Restriction map for
pFN21AB7987
(Circular) (Six-base) MAPSORT of: pFN21AB7987.seq Check: 8580 from: 1 to: 6028
pFN21AB7987 6028 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3371 1937
Size: 1434 4594
Acc65I G'GTAC_C
Cuts at: 3147 3349 3147
Size: 202 5826
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2861 2878 2936 3065 4091 5475 5743
Size: 527 702 17 58 129 1026 1384 268
Cuts at: 5743 1632
Size: 1917
Fragments arranged by size:
1917 1384 1026 702 527 268 129 58
17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 4927 5904 1203
Size: 3724 977 1327
Fragments arranged by size:
3724 1327 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 2173 2326 4757 2173
Size: 153 2431 3444
Fragments arranged by size:
3444 2431 153
AleI CACnn'nnGTG
Cuts at: 1166 3315 1166
Size: 2149 3879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 2452 2680 2697 2839 3194 3771 5343 2452
Size: 228 17 142 355 577 1572 3137
Fragments arranged by size:
3137 1572 577 355 228 142 17
ApaI G_GGCC'C
Cuts at: 2073 2224 2912 2073
Size: 151 688 5189
Fragments arranged by size:
5189 688 151
ApaLI G'TGCA_C
Cuts at: 2876 3113 4085 5241 2876
Size: 237 972 1156 3663
Fragments arranged by size:
3663 1156 972 237
ApoI r'AATT_y
Cuts at: 1493 3337 3563 3715 5824 1493
Size: 1844 226 152 2109 1697
Fragments arranged by size:
2109 1844 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 2799 3293 3353 1870
Size: 73 856 494 60 4545
Fragments arranged by size:
4545 856 494 73 60
BamHI G'GATC_C
Cuts at: 2517 2853 3030 3358 3725 2517
Size: 336 177 328 367 4820
Fragments arranged by size:
4820 367 336 328 177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3147 3349 4804 618
Size: 325 217 627 1360 202 1455 1842
Fragments arranged by size:
1842 1455 1360 627 325 217 202
BanII G_rGCy'C
Cuts at: 1391 2073 2224 2912 3126 3347 1391
Size: 682 151 688 214 221 4072
Fragments arranged by size:
4072 688 682 221 214 151
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 1903
Size: 34 2282 34 3678
Fragments arranged by size:
3678 2282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 4639 136
Size: 107 122 71 631 605 484 2483 1525
Fragments arranged by size:
2483 1525 631 605 484 122 107 71
BglII A'GATC_T
Cuts at: 6023 6023
Size: 6028
BlpI GC'TnA_GC
Cuts at: 3445 3445
Size: 6028
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2224 2367 2465 2880 2912 3117 4089
Size: 283 151 143 98 415 32 205 972
Cuts at: 4089 5245 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 972 415 283 205 151 143
98 32
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 5200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3097 3759 4717
Size: 416 53 83 399 24 1680 662 958
Cuts at: 4717 442
Size: 1753
Fragments arranged by size:
1753 1680 958 662 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 4688 1239
Size: 1043 2406 2579
Fragments arranged by size:
2579 2406 1043
Bpu10I CC'TnA_GC
Cuts at: 3120 3120
Size: 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4153 5018 5316 4153
Size: 865 298 4865
Fragments arranged by size:
4865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 3300 4691 882
Size: 617 1023 778 1391 2219
Fragments arranged by size:
2219 1391 1023 778 617
BsaAI yAC'GTr
Cuts at: 493 1204 3819 493
Size: 711 2615 2702
Fragments arranged by size:
2702 2615 711
BsaBI GATnn'nnATC
Cuts at: 2132 6022 2132
Size: 3890 2138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2372 2501 3835
Size: 53 83 186 936 513 326 129 1334
Cuts at: 3835 4217 4852 275
Size: 382 635 1451
Fragments arranged by size:
1451 1334 936 635 513 382 326 186
129 83 53
BsaWI w'CCGG_w
Cuts at: 2292 4461 4859 5133 5280 5691 2292
Size: 2169 398 274 147 411 2629
Fragments arranged by size:
2629 2169 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5803
Fragments arranged by size:
5803 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 3005 1186
Size: 1210 609 4209
Fragments arranged by size:
4209 1210 609
BseYI C'CCAG_C
Cuts at: 2647 3187 3212 5231 5727 2647
Size: 540 25 2019 496 2948
Fragments arranged by size:
2948 2019 540 496 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 2101 2426 2693 2923 1163
Size: 573 324 41 325 267 230 4268
Fragments arranged by size:
4268 573 325 324 267 230 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2334 2391 3478 4239 5267 1540
