Restriction map for
pFN21AB7920
(Circular) (Six-base) MAPSORT of: pFN21AB7920.seq Check: 7834 from: 1 to: 5743
pFN21AB7920 5743 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2757 2757
Size: 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2510 3086 1937
Size: 573 576 4594
Fragments arranged by size:
4594 576 573
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2836 3806 5190 5458 1632
Size: 1204 970 1384 268 1917
Fragments arranged by size:
1917 1384 1204 970 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 3486 5058 3486
Size: 1572 4171
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2146 2238 3052 3278 3430 5539 1493
Size: 653 92 814 226 152 2109 1697
Fragments arranged by size:
2109 1697 814 653 226 152 92
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2904 3068 1870
Size: 73 961 164 4545
Fragments arranged by size:
4545 961 164 73
BamHI G'GATC_C
Cuts at: 2529 3073 3440 2529
Size: 544 367 4832
Fragments arranged by size:
4832 544 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2254 3064 4519 618
Size: 325 217 627 467 810 1455 1842
Fragments arranged by size:
1842 1455 810 627 467 325 217
BanII G_rGCy'C
Cuts at: 1391 2340 2736 3062 1391
Size: 949 396 326 4072
Fragments arranged by size:
4072 949 396 326
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4772
BbvCI CC'TCA_GC
Cuts at: 2250 2754 2250
Size: 504 5239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2839 3635 4845 2839
Size: 796 1210 3737
Fragments arranged by size:
3737 1210 796
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4354 136
Size: 107 122 71 631 605 337 2345 1525
Fragments arranged by size:
2345 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 2604 3160 2604
Size: 556 5187
Bme1580I G_kGCm'C
Cuts at: 1790 2570 3804 4960 1790
Size: 780 1234 1156 2573
Fragments arranged by size:
2573 1234 1156 780
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2249 1056
Size: 1193 4550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2414 4403 1239
Size: 968 207 1989 2579
Fragments arranged by size:
2579 1989 968 207
Bpu10I CC'TnA_GC
Cuts at: 2250 2754 2250
Size: 504 5239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 3868 4733 5031 2750
Size: 1118 865 298 3462
Fragments arranged by size:
3462 1118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2494 3534 493
Size: 711 1290 1040 2702
Fragments arranged by size:
2702 1290 1040 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2105 2868 4176 4574 4848 4995 5406 2105
Size: 763 1308 398 274 147 411 2442
Fragments arranged by size:
2442 1308 763 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5518
Fragments arranged by size:
5518 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2771 1186
Size: 1585 4158
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2816 1163
Size: 573 1080 4090
Fragments arranged by size:
4090 1080 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3804 3889 4960 3804
Size: 85 1071 4587
Fragments arranged by size:
4587 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
BsmBI CGTCTCn'nnnn_
Cuts at: 2432 2432
Size: 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2570 2736 3062 3804 3889 4960
Size: 399 550 230 166 326 742 85 1071
Cuts at: 4960 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 550 399 326 230 166
85
BspEI T'CCGG_A
Cuts at: 2868 5406 2868
Size: 2538 3205
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2640 2757 3098 844
Size: 1796 117 341 3489
Fragments arranged by size:
3489 1796 341 117
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2077 2217 4238 4412 65
Size: 1183 829 140 2021 174 1396
Fragments arranged by size:
2021 1396 1183 829 174 140
BsrFI r'CCGG_y
Cuts at: 1700 1810 3003 4387 4574 1700
Size: 110 1193 1384 187 2869
Fragments arranged by size:
2869 1384 1193 187 110
BsrGI T'GTAC_A
Cuts at: 96 2286 96
Size: 2190 3553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2219 794
Size: 1425 4318
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2529 3073 3440 3825 3842 5294 5380 5392
Size: 557 544 367 385 17 1452 86 12
Cuts at: 5392 5738 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 557 544 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2511 2511
Size: 5743
BtgI C'CryG_G
Cuts at: 513 1065 2004 2736 513
Size: 552 939 732 3520
Fragments arranged by size:
3520 939 732 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2584 487
Size: 683 56 1358 3646
Fragments arranged by size:
3646 1358 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DraIII CAC_nnn'GTG
Cuts at: 2506 2783 2506
Size: 277 5466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2617 2820 3190
Size: 54 1425 50 275 18 787 203 370
Cuts at: 3190 4079 5708 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 787 370 275 203 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3673 1353
Size: 2320 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2414 2836 3806 4403 5190 5458
Size: 393 575 207 422 970 597 787 268
Cuts at: 5458 1239
Size: 1524
Fragments arranged by size:
1524 970 787 597 575 422 393 268
207
EcoO109I rG'GnC_Cy
Cuts at: 2278 2278
Size: 5743
EcoRI G'AATT_C
Cuts at: 2238 3052 3430 2238
Size: 814 378 4551
Fragments arranged by size:
4551 814 378
EcoRV GAT'ATC
Cuts at: 2593 2593
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 3010 4890 1678
Size: 1332 1880 2531
Fragments arranged by size:
2531 1880 1332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2308 2340 2398
Size: 32 135 32 593 32 111 32 58
Cuts at: 2398 2430 4384 4416 4458 4490 1373
Size: 32 1954 32 42 32 2626
Fragments arranged by size:
2626 1954 593 135 111 58 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3087 3333 669
Size: 988 281 1002 147 246 3079
Fragments arranged by size:
3079 1002 988 281 246 147
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2599 4857 5041 1305
Size: 1294 2258 184 2007
Fragments arranged by size:
2258 2007 1294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2619 2822 10
Size: 54 1425 343 787 203 2931
Fragments arranged by size:
2931 1425 787 343 203 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 3701 4060 4219 518
Size: 648 1006 1529 359 159 2042
Fragments arranged by size:
2042 1529 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2608 2753 3159 3836 4984 5229 1606
Size: 1002 145 406 677 1148 245 2120
Fragments arranged by size:
2120 1148 1002 677 406 245 145
NaeI GCC'GGC
Cuts at: 3005 3005
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 2520 387
Size: 2133 3610
NgoMIV G'CCGG_C
Cuts at: 3003 3003
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 2245 1052
Size: 1193 4550
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2536 1348
Size: 1188 4555
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2278
Size: 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PstI C_TGCA'G
Cuts at: 830 3095 830
Size: 2265 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2608 2753 2608
Size: 145 5598
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2988 3991 5703 2988
Size: 1003 1712 3028
Fragments arranged by size:
3028 1712 1003
SexAI A'CCwGG_T
Cuts at: 2779 2779
Size: 5743
SfcI C'TryA_G
Cuts at: 826 1045 2417 3091 4226 4907 5098 826
Size: 219 1372 674 1135 681 191 1471
Fragments arranged by size:
1471 1372 1135 681 674 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2906 3070 1872
Size: 1034 164 4545
Fragments arranged by size:
4545 1034 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3847 4748 5010 820
Size: 197 926 786 1118 901 262 1553
Fragments arranged by size:
1553 1118 926 901 786 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 2383 2806 3182 513
Size: 552 939 379 423 376 3074
Fragments arranged by size:
3074 939 552 423 379 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2164 2286 2986
Size: 274 80 33 51 1443 187 122 700
Cuts at: 2986 3989 5701 96
Size: 1003 1712 138
Fragments arranged by size:
1712 1443 1003 700 274 187 138 122
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2839 2839
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 2904 3068 1870
Size: 1034 164 4545
Fragments arranged by size:
4545 1034 164
XmnI GAAnn'nnTTC
Cuts at: 2147 3872 2147
Size: 1725 4018
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI Bsu36I EcoICRI EcoNI FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI