Restriction map for
pFN21AB7939
(Circular) (Six-base) MAPSORT of: pFN21AB7939.seq Check: 574 from: 1 to: 5731
pFN21AB7939 5731 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2800 2800
Size: 5731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 2138 2274 3794 5178 5446 1632
Size: 441 65 136 1520 1384 268 1917
Fragments arranged by size:
1917 1520 1384 441 268 136 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 2096 2739 3474 5046 2096
Size: 643 735 1572 2781
Fragments arranged by size:
2781 1572 735 643
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 2147 2667 3040 3266 3418 5527 1493
Size: 654 520 373 226 152 2109 1697
Fragments arranged by size:
2109 1697 654 520 373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2277 3056 1870
Size: 73 334 779 4545
Fragments arranged by size:
4545 779 334 73
BamHI G'GATC_C
Cuts at: 2128 2717 3061 3428 2128
Size: 589 344 367 4431
Fragments arranged by size:
4431 589 367 344
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2691 3052 4507 618
Size: 325 217 627 904 361 1455 1842
Fragments arranged by size:
1842 1455 904 627 361 325 217
BanII G_rGCy'C
Cuts at: 1391 2537 3050 1391
Size: 1146 513 4072
Fragments arranged by size:
4072 1146 513
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2079 2262 2505 928
Size: 971 116 64 183 243 4154
Fragments arranged by size:
4154 971 243 183 116 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2292 2753 5726 2292
Size: 461 2973 2297
Fragments arranged by size:
2973 2297 461
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 3792 4948 1790
Size: 2002 1156 2573
Fragments arranged by size:
2573 2002 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3004 4391 1239
Size: 1765 1387 2579
Fragments arranged by size:
2579 1765 1387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2631 3856 4721 5019 2631
Size: 1225 865 298 3343
Fragments arranged by size:
3343 1225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2734 2795 2986 1186
Size: 1548 61 191 3931
Fragments arranged by size:
3931 1548 191 61
BseYI C'CCAG_C
Cuts at: 4934 5430 4934
Size: 496 5235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3792 3877 4948 3792
Size: 85 1071 4575
Fragments arranged by size:
4575 1071 85
BsmI GAATG_Cn'
Cuts at: 2036 3242 3335 2036
Size: 1206 93 4432
Fragments arranged by size:
4432 1206 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 3050 3792 3877 4948 1391
Size: 399 747 513 742 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 513 399 85
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2800 3086 844
Size: 1956 286 3489
Fragments arranged by size:
3489 1956 286
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 2961 3791 4803 2961
Size: 830 1012 3889
Fragments arranged by size:
3889 1012 830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 2068 1184
Size: 884 4847
BstYI r'GATC_y
Cuts at: 1972 2128 2292 2717 2753 3061 3428 3813 3830
Size: 156 164 425 36 308 367 385 17
Cuts at: 3830 5282 5368 5380 5726 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 425 385 367 346 308 164
156 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2862 2862
Size: 5731
BtgI C'CryG_G
Cuts at: 513 1065 2239 2722 513
Size: 552 1174 483 3522
Fragments arranged by size:
3522 1174 552 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2420 487
Size: 683 56 1194 3798
Fragments arranged by size:
3798 1194 683 56
BtsI GCAGTG_nn'
Cuts at: 3247 4041 4061 3247
Size: 794 20 4917
Fragments arranged by size:
4917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2924 3178 4067
Size: 54 1425 50 275 18 1094 254 889
Cuts at: 4067 5696 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1094 889 275 254 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 3661 1353
Size: 1536 772 3423
Fragments arranged by size:
3423 1536 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2138 2274 3004 3794 4391 5178
Size: 393 441 65 136 730 790 597 787
Cuts at: 5178 5446 1239
Size: 268 1524
Fragments arranged by size:
1524 790 787 730 597 441 393 268
136 65
EcoO109I rG'GnC_Cy
Cuts at: 2093 2093
Size: 5731
EcoRI G'AATT_C
Cuts at: 2667 3040 3418 2667
Size: 373 378 4980
Fragments arranged by size:
4980 378 373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2623 2314
Size: 309 5422
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 4878 1678
Size: 3200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2310 2342 2402 2434 2729
Size: 32 135 32 738 32 60 32 295
Cuts at: 2729 2761 4372 4404 4446 4478 1373
Size: 32 1611 32 42 32 2626
Fragments arranged by size:
2626 1611 738 295 135 60 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 2479 4845 5029 1305
Size: 945 229 2366 184 2007
Fragments arranged by size:
2366 2007 945 229 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2926 10
Size: 54 1425 343 1094 2815
Fragments arranged by size:
2815 1425 1094 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 3689 4048 4207 518
Size: 648 1297 1226 359 159 2042
Fragments arranged by size:
2042 1297 1226 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3015 3147 3824 4972 5217 1606
Size: 1409 132 677 1148 245 2120
Fragments arranged by size:
2120 1409 1148 677 245 132
NcoI C'CATG_G
Cuts at: 513 1065 2239 513
Size: 552 1174 4005
Fragments arranged by size:
4005 1174 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2468 2715 3089 4634 1132
Size: 97 1239 247 374 1545 2229
Fragments arranged by size:
2229 1545 1239 374 247 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PfoI T'CCnGG_A
Cuts at: 2981 2981
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2800 2846 2879 3083 830
Size: 1970 46 33 204 3478
Fragments arranged by size:
3478 1970 204 46 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 3015 3015
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2796 2842 2875 3079 4214 4895 5086
Size: 219 1751 46 33 204 1135 681 191
Cuts at: 5086 826
Size: 1471
Fragments arranged by size:
1751 1471 1135 681 219 204 191 46
33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2279 3058 1872
Size: 407 779 4545
Fragments arranged by size:
4545 779 407
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2646 3835 4736 4998 820
Size: 197 926 703 1189 901 262 1553
Fragments arranged by size:
1553 1189 926 901 703 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SrfI GCCC'GGGC
Cuts at: 2279 2279
Size: 5731
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2239 2889 2991 3027 3170 513
Size: 552 1174 650 102 36 143 3074
Fragments arranged by size:
3074 1174 650 552 143 102 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3758 3775 3928 4113 4558 325
Size: 1255 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2412 3977 5689
Size: 274 80 33 51 1443 435 1565 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1712 1565 1443 435 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 5691 5723 2436
Size: 32 3223 32 2444
Fragments arranged by size:
3223 2444 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 2685 2369
Size: 316 5415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2277 3056 1870
Size: 407 779 4545
Fragments arranged by size:
4545 779 407
XmnI GAAnn'nnTTC
Cuts at: 2151 3860 2151
Size: 1709 4022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SwaI