Size: 275 186 333 57 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 333 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2607 2730 2880 3117 3126 4089 4174 5245 2607
Size: 123 150 237 9 963 85 1071 3390
Fragments arranged by size:
3390 1071 963 237 150 123 85 9
BsmI GAATG_Cn'
Cuts at: 3539 3632 3539
Size: 93 5935
BsmBI CGTCTCn'nnnn_
Cuts at: 2378 2378
Size: 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2224 2367 2465 2607 2730 2880
Size: 399 283 151 143 98 142 123 150
Cuts at: 2880 2912 3117 3126 3347 4089 4174 5245 1391
Size: 32 205 9 221 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 221 205 151
150 143 142 123 98 85 32 9
BspEI T'CCGG_A
Cuts at: 2292 5691 2292
Size: 3399 2629
BspHI T'CATG_A
Cuts at: 2061 3917 5647 2061
Size: 1856 1730 2442
Fragments arranged by size:
2442 1856 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 3135 3383 844
Size: 1844 447 248 3489
Fragments arranged by size:
3489 1844 447 248
BsrBI CCG'CTC
Cuts at: 1394 1823 2641 3915 1394
Size: 429 818 1274 3507
Fragments arranged by size:
3507 1274 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2096 2346 4523 4697 65
Size: 1183 848 250 2177 174 1396
Fragments arranged by size:
2177 1396 1183 848 250 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2424 4672 4859 1700
Size: 110 614 2248 187 2869
Fragments arranged by size:
2869 2248 614 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 2466 2879 4088 5100 2466
Size: 413 1209 1012 3394
Fragments arranged by size:
3394 1209 1012 413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2680 794
Size: 1886 4142
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6028
BstYI r'GATC_y
Cuts at: 1972 2187 2487 2517 2853 3006 3030 3358 3725
Size: 215 300 30 336 153 24 328 367
Cuts at: 3725 4110 4127 5579 5665 5677 6023 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 336 328 300
215 153 86 30 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2081 2367 2521 2857 3232 513
Size: 552 939 77 286 154 336 375 3309
Fragments arranged by size:
3309 939 552 375 336 286 154 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 2308 2574 2710 3544 4338 4358 2308
Size: 266 136 834 794 20 3978
Fragments arranged by size:
3978 834 794 266 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5993
DraI TTT'AAA
Cuts at: 3332 3332
Size: 6028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2756 3107 5035 809
Size: 507 1440 351 1928 1802
Fragments arranged by size:
1928 1802 1440 507 351
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2148 2331
Size: 54 1425 50 275 18 277 41 183
Cuts at: 2331 2388 3277 3475 4364 5993 8
Size: 57 889 198 889 1629 43
Fragments arranged by size:
1629 1425 889 889 277 275 198 183
57 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2331 2388 3475 1537
Size: 275 519 57 1087 4090
Fragments arranged by size:
4090 1087 519 275 57
EarI CTCTTCn'nnn_
Cuts at: 1353 2612 2690 3958 1353
Size: 1259 78 1268 3423
Fragments arranged by size:
3423 1268 1259 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3314 4615 4989 5135 387
Size: 1064 138 1725 1301 374 146 1280
Fragments arranged by size:
1725 1301 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3124 3124
Size: 6028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2282 2861 2878 2936 3065 4091
Size: 393 527 123 579 17 58 129 1026
Cuts at: 4091 4688 5475 5743 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1026 787 597 579 527 393 268
129 123 58 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2207 2843 3142 2207
Size: 636 299 5093
Fragments arranged by size:
5093 636 299
EcoO109I rG'GnC_Cy
Cuts at: 2032 2070 2200 2220 2221 2257 2677 2778 2836
Size: 38 130 20 1 36 420 101 58
Cuts at: 2836 2908 2909 2032
Size: 72 1 5151
Fragments arranged by size:
5151 420 130 101 72 58 38 36
20 1 1
EcoRI G'AATT_C
Cuts at: 3337 3715 3337
Size: 378 5650
FspI TGC'GCA
Cuts at: 4534 4534
Size: 6028
HaeII r_GCGC'y
Cuts at: 1678 2453 2511 2576 2648 2828 3219 5175 1678
Size: 775 58 65 72 180 391 1956 2531
Fragments arranged by size:
2531 1956 775 391 180 72 65 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2075 2159 2191 2312
Size: 32 135 32 471 32 84 32 121
Cuts at: 2312 2344 2486 2518 2957 2989 4669 4701 4743
Size: 32 142 32 439 32 1680 32 42
Cuts at: 4743 4775 1373
Size: 32 2626
Fragments arranged by size:
2626 1680 471 439 142 135 121 84
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3058 3372 3618 669
Size: 988 281 1120 314 246 3079
Fragments arranged by size:
3079 1120 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6028
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KpnI G_GTAC'C
Cuts at: 3151 3353 3151
Size: 202 5826
MfeI C'AATT_G
Cuts at: 3627 3627
Size: 6028
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2074 2607 2865 5142 5326 1305
Size: 769 533 258 2277 184 2007
Fragments arranged by size:
2277 2007 769 533 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 3279 10
Size: 54 1425 343 318 1129 2759
Fragments arranged by size:
2759 1425 1129 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3315 3986 4345 4504 518
Size: 648 1815 334 671 359 159 2042
Fragments arranged by size:
2042 1815 671 648 359 334 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2369 2523 2859 3191 3444 4121 5269
Size: 477 286 154 336 332 253 677 1148
Cuts at: 5269 5514 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 477 336 332 286 253
245 154
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4537
Fragments arranged by size:
4537 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6028
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 3386 4931 1132
Size: 97 2157 1545 2229
Fragments arranged by size:
2229 2157 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6028
PciI A'CATG_T
Cuts at: 4927 4927
Size: 6028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3087 1108
Size: 1979 4049
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4129 5610 5642 4097
Size: 32 1481 32 4483
Fragments arranged by size:
4483 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2257 2677 2200
Size: 57 420 5551
Fragments arranged by size:
5551 420 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 3598 3598
Size: 6028
PspOMI G'GGCC_C
Cuts at: 2069 2220 2908 2069
Size: 151 688 5189
Fragments arranged by size:
5189 688 151
PstI C_TGCA'G
Cuts at: 830 2658 2676 3380 830
Size: 1828 18 704 3478
Fragments arranged by size:
3478 1828 704 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
PvuII CAG'CTG
Cuts at: 3191 3191
Size: 6028
SacI G_AGCT'C
Cuts at: 3126 3126
Size: 6028
SacII CC_GC'GG
Cuts at: 2084 2370 2524 2860 2084
Size: 286 154 336 5252
Fragments arranged by size:
5252 336 286 154
SalI G'TCGA_C
Cuts at: 1936 3370 1936
Size: 1434 4594
SapI GCTCTTCn'nnn_
Cuts at: 2612 2690 2612
Size: 78 5950
SbfI CC_TGCA'GG
Cuts at: 2676 3380 2676
Size: 704 5324
ScaI AGT'ACT
Cuts at: 4276 5988 4276
Size: 1712 4316
SfcI C'TryA_G
Cuts at: 826 1045 2654 2672 3139 3376 4511 5192 5383
Size: 219 1609 18 467 237 1135 681 191
Cuts at: 5383 826
Size: 1471
Fragments arranged by size:
1609 1471 1135 681 467 237 219 191
18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2156 2156
Size: 6028
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6028
SmaI CCC'GGG
Cuts at: 1872 2801 3355 1872
Size: 929 554 4545
Fragments arranged by size:
4545 929 554
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4132 5033 5295 820
Size: 197 926 2189 901 262 1553
Fragments arranged by size:
2189 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3386 1132
Size: 2254 3774
SspI AAT'ATT
Cuts at: 5 52 3952 5
Size: 47 3900 2081
Fragments arranged by size:
3900 2081 47
StuI AGG'CCT
Cuts at: 1517 2588 1517
Size: 1071 4957
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 2583 3467 513
Size: 552 939 528 51 884 3074
Fragments arranged by size:
3074 939 884 552 528 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2941 3098 4055 4072 4225 4410 4855
Size: 1255 1361 157 957 17 153 185 445
Cuts at: 4855 325
Size: 1498
Fragments arranged by size:
1498 1361 1255 957 445 185 157 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4274 5986 96
Size: 274 80 33 51 1443 2297 1712 138
Fragments arranged by size:
2297 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2287 2319 2991 3023 5988 6020 2287
Size: 32 672 32 2965 32 2295
Fragments arranged by size:
2965 2295 672 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2550 1316
Size: 1234 4794
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 3322 2257
Size: 1065 4963
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6028
XmaI C'CCGG_G
Cuts at: 1870 2799 3353 1870
Size: 929 554 4545
Fragments arranged by size:
4545 929 554
XmnI GAAnn'nnTTC
Cuts at: 4157 4157
Size: 6028
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI
SexAI SfoI SrfI SwaI