Restriction map for
pFN21AB7924
(Circular) (Six-base) MAPSORT of: pFN21AB7924.seq Check: 515 from: 1 to: 5743
pFN21AB7924 5743 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 2602 3064 2602
Size: 462 5281
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2868 3806 5190 5458 1632
Size: 818 418 938 1384 268 1917
Fragments arranged by size:
1917 1384 938 818 418 268
AflII C'TTAA_G
Cuts at: 820 1017 2947 820
Size: 197 1930 3616
Fragments arranged by size:
3616 1930 197
AflIII A'CryG_T
Cuts at: 1203 2830 2934 4642 5619 1203
Size: 1627 104 1708 977 1327
Fragments arranged by size:
1708 1627 1327 977 104
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2854 3486 5058 2854
Size: 632 1572 3539
Fragments arranged by size:
3539 1572 632
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2274 3052 3278 3430 5539 1493
Size: 781 778 226 152 2109 1697
Fragments arranged by size:
2109 1697 781 778 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2502 4297 160
Size: 872 1470 1795 1606
Fragments arranged by size:
1795 1606 1470 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2126 3068 1870
Size: 73 86 97 942 4545
Fragments arranged by size:
4545 942 97 86 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2132 2578 2602 3064
Size: 325 217 627 254 91 446 24 462
Cuts at: 3064 4519 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 462 446 325 254 217
91 24
BanII G_rGCy'C
Cuts at: 1391 2022 2030 3062 1391
Size: 631 8 1032 4072
Fragments arranged by size:
4072 1032 631 8
BbeI G_GCGC'C
Cuts at: 2045 2136 2045
Size: 91 5652
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2538 2919 928
Size: 971 639 381 3752
Fragments arranged by size:
3752 971 639 381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2348 3635 4845 2348
Size: 1287 1210 3246
Fragments arranged by size:
3246 1287 1210
BclI T'GATC_A
Cuts at: 1548 2446 1548
Size: 898 4845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2050 2576
Size: 107 122 71 631 605 337 41 526
Cuts at: 2576 4354 136
Size: 1778 1525
Fragments arranged by size:
1778 1525 631 605 526 337 122 107
71 41
BglII A'GATC_T
Cuts at: 2419 5738 2419
Size: 3319 2424
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 3804 4960 1790
Size: 2014 1156 2573
Fragments arranged by size:
2573 2014 1156
BmgBI CAC'GTC
Cuts at: 1318 2935 1318
Size: 1617 4126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2761 3474 4432
Size: 416 53 83 399 24 1344 713 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1344 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 2763 4403 1239
Size: 1379 145 1640 2579
Fragments arranged by size:
2579 1640 1379 145
Bpu10I CC'TnA_GC
Cuts at: 2823 2823
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2133 3550 3932
Size: 53 83 186 936 509 91 1417 382
Cuts at: 3932 4567 275
Size: 635 1451
Fragments arranged by size:
1451 1417 936 635 509 382 186 91
83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5518
Fragments arranged by size:
5518 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5743
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2072 3193 3954 4982 1540
Size: 275 186 71 1121 761 1028 2301
Fragments arranged by size:
2301 1121 1028 761 275 186 71
BsiHKAI G_wGCw'C
Cuts at: 2030 3804 3889 4960 2030
Size: 1774 85 1071 2813
Fragments arranged by size:
2813 1774 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2030 3062 3804 3889 4960 1391
Size: 399 232 8 1032 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 742 399 232 85 8
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 2527 3632 5362 2527
Size: 1105 1730 2908
Fragments arranged by size:
2908 1730 1105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 4387 4574 1700
Size: 110 234 2343 187 2869
Fragments arranged by size:
2869 2343 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 2156 3803 4815 2156
Size: 1647 1012 3084
Fragments arranged by size:
3084 1647 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2227 2419 2891 3073 3440 3825 3842 5294
Size: 255 192 472 182 367 385 17 1452
Cuts at: 5294 5380 5392 5738 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 472 385 367 346 255 192
182 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2718 513
Size: 552 939 714 3538
Fragments arranged by size:
3538 939 714 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 2568 3190
Size: 54 1425 50 275 18 239 499 622
Cuts at: 3190 4079 5708 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 622 499 275 239 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2069 3190 1537
Size: 275 257 1121 4090
Fragments arranged by size:
4090 1121 275 257
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 3673 1353
Size: 803 1517 3423
Fragments arranged by size:
3423 1517 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2028 2028
Size: 5743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2618 2763 2868 3806 4403 5190
Size: 393 818 168 145 105 938 597 787
Cuts at: 5190 5458 1239
Size: 268 1524
Fragments arranged by size:
1524 938 818 787 597 393 268 168
145 105
EcoO109I rG'GnC_Cy
Cuts at: 2669 2669
Size: 5743
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
EcoRV GAT'ATC
Cuts at: 2284 2399 2908 2284
Size: 115 509 5119
Fragments arranged by size:
5119 509 115
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2045 2136 4890 1678
Size: 367 91 2754 2531
Fragments arranged by size:
2754 2531 367 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2941 4384 4416 4458
Size: 32 135 32 1337 32 1443 32 42
Cuts at: 4458 4490 1373
Size: 32 2626
Fragments arranged by size:
2626 1443 1337 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2041 2132 2041
Size: 91 5652
KpnI G_GTAC'C
Cuts at: 2606 3068 2606
Size: 462 5281
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 10
Size: 54 1425 343 738 3183
Fragments arranged by size:
3183 1425 738 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3701 4060 4219 518
Size: 648 2535 359 159 2042
Fragments arranged by size:
2535 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 3159 3836 4984 5229 1606
Size: 462 1091 677 1148 245 2120
Fragments arranged by size:
2120 1148 1091 677 462 245
NaeI GCC'GGC
Cuts at: 2046 2046
Size: 5743
NarI GG'CG_CC
Cuts at: 2042 2133 2042
Size: 91 5652
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NgoMIV G'CCGG_C
Cuts at: 2044 2044
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 2069 3190 2069
Size: 1121 4622
NspI r_CATG'y
Cuts at: 1132 1229 2834 3101 4646 1132
Size: 97 1605 267 1545 2229
Fragments arranged by size:
2229 1605 1545 267 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 2830 4642 2830
Size: 1812 3931
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2669 2669
Size: 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 2690 3313 2690
Size: 623 5120
PstI C_TGCA'G
Cuts at: 830 2205 2859 3095 830
Size: 1375 654 236 3478
Fragments arranged by size:
3478 1375 654 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SacI G_AGCT'C
Cuts at: 2030 2030
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 2156 2156
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2201 2465 2855 2871 3091 4226 4907
Size: 219 1156 264 390 16 220 1135 681
Cuts at: 4907 5098 826
Size: 191 1471
Fragments arranged by size:
1471 1156 1135 681 390 264 220 219
191 16
SfoI GGC'GCC
Cuts at: 2043 2134 2043
Size: 91 5652
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2947 3847 4748 5010 820
Size: 197 926 1004 900 901 262 1553
Fragments arranged by size:
1553 1004 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 3182 513
Size: 552 939 207 971 3074
Fragments arranged by size:
3074 971 939 552 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 2422 3079 2422
Size: 657 5086
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 2614
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SrfI SwaI