Restriction map for
pFN21AB7937
(Circular) (Six-base) MAPSORT of: pFN21AB7937.seq Check: 4480 from: 1 to: 5833
pFN21AB7937 5833 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2435 2605 2917 2435
Size: 170 312 5351
Fragments arranged by size:
5351 312 170
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 2868 2937 3896 5280 5548 1632
Size: 845 391 69 959 1384 268 1917
Fragments arranged by size:
1917 1384 959 845 391 268 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2409 1184
Size: 1225 4608
AlwNI CAG_nnn'CTG
Cuts at: 2620 3576 5148 2620
Size: 956 1572 3305
Fragments arranged by size:
3305 1572 956
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2476 3053 3142 3368 3520 5629 1493
Size: 983 577 89 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 577 226 152 89
AseI AT'TA_AT
Cuts at: 160 1032 2070 4387 160
Size: 872 1038 2317 1606
Fragments arranged by size:
2317 1606 1038 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2516 3154 4609 618
Size: 325 217 627 729 638 1455 1842
Fragments arranged by size:
1842 1455 729 638 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2943 3152 1391
Size: 1552 209 4072
Fragments arranged by size:
4072 1552 209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2877 2901 928
Size: 971 978 24 3860
Fragments arranged by size:
3860 978 971 24
BbvCI CC'TCA_GC
Cuts at: 2280 2295 2538 2673 2280
Size: 15 243 135 5440
Fragments arranged by size:
5440 243 135 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2830 3725 4935 2830
Size: 895 1210 3728
Fragments arranged by size:
3728 1210 895
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BfrBI ATG'CAT
Cuts at: 2396 2396
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2048 5828 2048
Size: 3780 2053
BlpI GC'TnA_GC
Cuts at: 2274 3250 2274
Size: 976 4857
Bme1580I G_kGCm'C
Cuts at: 1790 2521 3894 5050 1790
Size: 731 1373 1156 2573
Fragments arranged by size:
2573 1373 1156 731
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2841 3564 4522
Size: 416 53 83 399 24 1424 723 958
Cuts at: 4522 442
Size: 1753
Fragments arranged by size:
1753 1424 958 723 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2655 4493 1239
Size: 1416 1838 2579
Fragments arranged by size:
2579 1838 1416
Bpu10I CC'TnA_GC
Cuts at: 2280 2295 2431 2538 2673 2280
Size: 15 136 107 135 5440
Fragments arranged by size:
5440 136 135 107 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2688 3047 3958 4823 5121 2688
Size: 359 911 865 298 3400
Fragments arranged by size:
3400 911 865 359 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2788 2818 1178
Size: 30 165 30 1385 30 4193
Fragments arranged by size:
4193 1385 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2616 2903 1186
Size: 1430 287 4116
Fragments arranged by size:
4116 1430 287
BseYI C'CCAG_C
Cuts at: 2232 2310 2784 5036 5532 2232
Size: 78 474 2252 496 2533
Fragments arranged by size:
2533 2252 496 474 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3894 3979 5050 3894
Size: 85 1071 4677
Fragments arranged by size:
4677 1071 85
BsmI GAATG_Cn'
Cuts at: 2398 3344 3437 2398
Size: 946 93 4794
Fragments arranged by size:
4794 946 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2521 2943 3152 3894 3979 5050 1391
Size: 399 731 422 209 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 731 422 399 209 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 2435 2605 2917 3188 844
Size: 1461 130 170 312 271 3489
Fragments arranged by size:
3489 1461 312 271 170 130
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2441 794
Size: 1647 4186
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2048 3163 3530 3915 3932 5384 5470 5482
Size: 76 1115 367 385 17 1452 86 12
Cuts at: 5482 5828 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1115 385 367 346 86 76
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4342
Fragments arranged by size:
4342 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 3280 4169
Size: 54 1425 50 275 18 934 516 889
Cuts at: 4169 5798 8
Size: 1629 43
Fragments arranged by size:
1629 1425 934 889 516 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2634 2931 3085 3763 1353
Size: 831 450 297 154 678 3423
Fragments arranged by size:
3423 831 678 450 297 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 2655 2868 2937 3896 4493 5280
Size: 393 845 178 213 69 959 597 787
Cuts at: 5280 5548 1239
Size: 268 1524
Fragments arranged by size:
1524 959 845 787 597 393 268 213
178 69
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2278 2310 2828 2860 2970
Size: 32 135 32 706 32 518 32 110
Cuts at: 2970 3002 4474 4506 4548 4580 1373
Size: 32 1472 32 42 32 2626
Fragments arranged by size:
2626 1472 706 518 135 110 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4947 5131 1305
Size: 3642 184 2007
Fragments arranged by size:
3642 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2766 10
Size: 54 1425 343 934 3077
Fragments arranged by size:
3077 1425 934 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 3791 4150 4309 518
Size: 648 1225 1400 359 159 2042
Fragments arranged by size:
2042 1400 1225 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2423 3249 3926 5074 5319 1606
Size: 817 826 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 817 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4342
Fragments arranged by size:
4342 939 552
NdeI CA'TA_TG
Cuts at: 387 2388 387
Size: 2001 3832
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NsiI A_TGCA'T
Cuts at: 2398 2398
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PfoI T'CCnGG_A
Cuts at: 2632 2632
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2657 1613
Size: 1044 4789
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2821 2853 2821
Size: 32 5801
PstI C_TGCA'G
Cuts at: 830 2446 3185 830
Size: 1616 739 3478
Fragments arranged by size:
3478 1616 739
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2423 2423
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SapI GCTCTTCn'nnn_
Cuts at: 2184 2931 2184
Size: 747 5086
SbfI CC_TGCA'GG
Cuts at: 2446 3185 2446
Size: 739 5094
ScaI AGT'ACT
Cuts at: 2118 4081 5793 2118
Size: 1963 1712 2158
Fragments arranged by size:
2158 1963 1712
SfcI C'TryA_G
Cuts at: 826 1045 2418 2442 3181 4316 4997 5188 826
Size: 219 1373 24 739 1135 681 191 1471
Fragments arranged by size:
1471 1373 1135 739 681 219 191 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2703 3062 3937 4838 5100 820
Size: 197 926 760 359 875 901 262 1553
Fragments arranged by size:
1553 926 901 875 760 359 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 3272 513
Size: 552 939 1268 3074
Fragments arranged by size:
3074 1268 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3860 3877 4030 4215 4660 325
Size: 1255 2280 17 153 185 445 1498
Fragments arranged by size:
2280 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2116 2593 4079
Size: 274 80 33 51 1443 139 477 1486
Cuts at: 4079 5791 96
Size: 1712 138
Fragments arranged by size:
1712 1486 1443 477 274 139 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI