Restriction map for
pFN21AB7990
(Circular) (Six-base) MAPSORT of: pFN21AB7990.seq Check: 706 from: 1 to: 5683
pFN21AB7990 5683 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2824 2824
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 3746 5130 5398 1632
Size: 756 1358 1384 268 1917
Fragments arranged by size:
1917 1384 1358 756 268
AfeI AGC'GCT
Cuts at: 2732 2732
Size: 5683
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 2038 4412 2038
Size: 2374 3309
AleI CACnn'nnGTG
Cuts at: 1166 2330 1166
Size: 1164 4519
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2440 1184
Size: 1256 4427
AlwNI CAG_nnn'CTG
Cuts at: 2182 2615 3426 4998 2182
Size: 433 811 1572 2867
Fragments arranged by size:
2867 1572 811 433
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2108 2992 3218 3370 5479 1493
Size: 615 884 226 152 2109 1697
Fragments arranged by size:
2109 1697 884 615 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2043 2588 3008 1870
Size: 73 78 22 545 420 4545
Fragments arranged by size:
4545 545 420 78 73 22
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2831 3004 4459 618
Size: 325 217 627 537 507 173 1455 1842
Fragments arranged by size:
1842 1455 627 537 507 325 217 173
BanII G_rGCy'C
Cuts at: 1391 2679 2784 3002 1391
Size: 1288 105 218 4072
Fragments arranged by size:
4072 1288 218 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2839 928
Size: 971 940 3772
Fragments arranged by size:
3772 971 940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2208 2310 1548
Size: 660 102 4921
Fragments arranged by size:
4921 660 102
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 2517 3100 2517
Size: 583 5100
Bme1580I G_kGCm'C
Cuts at: 1790 2834 3744 4900 1790
Size: 1044 910 1156 2573
Fragments arranged by size:
2573 1156 1044 910
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2564 3414 4372
Size: 416 53 83 399 24 1147 850 958
Cuts at: 4372 442
Size: 1753
Fragments arranged by size:
1753 1147 958 850 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2501 2564 2888 4343 1239
Size: 829 433 63 324 1455 2579
Fragments arranged by size:
2579 1455 829 433 324 63
Bpu10I CC'TnA_GC
Cuts at: 2131 2131
Size: 5683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2437 3490 3872 4507
Size: 53 83 186 936 904 1053 382 635
Cuts at: 4507 275
Size: 1451
Fragments arranged by size:
1451 1053 936 904 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2507 2537 2567 2861
Size: 30 165 30 1074 30 30 30 294
Cuts at: 2861 2891 1178
Size: 30 3970
Fragments arranged by size:
3970 1074 294 165 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2359 2387 2390 2489 2498 2501 2606 2792
Size: 1173 28 3 99 9 3 105 186
Cuts at: 2792 1186
Size: 4077
Fragments arranged by size:
4077 1173 186 105 99 28 9 3
3
BseYI C'CCAG_C
Cuts at: 2608 2727 2759 4886 5382 2608
Size: 119 32 2127 496 2909
Fragments arranged by size:
2909 2127 496 119 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 2771 1163
Size: 573 480 555 4075
Fragments arranged by size:
4075 573 555 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2603 3133 3894 4922 1540
Size: 275 186 602 530 761 1028 2301
Fragments arranged by size:
2301 1028 761 602 530 275 186
BsiHKAI G_wGCw'C
Cuts at: 3744 3829 4900 3744
Size: 85 1071 4527
Fragments arranged by size:
4527 1071 85
BsmI GAATG_Cn'
Cuts at: 2141 3194 3287 2141
Size: 1053 93 4537
Fragments arranged by size:
4537 1053 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2679 2784 2834 3002 3744 3829 4900
Size: 399 889 105 50 168 742 85 1071
Cuts at: 4900 1391
Size: 2174
Fragments arranged by size:
2174 1071 889 742 399 168 105 85
50
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 2824 3038 844
Size: 1346 634 214 3489
Fragments arranged by size:
3489 1346 634 214
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2754 4178 4352 65
Size: 1183 1506 1424 174 1396
Fragments arranged by size:
1506 1424 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2629 794
Size: 1835 3848
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 2803 1184
Size: 1619 4064
BstXI CCAn_nnnn'nTGG
Cuts at: 2974 2974
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678
Size: 1041 367 385 17 1452 86 12 346
Cuts at: 5678 1972
Size: 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2331 513
Size: 552 1266 3865
Fragments arranged by size:
3865 1266 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2143 2192 2641 3199 3993 4013 2143
Size: 49 449 558 794 20 3813
Fragments arranged by size:
3813 794 558 449 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3130 4019
Size: 54 1425 50 275 18 268 1032 889
Cuts at: 4019 5648 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1032 889 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 2679 3613 1353
Size: 1011 315 934 3423
Fragments arranged by size:
3423 1011 934 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2480 4270 4644 4790 387
Size: 1064 138 891 1790 374 146 1280
Fragments arranged by size:
1790 1280 1064 891 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2388 2501 2564 2888 3746 4343
Size: 393 436 320 113 63 324 858 597
Cuts at: 4343 5130 5398 1239
Size: 787 268 1524
Fragments arranged by size:
1524 858 787 597 436 393 324 320
268 113 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2130 2504 2684 2870 2130
Size: 374 180 186 4943
Fragments arranged by size:
4943 374 186 180
EcoO109I rG'GnC_Cy
Cuts at: 2179 2179
Size: 5683
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 2067 2633 4189 2067
Size: 566 1556 3561
Fragments arranged by size:
3561 1556 566
HaeII r_GCGC'y
Cuts at: 1678 2599 2658 2734 4830 1678
Size: 921 59 76 2096 2531
Fragments arranged by size:
2531 2096 921 76 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2345 2377 2477
Size: 32 135 32 452 32 289 32 100
Cuts at: 2477 2509 2585 2617 2681 2713 2861 2893 2930
Size: 32 76 32 64 32 148 32 37
Cuts at: 2930 2962 4324 4356 4398 4430 1373
Size: 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1362 452 289 148 135 100 76
64 42 37 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 4797 4981 1305
Size: 942 2550 184 2007
Fragments arranged by size:
2550 2007 942 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2330 2555 2801 3641 4000 4159
Size: 648 1050 114 225 246 840 359 159
Cuts at: 4159 518
Size: 2042
Fragments arranged by size:
2042 1050 840 648 359 246 225 159
114
MspA1I CmG'CkG
Cuts at: 1606 2055 2088 2359 2934 3099 3776 4924 5169
Size: 449 33 271 575 165 677 1148 245
Cuts at: 5169 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 575 449 271 245 165
33
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5131
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 3041 4586 1132
Size: 97 1812 1545 2229
Fragments arranged by size:
2229 1812 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2615 1348
Size: 1267 4416
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2303 2615 1108
Size: 1195 312 4176
Fragments arranged by size:
4176 1195 312
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2179
Size: 5683
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2187 2419 2463 2505 2526 2625 3035 830
Size: 1357 232 44 42 21 99 410 3478
Fragments arranged by size:
3478 1357 410 232 99 44 42 21
PvuI CG_AT'CG
Cuts at: 2001 2603 2001
Size: 602 5081
PvuII CAG'CTG
Cuts at: 2359 2359
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2183 2248 2415 2459 2501 2522 2621
Size: 219 1138 65 167 44 42 21 99
Cuts at: 2621 2658 3031 4166 4847 5038 826
Size: 37 373 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 373 219 191 167
99 65 44 42 37 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 2023 2045 3010 1872
Size: 151 22 965 4545
Fragments arranged by size:
4545 965 151 22
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2499 1517
Size: 982 4701
StyI C'CwwG_G
Cuts at: 513 1065 2907 3122 513
Size: 552 1842 215 3074
Fragments arranged by size:
3074 1842 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3710 3727 3880 4065 4510 325
Size: 1255 2130 17 153 185 445 1498
Fragments arranged by size:
2130 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 2171 2203 5643 5675 2060
Size: 32 79 32 3440 32 2068
Fragments arranged by size:
3440 2068 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 2021 2043 3008 1870
Size: 151 22 965 4545
Fragments arranged by size:
4545 965 151 22
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI