Restriction map for
pFN21AB7959
(Circular) (Six-base) MAPSORT of: pFN21AB7959.seq Check: 9558 from: 1 to: 5497
pFN21AB7959 5497 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3560 4944 5212 1632
Size: 1928 1384 268 1917
Fragments arranged by size:
1928 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 2132 4396 5373 1203
Size: 929 2264 977 1327
Fragments arranged by size:
2264 1327 977 929
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 2124 2757 1166
Size: 958 633 3906
Fragments arranged by size:
3906 958 633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 2311 2482 2617 3240 4812 2311
Size: 171 135 623 1572 2996
Fragments arranged by size:
2996 1572 623 171 135
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2261 2300 2806 3032 3184 5293 1493
Size: 768 39 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 768 506 226 152 39
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 2822 1870
Size: 73 612 267 4545
Fragments arranged by size:
4545 612 267 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2684 2818 4273 618
Size: 325 217 627 897 134 1455 1842
Fragments arranged by size:
1842 1455 897 627 325 217 134
BanII G_rGCy'C
Cuts at: 1391 2237 2816 1391
Size: 846 579 4072
Fragments arranged by size:
4072 846 579
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2745 928
Size: 971 846 3680
Fragments arranged by size:
3680 971 846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2223 4108 136
Size: 107 122 71 631 605 551 1885 1525
Fragments arranged by size:
1885 1525 631 605 551 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2078 2914 2078
Size: 836 4661
Bme1580I G_kGCm'C
Cuts at: 1790 2476 2754 3558 4714 1790
Size: 686 278 804 1156 2573
Fragments arranged by size:
2573 1156 804 686 278
BmgBI CAC'GTC
Cuts at: 1318 2122 1318
Size: 804 4693
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4186 442
Size: 416 53 83 399 24 1811 958 1753
Fragments arranged by size:
1811 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4157 1239
Size: 2918 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2318 3622 4487 4785 2318
Size: 1304 865 298 3030
Fragments arranged by size:
3030 1304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3304 3686 4321 275
Size: 53 83 186 936 1771 382 635 1451
Fragments arranged by size:
1771 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2568 2598 1178
Size: 30 165 30 1165 30 4077
Fragments arranged by size:
4077 1165 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2227 2684 1186
Size: 1041 457 3999
Fragments arranged by size:
3999 1041 457
BseYI C'CCAG_C
Cuts at: 2095 2250 2604 2710 4700 5196 2095
Size: 155 354 106 1990 496 2396
Fragments arranged by size:
2396 1990 496 354 155 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4924
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2223 2287 2947 3708 4736 1540
Size: 275 186 222 64 660 761 1028 2301
Fragments arranged by size:
2301 1028 761 660 275 222 186 64
BsiHKAI G_wGCw'C
Cuts at: 2121 2505 3558 3643 4714 2121
Size: 384 1053 85 1071 2904
Fragments arranged by size:
2904 1071 1053 384 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2237 2476 2505 2754 2816 3558
Size: 399 331 116 239 29 249 62 742
Cuts at: 3558 3643 4714 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 249 239 116
85 62 29
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2503 2533 2852 844
Size: 1659 30 319 3489
Fragments arranged by size:
3489 1659 319 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2552 3384 1394
Size: 429 729 832 3507
Fragments arranged by size:
3507 832 729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2158 2222 2668 2687 4141 4328 1700
Size: 110 348 64 446 19 1454 187 2869
Fragments arranged by size:
2869 1454 446 348 187 110 64 19
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 2545 1184
Size: 1361 4136
BstYI r'GATC_y
Cuts at: 1972 2827 3194 3579 3596 5048 5134 5146 5492
Size: 855 367 385 17 1452 86 12 346
Cuts at: 5492 1972
Size: 1977
Fragments arranged by size:
1977 1452 855 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2028 2028
Size: 5497
BtgI C'CryG_G
Cuts at: 513 1065 2125 2643 513
Size: 552 1060 518 3367
Fragments arranged by size:
3367 1060 552 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3807 3827 3013
Size: 794 20 4683
Fragments arranged by size:
4683 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DraIII CAC_nnn'GTG
Cuts at: 2389 2389
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2355 2944
Size: 54 1425 50 275 18 390 135 589
Cuts at: 2944 3833 5462 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 589 390 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2220 2944 1537
Size: 275 408 724 4090
Fragments arranged by size:
4090 724 408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3560 4157 4944 5212 1239
Size: 393 1928 597 787 268 1524
Fragments arranged by size:
1928 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2031 2732 2031
Size: 701 4796
EcoO109I rG'GnC_Cy
Cuts at: 2184 2479 2614 2184
Size: 295 135 5067
Fragments arranged by size:
5067 295 135
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 5497
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 4644 1678
Size: 2966 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2094 2126 2365 2397 2568
Size: 32 135 32 522 32 239 32 171
Cuts at: 2568 2600 4138 4170 4212 4244 1373
Size: 32 1538 32 42 32 2626
Fragments arranged by size:
2626 1538 522 239 171 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 2132 5373 2132
Size: 3241 2256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2599 2772 4611 4795 1305
Size: 1294 173 1839 184 2007
Fragments arranged by size:
2007 1839 1294 184 173
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2357 10
Size: 54 1425 343 525 3150
Fragments arranged by size:
3150 1425 525 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2016 2124 2426 2757 3455 3814 3973
Size: 648 850 108 302 331 698 359 159
Cuts at: 3973 518
Size: 2042
Fragments arranged by size:
2042 850 698 648 359 331 302 159
108
MspA1I CmG'CkG
Cuts at: 1606 2048 2599 2608 2913 3590 4738 4983 1606
Size: 442 551 9 305 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 551 442 305 245 9
NaeI GCC'GGC
Cuts at: 2160 2224 2670 2160
Size: 64 446 4987
Fragments arranged by size:
4987 446 64
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4945
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NgoMIV G'CCGG_C
Cuts at: 2158 2222 2668 2158
Size: 64 446 4987
Fragments arranged by size:
4987 446 64
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2184 2479 2184
Size: 295 5202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2061 2613 2655 2849 830
Size: 1231 552 42 194 3478
Fragments arranged by size:
3478 1231 552 194 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
PvuII CAG'CTG
Cuts at: 2599 2608 2599
Size: 9 5488
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SanDI GG'GwC_CC
Cuts at: 2184 2184
Size: 5497
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2057 2609 2651 2845 3980 4661 4852
Size: 219 1012 552 42 194 1135 681 191
Cuts at: 4852 826
Size: 1471
Fragments arranged by size:
1471 1135 1012 681 552 219 194 191
42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 3601 4502 4764 820
Size: 197 926 390 1268 901 262 1553
Fragments arranged by size:
1553 1268 926 901 390 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2106 2733 2936 513
Size: 552 1041 627 203 3074
Fragments arranged by size:
3074 1041 627 552 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2780 3524 3541 3694 3879 4324 325
Size: 1255 1200 744 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 3743 5455
Size: 274 80 33 51 1443 417 1349 1712
Cuts at: 5455 96
Size: 138
Fragments arranged by size:
1712 1443 1349 417 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2585 2617 5457 5489 2585
Size: 32 2840 32 2593
Fragments arranged by size:
2840 2593 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2777 2777
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 2021 3626 2021
Size: 1605 3892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI