Restriction map for
pFN21AB7956
(Circular) (Six-base) MAPSORT of: pFN21AB7956.seq Check: 5401 from: 1 to: 5890
pFN21AB7956 5890 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2634 2634
Size: 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2959 3233 1937
Size: 1022 274 4594
Fragments arranged by size:
4594 1022 274
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 2926 3953 5337 5605 1632
Size: 588 706 1027 1384 268 1917
Fragments arranged by size:
1917 1384 1027 706 588 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 2034 4789 5766 1203
Size: 831 2755 977 1327
Fragments arranged by size:
2755 1327 977 831
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 2143 2800 2969 3633 5205 2143
Size: 657 169 664 1572 2828
Fragments arranged by size:
2828 1572 664 657 169
ApaI G_GGCC'C
Cuts at: 2368 2368
Size: 5890
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 3199 3425 3577 5686 1493
Size: 1706 226 152 2109 1697
Fragments arranged by size:
2109 1706 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
BamHI G'GATC_C
Cuts at: 2072 3220 3587 2072
Size: 1148 367 4375
Fragments arranged by size:
4375 1148 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 2516 3211 4666 618
Size: 325 217 627 662 67 695 1455 1842
Fragments arranged by size:
1842 1455 695 662 627 325 217 67
BanII G_rGCy'C
Cuts at: 1391 2049 2368 2438 3209 1391
Size: 658 319 70 771 4072
Fragments arranged by size:
4072 771 658 319 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 4501 136
Size: 107 122 71 631 605 646 2183 1525
Fragments arranged by size:
2183 1525 646 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 2829 3307 2829
Size: 478 5412
Bme1580I G_kGCm'C
Cuts at: 1790 2368 2535 3951 5107 1790
Size: 578 167 1416 1156 2573
Fragments arranged by size:
2573 1416 1156 578 167
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2400 2522 3144
Size: 416 53 83 399 24 983 122 622
Cuts at: 3144 3621 4579 442
Size: 477 958 1753
Fragments arranged by size:
1753 983 958 622 477 416 399 122
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2091 2102 2390 2759 2822 3065 3105
Size: 824 28 11 288 369 63 243 40
Cuts at: 3105 4550 1239
Size: 1445 2579
Fragments arranged by size:
2579 1445 824 369 288 243 63 40
28 11
Bpu10I CC'TnA_GC
Cuts at: 2697 2697
Size: 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 3143 4015 4880 5178 2642
Size: 501 872 865 298 3354
Fragments arranged by size:
3354 872 865 501 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3041 4553 882
Size: 617 1542 1512 2219
Fragments arranged by size:
2219 1542 1512 617
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2116 2159 3162 4323 4721 4995 5142 5553 2116
Size: 43 1003 1161 398 274 147 411 2453
Fragments arranged by size:
2453 1161 1003 411 398 274 147 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2314 2344 2801 2831 1178
Size: 30 165 30 911 30 457 30 4237
Fragments arranged by size:
4237 911 457 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 2660 1186
Size: 1408 66 4416
Fragments arranged by size:
4416 1408 66
BseYI C'CCAG_C
Cuts at: 2086 2437 2593 2936 2969 5093 5589 2086
Size: 351 156 343 33 2124 496 2387
Fragments arranged by size:
2387 2124 496 351 343 156 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2748 1163
Size: 573 1012 4305
Fragments arranged by size:
4305 1012 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3340 4101 5129 1540
Size: 275 186 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3951 4036 5107 3951
Size: 85 1071 4734
Fragments arranged by size:
4734 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2368 2438 2535 3209 3951 4036
Size: 399 259 319 70 97 674 742 85
Cuts at: 4036 5107 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 674 399 319 259 97
85 70
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 2634 3245 844
Size: 1190 600 611 3489
Fragments arranged by size:
3489 1190 611 600
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 1394
Size: 429 1954 3507
Fragments arranged by size:
3507 1954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4534 4721 1700
Size: 110 2724 187 2869
Fragments arranged by size:
2869 2724 187 110
BsrGI T'GTAC_A
Cuts at: 96 2537 96
Size: 2441 3449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 3950 4962 3950
Size: 1012 4878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5890
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 2008 2072 3220 3587 3972 3989 5441 5527
Size: 36 64 1148 367 385 17 1452 86
Cuts at: 5527 5539 5885 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1148 385 367 346 86 64
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2170 513
Size: 552 939 166 4233
Fragments arranged by size:
4233 939 552 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 2095 2269 3116 3406 4200 4220 2095
Size: 174 847 290 794 20 3765
Fragments arranged by size:
3765 847 794 290 174 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DraIII CAC_nnn'GTG
Cuts at: 2998 2998
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3337 4226 5855
Size: 54 1425 50 275 18 1507 889 1629
Cuts at: 5855 8
Size: 43
Fragments arranged by size:
1629 1507 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3820 1353
Size: 2467 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2728 2825 4477 4851 4997 387
Size: 1064 138 1139 97 1652 374 146 1280
Fragments arranged by size:
1652 1280 1139 1064 374 146 138 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2091 2102 2220 2390 2759 2822
Size: 393 431 28 11 118 170 369 63
Cuts at: 2822 2926 3065 3105 3953 4550 5337 5605 1239
Size: 104 139 40 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 431 393 369 268
170 139 118 104 63 40 28 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2636 2660 2636
Size: 24 5866
EcoO109I rG'GnC_Cy
Cuts at: 2364 2365 2653 2364
Size: 1 288 5601
Fragments arranged by size:
5601 288 1
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
EcoRV GAT'ATC
Cuts at: 2059 2059
Size: 5890
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 5037 1678
Size: 3359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 4531 4563 4605
Size: 32 135 32 476 32 2451 32 42
Cuts at: 4605 4637 1373
Size: 32 2626
Fragments arranged by size:
2626 2451 476 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2960 3234 3480 669
Size: 988 281 1022 274 246 3079
Fragments arranged by size:
3079 1022 988 281 274 246
HindIII A'AGCT_T
Cuts at: 748 2213 748
Size: 1465 4425
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 2034 5766 2034
Size: 3732 2158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2892 5004 5188 1305
Size: 1587 2112 184 2007
Fragments arranged by size:
2112 2007 1587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4068
Fragments arranged by size:
4068 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3180 3848 4207 4366 518
Size: 648 2014 668 359 159 2042
Fragments arranged by size:
2042 2014 668 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2842 3306 3983 5131 5376 1606
Size: 1236 464 677 1148 245 2120
Fragments arranged by size:
2120 1236 1148 677 464 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4399
Fragments arranged by size:
4399 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 3179 3248 4793 1132
Size: 97 1950 69 1545 2229
Fragments arranged by size:
2229 1950 1545 97 69
PasI CC'CwG_GG
Cuts at: 1348 2316 1348
Size: 968 4922
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2800 2969 1108
Size: 1692 169 4029
Fragments arranged by size:
4029 1692 169
PfoI T'CCnGG_A
Cuts at: 2079 2793 2079
Size: 714 5176
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 2653
Size: 5890
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PspOMI G'GGCC_C
Cuts at: 2364 2364
Size: 5890
PstI C_TGCA'G
Cuts at: 830 2847 3242 830
Size: 2017 395 3478
Fragments arranged by size:
3478 2017 395
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 2842 2842
Size: 5890
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 2958 3232 1936
Size: 1022 274 4594
Fragments arranged by size:
4594 1022 274
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2129 2843 3238 4373 5054 5245 826
Size: 219 1084 714 395 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 714 681 395 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 3122 3994 4895 5157 820
Size: 197 926 714 465 872 901 262 1553
Fragments arranged by size:
1553 926 901 872 714 465 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3179 3248 1132
Size: 2047 69 3774
Fragments arranged by size:
3774 2047 69
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 2406 1517
Size: 889 5001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2637 2820 3090 3329 513
Size: 552 939 633 183 270 239 3074
Fragments arranged by size:
3074 939 633 552 270 239 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2588 3917 3934 4087 4272 4717 325
Size: 1255 1008 1329 17 153 185 445 1498
Fragments arranged by size:
1498 1329 1255 1008 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 2537 2724
Size: 274 80 33 51 1443 261 299 187
Cuts at: 2724 4136 5848 96
Size: 1412 1712 138
Fragments arranged by size:
1712 1443 1412 299 274 261 187 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2146 2178 2801 2833 5850 5882 2146
Size: 32 623 32 3017 32 2154
Fragments arranged by size:
3017 2154 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 2216 4019 2216
Size: 1803 4087
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI