Restriction map for
pFN21AB7941
(Circular) (Six-base) MAPSORT of: pFN21AB7941.seq Check: 9411 from: 1 to: 5734
pFN21AB7941 5734 bp
With 182 enzymes: SgfI *
March 28, 2012 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2665 2910 2665
Size: 245 5489
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 2040 3055 2040
Size: 1015 4719
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 3797 5181 5449 1632
Size: 716 1449 1384 268 1917
Fragments arranged by size:
1917 1449 1384 716 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 2880 4633 5610 1203
Size: 1677 1753 977 1327
Fragments arranged by size:
1753 1677 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2585 2617 2585
Size: 32 5702
AlwNI CAG_nnn'CTG
Cuts at: 2767 2902 3477 5049 2767
Size: 135 575 1572 3452
Fragments arranged by size:
3452 1572 575 135
ApaI G_GGCC'C
Cuts at: 2215 2364 2822 2849 2215
Size: 149 458 27 5100
Fragments arranged by size:
5100 458 149 27
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2257 2356 2498 2848 2950 2960 3059
Size: 73 314 99 142 350 102 10 99
Cuts at: 3059 1870
Size: 4545
Fragments arranged by size:
4545 350 314 142 102 99 99 73
10
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2488 3055 4510 618
Size: 325 217 627 253 448 567 1455 1842
Fragments arranged by size:
1842 1455 627 567 448 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2215 2255 2364 2822 2849 3053 1391
Size: 824 40 109 458 27 204 4072
Fragments arranged by size:
4072 824 458 204 109 40 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2557 928
Size: 971 658 4105
Fragments arranged by size:
4105 971 658
BbvCI CC'TCA_GC
Cuts at: 2798 2798
Size: 5734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2203 2219 2277
Size: 107 122 71 631 605 531 16 58
Cuts at: 2277 2817 4345 136
Size: 540 1528 1525
Fragments arranged by size:
1528 1525 631 605 540 531 122 107
71 58 16
BglII A'GATC_T
Cuts at: 2456 5729 2456
Size: 3273 2461
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2181 2215 2364 2493 2822 2849 3795 4951
Size: 391 34 149 129 329 27 946 1156
Cuts at: 4951 1790
Size: 2573
Fragments arranged by size:
2573 1156 946 391 329 149 129 34
27
BmgBI CAC'GTC
Cuts at: 1318 2575 2806 1318
Size: 1257 231 4246
Fragments arranged by size:
4246 1257 231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 3465 4423
Size: 416 53 83 399 24 660 1388 958
Cuts at: 4423 442
Size: 1753
Fragments arranged by size:
1753 1388 958 660 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2418 2418
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2375 2464 2612 4394 1239
Size: 859 277 89 148 1782 2579
Fragments arranged by size:
2579 1782 859 277 148 89
Bpu10I CC'TnA_GC
Cuts at: 2798 2798
Size: 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4397 882
Size: 617 2898 2219
Fragments arranged by size:
2898 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 2141 5728 2141
Size: 3587 2147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2585 2615 2870
Size: 30 165 30 834 30 318 30 255
Cuts at: 2870 2900 1178
Size: 30 4012
Fragments arranged by size:
4012 834 318 255 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2344 2849 2852 2855 3005 3014 1186
Size: 826 332 505 3 3 150 9 3906
Fragments arranged by size:
3906 826 505 332 150 9 3 3
BseYI C'CCAG_C
Cuts at: 2380 2470 2656 2858 2895 4937 5433 2380
Size: 90 186 202 37 2042 496 2681
Fragments arranged by size:
2681 2042 496 202 186 90 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2597 1163
Size: 573 861 4300
Fragments arranged by size:
4300 861 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2635 3184 3945 4973 1540
Size: 275 186 634 549 761 1028 2301
Fragments arranged by size:
2301 1028 761 634 549 275 186
BsiHKAI G_wGCw'C
Cuts at: 3795 3880 4951 3795
Size: 85 1071 4578
Fragments arranged by size:
4578 1071 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
BsmBI CGTCTCn'nnnn_
Cuts at: 2309 2309
Size: 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2215 2255 2364 2493 2822 2849
Size: 399 391 34 40 109 129 329 27
Cuts at: 2849 3053 3795 3880 4951 1391
Size: 204 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 329 204 129
109 85 40 34 27
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2665 2910 3089 844
Size: 1328 493 245 179 3489
Fragments arranged by size:
3489 1328 493 245 179
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 2525 1925
Size: 600 5134
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2767 794
Size: 1973 3761
BstBI TT'CG_AA
Cuts at: 2430 4538 2430
Size: 2108 3626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2131 2456 3064 3431 3816 3833 5285 5371
Size: 159 325 608 367 385 17 1452 86
Cuts at: 5371 5383 5729 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 608 385 367 346 325 159
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2217 2912 513
Size: 552 1152 695 3335
Fragments arranged by size:
3335 1152 695 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 3250 4044 4064 3250
Size: 794 20 4920
Fragments arranged by size:
4920 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2513 2649
Size: 54 1425 50 275 18 277 406 136
Cuts at: 2649 3181 4070 5699 8
Size: 532 889 1629 43
Fragments arranged by size:
1629 1425 889 532 406 277 275 136
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 2991 3664 1353
Size: 1473 165 673 3423
Fragments arranged by size:
3423 1473 673 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2277 2728 4321 4695 4841 387
Size: 1064 138 688 451 1593 374 146 1280
Fragments arranged by size:
1593 1280 1064 688 451 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2348 2375 2464 2612 3797 4394
Size: 393 466 250 27 89 148 1185 597
Cuts at: 4394 5181 5449 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1185 787 597 466 393 268 250
148 89 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 2126
Size: 5734
EcoO109I rG'GnC_Cy
Cuts at: 2211 2212 2238 2261 2360 2502 2818 2819 2845
Size: 1 26 23 99 142 316 1 26
Cuts at: 2845 2908 2211
Size: 63 5037
Fragments arranged by size:
5037 316 142 99 63 26 26 23
1 1
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 2591 4881 1678
Size: 913 2290 2531
Fragments arranged by size:
2531 2290 913
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 4375 4407 4449
Size: 32 135 32 665 32 2106 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 2106 665 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 2044 3059 2044
Size: 1015 4719
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 2880 5610 2880
Size: 2730 3004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2595 2913 4848 5032 1305
Size: 1290 318 1935 184 2007
Fragments arranged by size:
2007 1935 1290 318 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3912
Fragments arranged by size:
3912 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3692 4051 4210 518
Size: 648 2526 359 159 2042
Fragments arranged by size:
2526 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2518 2581 2914 2929 3150 3827 4975 5220
Size: 912 63 333 15 221 677 1148 245
Cuts at: 5220 1606
Size: 2120
Fragments arranged by size:
2120 1148 912 677 333 245 221 63
15
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5182
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NruI TCG'CGA
Cuts at: 2757 2757
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2194 2551 3092 4637 1132
Size: 97 965 357 541 1545 2229
Fragments arranged by size:
2229 1545 965 541 357 97
PasI CC'CwG_GG
Cuts at: 1348 2822 1348
Size: 1474 4260
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2869 1108
Size: 1761 3973
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2585 2617 3803 3835 5316 5348 2585
Size: 32 1186 32 1481 32 2971
Fragments arranged by size:
2971 1481 1186 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2261 2502 2908 2261
Size: 241 406 5087
Fragments arranged by size:
5087 406 241
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspOMI G'GGCC_C
Cuts at: 2211 2360 2818 2845 2211
Size: 149 458 27 5100
Fragments arranged by size:
5100 458 149 27
PspXI vC'TCGA_Gb
Cuts at: 2950 2950
Size: 5734
PstI C_TGCA'G
Cuts at: 830 3086 830
Size: 2256 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
SacII CC_GC'GG
Cuts at: 2915 2915
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SanDI GG'GwC_CC
Cuts at: 2261 2908 2261
Size: 647 5087
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 3082 4217 4898 5089 826
Size: 219 2037 1135 681 191 1471
Fragments arranged by size:
2037 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2219 2817 2219
Size: 598 5136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 2259 2500 2850 2962 3061 1872
Size: 387 241 350 112 99 4545
Fragments arranged by size:
4545 387 350 241 112 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2950 3838 4739 5001 820
Size: 197 926 1007 888 901 262 1553
Fragments arranged by size:
1553 1007 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 2551 3092 1132
Size: 1419 541 3774
Fragments arranged by size:
3774 1419 541
SrfI GCCC'GGGC
Cuts at: 2850 2850
Size: 5734
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2721 3173 513
Size: 552 1656 452 3074
Fragments arranged by size:
3074 1656 552 452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3761 3778 3931 4116 4561 325
Size: 1255 2181 17 153 185 445 1498
Fragments arranged by size:
2181 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 3980 5692
Size: 274 80 33 51 1443 639 1364 1712
Cuts at: 5692 96
Size: 138
Fragments arranged by size:
1712 1443 1364 639 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2870 2902 5694 5726 2870
Size: 32 2792 32 2878
Fragments arranged by size:
2878 2792 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2122 2819 2866 2122
Size: 697 47 4990
Fragments arranged by size:
4990 697 47
XhoI C'TCGA_G
Cuts at: 1943 2950 1943
Size: 1007 4727
XmaI C'CCGG_G
Cuts at: 1870 2257 2498 2848 2960 3059 1870
Size: 387 241 350 112 99 4545
Fragments arranged by size:
4545 387 350 241 112 99
XmnI GAAnn'nnTTC
Cuts at: 2600 3863 2600
Size: 1263 4471
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PfoI PmlI PsrI
PvuII RsrII SacI SapI SexAI SfoI SwaI