Restriction map for
pFN21AB7961
(Circular) (Six-base) MAPSORT of: pFN21AB7961.seq Check: 2899 from: 1 to: 5500
pFN21AB7961 5500 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 2583 3563 4947 5215 1632
Size: 801 150 980 1384 268 1917
Fragments arranged by size:
1917 1384 980 801 268 150
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 2779 1166
Size: 1613 3887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2081 2098 1184
Size: 897 17 4586
Fragments arranged by size:
4586 897 17
AlwNI CAG_nnn'CTG
Cuts at: 3243 4815 3243
Size: 1572 3928
ApaLI G'TGCA_C
Cuts at: 3557 4713 3557
Size: 1156 4344
ApoI r'AATT_y
Cuts at: 1493 2534 2607 2724 2759 2809 3035 3187 5296
Size: 1041 73 117 35 50 226 152 2109
Cuts at: 5296 1493
Size: 1697
Fragments arranged by size:
2109 1697 1041 226 152 117 73 50
35
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2825 1870
Size: 73 882 4545
Fragments arranged by size:
4545 882 73
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2821 4276 618
Size: 325 217 627 1034 1455 1842
Fragments arranged by size:
1842 1455 1034 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2701 2819 1391
Size: 1310 118 4072
Fragments arranged by size:
4072 1310 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2796 928
Size: 971 897 3632
Fragments arranged by size:
3632 971 897
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3691 3725 1903
Size: 34 50 34 1670 34 3678
Fragments arranged by size:
3678 1670 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BfrBI ATG'CAT
Cuts at: 2075 2075
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2346 4111 136
Size: 107 122 71 631 605 674 1765 1525
Fragments arranged by size:
1765 1525 674 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5495 5495
Size: 5500
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 3561 4717 1790
Size: 1771 1156 2573
Fragments arranged by size:
2573 1771 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4189 442
Size: 416 53 83 399 24 1814 958 1753
Fragments arranged by size:
1814 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2354 4160 1239
Size: 968 147 1806 2579
Fragments arranged by size:
2579 1806 968 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2538 4163 882
Size: 617 1039 1625 2219
Fragments arranged by size:
2219 1625 1039 617
BsaAI yAC'GTr
Cuts at: 493 1204 3291 493
Size: 711 2087 2702
Fragments arranged by size:
2702 2087 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3307 3689 4324 275
Size: 53 83 186 936 1774 382 635 1451
Fragments arranged by size:
1774 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5275
Fragments arranged by size:
5275 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 1186
Size: 1147 4353
BseYI C'CCAG_C
Cuts at: 4703 5199 4703
Size: 496 5004
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2323 1163
Size: 573 587 4340
Fragments arranged by size:
4340 587 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2950 3711 4739 1540
Size: 275 186 949 761 1028 2301
Fragments arranged by size:
2301 1028 949 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3561 3646 4717 3561
Size: 85 1071 4344
Fragments arranged by size:
4344 1071 85
BsmI GAATG_Cn'
Cuts at: 2577 3011 3104 2577
Size: 434 93 4973
Fragments arranged by size:
4973 434 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2701 2819 3561 3646 4717 1391
Size: 399 911 118 742 85 1071 2174
Fragments arranged by size:
2174 1071 911 742 399 118 85
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2556 2855 844
Size: 1712 299 3489
Fragments arranged by size:
3489 1712 299
BsrBI CCG'CTC
Cuts at: 1394 1823 2734 3387 1394
Size: 429 911 653 3507
Fragments arranged by size:
3507 911 653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2125 3995 4169 65
Size: 1183 877 1870 174 1396
Fragments arranged by size:
1870 1396 1183 877 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4144 4331 1700
Size: 110 2334 187 2869
Fragments arranged by size:
2869 2334 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 2842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5500
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstYI r'GATC_y
Cuts at: 1972 2830 3197 3582 3599 5051 5137 5149 5495
Size: 858 367 385 17 1452 86 12 346
Cuts at: 5495 1972
Size: 1977
Fragments arranged by size:
1977 1452 858 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4948
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3810 3830 3016
Size: 794 20 4686
Fragments arranged by size:
4686 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2269 2304 529
Size: 35 1705 35 3725
Fragments arranged by size:
3725 1705 35 35
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 809
Size: 507 3191 1802
Fragments arranged by size:
3191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2947 3836 5465
Size: 54 1425 50 275 18 1117 889 1629
Cuts at: 5465 8
Size: 43
Fragments arranged by size:
1629 1425 1117 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2947 1537
Size: 275 1135 4090
Fragments arranged by size:
4090 1135 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 3430 1353
Size: 765 1312 3423
Fragments arranged by size:
3423 1312 765
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4087 4461 4607 387
Size: 1064 138 2498 374 146 1280
Fragments arranged by size:
2498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2354 2433 2583 3563 4160 4947
Size: 393 575 147 79 150 980 597 787
Cuts at: 4947 5215 1239
Size: 268 1524
Fragments arranged by size:
1524 980 787 597 575 393 268 150
147 79
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2206 4647 1678
Size: 528 2441 2531
Fragments arranged by size:
2531 2441 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4141 4173 4215 4247 1373
Size: 32 135 32 2569 32 42 32 2626
Fragments arranged by size:
2626 2569 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 2844 3090 669
Size: 988 281 802 104 246 3079
Fragments arranged by size:
3079 988 802 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 2257 3099 2257
Size: 842 4658
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 2229 2475 4614 4798 1305
Size: 714 210 246 2139 184 2007
Fragments arranged by size:
2139 2007 714 246 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3678
Fragments arranged by size:
3678 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2779 3458 3817 3976 518
Size: 648 1613 679 359 159 2042
Fragments arranged by size:
2042 1613 679 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2336 2916 3593 4741 4986 1606
Size: 693 37 580 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 580 245 37
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4948
NdeI CA'TA_TG
Cuts at: 387 2680 387
Size: 2293 3207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NsiI A_TGCA'T
Cuts at: 2077 2077
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2858 4403 1132
Size: 97 1629 1545 2229
Fragments arranged by size:
2229 1629 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5500
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5500
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2177 1613
Size: 564 4936
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PstI C_TGCA'G
Cuts at: 830 2298 2852 830
Size: 1468 554 3478
Fragments arranged by size:
3478 1468 554
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
PvuII CAG'CTG
Cuts at: 2299 2336 2299
Size: 37 5463
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 2129 3748 5460 2129
Size: 1619 1712 2169
Fragments arranged by size:
2169 1712 1619
SfcI C'TryA_G
Cuts at: 826 1045 2294 2848 3983 4664 4855 826
Size: 219 1249 554 1135 681 191 1471
Fragments arranged by size:
1471 1249 1135 681 554 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2827 1872
Size: 955 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2858 1132
Size: 1726 3774
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2939 513
Size: 552 1874 3074
Fragments arranged by size:
3074 1874 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2757 3527 3544 3697 3882 4327 325
Size: 1255 1177 770 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1177 770 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 2241 2358
Size: 274 80 33 51 1443 150 114 117
Cuts at: 2358 2754 3746 5458 96
Size: 396 992 1712 138
Fragments arranged by size:
1712 1443 992 396 274 150 138 117
114 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5500
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2825 1870
Size: 955 4545
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI