Restriction map for
pFN21AB7926
(Circular) (Six-base) MAPSORT of: pFN21AB7926.seq Check: 3967 from: 1 to: 5755
pFN21AB7926 5755 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 3065 3818 5202 5470 1632
Size: 574 859 753 1384 268 1917
Fragments arranged by size:
1917 1384 859 753 574 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2863 4654 5631 1203
Size: 1660 1791 977 1327
Fragments arranged by size:
1791 1660 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2873 3064 3290 3442 5551 1493
Size: 1380 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 1380 226 191 152
AseI AT'TA_AT
Cuts at: 160 1032 2373 4309 160
Size: 872 1341 1936 1606
Fragments arranged by size:
1936 1606 1341 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 3076 4531 618
Size: 325 217 627 353 936 1455 1842
Fragments arranged by size:
1842 1455 936 627 353 325 217
BanII G_rGCy'C
Cuts at: 1391 2164 3074 1391
Size: 773 910 4072
Fragments arranged by size:
4072 910 773
BbeI G_GCGC'C
Cuts at: 2144 2144
Size: 5755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2447 928
Size: 971 548 4236
Fragments arranged by size:
4236 971 548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2045 2079 3946 3980 1903
Size: 34 108 34 1867 34 3678
Fragments arranged by size:
3678 1867 108 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 2601 2932 1548
Size: 1053 331 4371
Fragments arranged by size:
4371 1053 331
BfrBI ATG'CAT
Cuts at: 2635 2635
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2711 5750 2711
Size: 3039 2716
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 3816 4972 1790
Size: 2026 1156 2573
Fragments arranged by size:
2573 2026 1156
BmgBI CAC'GTC
Cuts at: 1318 2975 1318
Size: 1657 4098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2294 2348 2795 4415 1239
Size: 977 78 54 447 1620 2579
Fragments arranged by size:
2579 1620 977 447 78 54
Bpu10I CC'TnA_GC
Cuts at: 2190 2190
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 3562 3944 4579
Size: 53 83 186 936 608 1421 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1451 1421 936 635 608 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2146 2176 1178
Size: 30 165 30 743 30 4757
Fragments arranged by size:
4757 743 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2264 2282 2291 2303 2306 2309 2312
Size: 958 120 18 9 12 3 3 3
Cuts at: 2312 2372 2375 3030 1186
Size: 60 3 655 3911
Fragments arranged by size:
3911 958 655 120 60 18 12 9
3 3 3 3
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2097 1163
Size: 573 361 4821
Fragments arranged by size:
4821 573 361
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
BsmBI CGTCTCn'nnnn_
Cuts at: 2147 2147
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2164 3074 3816 3901 4972 1391
Size: 399 374 910 742 85 1071 2174
Fragments arranged by size:
2174 1071 910 742 399 374 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2683 3110 844
Size: 1839 427 3489
Fragments arranged by size:
3489 1839 427
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3000 4250 4424 65
Size: 1183 1752 1250 174 1396
Fragments arranged by size:
1752 1396 1250 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2137 2337 4399 4586 1700
Size: 110 327 200 2062 187 2869
Fragments arranged by size:
2869 2062 327 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 2589 96
Size: 2493 3262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2711 3085 3452 3837 3854 5306 5392 5404
Size: 739 374 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 739 385 374 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720
Size: 54 1425 50 275 18 1372 889 1629
Cuts at: 5720 8
Size: 43
Fragments arranged by size:
1629 1425 1372 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2280 3685 1353
Size: 906 21 1405 3423
Fragments arranged by size:
3423 1405 906 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2070 4342 4716 4862 387
Size: 1064 138 481 2272 374 146 1280
Fragments arranged by size:
2272 1280 1064 481 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2216 2294 2348 2795 3065 3818
Size: 393 574 10 78 54 447 270 753
Cuts at: 3818 4415 5202 5470 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 574 447 393 270
268 78 54 10
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2046 2144 2368 4902 1678
Size: 368 98 224 2534 2531
Fragments arranged by size:
2534 2531 368 224 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 2530 2562 4396
Size: 32 135 32 574 32 352 32 1834
Cuts at: 4396 4428 4470 4502 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1834 574 352 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2140 2140
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 2544 2922 2995 4869 5053 1305
Size: 930 309 378 73 1874 184 2007
Fragments arranged by size:
2007 1874 930 378 309 184 73
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4072 4231 518
Size: 648 2547 359 159 2042
Fragments arranged by size:
2547 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2123 3171 3848 4996 5241 1606
Size: 517 1048 677 1148 245 2120
Fragments arranged by size:
2120 1148 1048 677 517 245
NaeI GCC'GGC
Cuts at: 2339 2339
Size: 5755
NarI GG'CG_CC
Cuts at: 2141 2141
Size: 5755
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2337 2337
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NsiI A_TGCA'T
Cuts at: 2637 2637
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2867 3113 4658 1132
Size: 97 1638 246 1545 2229
Fragments arranged by size:
2229 1638 1545 246 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 2863 4654 2863
Size: 1791 3964
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2307 1613
Size: 694 5061
PsiI TTA'TAA
Cuts at: 2553 3325 2553
Size: 772 4983
PstI C_TGCA'G
Cuts at: 830 2068 3107 830
Size: 1238 1039 3478
Fragments arranged by size:
3478 1238 1039
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2064 2497 3103 4238 4919 5110 826
Size: 219 1019 433 606 1135 681 191 1471
Fragments arranged by size:
1471 1135 1019 681 606 433 219 191
SfoI GGC'GCC
Cuts at: 2142 2142
Size: 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 3194 513
Size: 552 939 1190 3074
Fragments arranged by size:
3074 1190 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2583 3782 3799 3952 4137 4582 325
Size: 1255 1003 1199 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1199 1003 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2530 2589 4001
Size: 274 80 33 51 1443 553 59 1412
Cuts at: 4001 5713 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1412 553 274 138 80 59
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2676 2708 5715 5747 2676
Size: 32 3007 32 2684
Fragments arranged by size:
3007 2684 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 2608 2659 3091 2608
Size: 51 432 5272
Fragments arranged by size:
5272 432 51
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 2454 3884 2454
Size: 1430 4325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI