Restriction map for
pFN21AB7917
(Circular) (Six-base) MAPSORT of: pFN21AB7917.seq Check: 1171 from: 1 to: 5815
pFN21AB7917 5815 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2149 2502 3878 5262 5530 1632
Size: 517 353 1376 1384 268 1917
Fragments arranged by size:
1917 1384 1376 517 353 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 2574 4714 5691 1203
Size: 1371 2140 977 1327
Fragments arranged by size:
2140 1371 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 2059 2272 4544 2059
Size: 213 2272 3330
Fragments arranged by size:
3330 2272 213
AleI CACnn'nnGTG
Cuts at: 1166 2292 1166
Size: 1126 4689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 2038 3558 5130 2038
Size: 1520 1572 2723
Fragments arranged by size:
2723 1572 1520
ApaI G_GGCC'C
Cuts at: 2098 2098
Size: 5815
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 3010 3124 3350 3502 5611 1493
Size: 1517 114 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 226 152 114
AseI AT'TA_AT
Cuts at: 160 1032 2937 4369 160
Size: 872 1905 1432 1606
Fragments arranged by size:
1905 1606 1432 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3140 1870
Size: 73 1197 4545
Fragments arranged by size:
4545 1197 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2540 3048 3136 4591
Size: 325 217 627 225 528 508 88 1455
Cuts at: 4591 618
Size: 1842
Fragments arranged by size:
1842 1455 627 528 508 325 225 217
88
BanII G_rGCy'C
Cuts at: 1391 2098 2257 2312 3134 1391
Size: 707 159 55 822 4072
Fragments arranged by size:
4072 822 707 159 55
BbeI G_GCGC'C
Cuts at: 2016 3052 2016
Size: 1036 4779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2512 2802 928
Size: 971 613 290 3941
Fragments arranged by size:
3941 971 613 290
BbvCI CC'TCA_GC
Cuts at: 3084 3084
Size: 5815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2090 2124 3026 3060 4006 4040 1903
Size: 34 153 34 902 34 946 34 3678
Fragments arranged by size:
3678 946 902 153 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 2529 1548
Size: 981 4834
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 4426 136
Size: 107 122 71 631 605 404 2350 1525
Fragments arranged by size:
2350 1525 631 605 404 122 107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2098 3876 5032 1790
Size: 308 1778 1156 2573
Fragments arranged by size:
2573 1778 1156 308
BmgBI CAC'GTC
Cuts at: 1318 2659 1318
Size: 1341 4474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 3546 4504
Size: 416 53 83 399 24 882 1247 958
Cuts at: 4504 442
Size: 1753
Fragments arranged by size:
1753 1247 958 882 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2999 1056
Size: 1943 3872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2120 2510 4475 1239
Size: 821 60 390 1965 2579
Fragments arranged by size:
2579 1965 821 390 60
Bpu10I CC'TnA_GC
Cuts at: 3084 3084
Size: 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4805 5103 3940
Size: 865 298 4652
Fragments arranged by size:
4652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2399 2666 4478 882
Size: 617 900 267 1812 2219
Fragments arranged by size:
2219 1812 900 617 267
BsaAI yAC'GTr
Cuts at: 493 1204 2577 2636 3606 493
Size: 711 1373 59 970 2702
Fragments arranged by size:
2702 1373 970 711 59
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 2060 3049 3622
Size: 53 83 186 936 480 47 989 573
Cuts at: 3622 4004 4639 275
Size: 382 635 1451
Fragments arranged by size:
1451 989 936 635 573 480 382 186
83 53 47
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2688 2718 1178
Size: 30 165 30 1285 30 4275
Fragments arranged by size:
4275 1285 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5815
BseYI C'CCAG_C
Cuts at: 2073 2211 2595 2775 5018 5514 2073
Size: 138 384 180 2243 496 2374
Fragments arranged by size:
2374 2243 496 384 180 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2150 2364 3876 3961 5032 2150
Size: 214 1512 85 1071 2933
Fragments arranged by size:
2933 1512 1071 214 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
BsmBI CGTCTCn'nnnn_
Cuts at: 2665 2665
Size: 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2150 2257 2312 2364 3134 3876
Size: 399 308 52 107 55 52 770 742
Cuts at: 3876 3961 5032 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 770 742 399 308 107 85
55 52 52
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3170 844
Size: 2326 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2259 3118 4310 4484 65
Size: 1183 1011 859 1192 174 1396
Fragments arranged by size:
1396 1192 1183 1011 859 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 2079 1925
Size: 154 5661
BssSI C'ACGA_G
Cuts at: 2669 3875 4887 2669
Size: 1206 1012 3597
Fragments arranged by size:
3597 1206 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 2599 1184
Size: 1415 4400
BstYI r'GATC_y
Cuts at: 1972 2556 3145 3512 3897 3914 5366 5452 5464
Size: 584 589 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 589 584 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4324
Fragments arranged by size:
4324 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2158 2630 487
Size: 683 56 932 472 3672
Fragments arranged by size:
3672 932 683 472 56
BtsI GCAGTG_nn'
Cuts at: 2432 3331 4125 4145 2432
Size: 899 794 20 4102
Fragments arranged by size:
4102 899 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2621 4822 809
Size: 507 1305 2201 1802
Fragments arranged by size:
2201 1802 1305 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 3262 4151
Size: 54 1425 50 275 18 194 1238 889
Cuts at: 4151 5780 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1238 889 275 194 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3745 1353
Size: 2392 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2120 2149 2502 2510 3878 4475
Size: 393 428 60 29 353 8 1368 597
Cuts at: 4475 5262 5530 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1368 787 597 428 393 353 268
60 29 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2609 2609
Size: 5815
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 5815
EcoRI G'AATT_C
Cuts at: 3010 3124 3502 3010
Size: 114 378 5323
Fragments arranged by size:
5323 378 114
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 2016 2134 3052 4962 1678
Size: 338 118 918 1910 2531
Fragments arranged by size:
2531 1910 918 338 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2258 2290 2688
Size: 32 135 32 473 32 181 32 398
Cuts at: 2688 2720 4456 4488 4530 4562 1373
Size: 32 1736 32 42 32 2626
Fragments arranged by size:
2626 1736 473 398 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KasI G'GCGC_C
Cuts at: 2012 3048 2012
Size: 1036 4779
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2371 2706 2743 4929 5113 1305
Size: 1066 335 37 2186 184 2007
Fragments arranged by size:
2186 2007 1066 335 184 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3993
Fragments arranged by size:
3993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 2990 3773 4132 4291 518
Size: 648 1126 698 783 359 159 2042
Fragments arranged by size:
2042 1126 783 698 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2073 2211 2443 3231 3908 5056 5301
Size: 432 35 138 232 788 677 1148 245
Cuts at: 5301 1606
Size: 2120
Fragments arranged by size:
2120 1148 788 677 432 245 232 138
35
NarI GG'CG_CC
Cuts at: 2013 3049 2013
Size: 1036 4779
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4324
Fragments arranged by size:
4324 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 2995 1052
Size: 1943 3872
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2624 3173 4718 1132
Size: 97 1395 549 1545 2229
Fragments arranged by size:
2229 1545 1395 549 97
PasI CC'CwG_GG
Cuts at: 1348 2505 1348
Size: 1157 4658
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2038 2185 1108
Size: 930 147 4738
Fragments arranged by size:
4738 930 147
PfoI T'CCnGG_A
Cuts at: 2136 2355 2136
Size: 219 5596
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PspOMI G'GGCC_C
Cuts at: 2094 2094
Size: 5815
PstI C_TGCA'G
Cuts at: 830 2613 3167 830
Size: 1783 554 3478
Fragments arranged by size:
3478 1783 554
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2038 2038
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 2613 3167 2613
Size: 554 5261
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SexAI A'CCwGG_T
Cuts at: 2184 2184
Size: 5815
SfcI C'TryA_G
Cuts at: 826 1045 2609 3163 4298 4979 5170 826
Size: 219 1564 554 1135 681 191 1471
Fragments arranged by size:
1564 1471 1135 681 554 219 191
SfoI GGC'GCC
Cuts at: 2014 3050 2014
Size: 1036 4779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3919 4820 5082 820
Size: 197 926 1976 901 262 1553
Fragments arranged by size:
1976 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 3739 5
Size: 47 3687 2081
Fragments arranged by size:
3687 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 3254 513
Size: 552 939 1250 3074
Fragments arranged by size:
3074 1250 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 3842 3859 4012 4197 4642 325
Size: 1255 906 1356 17 153 185 445 1498
Fragments arranged by size:
1498 1356 1255 906 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 2782 3151 2782
Size: 369 5446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 2339 3944 2339
Size: 1605 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI XcmI