Restriction map for
pFN21AB7950
(Circular) (Six-base) MAPSORT of: pFN21AB7950.seq Check: 1087 from: 1 to: 5887
pFN21AB7950 5887 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2763 3950 5334 5602 1632
Size: 1131 1187 1384 268 1917
Fragments arranged by size:
1917 1384 1187 1131 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2751 1184
Size: 1567 4320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 2339
Size: 32 5855
AlwNI CAG_nnn'CTG
Cuts at: 2635 3630 5202 2635
Size: 995 1572 3320
Fragments arranged by size:
3320 1572 995
ApaLI G'TGCA_C
Cuts at: 2296 3944 5100 2296
Size: 1648 1156 3083
Fragments arranged by size:
3083 1648 1156
ApoI r'AATT_y
Cuts at: 1493 2189 3196 3422 3574 5683 1493
Size: 696 1007 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 696 226 152
AscI GG'CGCG_CC
Cuts at: 2442 2442
Size: 5887
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2261 2319 2397 2448 3212 1870
Size: 73 92 226 58 78 51 764 4545
Fragments arranged by size:
4545 764 226 92 78 73 58 51
BamHI G'GATC_C
Cuts at: 2683 3217 3584 2683
Size: 534 367 4986
Fragments arranged by size:
4986 534 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2164 2200 2515 3016 3208
Size: 325 217 627 377 36 315 501 192
Cuts at: 3208 4663 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 501 377 325 315 217
192 36
BanII G_rGCy'C
Cuts at: 1391 2579 3206 1391
Size: 1188 627 4072
Fragments arranged by size:
4072 1188 627
BbeI G_GCGC'C
Cuts at: 2168 2519 2168
Size: 351 5536
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4916
BbvCI CC'TCA_GC
Cuts at: 3005 3005
Size: 5887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2300 3021 3948 5104 1790
Size: 510 721 927 1156 2573
Fragments arranged by size:
2573 1156 927 721 510
BmgBI CAC'GTC
Cuts at: 1318 2217 1318
Size: 899 4988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2884 3618 4576
Size: 416 53 83 399 24 1467 734 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1467 958 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2649 2649
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2098 2429 2736 2828 2981 4547 1239
Size: 806 53 331 307 92 153 1566 2579
Fragments arranged by size:
2579 1566 806 331 307 153 92 53
Bpu10I CC'TnA_GC
Cuts at: 3005 3062 3005
Size: 57 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4877 5175 4012
Size: 865 298 4724
Fragments arranged by size:
4724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4550 882
Size: 617 3051 2219
Fragments arranged by size:
3051 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3678 493
Size: 711 2474 2702
Fragments arranged by size:
2702 2474 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2165 2468 2516 3694
Size: 53 83 186 936 632 303 48 1178
Cuts at: 3694 4076 4711 275
Size: 382 635 1451
Fragments arranged by size:
1451 1178 936 635 632 382 303 186
83 53 48
BsaWI w'CCGG_w
Cuts at: 2300 4320 4718 4992 5139 5550 2300
Size: 2020 398 274 147 411 2637
Fragments arranged by size:
2637 2020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 1178
Size: 30 165 30 936 30 4696
Fragments arranged by size:
4696 936 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2855 1186
Size: 1669 4218
BseYI C'CCAG_C
Cuts at: 2168 2337 2492 2536 5090 5586 2168
Size: 169 155 44 2554 496 2469
Fragments arranged by size:
2554 2469 496 169 155 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2470 1163
Size: 573 734 4580
Fragments arranged by size:
4580 734 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2428 3337 4098 5126 1540
Size: 275 186 427 909 761 1028 2301
Fragments arranged by size:
2301 1028 909 761 427 275 186
BsiHKAI G_wGCw'C
Cuts at: 2300 3948 4033 5104 2300
Size: 1648 85 1071 3083
Fragments arranged by size:
3083 1648 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
BsmBI CGTCTCn'nnnn_
Cuts at: 2298 2298
Size: 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2579 3021 3206 3948 4033 5104
Size: 399 510 279 442 185 742 85 1071
Cuts at: 5104 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 510 442 399 279 185
85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2991 3242 844
Size: 2147 251 3489
Fragments arranged by size:
3489 2147 251
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2090 2157 2385 3774 1394
Size: 429 236 31 67 228 1389 3507
Fragments arranged by size:
3507 1389 429 236 228 67 31
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2051 2427 2504 2650 3141 4531
Size: 110 230 11 376 77 146 491 1390
Cuts at: 4531 4718 1700
Size: 187 2869
Fragments arranged by size:
2869 1390 491 376 230 187 146 110
77 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 2442 1925
Size: 517 5370
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3154 794
Size: 2360 3527
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 2653 1184
Size: 1469 4418
BstYI r'GATC_y
Cuts at: 1972 2683 3217 3584 3969 3986 5438 5524 5536
Size: 711 534 367 385 17 1452 86 12
Cuts at: 5536 5882 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 711 534 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2160 2282 513
Size: 552 1095 122 4118
Fragments arranged by size:
4118 1095 552 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2190 487
Size: 683 56 964 4184
Fragments arranged by size:
4184 964 683 56
BtsI GCAGTG_nn'
Cuts at: 3403 4197 4217 3403
Size: 794 20 5073
Fragments arranged by size:
5073 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3027 3334 4223
Size: 54 1425 50 275 18 1197 307 889
Cuts at: 4223 5852 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1197 889 307 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2618 2766 2793 2844 3101 3817 1353
Size: 1265 148 27 51 257 716 3423
Fragments arranged by size:
3423 1265 716 257 148 51 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2461 2989 4474 4848 4994 387
Size: 1064 138 872 528 1485 374 146 1280
Fragments arranged by size:
1485 1280 1064 872 528 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2098 2429 2736 2763 2828 2981
Size: 393 413 53 331 307 27 65 153
Cuts at: 2981 3950 4547 5334 5602 1239
Size: 969 597 787 268 1524
Fragments arranged by size:
1524 969 787 597 413 393 331 307
268 153 65 53 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3065 3065
Size: 5887
EcoO109I rG'GnC_Cy
Cuts at: 2237 2251 2789 3043 2237
Size: 14 538 254 5081
Fragments arranged by size:
5081 538 254 14
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2168 2519 3005 5034 1678
Size: 490 351 486 2029 2531
Fragments arranged by size:
2531 2029 490 486 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2845 2877 2932
Size: 32 135 32 518 32 723 32 55
Cuts at: 2932 2964 4528 4560 4602 4634 1373
Size: 32 1564 32 42 32 2626
Fragments arranged by size:
2626 1564 723 518 135 55 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KasI G'GCGC_C
Cuts at: 2164 2515 2164
Size: 351 5536
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3029 10
Size: 54 1425 343 1197 2868
Fragments arranged by size:
2868 1425 1197 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2663 2733 3845 4204 4363 518
Size: 648 843 654 70 1112 359 159 2042
Fragments arranged by size:
2042 1112 843 654 648 359 159 70
MspA1I CmG'CkG
Cuts at: 1606 2536 3033 3303 3980 5128 5373 1606
Size: 930 497 270 677 1148 245 2120
Fragments arranged by size:
2120 1148 930 677 497 270 245
NaeI GCC'GGC
Cuts at: 2042 2506 3143 2042
Size: 464 637 4786
Fragments arranged by size:
4786 637 464
NarI GG'CG_CC
Cuts at: 2165 2516 2165
Size: 351 5536
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5335
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NgoMIV G'CCGG_C
Cuts at: 2040 2504 3141 2040
Size: 464 637 4786
Fragments arranged by size:
4786 637 464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NruI TCG'CGA
Cuts at: 2992 2992
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2380 3245 4790 1132
Size: 97 1151 865 1545 2229
Fragments arranged by size:
2229 1545 1151 865 97
PasI CC'CwG_GG
Cuts at: 1348 2226 1348
Size: 878 5009
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PfoI T'CCnGG_A
Cuts at: 2958 2958
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2339 2371 3956 3988 5469 5501 2339
Size: 32 1585 32 1481 32 2725
Fragments arranged by size:
2725 1585 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2237 2251 2789 2237
Size: 14 538 5335
Fragments arranged by size:
5335 538 14
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 2824 3239 830
Size: 1994 415 3478
Fragments arranged by size:
3478 1994 415
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 3033 3033
Size: 5887
RsrII CG'GwC_CG
Cuts at: 2054 2054
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SanDI GG'GwC_CC
Cuts at: 2237 2251 2789 2237
Size: 14 538 5335
Fragments arranged by size:
5335 538 14
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SexAI A'CCwGG_T
Cuts at: 3012 3012
Size: 5887
SfcI C'TryA_G
Cuts at: 826 1045 2820 2871 3117 3235 4370 5051 5242
Size: 219 1775 51 246 118 1135 681 191
Cuts at: 5242 826
Size: 1471
Fragments arranged by size:
1775 1471 1135 681 246 219 191 118
51
SfoI GGC'GCC
Cuts at: 2166 2517 2166
Size: 351 5536
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 2263 2321 2399 2450 3214 1872
Size: 391 58 78 51 764 4545
Fragments arranged by size:
4545 764 391 78 58 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3991 4892 5154 820
Size: 197 926 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 2558 3071 1517
Size: 1041 513 4333
Fragments arranged by size:
4333 1041 513
StyI C'CwwG_G
Cuts at: 513 1065 2463 3039 3072 3326 513
Size: 552 1398 576 33 254 3074
Fragments arranged by size:
3074 1398 576 552 254 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3914 3931 4084 4269 4714 325
Size: 1255 2334 17 153 185 445 1498
Fragments arranged by size:
2334 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 5845 96
Size: 274 80 33 51 1443 2156 1712 138
Fragments arranged by size:
2156 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 2261 2319 2397 2448 3212 1870
Size: 391 58 78 51 764 4545
Fragments arranged by size:
4545 764 391 78 58 51
XmnI GAAnn'nnTTC
Cuts at: 2189 2699 2802 4016 2189
Size: 510 103 1214 4060
Fragments arranged by size:
4060 1214 510 103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NsiI PacI PmlI PspOMI PspXI PsrI SacI SacII
SapI SfiI SrfI SwaI XcmI