Restriction map for
pFN21AB7958
(Circular) (Six-base) MAPSORT of: pFN21AB7958.seq Check: 8332 from: 1 to: 5893
pFN21AB7958 5893 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 1937
Size: 1299 4594
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3956 5340 5608 1632
Size: 2324 1384 268 1917
Fragments arranged by size:
2324 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2993 820
Size: 197 1976 3720
Fragments arranged by size:
3720 1976 197
AflIII A'CryG_T
Cuts at: 1203 2178 2252 4792 5769 1203
Size: 975 74 2540 977 1327
Fragments arranged by size:
2540 1327 977 975 74
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 2524 4622 2524
Size: 2098 3795
AleI CACnn'nnGTG
Cuts at: 1166 2133 2846 1166
Size: 967 713 4213
Fragments arranged by size:
4213 967 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 3069 3113 3636 5208 3069
Size: 44 523 1572 3754
Fragments arranged by size:
3754 1572 523 44
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2891 2921 3037 3202 3428 3580 5689 1493
Size: 1398 30 116 165 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 226 165 152 116 30
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 3218 1870
Size: 73 1275 4545
Fragments arranged by size:
4545 1275 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2309 2342 2309
Size: 33 5860
BamHI G'GATC_C
Cuts at: 2531 3223 3590 2531
Size: 692 367 4834
Fragments arranged by size:
4834 692 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3214 4669 618
Size: 325 217 627 1427 1455 1842
Fragments arranged by size:
1842 1455 1427 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3212 1391
Size: 1821 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4084 4118 1903
Size: 34 50 34 2063 34 3678
Fragments arranged by size:
3678 2063 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2337 2380 3785 4995 2337
Size: 43 1405 1210 3235
Fragments arranged by size:
3235 1405 1210 43
BclI T'GATC_A
Cuts at: 1548 2683 1548
Size: 1135 4758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 3954 5110 1790
Size: 2164 1156 2573
Fragments arranged by size:
2573 2164 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4582 442
Size: 416 53 83 399 24 2207 958 1753
Fragments arranged by size:
2207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 2439 3190 4553 1239
Size: 976 224 751 1363 2579
Fragments arranged by size:
2579 1363 976 751 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 4018 4883 5181 2243
Size: 1775 865 298 2955
Fragments arranged by size:
2955 1775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2592 4556 882
Size: 617 1093 1964 2219
Fragments arranged by size:
2219 1964 1093 617
BsaAI yAC'GTr
Cuts at: 493 1204 3684 493
Size: 711 2480 2702
Fragments arranged by size:
2702 2480 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3700 4082 4717 275
Size: 53 83 186 936 2167 382 635 1451
Fragments arranged by size:
2167 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4326 4724 4998 5145 5556 4326
Size: 398 274 147 411 4663
Fragments arranged by size:
4663 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2766 2796 3163 3193 1178
Size: 30 165 30 1363 30 367 30 3878
Fragments arranged by size:
3878 1363 367 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 1186
Size: 1588 4305
BseYI C'CCAG_C
Cuts at: 2008 5096 5592 2008
Size: 3088 496 2309
Fragments arranged by size:
3088 2309 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3954 4039 5110 3954
Size: 85 1071 4737
Fragments arranged by size:
4737 1071 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3212 3954 4039 5110 1391
Size: 399 1422 742 85 1071 2174
Fragments arranged by size:
2174 1422 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 2332 3782 5512 2332
Size: 1450 1730 2713
Fragments arranged by size:
2713 1730 1450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2949 4388 4562 65
Size: 1183 1701 1439 174 1396
Fragments arranged by size:
1701 1439 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 2317 96
Size: 2221 3672
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2729 3069 794
Size: 1935 340 3618
Fragments arranged by size:
3618 1935 340
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 2135 2135
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 2531 3223 3590 3975 3992 5444 5530 5542
Size: 559 692 367 385 17 1452 86 12
Cuts at: 5542 5888 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 692 559 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2128 513
Size: 552 1063 4278
Fragments arranged by size:
4278 1063 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 2268 2727 3184 3409 4203 4223 2268
Size: 459 457 225 794 20 3938
Fragments arranged by size:
3938 794 459 457 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858
Size: 54 1425 50 275 18 1510 889 1629
Cuts at: 5858 8
Size: 43
Fragments arranged by size:
1629 1510 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2650 3823 1353
Size: 702 595 1173 3423
Fragments arranged by size:
3423 1173 702 595
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2439 3190 3956 4553 5340 5608
Size: 393 583 224 751 766 597 787 268
Cuts at: 5608 1239
Size: 1524
Fragments arranged by size:
1524 787 766 751 597 583 393 268
224
EcoNI CCTnn'n_nnAGG
Cuts at: 2166 2166
Size: 5893
EcoO109I rG'GnC_Cy
Cuts at: 2044 2498 2044
Size: 454 5439
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2528 2528
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 2766 2798 4534
Size: 32 135 32 938 32 224 32 1736
Cuts at: 4534 4566 4608 4640 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1736 938 224 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2073 3016 3237 3483 669
Size: 988 281 135 943 221 246 3079
Fragments arranged by size:
3079 988 943 281 246 221 135
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5007 5191 1305
Size: 3702 184 2007
Fragments arranged by size:
3702 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2133 2289 2342 2665 2846 3851 4210
Size: 648 967 156 53 323 181 1005 359
Cuts at: 4210 4369 518
Size: 159 2042
Fragments arranged by size:
2042 1005 967 648 359 323 181 159
156 53
MspA1I CmG'CkG
Cuts at: 1606 3309 3986 5134 5379 1606
Size: 1703 677 1148 245 2120
Fragments arranged by size:
2120 1703 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 2448 2938 387
Size: 2061 490 3342
Fragments arranged by size:
3342 2061 490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2182 2256 2347 3251 4796 1132
Size: 97 953 74 91 904 1545 2229
Fragments arranged by size:
2229 1545 953 904 97 91 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 2178 2252 4792 2178
Size: 74 2540 3279
Fragments arranged by size:
3279 2540 74
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PfoI T'CCnGG_A
Cuts at: 3042 3042
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2498 2044
Size: 454 5439
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3006 3463 3006
Size: 457 5436
PstI C_TGCA'G
Cuts at: 830 2410 2915 3245 830
Size: 1580 505 330 3478
Fragments arranged by size:
3478 1580 505 330
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SapI GCTCTTCn'nnn_
Cuts at: 2650 2650
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 2406 2549 2911 3241 4376 5057 5248
Size: 219 1361 143 362 330 1135 681 191
Cuts at: 5248 826
Size: 1471
Fragments arranged by size:
1471 1361 1135 681 362 330 219 191
143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2993 3997 4898 5160 820
Size: 197 926 279 771 1004 901 262 1553
Fragments arranged by size:
1553 1004 926 901 771 279 262 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 2571 3817 5
Size: 47 2519 1246 2081
Fragments arranged by size:
2519 2081 1246 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 3332 513
Size: 552 2267 3074
Fragments arranged by size:
3074 2267 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3920 3937 4090 4275 4720 325
Size: 1255 2340 17 153 185 445 1498
Fragments arranged by size:
2340 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2209 2317 2506
Size: 274 80 33 51 1443 232 108 189
Cuts at: 2506 4139 5851 96
Size: 1633 1712 138
Fragments arranged by size:
1712 1633 1443 274 232 189 138 108
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3163 3195 5853 5885 3163
Size: 32 2658 32 3171
Fragments arranged by size:
3171 2658 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 2473
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 3108 4022 3108
Size: 914 4979
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI