Restriction map for
pFN21AB7953
(Circular) (Six-base) MAPSORT of: pFN21AB7953.seq Check: 243 from: 1 to: 5473
pFN21AB7953 5473 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3283 278
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3536 4920 5188 1632
Size: 1904 1384 268 1917
Fragments arranged by size:
1917 1904 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 4372 5349 1203
Size: 3169 977 1327
Fragments arranged by size:
3169 1327 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5473
AlwNI CAG_nnn'CTG
Cuts at: 3216 4788 3216
Size: 1572 3901
ApaLI G'TGCA_C
Cuts at: 3530 4686 3530
Size: 1156 4317
ApoI r'AATT_y
Cuts at: 1493 2782 3008 3160 5269 1493
Size: 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2798 1870
Size: 73 117 738 4545
Fragments arranged by size:
4545 738 117 73
BamHI G'GATC_C
Cuts at: 2154 2803 3170 2154
Size: 649 367 4457
Fragments arranged by size:
4457 649 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 4249 618
Size: 325 217 627 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2183 2747 2792 1391
Size: 792 564 45 4072
Fragments arranged by size:
4072 792 564 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2343 928
Size: 971 444 4058
Fragments arranged by size:
4058 971 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3664 3698 1903
Size: 34 50 34 1643 34 3678
Fragments arranged by size:
3678 1643 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2236 4084 136
Size: 107 122 71 631 605 564 1848 1525
Fragments arranged by size:
1848 1525 631 605 564 122 107 71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2114 2890 2114
Size: 776 4697
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2489 3534 4690 1790
Size: 277 422 1045 1156 2573
Fragments arranged by size:
2573 1156 1045 422 277
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3204 4162 442
Size: 416 53 83 399 24 1787 958 1753
Fragments arranged by size:
1787 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2732 4133 1239
Size: 986 507 1401 2579
Fragments arranged by size:
2579 1401 986 507
Bpu10I CC'TnA_GC
Cuts at: 2016 2016
Size: 5473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2489 3598 4463 4761 2489
Size: 1109 865 298 3201
Fragments arranged by size:
3201 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4136 882
Size: 617 2637 2219
Fragments arranged by size:
2637 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2647 3264 493
Size: 711 1443 617 2702
Fragments arranged by size:
2702 1443 711 617
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2033 3280 3662 4297
Size: 53 83 186 936 500 1247 382 635
Cuts at: 4297 275
Size: 1451
Fragments arranged by size:
1451 1247 936 635 500 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2673 3906 4304 4578 4725 5136 2673
Size: 1233 398 274 147 411 3010
Fragments arranged by size:
3010 1233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 1178
Size: 30 165 30 867 30 4351
Fragments arranged by size:
4351 867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2573 1186
Size: 1387 4086
BseYI C'CCAG_C
Cuts at: 4676 5172 4676
Size: 496 4977
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4900
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 3684 4712 1540
Size: 275 186 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 2747 3534 3619 4690 2646
Size: 101 787 85 1071 3429
Fragments arranged by size:
3429 1071 787 101 85
BsmI GAATG_Cn'
Cuts at: 2984 3077 2984
Size: 93 5380
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2183 2489 2646 2747 2792 3534
Size: 399 277 116 306 157 101 45 742
Cuts at: 3534 3619 4690 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 277 157 116
101 85 45
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 2574 2739 3362 5092 2574
Size: 165 623 1730 2955
Fragments arranged by size:
2955 1730 623 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 844
Size: 1984 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3360 1394
Size: 429 1537 3507
Fragments arranged by size:
3507 1537 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3968 4142 65
Size: 1183 2720 174 1396
Fragments arranged by size:
2720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 2635 4117 4304 1700
Size: 110 201 624 1482 187 2869
Fragments arranged by size:
2869 1482 624 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 2495 3533 4545 2495
Size: 1038 1012 3423
Fragments arranged by size:
3423 1038 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5473
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2326
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2154 2803 3170 3555 3572 5024 5110 5122
Size: 182 649 367 385 17 1452 86 12
Cuts at: 5122 5468 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 649 385 367 346 182 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2373 2373
Size: 5473
BtgI C'CryG_G
Cuts at: 513 1065 2214 2319 513
Size: 552 1149 105 3667
Fragments arranged by size:
3667 1149 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4734
Fragments arranged by size:
4734 683 56
BtsI GCAGTG_nn'
Cuts at: 2989 3783 3803 2989
Size: 794 20 4659
Fragments arranged by size:
4659 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2032 4480 809
Size: 507 716 2448 1802
Fragments arranged by size:
2448 1802 716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2568 2920 3809
Size: 54 1425 50 275 18 738 352 889
Cuts at: 3809 5438 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 738 352 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2920 1537
Size: 275 1108 4090
Fragments arranged by size:
4090 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 2697 3403 1353
Size: 1058 286 706 3423
Fragments arranged by size:
3423 1058 706 286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2654 4060 4434 4580 387
Size: 1064 138 1065 1406 374 146 1280
Fragments arranged by size:
1406 1280 1065 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2745 2745
Size: 5473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2732 3536 4133 4920 5188 1239
Size: 393 593 507 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 593 507 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2624 2624
Size: 5473
EcoO109I rG'GnC_Cy
Cuts at: 2056 2056
Size: 5473
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 5473
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 4620 1678
Size: 2942 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 4114 4146 4188
Size: 32 135 32 698 32 1812 32 42
Cuts at: 4188 4220 1373
Size: 32 2626
Fragments arranged by size:
2626 1812 698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2817 3063 669
Size: 988 281 879 246 3079
Fragments arranged by size:
3079 988 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 4587 4771 1305
Size: 711 2571 184 2007
Fragments arranged by size:
2571 2007 711 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 10
Size: 54 1425 343 738 2913
Fragments arranged by size:
2913 1425 738 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3431 3790 3949 518
Size: 648 2265 359 159 2042
Fragments arranged by size:
2265 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2755 2889 3566 4714 4959 1606
Size: 642 507 134 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 507 245 134
NaeI GCC'GGC
Cuts at: 2013 2637 2013
Size: 624 4849
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4921
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NgoMIV G'CCGG_C
Cuts at: 2011 2635 2011
Size: 624 4849
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2831 4376 1132
Size: 97 1602 1545 2229
Fragments arranged by size:
2229 1602 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5473
PfoI T'CCnGG_A
Cuts at: 2709 2709
Size: 5473
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PmlI CAC'GTG
Cuts at: 2647 2647
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 5055 5087 3542
Size: 32 1481 32 3928
Fragments arranged by size:
3928 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2056
Size: 5473
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2253 2286 2825 830
Size: 1423 33 539 3478
Fragments arranged by size:
3478 1423 539 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
PvuII CAG'CTG
Cuts at: 2248 2755 2248
Size: 507 4966
SacI G_AGCT'C
Cuts at: 2747 2747
Size: 5473
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SbfI CC_TGCA'GG
Cuts at: 2825 2825
Size: 5473
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SfcI C'TryA_G
Cuts at: 826 1045 2249 2282 2293 2821 3956 4637 4828
Size: 219 1204 33 11 528 1135 681 191
Cuts at: 4828 826
Size: 1471
Fragments arranged by size:
1471 1204 1135 681 528 219 191 33
11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 2635 1700
Size: 935 4538
SmaI CCC'GGG
Cuts at: 1872 2800 1872
Size: 928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 3577 4478 4740 820
Size: 197 926 561 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 561 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 1517
Size: 1200 4273
StyI C'CwwG_G
Cuts at: 513 1065 2912 513
Size: 552 1847 3074
Fragments arranged by size:
3074 1847 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3500 3517 3670 3855 4300 325
Size: 1255 1920 17 153 185 445 1498
Fragments arranged by size:
1920 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3719 5431 96
Size: 274 80 33 51 1443 1742 1712 138
Fragments arranged by size:
1742 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5433 5465 5433
Size: 32 5441
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5473
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 2326
Size: 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2798 1870
Size: 928 4545
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3281 276
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI