Restriction map for
pFN21AB7968
(Circular) (Six-base) MAPSORT of: pFN21AB7968.seq Check: 6068 from: 1 to: 5926
pFN21AB7968 5926 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 2134 3047 3131 3989 5373 5641 1632
Size: 432 70 913 84 858 1384 268 1917
Fragments arranged by size:
1917 1384 913 858 432 268 84 70
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 3066 4825 5802 1203
Size: 1863 1759 977 1327
Fragments arranged by size:
1863 1759 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2951 1184
Size: 1767 4159
AlwNI CAG_nnn'CTG
Cuts at: 2129 2287 2476 3669 5241 2129
Size: 158 189 1193 1572 2814
Fragments arranged by size:
2814 1572 1193 189 158
ApaI G_GGCC'C
Cuts at: 2102 2102
Size: 5926
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3016 3235 3461 3613 5722 1493
Size: 1523 219 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 219 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 2316 3251 1870
Size: 73 291 82 935 4545
Fragments arranged by size:
4545 935 291 82 73
AvrII C'CTAG_G
Cuts at: 2501 2501
Size: 5926
BamHI G'GATC_C
Cuts at: 3126 3256 3623 3126
Size: 130 367 5429
Fragments arranged by size:
5429 367 130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3247 4702 618
Size: 325 217 627 1460 1455 1842
Fragments arranged by size:
1842 1460 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 2136 2525 2570 3188 3245 1391
Size: 711 34 389 45 618 57 4072
Fragments arranged by size:
4072 711 618 389 57 45 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2196 2230 4117 4151 1903
Size: 34 259 34 1887 34 3678
Fragments arranged by size:
3678 1887 259 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2509 5921 2509
Size: 3412 2514
BlpI GC'TnA_GC
Cuts at: 2564 2965 3343 2564
Size: 401 378 5147
Fragments arranged by size:
5147 401 378
Bme1580I G_kGCm'C
Cuts at: 1790 2102 3987 5143 1790
Size: 312 1885 1156 2573
Fragments arranged by size:
2573 1885 1156 312
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2790 2212
Size: 578 5348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2511 3156 4586 1239
Size: 1272 645 1430 2579
Fragments arranged by size:
2579 1430 1272 645
Bpu10I CC'TnA_GC
Cuts at: 2130 2130
Size: 5926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3052 4589 882
Size: 617 1553 1537 2219
Fragments arranged by size:
2219 1553 1537 617
BsaAI yAC'GTr
Cuts at: 493 1204 3012 3069 3717 493
Size: 711 1808 57 648 2702
Fragments arranged by size:
2702 1808 711 648 57
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3733 4115 4750 275
Size: 53 83 186 936 2200 382 635 1451
Fragments arranged by size:
2200 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2094 2585 1186
Size: 908 491 4527
Fragments arranged by size:
4527 908 491
BseYI C'CCAG_C
Cuts at: 2199 2621 5129 5625 2199
Size: 422 2508 496 2500
Fragments arranged by size:
2508 2500 496 422
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 2721 3101 1163
Size: 573 489 496 380 3988
Fragments arranged by size:
3988 573 496 489 380
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 2439 2525 3188 3987 4072 5143 2136
Size: 303 86 663 799 85 1071 2919
Fragments arranged by size:
2919 1071 799 663 303 86 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2136 2439 2525 2570 3188 3245
Size: 399 312 34 303 86 45 618 57
Cuts at: 3245 3987 4072 5143 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 618 399 312 303 86
85 57 45 34
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2782 3281 844
Size: 1938 499 3489
Fragments arranged by size:
3489 1938 499
BsrBI CCG'CTC
Cuts at: 1394 1823 2964 3813 1394
Size: 429 1141 849 3507
Fragments arranged by size:
3507 1141 849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2839 4421 4595 65
Size: 1183 1591 1582 174 1396
Fragments arranged by size:
1591 1582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2559 4570 4757 1700
Size: 110 749 2011 187 2869
Fragments arranged by size:
2869 2011 749 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 2363 3986 4998 2363
Size: 1623 1012 3291
Fragments arranged by size:
3291 1623 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 794
Size: 1640 4286
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 2948 1184
Size: 1764 4162
BstXI CCAn_nnnn'nTGG
Cuts at: 2403 2403
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2125 2307 2453 2509 2717 3126 3256 3623
Size: 153 182 146 56 208 409 130 367
Cuts at: 3623 4008 4025 5477 5563 5575 5921 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 409 385 367 346 208 182
153 146 130 86 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3217 513
Size: 552 939 1213 3222
Fragments arranged by size:
3222 1213 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 2709 3442 4236 4256 2408
Size: 301 733 794 20 4078
Fragments arranged by size:
4078 794 733 301 20
ClaI AT'CG_AT
Cuts at: 1554 2329 1554
Size: 775 5151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 3157 3373
Size: 54 1425 50 275 18 579 748 216
Cuts at: 3373 4262 5891 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 748 579 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 3023 3856 1353
Size: 729 941 833 3423
Fragments arranged by size:
3423 941 833 729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2134 2523 3186 2134
Size: 389 663 4874
Fragments arranged by size:
4874 663 389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2134 2511 3047 3131 3156 3989
Size: 393 432 70 377 536 84 25 833
Cuts at: 3989 4586 5373 5641 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 833 787 597 536 432 393 377
268 84 70 25
EcoO109I rG'GnC_Cy
Cuts at: 2098 2099 2632 2098
Size: 1 533 5392
Fragments arranged by size:
5392 533 1
EcoRI G'AATT_C
Cuts at: 3016 3235 3613 3016
Size: 219 378 5329
Fragments arranged by size:
5329 378 219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 5926
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2195 5073 1678
Size: 517 2878 2531
Fragments arranged by size:
2878 2531 517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2299 2331 2557 2589 4567
Size: 32 135 32 727 32 226 32 1978
Cuts at: 4567 4599 4641 4673 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1978 727 226 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3270 3516 669
Size: 988 281 1167 165 246 3079
Fragments arranged by size:
3079 1167 988 281 246 165
HindIII A'AGCT_T
Cuts at: 748 2046 3004 3172 748
Size: 1298 958 168 3502
Fragments arranged by size:
3502 1298 958 168
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2230 5040 5224 1305
Size: 756 169 2810 184 2007
Fragments arranged by size:
2810 2007 756 184 169
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3159 10
Size: 54 1425 343 1327 2777
Fragments arranged by size:
2777 1425 1327 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3151 3884 4243 4402 518
Size: 648 1985 733 359 159 2042
Fragments arranged by size:
2042 1985 733 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2711 3342 4019 5167 5412 1606
Size: 1105 631 677 1148 245 2120
Fragments arranged by size:
2120 1148 1105 677 631 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3217 513
Size: 552 939 1213 3222
Fragments arranged by size:
3222 1213 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3284 4829 1132
Size: 97 2055 1545 2229
Fragments arranged by size:
2229 2055 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PmlI CAC'GTG
Cuts at: 3012 3069 3012
Size: 57 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 2098 2098
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2739 3278 830
Size: 1909 539 3478
Fragments arranged by size:
3478 1909 539
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2711 2711
Size: 5926
SacI G_AGCT'C
Cuts at: 2136 2525 3188 2136
Size: 389 663 4874
Fragments arranged by size:
4874 663 389
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2735 3274 4409 5090 5281 826
Size: 219 1690 539 1135 681 191 1471
Fragments arranged by size:
1690 1471 1135 681 539 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2501 3217 3365 513
Size: 552 939 243 254 716 148 3074
Fragments arranged by size:
3074 939 716 552 254 243 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2395 2895 3953 3970 4123 4308 4753
Size: 1255 815 500 1058 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1498 1255 1058 815 500 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4172 5884 96
Size: 274 80 33 51 1443 2195 1712 138
Fragments arranged by size:
2195 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 5886 5918 2369
Size: 32 3485 32 2377
Fragments arranged by size:
3485 2377 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 2373 3005 4055 2373
Size: 632 1050 4244
Fragments arranged by size:
4244 1050 632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI