Restriction map for
pFN21AB7933
(Circular) (Six-base) MAPSORT of: pFN21AB7933.seq Check: 2683 from: 1 to: 5806
pFN21AB7933 5806 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 2571 3127 2571
Size: 556 5250
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2518 3869 5253 5521 1632
Size: 608 278 1351 1384 268 1917
Fragments arranged by size:
1917 1384 1351 608 278 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 3081 4705 5682 1203
Size: 1878 1624 977 1327
Fragments arranged by size:
1878 1624 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 2835 4535 2835
Size: 1700 4106
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 3549 5121 3549
Size: 1572 4234
ApaI G_GGCC'C
Cuts at: 2796 2796
Size: 5806
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2335 2522 2795 3131 1870
Size: 73 392 187 273 336 4545
Fragments arranged by size:
4545 392 336 273 187 73
BamHI G'GATC_C
Cuts at: 2681 2995 3136 3503 2681
Size: 314 141 367 4984
Fragments arranged by size:
4984 367 314 141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2198 2571 3127 4582
Size: 325 217 627 243 168 373 556 1455
Cuts at: 4582 618
Size: 1842
Fragments arranged by size:
1842 1455 627 556 373 325 243 217
168
BanII G_rGCy'C
Cuts at: 1391 2244 2507 2529 2639 2796 2806 3101 3125
Size: 853 263 22 110 157 10 295 24
Cuts at: 3125 1391
Size: 4072
Fragments arranged by size:
4072 853 295 263 157 110 24 22
10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2105 2768 928
Size: 971 206 663 3966
Fragments arranged by size:
3966 971 663 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2133 3698 4908 2133
Size: 1565 1210 3031
Fragments arranged by size:
3031 1565 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2339 2426 2524
Size: 107 122 71 631 605 667 87 98
Cuts at: 2524 2900 4417 136
Size: 376 1517 1525
Fragments arranged by size:
1525 1517 667 631 605 376 122 107
98 87 71
BglII A'GATC_T
Cuts at: 2745 5801 2745
Size: 3056 2750
BlpI GC'TnA_GC
Cuts at: 2054 2589 3223 2054
Size: 535 634 4637
Fragments arranged by size:
4637 634 535
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2201 2796 3867 5023 1790
Size: 243 168 595 1071 1156 2573
Fragments arranged by size:
2573 1156 1071 595 243 168
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2665 2777 4466 1239
Size: 1426 112 1689 2579
Fragments arranged by size:
2579 1689 1426 112
Bpu10I CC'TnA_GC
Cuts at: 2309 2501 2309
Size: 192 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3931 4796 5094 2927
Size: 1004 865 298 3639
Fragments arranged by size:
3639 1004 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2716 4469 882
Size: 617 1217 1753 2219
Fragments arranged by size:
2219 1753 1217 617
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2606 3613 3995 4630
Size: 53 83 186 936 1073 1007 382 635
Cuts at: 4630 275
Size: 1451
Fragments arranged by size:
1451 1073 1007 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2366 2735 4239 4637 4911 5058 5469 2366
Size: 369 1504 398 274 147 411 2703
Fragments arranged by size:
2703 1504 411 398 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2140 2143 2164 2365 2368 2371 2395 1186
Size: 954 3 21 201 3 3 24 4597
Fragments arranged by size:
4597 954 201 24 21 3 3 3
BseYI C'CCAG_C
Cuts at: 2540 5009 5505 2540
Size: 2469 496 2841
Fragments arranged by size:
2841 2469 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 3062 1163
Size: 573 327 999 3907
Fragments arranged by size:
3907 999 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2280 2442 2553 3256 4017 5045
Size: 275 186 279 162 111 703 761 1028
Cuts at: 5045 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 703 279 275 186 162
111
BsiHKAI G_wGCw'C
Cuts at: 3101 3867 3952 5023 3101
Size: 766 85 1071 3884
Fragments arranged by size:
3884 1071 766 85
BsmI GAATG_Cn'
Cuts at: 2045 3317 3410 2045
Size: 1272 93 4441
Fragments arranged by size:
4441 1272 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2201 2244 2507 2529 2639 2796
Size: 399 243 168 43 263 22 110 157
Cuts at: 2796 2806 3101 3125 3867 3952 5023 1391
Size: 10 295 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 295 263 243 168
157 110 85 43 24 22 10
BspEI T'CCGG_A
Cuts at: 2735 5469 2735
Size: 2734 3072
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2028 4301 4475 65
Size: 1183 780 2273 174 1396
Fragments arranged by size:
2273 1396 1183 780 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 2313 2568 4450 4637 1700
Size: 110 328 175 255 1882 187 2869
Fragments arranged by size:
2869 1882 328 255 187 175 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 2719 3866 4878 2719
Size: 1147 1012 3647
Fragments arranged by size:
3647 1147 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5806
BstBI TT'CG_AA
Cuts at: 2769 4610 2769
Size: 1841 3965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstXI CCAn_nnnn'nTGG
Cuts at: 2329 2584 2692 2329
Size: 255 108 5443
Fragments arranged by size:
5443 255 108
BstYI r'GATC_y
Cuts at: 1972 2681 2745 2995 3136 3503 3888 3905 5357
Size: 709 64 250 141 367 385 17 1452
Cuts at: 5357 5443 5455 5801 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 709 385 367 346 250 141
86 64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2232 2328 2424 2436 2547 2553 2562
Size: 552 1167 96 96 12 111 6 9
Cuts at: 2562 2904 513
Size: 342 3415
Fragments arranged by size:
3415 1167 552 342 111 96 96 12
9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 3322 4116 4136 3322
Size: 794 20 4992
Fragments arranged by size:
4992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2088 2229
Size: 54 1425 50 275 18 240 18 141
Cuts at: 2229 2277 2439 2475 2550 2556 2599 2901 2907
Size: 48 162 36 75 6 43 302 6
Cuts at: 2907 3253 4142 5771 8
Size: 346 889 1629 43
Fragments arranged by size:
1629 1425 889 346 302 275 240 162
141 75 54 50 48 43 43 36
18 18 6 6
EagI C'GGCC_G
Cuts at: 1537 1812 2277 2439 2550 3253 1537
Size: 275 465 162 111 703 4090
Fragments arranged by size:
4090 703 465 275 162 111
EarI CTCTTCn'nnn_
Cuts at: 1353 2389 2702 2961 3736 1353
Size: 1036 313 259 775 3423
Fragments arranged by size:
3423 1036 775 313 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2344 2383 2398 4393 4767 4913
Size: 1064 138 755 39 15 1995 374 146
Cuts at: 4913 387
Size: 1280
Fragments arranged by size:
1995 1280 1064 755 374 146 138 39
15
EcoICRI GAG'CTC
Cuts at: 3099 3099
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2518 2665 2777 3869 4466 5253
Size: 393 608 278 147 112 1092 597 787
Cuts at: 5253 5521 1239
Size: 268 1524
Fragments arranged by size:
1524 1092 787 608 597 393 278 268
147 112
EcoNI CCTnn'n_nnAGG
Cuts at: 2676 2676
Size: 5806
EcoO109I rG'GnC_Cy
Cuts at: 2792 2949 2792
Size: 157 5649
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
EcoRV GAT'ATC
Cuts at: 2305 2305
Size: 5806
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2547 2941 4953 1678
Size: 869 394 2012 2531
Fragments arranged by size:
2531 2012 869 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2730 2762 2821 2853 2987
Size: 32 135 32 1158 32 59 32 134
Cuts at: 2987 3019 3086 3118 4447 4479 4521 4553 1373
Size: 32 67 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 1158 135 134 67 59 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 2575 3131 2575
Size: 556 5250
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 3081 5682 3081
Size: 2601 3205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2673 4920 5104 1305
Size: 1368 2247 184 2007
Fragments arranged by size:
2247 2007 1368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2903 10
Size: 54 1425 343 1071 2913
Fragments arranged by size:
2913 1425 1071 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 3764 4123 4282 518
Size: 648 1161 1437 359 159 2042
Fragments arranged by size:
2042 1437 1161 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2131 2426 2438 2549 2584 3028 3054
Size: 501 24 295 12 111 35 444 26
Cuts at: 3054 3222 3899 5047 5292 1606
Size: 168 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 444 295 245 168
111 35 26 24 12
NaeI GCC'GGC
Cuts at: 2140 2315 2140
Size: 175 5631
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NgoMIV G'CCGG_C
Cuts at: 2138 2313 2138
Size: 175 5631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 2439 3253 2439
Size: 814 4992
NspI r_CATG'y
Cuts at: 1132 1229 3164 4709 1132
Size: 97 1935 1545 2229
Fragments arranged by size:
2229 1935 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2616 1108
Size: 1508 4298
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PspOMI G'GGCC_C
Cuts at: 2792 2792
Size: 5806
PstI C_TGCA'G
Cuts at: 830 3158 830
Size: 2328 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 3028 3028
Size: 5806
SacI G_AGCT'C
Cuts at: 3101 3101
Size: 5806
SacII CC_GC'GG
Cuts at: 2427 2439 2550 2427
Size: 12 111 5683
Fragments arranged by size:
5683 111 12
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 3154 4289 4970 5161 826
Size: 219 2109 1135 681 191 1471
Fragments arranged by size:
2109 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2339 2339
Size: 5806
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2524 3133 1872
Size: 652 609 4545
Fragments arranged by size:
4545 652 609
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2942 3910 4811 5073 820
Size: 197 926 999 968 901 262 1553
Fragments arranged by size:
1553 999 968 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SrfI GCCC'GGGC
Cuts at: 2524 2524
Size: 5806
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 3245 513
Size: 552 2180 3074
Fragments arranged by size:
3074 2180 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2216 3833 3850 4003 4188 4633 325
Size: 1255 636 1617 17 153 185 445 1498
Fragments arranged by size:
1617 1498 1255 636 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5766 5798 5766
Size: 32 5774
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2522 3131 1870
Size: 652 609 4545
Fragments arranged by size:
4545 652 609
XmnI GAAnn'nnTTC
Cuts at: 2765 3935 2765
Size: 1170 4636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI
SfoI SwaI