Restriction map for
pFN21AB7954
(Circular) (Six-base) MAPSORT of: pFN21AB7954.seq Check: 8860 from: 1 to: 5890
pFN21AB7954 5890 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3233 1937
Size: 1296 4594
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2583 2709 2718 2973 3038 3152 3953 5337
Size: 951 126 9 255 65 114 801 1384
Cuts at: 5337 5605 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 951 801 268 255 126 114
65 9
AflII C'TTAA_G
Cuts at: 820 1017 2276 820
Size: 197 1259 4434
Fragments arranged by size:
4434 1259 197
AflIII A'CryG_T
Cuts at: 1203 2307 4789 5766 1203
Size: 1104 2482 977 1327
Fragments arranged by size:
2482 1327 1104 977
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 2243 2771 3633 5205 2243
Size: 528 862 1572 2928
Fragments arranged by size:
2928 1572 862 528
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 2408 2605 2841 2854 3199 3425 3577 5686
Size: 915 197 236 13 345 226 152 2109
Cuts at: 5686 1493
Size: 1697
Fragments arranged by size:
2109 1697 915 345 236 226 197 152
13
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2619 2652 2619
Size: 33 5857
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2780 3211 4666 618
Size: 325 217 627 993 431 1455 1842
Fragments arranged by size:
1842 1455 993 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2095 3209 1391
Size: 704 1114 4072
Fragments arranged by size:
4072 1114 704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3158 928
Size: 971 1259 3660
Fragments arranged by size:
3660 1259 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4501 136
Size: 107 122 71 631 605 2829 1525
Fragments arranged by size:
2829 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 3307 3307
Size: 5890
Bme1580I G_kGCm'C
Cuts at: 1790 3951 5107 1790
Size: 2161 1156 2573
Fragments arranged by size:
2573 2161 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4579 442
Size: 416 53 83 399 24 2204 958 1753
Fragments arranged by size:
2204 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3066 3066
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2586 2718 4550 1239
Size: 1347 132 1832 2579
Fragments arranged by size:
2579 1832 1347 132
Bpu10I CC'TnA_GC
Cuts at: 2692 2692
Size: 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4015 4880 5178 4015
Size: 865 298 4727
Fragments arranged by size:
4727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4553 882
Size: 617 3054 2219
Fragments arranged by size:
3054 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2559 2589 3090 3120 1178
Size: 30 165 30 1156 30 501 30 3948
Fragments arranged by size:
3948 1156 501 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5890
BseYI C'CCAG_C
Cuts at: 2113 5093 5589 2113
Size: 2980 496 2414
Fragments arranged by size:
2980 2414 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3340 4101 5129 1540
Size: 275 186 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2095 3951 4036 5107 2095
Size: 1856 85 1071 2878
Fragments arranged by size:
2878 1856 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 3209 3951 4036 5107 1391
Size: 399 305 1114 742 85 1071 2174
Fragments arranged by size:
2174 1114 1071 742 399 305 85
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2042 3245 844
Size: 1198 1203 3489
Fragments arranged by size:
3489 1203 1198
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 1394
Size: 429 1954 3507
Fragments arranged by size:
3507 1954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2946 4385 4559 65
Size: 1183 1698 1439 174 1396
Fragments arranged by size:
1698 1439 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4534 4721 1700
Size: 110 2724 187 2869
Fragments arranged by size:
2869 2724 187 110
BsrGI T'GTAC_A
Cuts at: 96 2392 96
Size: 2296 3594
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 2046 3950 4962 2046
Size: 1904 1012 2974
Fragments arranged by size:
2974 1904 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5890
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885
Size: 1248 367 385 17 1452 86 12 346
Cuts at: 5885 1972
Size: 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2742 3337 4226
Size: 54 1425 50 275 18 912 595 889
Cuts at: 4226 5855 8
Size: 1629 43
Fragments arranged by size:
1629 1425 912 889 595 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2665 3033 3057 3820 1353
Size: 1312 368 24 763 3423
Fragments arranged by size:
3423 1312 763 368 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2093 2093
Size: 5890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2583 2586 2709 2718 2973 3038 3152
Size: 393 951 3 123 9 255 65 114
Cuts at: 3152 3953 4550 5337 5605 1239
Size: 801 597 787 268 1524
Fragments arranged by size:
1524 951 801 787 597 393 268 255
123 114 65 9 3
EcoRI G'AATT_C
Cuts at: 2841 3199 3577 2841
Size: 358 378 5154
Fragments arranged by size:
5154 378 358
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 5037 1678
Size: 3359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2308 2340 2982 3014 3090
Size: 32 135 32 736 32 642 32 76
Cuts at: 3090 3122 4531 4563 4605 4637 1373
Size: 32 1409 32 42 32 2626
Fragments arranged by size:
2626 1409 736 642 135 76 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2030 748
Size: 1282 4608
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2894 3129 5004 5188 1305
Size: 1589 235 1875 184 2007
Fragments arranged by size:
2007 1875 1589 235 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2744 10
Size: 54 1425 343 912 3156
Fragments arranged by size:
3156 1425 912 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 3848 4207 4366 518
Size: 648 1731 951 359 159 2042
Fragments arranged by size:
2042 1731 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3064 3306 3983 5131 5376 1606
Size: 1458 242 677 1148 245 2120
Fragments arranged by size:
2120 1458 1148 677 245 242
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5338
NdeI CA'TA_TG
Cuts at: 387 2232 387
Size: 1845 4045
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 2311 2537 3248 4793 1132
Size: 97 1082 226 711 1545 2229
Fragments arranged by size:
2229 1545 1082 711 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 2307 4789 2307
Size: 2482 3408
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2876 1108
Size: 1768 4122
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 2966 3460 2966
Size: 494 5396
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2628 2660 2628
Size: 32 5858
PstI C_TGCA'G
Cuts at: 830 2767 3031 3242 830
Size: 1937 264 211 3478
Fragments arranged by size:
3478 1937 264 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 3064 3064
Size: 5890
SacI G_AGCT'C
Cuts at: 2095 2095
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SapI GCTCTTCn'nnn_
Cuts at: 3057 3057
Size: 5890
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2103 2763 3027 3136 3238 4373 5054
Size: 219 1058 660 264 109 102 1135 681
Cuts at: 5054 5245 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1058 681 660 264 219 191
109 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 3994 4895 5157 820
Size: 197 926 333 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 333 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 3134 1517
Size: 1617 4273
StyI C'CwwG_G
Cuts at: 513 1065 2613 3329 513
Size: 552 1548 716 3074
Fragments arranged by size:
3074 1548 716 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2232 3917 3934 4087 4272 4717 325
Size: 1255 652 1685 17 153 185 445 1498
Fragments arranged by size:
1685 1498 1255 652 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2392 4136 5848
Size: 274 80 33 51 1443 415 1744 1712
Cuts at: 5848 96
Size: 138
Fragments arranged by size:
1744 1712 1443 415 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 5850 5882 2559
Size: 32 3259 32 2567
Fragments arranged by size:
3259 2567 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 2209
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 2659 3051 4019 2659
Size: 392 968 4530
Fragments arranged by size:
4530 968 392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI