Size: 415 367 5249
Fragments arranged by size:
5249 415 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2075 2096 2134 2198
Size: 325 217 627 264 24 21 38 64
Cuts at: 2198 2850 3352 4807 618
Size: 652 502 1455 1842
Fragments arranged by size:
1842 1455 652 627 502 325 264 217
64 38 24 21
BanII G_rGCy'C
Cuts at: 1391 2186 2244 2474 3186 3350 1391
Size: 795 58 230 712 164 4072
Fragments arranged by size:
4072 795 712 230 164 58
BbeI G_GCGC'C
Cuts at: 2079 2100 2202 2854 2079
Size: 21 102 652 5256
Fragments arranged by size:
5256 652 102 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5060
BbvCI CC'TCA_GC
Cuts at: 2775 2775
Size: 6031
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4222 4256 1903
Size: 34 2285 34 3678
Fragments arranged by size:
3678 2285 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2436 2583
Size: 107 122 71 631 605 388 376 147
Cuts at: 2583 2597 2805 4642 136
Size: 14 208 1837 1525
Fragments arranged by size:
1837 1525 631 605 388 376 208 147
122 107 71 14
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 3448 3448
Size: 6031
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2139 2244 3132 3186 4092 5248 1790
Size: 334 15 105 888 54 906 1156 2573
Fragments arranged by size:
2573 1156 906 888 334 105 54 15
BmgBI CAC'GTC
Cuts at: 1318 2509 1318
Size: 1191 4840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3762 4720 442
Size: 416 53 83 399 24 2345 958 1753
Fragments arranged by size:
2345 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 3042 2825
Size: 217 5814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2464 2707 3037 4691 1239
Size: 911 314 243 330 1654 2579
Fragments arranged by size:
2579 1654 911 330 314 243
Bpu10I CC'TnA_GC
Cuts at: 2775 2775
Size: 6031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4156 5021 5319 4156
Size: 865 298 4868
Fragments arranged by size:
4868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4694 882
Size: 617 3195 2219
Fragments arranged by size:
3195 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3822 493
Size: 711 915 1703 2702
Fragments arranged by size:
2702 1703 915 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2097 2199 2851
Size: 53 83 186 936 543 21 102 652
Cuts at: 2851 3838 4220 4855 275
Size: 987 382 635 1451
Fragments arranged by size:
1451 987 936 652 635 543 382 186
102 83 53 21
BsaWI w'CCGG_w
Cuts at: 2192 2216 4464 4862 5136 5283 5694 2192
Size: 24 2248 398 274 147 411 2529
Fragments arranged by size:
2529 2248 411 398 274 147 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3122 3152 1178
Size: 30 165 30 1719 30 4057
Fragments arranged by size:
4057 1719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 3095 1186
Size: 945 964 4122
Fragments arranged by size:
4122 964 945
BseYI C'CCAG_C
Cuts at: 2149 2250 5234 5730 2149
Size: 101 2984 496 2450
Fragments arranged by size:
2984 2450 496 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 1163
Size: 573 819 4639
Fragments arranged by size:
4639 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2361 2587 2601 2762 3481 4242
Size: 275 186 360 226 14 161 719 761
Cuts at: 4242 5270 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 719 360 275 226 186
161 14
BsiHKAI G_wGCw'C
Cuts at: 4092 4177 5248 4092
Size: 85 1071 4875
Fragments arranged by size:
4875 1071 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 3243 3243
Size: 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2139 2186 2244 2474 3132 3186
Size: 399 334 15 47 58 230 658 54
Cuts at: 3186 3350 4092 4177 5248 1391
Size: 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 399 334 230 164
85 58 54 47 15
BspEI T'CCGG_A
Cuts at: 2192 5694 2192
Size: 3502 2529
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3386 844
Size: 2542 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2417 2683 2764 3918 1394
Size: 429 594 266 81 1154 3507
Fragments arranged by size:
3507 1154 594 429 266 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 2863 2887 2937 4526 4700 65
Size: 1183 1615 24 50 1589 174 1396
Fragments arranged by size:
1615 1589 1396 1183 174 50 24
BsrFI r'CCGG_y
Cuts at: 1700 1810 2311 2459 2546 2757 2781 4675 4862
Size: 110 501 148 87 211 24 1894 187
Cuts at: 4862 1700
Size: 2869
Fragments arranged by size:
2869 1894 501 211 187 148 110 87
24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 2455 1925
Size: 530 5501
BssSI C'ACGA_G
Cuts at: 4091 5103 4091
Size: 1012 5019
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2578 2724 2886 794
Size: 1784 146 162 3939
Fragments arranged by size:
3939 1784 162 146
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 2946 3361 3728 4113 4130 5582 5668 5680
Size: 974 415 367 385 17 1452 86 12
Cuts at: 5680 6026 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 974 415 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2035 2129 2376 2908 3178 513
Size: 552 957 13 94 247 532 270 3366
Fragments arranged by size:
3366 957 552 532 270 247 94 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3182 487
Size: 683 56 1956 3336
Fragments arranged by size:
3336 1956 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 3019 3324 3547 4341 4361 2410
Size: 609 305 223 794 20 4080
Fragments arranged by size:
4080 794 609 305 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DraIII CAC_nnn'GTG
Cuts at: 2847 2847
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2347 2564 5038 809
Size: 507 1031 217 2474 1802
Fragments arranged by size:
2474 1802 1031 507 217
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2025 2358
Size: 54 1425 50 275 18 189 6 333
Cuts at: 2358 2502 2584 2598 2759 3175 3300 3478 4367
Size: 144 82 14 161 416 125 178 889
Cuts at: 4367 5996 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 416 333 275 189 178
161 144 125 82 54 50 43 18
14 6
EagI C'GGCC_G
Cuts at: 1537 1812 2358 2584 2598 2759 3478 1537
Size: 275 546 226 14 161 719 4090
Fragments arranged by size:
4090 719 546 275 226 161 14
EarI CTCTTCn'nnn_
Cuts at: 1353 3961 1353
Size: 2608 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2187 2427 2533 2779 4618 4992
Size: 1064 138 598 240 106 246 1839 374
Cuts at: 4992 5138 387
Size: 146 1280
Fragments arranged by size:
1839 1280 1064 598 374 246 240 146
138 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2464 2707 2991 3037 3233 4094
Size: 393 518 314 243 284 46 196 861
Cuts at: 4094 4691 5478 5746 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 861 787 597 518 393 314 284
268 243 196 46
EcoO109I rG'GnC_Cy
Cuts at: 2240 2247 2349 2914 3183 2240
Size: 7 102 565 269 5088
Fragments arranged by size:
5088 565 269 102 7
EcoRI G'AATT_C
Cuts at: 3340 3718 3340
Size: 378 5653
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 2079 2100 2202 2782 2854 5178 1678
Size: 401 21 102 580 72 2324 2531
Fragments arranged by size:
2531 2324 580 401 102 72 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2640 2653 2672 4672
Size: 32 135 32 1049 19 13 19 2000
Cuts at: 4672 4704 4746 4778 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2000 1049 135 42 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2659 3375 3621 669
Size: 988 281 721 716 246 3079
Fragments arranged by size:
3079 988 721 716 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KasI G'GCGC_C
Cuts at: 2075 2096 2198 2850 2075
Size: 21 102 652 5256
Fragments arranged by size:
5256 652 102 21
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5145 5329 1305
Size: 3840 184 2007
Fragments arranged by size:
3840 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 3177 10
Size: 54 1425 343 189 1156 2864
Fragments arranged by size:
2864 1425 1156 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3989 4348 4507 518
Size: 648 2823 359 159 2042
Fragments arranged by size:
2823 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2131 2372 2378 3036 3447 4124 5272 5517
Size: 525 241 6 658 411 677 1148 245
Cuts at: 5517 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 658 525 411 245 241
6
NaeI GCC'GGC
Cuts at: 2461 2548 2783 2461
Size: 87 235 5709
Fragments arranged by size:
5709 235 87
NarI GG'CG_CC
Cuts at: 2076 2097 2199 2851 2076
Size: 21 102 652 5256
Fragments arranged by size:
5256 652 102 21
NcoI C'CATG_G
Cuts at: 513 1065 2022 3178 513
Size: 552 957 1156 3366
Fragments arranged by size:
3366 1156 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NgoMIV G'CCGG_C
Cuts at: 2459 2546 2781 2459
Size: 87 235 5709
Fragments arranged by size:
5709 235 87
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 2358 3478 2358
Size: 1120 4911
NspI r_CATG'y
Cuts at: 1132 1229 3389 4934 1132
Size: 97 2160 1545 2229
Fragments arranged by size:
2229 2160 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6031
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2847 1108
Size: 1739 4292
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2349 2914 2247
Size: 102 565 5364
Fragments arranged by size:
5364 565 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PspOMI G'GGCC_C
Cuts at: 2240 3182 2240
Size: 942 5089
PstI C_TGCA'G
Cuts at: 830 3383 830
Size: 2553 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
SacII CC_GC'GG
Cuts at: 2132 2379 2132
Size: 247 5784
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SanDI GG'GwC_CC
Cuts at: 2247 2349 2914 2247
Size: 102 565 5364
Fragments arranged by size:
5364 565 102
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 2398 3379 4514 5195 5386 826
Size: 219 1353 981 1135 681 191 1471
Fragments arranged by size:
1471 1353 1135 981 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2436 2597 2436
Size: 161 5870
SfoI GGC'GCC
Cuts at: 2077 2098 2200 2852 2077
Size: 21 102 652 5256
Fragments arranged by size:
5256 652 102 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 2311 1700
Size: 611 5420
SmaI CCC'GGG
Cuts at: 1872 2245 2265 2337 2384 3358 1872
Size: 373 20 72 47 974 4545
Fragments arranged by size:
4545 974 373 72 47 20
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4135 5036 5298 820
Size: 197 926 2192 901 262 1553
Fragments arranged by size:
2192 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 2923 1517
Size: 1406 4625
StyI C'CwwG_G
Cuts at: 513 1065 2022 3178 3470 513
Size: 552 957 1156 292 3074
Fragments arranged by size:
3074 1156 957 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4058 4075 4228 4413 4858 325
Size: 1255 2478 17 153 185 445 1498
Fragments arranged by size:
2478 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4277 5989 96
Size: 274 80 33 51 1443 2300 1712 138
Fragments arranged by size:
2300 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3122 3154 5991 6023 3122
Size: 32 2837 32 3130
Fragments arranged by size:
3130 2837 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 2243 2263 2335 2382 3356 1870
Size: 373 20 72 47 974 4545
Fragments arranged by size:
4545 974 373 72 47 20
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SapI SexAI SrfI SwaI