Restriction map for
pFN21AB7975
(Circular) (Six-base) MAPSORT of: pFN21AB7975.seq Check: 7082 from: 1 to: 5986
pFN21AB7975 5986 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3212 3212
Size: 5986
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 2573 3184 4049 5433 5701 1632
Size: 866 75 611 865 1384 268 1917
Fragments arranged by size:
1917 1384 866 865 611 268 75
AfeI AGC'GCT
Cuts at: 2426 3041 2426
Size: 615 5371
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 2143 2353 4715 2143
Size: 210 2362 3414
Fragments arranged by size:
3414 2362 210
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 2781 3175 3729 5301 2781
Size: 394 554 1572 3466
Fragments arranged by size:
3466 1572 554 394
ApaLI G'TGCA_C
Cuts at: 3017 4043 5199 3017
Size: 1026 1156 3804
Fragments arranged by size:
3804 1156 1026
ApoI r'AATT_y
Cuts at: 1493 3295 3521 3673 5782 1493
Size: 1802 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3311 1870
Size: 73 1368 4545
Fragments arranged by size:
4545 1368 73
BamHI G'GATC_C
Cuts at: 2770 3316 3683 2770
Size: 546 367 5073
Fragments arranged by size:
5073 546 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 3140 3233 3241 3307
Size: 325 217 627 1199 154 93 8 66
Cuts at: 3307 4762 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217 154 93
66 8
BanII G_rGCy'C
Cuts at: 1391 3305 1391
Size: 1914 4072
BbeI G_GCGC'C
Cuts at: 2990 3237 2990
Size: 247 5739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2482 3878 5088 2482
Size: 1396 1210 3380
Fragments arranged by size:
3380 1396 1210
BclI T'GATC_A
Cuts at: 1548 2847 1548
Size: 1299 4687
BfrBI ATG'CAT
Cuts at: 2878 2878
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3176 3403 3176
Size: 227 5759
Bme1580I G_kGCm'C
Cuts at: 1790 2729 2963 2982 3021 3246 4047 5203 1790
Size: 939 234 19 39 225 801 1156 2573
Fragments arranged by size:
2573 1156 939 801 234 225 39 19
BmgBI CAC'GTC
Cuts at: 1318 2977 1318
Size: 1659 4327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2769 3085 3163
Size: 416 53 83 399 24 1352 316 78
Cuts at: 3163 3240 3717 4675 442
Size: 77 477 958 1753
Fragments arranged by size:
1753 1352 958 477 416 399 316 83
78 77 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1997 2816 2855 2966 3029 4646 1239
Size: 758 819 39 111 63 1617 2579
Fragments arranged by size:
2579 1617 819 758 111 63 39
Bpu10I CC'TnA_GC
Cuts at: 2675 2675
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 4111 4976 5274 2195
Size: 1916 865 298 2907
Fragments arranged by size:
2907 1916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2657 4649 882
Size: 617 1158 1992 2219
Fragments arranged by size:
2219 1992 1158 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2987 3234 3793 4175
Size: 53 83 186 936 1454 247 559 382
Cuts at: 4175 4810 275
Size: 635 1451
Fragments arranged by size:
1454 1451 936 635 559 382 247 186
83 53
BsaWI w'CCGG_w
Cuts at: 2139 2367 2886 4419 4817 5091 5238 5649 2139
Size: 228 519 1533 398 274 147 411 2476
Fragments arranged by size:
2476 1533 519 411 398 274 228 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3143 3173 1178
Size: 30 165 30 1740 30 3991
Fragments arranged by size:
3991 1740 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 3128 1186
Size: 1057 885 4044
Fragments arranged by size:
4044 1057 885
BseYI C'CCAG_C
Cuts at: 2955 5189 5685 2955
Size: 2234 496 3256
Fragments arranged by size:
3256 2234 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2158 2173 3436 4197 5225 1540
Size: 275 186 157 15 1263 761 1028 2301
Fragments arranged by size:
2301 1263 1028 761 275 186 157 15
BsiHKAI G_wGCw'C
Cuts at: 3021 3120 3182 4047 4132 5203 3021
Size: 99 62 865 85 1071 3804
Fragments arranged by size:
3804 1071 865 99 85 62
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
BsmBI CGTCTCn'nnnn_
Cuts at: 2678 2678
Size: 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2729 2963 2982 3021 3120 3182 3246
Size: 399 939 234 19 39 99 62 64
Cuts at: 3246 3305 4047 4132 5203 1391
Size: 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 939 742 399 234 99 85
64 62 59 39 19
BspEI T'CCGG_A
Cuts at: 2886 5649 2886
Size: 2763 3223
BspHI T'CATG_A
Cuts at: 2871 3875 5605 2871
Size: 1004 1730 3252
Fragments arranged by size:
3252 1730 1004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2622 2686 3212 3341 844
Size: 1778 64 526 129 3489
Fragments arranged by size:
3489 1778 526 129 64
BsrBI CCG'CTC
Cuts at: 1394 1823 2217 2233 3148 3212 3873 1394
Size: 429 394 16 915 64 661 3507
Fragments arranged by size:
3507 915 661 429 394 64 16
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2459 4630 4817 1700
Size: 110 649 2171 187 2869
Fragments arranged by size:
2869 2171 649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 2022 1925
Size: 97 5889
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 3044 3253 3044
Size: 209 5777
BstYI r'GATC_y
Cuts at: 1972 2770 2883 3316 3683 4068 4085 5537 5623
Size: 798 113 433 367 385 17 1452 86
Cuts at: 5623 5635 5981 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 798 433 385 367 346 113
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2296 3162 513
Size: 552 939 292 866 3337
Fragments arranged by size:
3337 939 866 552 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 3502 4296 4316 3268
Size: 234 794 20 4938
Fragments arranged by size:
4938 794 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2200 2235 529
Size: 35 1636 35 4280
Fragments arranged by size:
4280 1636 35 35
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2155 2461
Size: 54 1425 50 275 18 261 64 306
Cuts at: 2461 2910 3433 4322 5951 8
Size: 449 523 889 1629 43
Fragments arranged by size:
1629 1425 889 523 449 306 275 261
64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2155 3433 1537
Size: 275 343 1278 4090
Fragments arranged by size:
4090 1278 343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3916 1353
Size: 2563 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2347 4573 4947 5093 387
Size: 1064 138 758 2226 374 146 1280
Fragments arranged by size:
2226 1280 1064 758 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1997 2498 2573 2816 2855 2966 3029
Size: 393 365 501 75 243 39 111 63
Cuts at: 3029 3184 4049 4646 5433 5701 1239
Size: 155 865 597 787 268 1524
Fragments arranged by size:
1524 865 787 597 501 393 365 268
243 155 111 75 63 39
EcoO109I rG'GnC_Cy
Cuts at: 2145 2319 2601 2830 2145
Size: 174 282 229 5301
Fragments arranged by size:
5301 282 229 174
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FspI TGC'GCA
Cuts at: 2637 4492 2637
Size: 1855 4131
HaeII r_GCGC'y
Cuts at: 1678 2428 2682 2990 3043 3237 5133 1678
Size: 750 254 308 53 194 1896 2531
Fragments arranged by size:
2531 1896 750 308 254 194 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2339 2371 3143
Size: 32 135 32 557 32 178 32 772
Cuts at: 3143 3175 4627 4659 4701 4733 1373
Size: 32 1452 32 42 32 2626
Fragments arranged by size:
2626 1452 772 557 178 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2701 3330 3576 669
Size: 988 281 763 629 246 3079
Fragments arranged by size:
3079 988 763 629 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KasI G'GCGC_C
Cuts at: 2986 3233 2986
Size: 247 5739
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 2337 3585 2337
Size: 1248 4738
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2912 10
Size: 54 1425 343 1080 3084
Fragments arranged by size:
3084 1425 1080 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3944 4303 4462 518
Size: 648 2778 359 159 2042
Fragments arranged by size:
2778 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2154 2955 3070 3402 4079 5227 5472 1606
Size: 548 801 115 332 677 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 548 332 245 115
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 5986
NarI GG'CG_CC
Cuts at: 2987 3234 2987
Size: 247 5739
NcoI C'CATG_G
Cuts at: 513 1065 2004 3162 513
Size: 552 939 1158 3337
Fragments arranged by size:
3337 1158 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NsiI A_TGCA'T
Cuts at: 2880 2880
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2130 2305 3344 4889 1132
Size: 97 901 175 1039 1545 2229
Fragments arranged by size:
2229 1545 1039 901 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PfoI T'CCnGG_A
Cuts at: 2793 3006 2793
Size: 213 5773
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2145 2319 2601 2145
Size: 174 282 5530
Fragments arranged by size:
5530 282 174
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2153 2694 3338 830
Size: 1323 541 644 3478
Fragments arranged by size:
3478 1323 644 541
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2955 2955
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2149 2690 3334 4469 5150 5341 826
Size: 219 1104 541 644 1135 681 191 1471
Fragments arranged by size:
1471 1135 1104 681 644 541 219 191
SfoI GGC'GCC
Cuts at: 2988 3235 2988
Size: 247 5739
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 4090 4991 5253 820
Size: 197 926 267 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 267 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 2130 2305 3344 1132
Size: 998 175 1039 3774
Fragments arranged by size:
3774 1039 998 175
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2004 2982 3162 3237 3425 513
Size: 552 939 978 180 75 188 3074
Fragments arranged by size:
3074 978 939 552 188 180 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4013 4030 4183 4368 4813 325
Size: 1255 2433 17 153 185 445 1498
Fragments arranged by size:
2433 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 3030 4232
Size: 274 80 33 51 1443 825 228 1202
Cuts at: 4232 5944 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1202 825 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 2067 2174 1316
Size: 751 107 5128
Fragments arranged by size:
5128 751 107
XbaI T'CTAG_A
Cuts at: 2503 3322 2503
Size: 819 5167
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI