Restriction map for
pFN21AB7984
(Circular) (Six-base) MAPSORT of: pFN21AB7984.seq Check: 9954 from: 1 to: 5677
pFN21AB7984 5677 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 5124 5392 1632
Size: 2108 1384 268 1917
Fragments arranged by size:
2108 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2358 2532 2850 2852 4576 5553 1203
Size: 1155 174 318 2 1724 977 1327
Fragments arranged by size:
1724 1327 1155 977 318 174 2
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 5645
AlwNI CAG_nnn'CTG
Cuts at: 2323 3420 4992 2323
Size: 1097 1572 3008
Fragments arranged by size:
3008 1572 1097
ApaLI G'TGCA_C
Cuts at: 2171 3734 4890 2171
Size: 1563 1156 2958
Fragments arranged by size:
2958 1563 1156
ApoI r'AATT_y
Cuts at: 1493 2785 2986 3212 3364 5473 1493
Size: 1292 201 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 201 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2406 2728 2860 3002 1870
Size: 73 463 322 132 142 4545
Fragments arranged by size:
4545 463 322 142 132 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2704 2794 2998 4453
Size: 325 217 627 462 455 90 204 1455
Cuts at: 4453 618
Size: 1842
Fragments arranged by size:
1842 1455 627 462 455 325 217 204
90
BanII G_rGCy'C
Cuts at: 1391 2514 2735 2996 1391
Size: 1123 221 261 4072
Fragments arranged by size:
4072 1123 261 221
BbeI G_GCGC'C
Cuts at: 2253 2253
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BbvCI CC'TCA_GC
Cuts at: 2710 2710
Size: 5677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 2175 2709 3738 4894 1790
Size: 385 534 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 534 385
BmgBI CAC'GTC
Cuts at: 1318 2245 1318
Size: 927 4750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2569 3408 4366
Size: 416 53 83 399 24 1152 839 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1152 958 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2186 2300 2345 2719 2884 4337 1239
Size: 937 10 114 45 374 165 1453 2579
Fragments arranged by size:
2579 1453 937 374 165 114 45 10
Bpu10I CC'TnA_GC
Cuts at: 2710 2710
Size: 5677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2853 3468 493
Size: 711 1649 615 2702
Fragments arranged by size:
2702 1649 711 615
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2724 2801 3484
Size: 53 83 186 936 717 474 77 683
Cuts at: 3484 3866 4501 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 717 683 635 474 382 186
83 77 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 2918 2948 1178
Size: 30 165 30 762 30 723 30 3907
Fragments arranged by size:
3907 762 723 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 2140 2820 1186
Size: 876 78 680 4043
Fragments arranged by size:
4043 876 680 78
BseYI C'CCAG_C
Cuts at: 2094 4880 5376 2094
Size: 2786 496 2395
Fragments arranged by size:
2786 2395 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2942 1163
Size: 573 1206 3898
Fragments arranged by size:
3898 1206 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 2141 3127 3888 4916 1540
Size: 275 186 66 74 986 761 1028 2301
Fragments arranged by size:
2301 1028 986 761 275 186 74 66
BsiHKAI G_wGCw'C
Cuts at: 2040 2175 3738 3823 4894 2040
Size: 135 1563 85 1071 2823
Fragments arranged by size:
2823 1563 1071 135 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
BsmBI CGTCTCn'nnnn_
Cuts at: 2074 2074
Size: 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2175 2514 2709 2735 2996 3738
Size: 399 250 135 339 195 26 261 742
Cuts at: 3738 3823 4894 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 261 250 195
135 85 26
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2967 4321 4508 1700
Size: 110 1157 1354 187 2869
Fragments arranged by size:
2869 1354 1157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2106 2790 2973 513
Size: 552 1041 684 183 3217
Fragments arranged by size:
3217 1041 684 552 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2721 2967 3193 3987 4007 2721
Size: 246 226 794 20 4391
Fragments arranged by size:
4391 794 246 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2059 4684 809
Size: 507 743 2625 1802
Fragments arranged by size:
2625 1802 743 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2868 3124
Size: 54 1425 50 275 18 234 804 256
Cuts at: 3124 4013 5642 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 804 275 256 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2064 3124 1537
Size: 275 252 1060 4090
Fragments arranged by size:
4090 1060 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 3607 1353
Size: 2254 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2572 4264 4638 4784 387
Size: 1064 138 983 1692 374 146 1280
Fragments arranged by size:
1692 1280 1064 983 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2186 2300 2345 2719 2884 3740
Size: 393 544 10 114 45 374 165 856
Cuts at: 3740 4337 5124 5392 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 856 787 597 544 393 374 268
165 114 45 10
EcoO109I rG'GnC_Cy
Cuts at: 2257 2572 2257
Size: 315 5362
EcoRI G'AATT_C
Cuts at: 2785 2986 3364 2785
Size: 201 378 5098
Fragments arranged by size:
5098 378 201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2042 2506 2042
Size: 464 5213
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2253 4824 1678
Size: 575 2571 2531
Fragments arranged by size:
2571 2531 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 4318 4350 4392
Size: 32 135 32 674 32 2040 32 42
Cuts at: 4392 4424 1373
Size: 32 2626
Fragments arranged by size:
2626 2040 674 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2632 3021 3267 669
Size: 988 281 694 389 246 3079
Fragments arranged by size:
3079 988 694 389 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2249 2249
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 2961 4791 4975 1305
Size: 903 753 1830 184 2007
Fragments arranged by size:
2007 1830 903 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2870 10
Size: 54 1425 343 1038 2817
Fragments arranged by size:
2817 1425 1038 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 3635 3994 4153 518
Size: 648 1521 948 359 159 2042
Fragments arranged by size:
2042 1521 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2290 2792 3093 3770 4918 5163 1606
Size: 442 242 502 301 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 502 442 301 245 242
NarI GG'CG_CC
Cuts at: 2250 2250
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 2106 513
Size: 552 1041 4084
Fragments arranged by size:
4084 1041 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2362 2536 2656 2692 3035 4580 1132
Size: 97 1133 174 120 36 343 1545 2229
Fragments arranged by size:
2229 1545 1133 343 174 120 97 36
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 2358 2532 4576 2358
Size: 174 2044 3459
Fragments arranged by size:
3459 2044 174
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 4462
PfoI T'CCnGG_A
Cuts at: 2316 2316
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PmlI CAC'GTG
Cuts at: 2853 2853
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 2950 3746 3778 5259 5291 2918
Size: 32 796 32 1481 32 3304
Fragments arranged by size:
3304 1481 796 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2257 2257
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 2723 1613
Size: 1110 4567
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspXI vC'TCGA_Gb
Cuts at: 2860 2860
Size: 5677
PstI C_TGCA'G
Cuts at: 830 3029 830
Size: 2199 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
SacII CC_GC'GG
Cuts at: 2793 2793
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 3025 4160 4841 5032 826
Size: 219 1980 1135 681 191 1471
Fragments arranged by size:
1980 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2251 2251
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 2730 3004 1872
Size: 858 274 4545
Fragments arranged by size:
4545 858 274
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2860 3781 4682 4944 820
Size: 197 926 917 921 901 262 1553
Fragments arranged by size:
1553 926 921 917 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2106 2568 3116 513
Size: 552 1041 462 548 3074
Fragments arranged by size:
3074 1041 552 548 462
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2680 3704 3721 3874 4059 4504 325
Size: 1255 1100 1024 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1100 1024 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2165 2197 5637 5669 2165
Size: 32 3440 32 2173
Fragments arranged by size:
3440 2173 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2161
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 2860 1943
Size: 917 4760
XmaI C'CCGG_G
Cuts at: 1870 2728 3002 1870
Size: 858 274 4545
Fragments arranged by size:
4545 858 274
XmnI GAAnn'nnTTC
Cuts at: 2489 3806 2489
Size: 1317 4360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI