Restriction map for
pFN21AB7916
(Circular) (Six-base) MAPSORT of: pFN21AB7916.seq Check: 8970 from: 1 to: 5740
pFN21AB7916 5740 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2949 3550 278
Size: 53 83 186 936 1413 601 2468
Fragments arranged by size:
2468 1413 936 601 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2775 3083 1937
Size: 838 308 4594
Fragments arranged by size:
4594 838 308
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2343 3803 5187 5455 1632
Size: 711 1460 1384 268 1917
Fragments arranged by size:
1917 1460 1384 711 268
AfeI AGC'GCT
Cuts at: 2839 2839
Size: 5740
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 2740 4571 2740
Size: 1831 3909
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaI G_GGCC'C
Cuts at: 2331 2331
Size: 5740
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 3049 3275 3427 5536 1493
Size: 1556 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2433 3065 1870
Size: 73 253 237 632 4545
Fragments arranged by size:
4545 632 253 237 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2235 2268 2235
Size: 33 5707
BamHI G'GATC_C
Cuts at: 2169 3070 3437 2169
Size: 901 367 4472
Fragments arranged by size:
4472 901 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3018 3061 4516 618
Size: 325 217 627 1231 43 1455 1842
Fragments arranged by size:
1842 1455 1231 627 325 217 43
BanII G_rGCy'C
Cuts at: 1391 2196 2331 2479 2757 2877 3059 1391
Size: 805 135 148 278 120 182 4072
Fragments arranged by size:
4072 805 278 182 148 135 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 928
Size: 971 333 4436
Fragments arranged by size:
4436 971 333
BbvCI CC'TCA_GC
Cuts at: 2549 2549
Size: 5740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2168 2202 3931 3965 1903
Size: 34 50 34 147 34 1729 34 3678
Fragments arranged by size:
3678 1729 147 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2516 2996 3027
Size: 107 122 71 631 605 844 480 31
Cuts at: 3027 4351 136
Size: 1324 1525
Fragments arranged by size:
1525 1324 844 631 605 480 122 107
71 31
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2331 3801 4957 1790
Size: 541 1470 1156 2573
Fragments arranged by size:
2573 1470 1156 541
BmgBI CAC'GTC
Cuts at: 1318 2810 1318
Size: 1492 4248
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2825 3471 4429
Size: 416 53 83 399 24 1408 646 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1408 958 646 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2739 2739
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2230 4400 1239
Size: 991 2170 2579
Fragments arranged by size:
2579 2170 991
Bpu10I CC'TnA_GC
Cuts at: 2549 2549
Size: 5740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2295 4403 882
Size: 617 796 2108 2219
Fragments arranged by size:
2219 2108 796 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2946 3547 3929 4564
Size: 53 83 186 936 1413 601 382 635
Cuts at: 4564 275
Size: 1451
Fragments arranged by size:
1451 1413 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2740 4173 4571 4845 4992 5403 2740
Size: 1433 398 274 147 411 3077
Fragments arranged by size:
3077 1433 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5740
BseYI C'CCAG_C
Cuts at: 2142 2565 4943 5439 2142
Size: 423 2378 496 2443
Fragments arranged by size:
2443 2378 496 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2074 2901 1163
Size: 573 338 827 4002
Fragments arranged by size:
4002 827 573 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3801 3886 4957 3801
Size: 85 1071 4584
Fragments arranged by size:
4584 1071 85
BsmI GAATG_Cn'
Cuts at: 3030 3251 3344 3030
Size: 221 93 5426
Fragments arranged by size:
5426 221 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2331 2479 2757 2877 3059 3801
Size: 399 406 135 148 278 120 182 742
Cuts at: 3801 3886 4957 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 278 182 148
135 120 85
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3095 844
Size: 2251 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2740 4384 4571 1700
Size: 110 930 1644 187 2869
Fragments arranged by size:
2869 1644 930 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2169 3070 3437 3822 3839 5291 5377 5389
Size: 197 901 367 385 17 1452 86 12
Cuts at: 5389 5735 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 901 385 367 346 197 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2388 2388
Size: 5740
BtgI C'CryG_G
Cuts at: 513 1065 2414 2844 513
Size: 552 1349 430 3409
Fragments arranged by size:
3409 1349 552 430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DraIII CAC_nnn'GTG
Cuts at: 2692 2692
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2213 2814 4747 809
Size: 507 897 601 1933 1802
Fragments arranged by size:
1933 1802 897 601 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2812 3187 4076
Size: 54 1425 50 275 18 982 375 889
Cuts at: 4076 5705 8
Size: 1629 43
Fragments arranged by size:
1629 1425 982 889 375 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2904 3670 1353
Size: 1551 766 3423
Fragments arranged by size:
3423 1551 766
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2714 4327 4701 4847 387
Size: 1064 138 1125 1613 374 146 1280
Fragments arranged by size:
1613 1280 1125 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2343 3803 4400 5187 5455 1239
Size: 393 598 113 1460 597 787 268 1524
Fragments arranged by size:
1524 1460 787 598 597 393 268 113
EcoNI CCTnn'n_nnAGG
Cuts at: 2903 2903
Size: 5740
EcoO109I rG'GnC_Cy
Cuts at: 2327 2328 2327
Size: 1 5739
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 2030 4246 2030
Size: 2216 3524
HaeII r_GCGC'y
Cuts at: 1678 2841 4887 1678
Size: 1163 2046 2531
Fragments arranged by size:
2531 2046 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373
Size: 32 135 32 2809 32 42 32 2626
Fragments arranged by size:
2809 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 2287 2392 4854 5038 1305
Size: 739 243 105 2462 184 2007
Fragments arranged by size:
2462 2007 739 243 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3698 4057 4216 518
Size: 648 2532 359 159 2042
Fragments arranged by size:
2532 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2485 3156 3833 4981 5226 1606
Size: 879 671 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 671 245
NcoI C'CATG_G
Cuts at: 513 1065 2844 513
Size: 552 1779 3409
Fragments arranged by size:
3409 1779 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 2189 3098 4643 1132
Size: 97 960 909 1545 2229
Fragments arranged by size:
2229 1545 960 909 97
PasI CC'CwG_GG
Cuts at: 1348 2470 3014 1348
Size: 1122 544 4074
Fragments arranged by size:
4074 1122 544
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PspOMI G'GGCC_C
Cuts at: 2327 2327
Size: 5740
PstI C_TGCA'G
Cuts at: 830 2860 2978 3092 830
Size: 2030 118 114 3478
Fragments arranged by size:
3478 2030 118 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2856 2974 3088 4223 4904 5095 826
Size: 219 1811 118 114 1135 681 191 1471
Fragments arranged by size:
1811 1471 1135 681 219 191 118 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 2198 2435 3067 1872
Size: 326 237 632 4545
Fragments arranged by size:
4545 632 326 237
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 2189 3098 1132
Size: 1057 909 3774
Fragments arranged by size:
3774 1057 909
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2239 2514 2844 3179 513
Size: 552 1174 275 330 335 3074
Fragments arranged by size:
3074 1174 552 335 330 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 3784 3937 4122 4567 325
Size: 1255 2187 17 153 185 445 1498
Fragments arranged by size:
2187 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3986 5698 96
Size: 274 80 33 51 1443 2009 1712 138
Fragments arranged by size:
2009 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 2196 2433 3065 1870
Size: 326 237 632 4545
Fragments arranged by size:
4545 632 326 237
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2947 3548 276
Size: 53 83 186 936 1413 601 2468
Fragments arranged by size:
2468 1413 936 601 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI