Restriction map for
pFN21AB7965
(Circular) (Six-base) MAPSORT of: pFN21AB7965.seq Check: 2416 from: 1 to: 5545
pFN21AB7965 5545 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2745 2888 1937
Size: 808 143 4594
Fragments arranged by size:
4594 808 143
Acc65I G'GTAC_C
Cuts at: 2668 2866 2668
Size: 198 5347
AclI AA'CG_TT
Cuts at: 1563 2844 3672 4045 1563
Size: 1281 828 373 3063
Fragments arranged by size:
3063 1281 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 2329 3608 4992 5260 1632
Size: 587 110 1279 1384 268 1917
Fragments arranged by size:
1917 1384 1279 587 268 110
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 4274 4274
Size: 5545
AleI CACnn'nnGTG
Cuts at: 1166 2768 1166
Size: 1602 3943
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 1184
Size: 1251 4294
AlwNI CAG_nnn'CTG
Cuts at: 2099 3288 4860 2099
Size: 1189 1572 2784
Fragments arranged by size:
2784 1572 1189
ApaI G_GGCC'C
Cuts at: 2777 2777
Size: 5545
ApaLI G'TGCA_C
Cuts at: 3602 4758 3602
Size: 1156 4389
ApoI r'AATT_y
Cuts at: 1493 2029 2854 3080 3232 5341 1493
Size: 536 825 226 152 2109 1697
Fragments arranged by size:
2109 1697 825 536 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 2576 2870 1870
Size: 73 528 105 294 4545
Fragments arranged by size:
4545 528 294 105 73
BamHI G'GATC_C
Cuts at: 2875 3242 2875
Size: 367 5178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2497 2587 2603 2668 2762
Size: 325 217 627 710 90 16 65 94
Cuts at: 2762 2866 4321 618
Size: 104 1455 1842
Fragments arranged by size:
1842 1455 710 627 325 217 104 94
90 65 16
BanII G_rGCy'C
Cuts at: 1391 2777 2864 1391
Size: 1386 87 4072
Fragments arranged by size:
4072 1386 87
BbeI G_GCGC'C
Cuts at: 2501 2591 2607 2501
Size: 90 16 5439
Fragments arranged by size:
5439 90 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3736 3770 1903
Size: 34 50 34 1715 34 3678
Fragments arranged by size:
3678 1715 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2495 4156 136
Size: 107 122 71 631 605 823 1661 1525
Fragments arranged by size:
1661 1525 823 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5540 5540
Size: 5545
BlpI GC'TnA_GC
Cuts at: 2303 2962 2303
Size: 659 4886
Bme1580I G_kGCm'C
Cuts at: 1790 2571 2777 3606 4762 1790
Size: 781 206 829 1156 2573
Fragments arranged by size:
2573 1156 829 781 206
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3276 4234 442
Size: 416 53 83 399 24 1859 958 1753
Fragments arranged by size:
1859 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2652 4205 1239
Size: 1010 403 1553 2579
Fragments arranged by size:
2579 1553 1010 403
Bpu10I CC'TnA_GC
Cuts at: 2643 2643
Size: 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 4535 4833 3670
Size: 865 298 4382
Fragments arranged by size:
4382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4208 882
Size: 617 2709 2219
Fragments arranged by size:
2709 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2385 3336 493
Size: 711 1181 951 2702
Fragments arranged by size:
2702 1181 951 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2498 2588 2604 3352
Size: 53 83 186 936 965 90 16 748
Cuts at: 3352 3734 4369 275
Size: 382 635 1451
Fragments arranged by size:
1451 965 936 748 635 382 186 90
83 53 16
BsaWI w'CCGG_w
Cuts at: 2077 2268 3978 4376 4650 4797 5208 2077
Size: 191 1710 398 274 147 411 2414
Fragments arranged by size:
2414 1710 411 398 274 191 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5320
Fragments arranged by size:
5320 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5545
BseYI C'CCAG_C
Cuts at: 2056 2323 2532 2617 4748 5244 2056
Size: 267 209 85 2131 496 2357
Fragments arranged by size:
2357 2131 496 267 209 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4972
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2995 3756 4784 1540
Size: 275 186 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2309 3606 3691 4762 2106
Size: 203 1297 85 1071 2889
Fragments arranged by size:
2889 1297 1071 203 85
BsmI GAATG_Cn'
Cuts at: 2218 3056 3149 2218
Size: 838 93 4614
Fragments arranged by size:
4614 838 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2309 2571 2777 2864 3606 3691
Size: 399 316 203 262 206 87 742 85
Cuts at: 3691 4762 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 316 262 206 203
87 85
BspEI T'CCGG_A
Cuts at: 2268 5208 2268
Size: 2940 2605
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 2900 844
Size: 1439 617 3489
Fragments arranged by size:
3489 1439 617
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 1394
Size: 429 1609 3507
Fragments arranged by size:
3507 1609 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4040 4214 65
Size: 1183 2792 174 1396
Fragments arranged by size:
2792 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2339 2671 4189 4376 1700
Size: 110 529 332 1518 187 2869
Fragments arranged by size:
2869 1518 529 332 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 2564 3605 4617 2564
Size: 1041 1012 3492
Fragments arranged by size:
3492 1041 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 2365 1184
Size: 1181 4364
BstYI r'GATC_y
Cuts at: 1972 2875 3242 3627 3644 5096 5182 5194 5540
Size: 903 367 385 17 1452 86 12 346
Cuts at: 5540 1972
Size: 1977
Fragments arranged by size:
1977 1452 903 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2769 2834 513
Size: 552 1704 65 3224
Fragments arranged by size:
3224 1704 552 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 2790 3061 3855 3875 2790
Size: 271 794 20 4460
Fragments arranged by size:
4460 794 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4552 809
Size: 507 3236 1802
Fragments arranged by size:
3236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2425 2433 2683
Size: 54 1425 50 275 18 595 8 250
Cuts at: 2683 2992 3881 5510 8
Size: 309 889 1629 43
Fragments arranged by size:
1629 1425 889 595 309 275 250 54
50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2137 3475 1353
Size: 784 1338 3423
Fragments arranged by size:
3423 1338 784
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2252 2831 2854 4132 4506 4652
Size: 1064 138 663 579 23 1278 374 146
Cuts at: 4652 387
Size: 1280
Fragments arranged by size:
1280 1278 1064 663 579 374 146 138
23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2249 2329 2652 3608 4205 4992
Size: 393 587 30 80 323 956 597 787
Cuts at: 4992 5260 1239
Size: 268 1524
Fragments arranged by size:
1524 956 787 597 587 393 323 268
80 30
EcoO109I rG'GnC_Cy
Cuts at: 2519 2712 2774 2519
Size: 193 62 5290
Fragments arranged by size:
5290 193 62
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
FspI TGC'GCA
Cuts at: 2411 4051 2411
Size: 1640 3905
FspAI rTGC'GCAy
Cuts at: 2411 2411
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 2501 2591 2607 4692 1678
Size: 823 90 16 2085 2531
Fragments arranged by size:
2531 2085 823 90 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373
Size: 32 135 32 2614 32 42 32 2626
Fragments arranged by size:
2626 2614 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 2889 3135 669
Size: 988 281 130 821 246 3079
Fragments arranged by size:
3079 988 821 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KasI G'GCGC_C
Cuts at: 2497 2587 2603 2497
Size: 90 16 5439
Fragments arranged by size:
5439 90 16
KpnI G_GTAC'C
Cuts at: 2672 2870 2672
Size: 198 5347
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4659 4843 1305
Size: 3354 184 2007
Fragments arranged by size:
3354 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 2685 10
Size: 54 1425 343 603 250 2870
Fragments arranged by size:
2870 1425 603 343 250 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2768 3503 3862 4021 518
Size: 648 1602 735 359 159 2042
Fragments arranged by size:
2042 1602 735 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2173 2281 2727 2760 2799 2961 3638
Size: 406 161 108 446 33 39 162 677
Cuts at: 3638 4786 5031 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 446 406 245 162 161
108 39 33
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 5545
NarI GG'CG_CC
Cuts at: 2498 2588 2604 2498
Size: 90 16 5439
Fragments arranged by size:
5439 90 16
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4993
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2903 4448 1132
Size: 97 1674 1545 2229
Fragments arranged by size:
2229 1674 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5545
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5545
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PmlI CAC'GTG
Cuts at: 2385 2385
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2519 2519
Size: 5545
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PspOMI G'GGCC_C
Cuts at: 2773 2773
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2280 2897 830
Size: 1450 617 3478
Fragments arranged by size:
3478 1450 617
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
PvuII CAG'CTG
Cuts at: 2012 2173 2012
Size: 161 5384
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SanDI GG'GwC_CC
Cuts at: 2519 2519
Size: 5545
SapI GCTCTTCn'nnn_
Cuts at: 2137 2137
Size: 5545
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SfcI C'TryA_G
Cuts at: 826 1045 2276 2893 4028 4709 4900 826
Size: 219 1231 617 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 681 617 219 191
SfoI GGC'GCC
Cuts at: 2499 2589 2605 2499
Size: 90 16 5439
Fragments arranged by size:
5439 90 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2473 2578 2872 1872
Size: 601 105 294 4545
Fragments arranged by size:
4545 601 294 105
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3649 4550 4812 820
Size: 197 926 1706 901 262 1553
Fragments arranged by size:
1706 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5545
StyI C'CwwG_G
Cuts at: 513 1065 2984 513
Size: 552 1919 3074
Fragments arranged by size:
3074 1919 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3572 3589 3742 3927 4372 325
Size: 1255 1992 17 153 185 445 1498
Fragments arranged by size:
1992 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3791 5503 96
Size: 274 80 33 51 1443 1814 1712 138
Fragments arranged by size:
1814 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2810 2842 5505 5537 2810
Size: 32 2663 32 2818
Fragments arranged by size:
2818 2663 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5545
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5545
XmaI C'CCGG_G
Cuts at: 1870 2471 2576 2870 1870
Size: 601 105 294 4545
Fragments arranged by size:
4545 601 294 105
XmnI GAAnn'nnTTC
Cuts at: 3674 3674
Size: 5545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI
PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI
SwaI XcmI