Restriction map for
pFN21AB7930
(Circular) (Six-base) MAPSORT of: pFN21AB7930.seq Check: 1528 from: 1 to: 5344
pFN21AB7930 5344 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 1937
Size: 750 4594
Acc65I G'GTAC_C
Cuts at: 2665 2665
Size: 5344
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3407 4791 5059 1632
Size: 725 1050 1384 268 1917
Fragments arranged by size:
1917 1384 1050 725 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5147
AflIII A'CryG_T
Cuts at: 1203 4243 5220 1203
Size: 3040 977 1327
Fragments arranged by size:
3040 1327 977
AgeI A'CCGG_T
Cuts at: 4175 4175
Size: 5344
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5344
AlwNI CAG_nnn'CTG
Cuts at: 3087 4659 3087
Size: 1572 3772
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2653 2879 3031 5140 1493
Size: 1160 226 152 2109 1697
Fragments arranged by size:
2109 1697 1160 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 1870
Size: 73 726 4545
Fragments arranged by size:
4545 726 73
BamHI G'GATC_C
Cuts at: 2674 3041 2674
Size: 367 4977
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2665 4120 618
Size: 325 217 627 878 1455 1842
Fragments arranged by size:
1842 1455 878 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2663 1391
Size: 1272 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3535 3569 1903
Size: 34 1598 34 3678
Fragments arranged by size:
3678 1598 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2419 3236 4446 2419
Size: 817 1210 3317
Fragments arranged by size:
3317 1210 817
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3955 136
Size: 107 122 71 631 605 2283 1525
Fragments arranged by size:
2283 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2278 5339 2278
Size: 3061 2283
BlpI GC'TnA_GC
Cuts at: 2761 2761
Size: 5344
Bme1580I G_kGCm'C
Cuts at: 1790 2238 3405 4561 1790
Size: 448 1167 1156 2573
Fragments arranged by size:
2573 1167 1156 448
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2088 3075 4033
Size: 416 53 83 399 24 671 987 958
Cuts at: 4033 442
Size: 1753
Fragments arranged by size:
1753 987 958 671 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 2306 4004 1239
Size: 927 140 1698 2579
Fragments arranged by size:
2579 1698 927 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 3469 4334 4632 2477
Size: 992 865 298 3189
Fragments arranged by size:
3189 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4007 882
Size: 617 2508 2219
Fragments arranged by size:
2508 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3135 493
Size: 711 1931 2702
Fragments arranged by size:
2702 1931 711
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3151 3533 4168 275
Size: 53 83 186 936 1618 382 635 1451
Fragments arranged by size:
1618 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3777 4175 4449 4596 5007 3777
Size: 398 274 147 411 4114
Fragments arranged by size:
4114 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5119
Fragments arranged by size:
5119 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5344
BseYI C'CCAG_C
Cuts at: 4547 5043 4547
Size: 496 4848
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 1163
Size: 573 294 4477
Fragments arranged by size:
4477 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2794 3555 4583 1540
Size: 275 186 793 761 1028 2301
Fragments arranged by size:
2301 1028 793 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 3405 3490 4561 2175
Size: 1230 85 1071 2958
Fragments arranged by size:
2958 1230 1071 85
BsmI GAATG_Cn'
Cuts at: 2233 2855 2948 2233
Size: 622 93 4629
Fragments arranged by size:
4629 622 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2238 2663 3405 3490 4561 1391
Size: 399 385 63 425 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 425 399 385 85 63
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 3233 4963 3233
Size: 1730 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 844
Size: 1855 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3231 1394
Size: 429 1408 3507
Fragments arranged by size:
3507 1408 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3839 4013 65
Size: 1183 2591 174 1396
Fragments arranged by size:
2591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3988 4175 1700
Size: 110 2178 187 2869
Fragments arranged by size:
2869 2178 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5344
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5344
BssSI C'ACGA_G
Cuts at: 3404 4416 3404
Size: 1012 4332
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5344
BstBI TT'CG_AA
Cuts at: 4148 4148
Size: 5344
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5344
BstYI r'GATC_y
Cuts at: 1972 2278 2674 3041 3426 3443 4895 4981 4993
Size: 306 396 367 385 17 1452 86 12
Cuts at: 4993 5339 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 396 385 367 346 306 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3853
Fragments arranged by size:
3853 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2524 487
Size: 683 56 1298 3307
Fragments arranged by size:
3307 1298 683 56
BtsI GCAGTG_nn'
Cuts at: 2860 3654 3674 2860
Size: 794 20 4530
Fragments arranged by size:
4530 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5309
DraI TTT'AAA
Cuts at: 2140 2648 2140
Size: 508 4836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2791 3680 5309
Size: 54 1425 50 275 18 961 889 1629
Cuts at: 5309 8
Size: 43
Fragments arranged by size:
1629 1425 961 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2791 1537
Size: 275 979 4090
Fragments arranged by size:
4090 979 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 3274 1353
Size: 1023 898 3423
Fragments arranged by size:
3423 1023 898
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2011 3931 4305 4451 387
Size: 1064 138 422 1920 374 146 1280
Fragments arranged by size:
1920 1280 1064 422 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2306 2357 3407 4004 4791 5059
Size: 393 534 140 51 1050 597 787 268
Cuts at: 5059 1239
Size: 1524
Fragments arranged by size:
1524 1050 787 597 534 393 268 140
51
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2297 2297
Size: 5344
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 4491 1678
Size: 2813 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2567 2599 3985 4017 4059
Size: 32 135 32 995 32 1386 32 42
Cuts at: 4059 4091 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 995 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 2934 669
Size: 988 281 750 246 3079
Fragments arranged by size:
3079 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KpnI G_GTAC'C
Cuts at: 2669 2669
Size: 5344
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4458 4642 1305
Size: 3153 184 2007
Fragments arranged by size:
3153 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3522
Fragments arranged by size:
3522 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3302 3661 3820 518
Size: 648 2136 359 159 2042
Fragments arranged by size:
2136 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2440 2760 3437 4585 4830 1606
Size: 514 320 320 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 514 320 320 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3853
Fragments arranged by size:
3853 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2702 4247 1132
Size: 97 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5344
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5344
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4926 4958 3413
Size: 32 1481 32 3799
Fragments arranged by size:
3799 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PstI C_TGCA'G
Cuts at: 830 2696 830
Size: 1866 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
PvuII CAG'CTG
Cuts at: 2120 2120
Size: 5344
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2692 3827 4508 4699 826
Size: 219 1647 1135 681 191 1471
Fragments arranged by size:
1647 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5344
SmaI CCC'GGG
Cuts at: 1872 2671 1872
Size: 799 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 3448 4349 4611 820
Size: 197 926 549 956 901 262 1553
Fragments arranged by size:
1553 956 926 901 549 262 197
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SspI AAT'ATT
Cuts at: 5 52 2244 3268 5
Size: 47 2192 1024 2081
Fragments arranged by size:
2192 2081 1024 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5344
StyI C'CwwG_G
Cuts at: 513 1065 2004 2783 513
Size: 552 939 779 3074
Fragments arranged by size:
3074 939 779 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3371 3388 3541 3726 4171 325
Size: 1255 1791 17 153 185 445 1498
Fragments arranged by size:
1791 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2252 3590 5302
Size: 274 80 33 51 1443 275 1338 1712
Cuts at: 5302 96
Size: 138
Fragments arranged by size:
1712 1443 1338 275 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5304 5336 5304
Size: 32 5312
Tth111I GACn'n_nGTC
Cuts at: 1316 2094 1316
Size: 778 4566
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2669 1870
Size: 799 4545
XmnI GAAnn'nnTTC
Cuts at: 2280 2301 3473 2280
Size: 21 1172 4151
Fragments arranged by size:
4151 1172 21
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI