Restriction map for
pFN21AB7905
(Circular) (Six-base) MAPSORT of: pFN21AB7905.seq Check: 1681 from: 1 to: 5077
pFN21AB7905 5077 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2887 278
Size: 53 83 186 936 1351 2468
Fragments arranged by size:
2468 1351 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2420 1937
Size: 483 4594
Acc65I G'GTAC_C
Cuts at: 2398 2398
Size: 5077
AclI AA'CG_TT
Cuts at: 1563 3204 3577 1563
Size: 1641 373 3063
Fragments arranged by size:
3063 1641 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3140 4524 4792 1632
Size: 1508 1384 268 1917
Fragments arranged by size:
1917 1508 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4880
AflIII A'CryG_T
Cuts at: 1203 3976 4953 1203
Size: 2773 977 1327
Fragments arranged by size:
2773 1327 977
AgeI A'CCGG_T
Cuts at: 3908 3908
Size: 5077
AhdI GACnn_n'nnGTC
Cuts at: 3806 3806
Size: 5077
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5077
AlwNI CAG_nnn'CTG
Cuts at: 2820 4392 2820
Size: 1572 3505
ApaLI G'TGCA_C
Cuts at: 3134 4290 3134
Size: 1156 3921
ApoI r'AATT_y
Cuts at: 1493 2386 2612 2764 4873 1493
Size: 893 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3631 160
Size: 872 2599 1606
Fragments arranged by size:
2599 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5077
AvaI C'yCGr_G
Cuts at: 1870 1943 2402 1870
Size: 73 459 4545
Fragments arranged by size:
4545 459 73
BamHI G'GATC_C
Cuts at: 2407 2774 2407
Size: 367 4710
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 3853 618
Size: 325 217 627 611 1455 1842
Fragments arranged by size:
1842 1455 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2396 1391
Size: 1005 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2239 2257 928
Size: 971 129 211 18 3748
Fragments arranged by size:
3748 971 211 129 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3268 3302 1903
Size: 34 1331 34 3678
Fragments arranged by size:
3678 1331 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2969 4179 2969
Size: 1210 3867
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5077
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3688 136
Size: 107 122 71 631 605 2016 1525
Fragments arranged by size:
2016 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2179 5072 2179
Size: 2893 2184
BlpI GC'TnA_GC
Cuts at: 2494 2494
Size: 5077
Bme1580I G_kGCm'C
Cuts at: 1790 3138 4294 1790
Size: 1348 1156 2573
Fragments arranged by size:
2573 1348 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5077
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2808 3766 442
Size: 416 53 83 399 24 1391 958 1753
Fragments arranged by size:
1753 1391 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3737 1239
Size: 2498 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3202 4067 4365 3202
Size: 865 298 3914
Fragments arranged by size:
3914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3740 882
Size: 617 2241 2219
Fragments arranged by size:
2241 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2868 493
Size: 711 1664 2702
Fragments arranged by size:
2702 1664 711
BsaBI GATnn'nnATC
Cuts at: 5071 5071
Size: 5077
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2884 3266 3901 275
Size: 53 83 186 936 1351 382 635 1451
Fragments arranged by size:
1451 1351 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3510 3908 4182 4329 4740 3510
Size: 398 274 147 411 3847
Fragments arranged by size:
3847 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4852
Fragments arranged by size:
4852 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2073 1186
Size: 887 4190
BseYI C'CCAG_C
Cuts at: 4280 4776 4280
Size: 496 4581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2085 1163
Size: 573 349 4155
Fragments arranged by size:
4155 573 349
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2527 3288 4316 1540
Size: 275 186 526 761 1028 2301
Fragments arranged by size:
2301 1028 761 526 275 186
BsiHKAI G_wGCw'C
Cuts at: 3138 3223 4294 3138
Size: 85 1071 3921
Fragments arranged by size:
3921 1071 85
BsmI GAATG_Cn'
Cuts at: 2588 2681 2588
Size: 93 4984
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 3138 3223 4294 1391
Size: 399 606 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 399 85
BspEI T'CCGG_A
Cuts at: 4740 4740
Size: 5077
BspHI T'CATG_A
Cuts at: 2966 4696 2966
Size: 1730 3347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 844
Size: 1588 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2964 1394
Size: 429 1141 3507
Fragments arranged by size:
3507 1141 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2226 3572 3746 65
Size: 1183 978 1346 174 1396
Fragments arranged by size:
1396 1346 1183 978 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3721 3908 1700
Size: 110 1911 187 2869
Fragments arranged by size:
2869 1911 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5077
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5077
BssSI C'ACGA_G
Cuts at: 3137 4149 3137
Size: 1012 4065
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5077
BstBI TT'CG_AA
Cuts at: 2275 3881 2275
Size: 1606 3471
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5077
BstYI r'GATC_y
Cuts at: 1972 2179 2372 2407 2774 3159 3176 4628 4714
Size: 207 193 35 367 385 17 1452 86
Cuts at: 4714 4726 5072 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 207 193 86
35 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3586
Fragments arranged by size:
3586 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 487
Size: 683 56 902 3436
Fragments arranged by size:
3436 902 683 56
BtsI GCAGTG_nn'
Cuts at: 2593 3387 3407 2593
Size: 794 20 4263
Fragments arranged by size:
4263 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5042
DraI TTT'AAA
Cuts at: 2381 2381
Size: 5077
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4084 809
Size: 507 2768 1802
Fragments arranged by size:
2768 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2524 3413 5042
Size: 54 1425 50 275 18 694 889 1629
Cuts at: 5042 8
Size: 43
Fragments arranged by size:
1629 1425 889 694 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2524 1537
Size: 275 712 4090
Fragments arranged by size:
4090 712 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3007 1353
Size: 1654 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3664 4038 4184 387
Size: 1064 138 2075 374 146 1280
Fragments arranged by size:
2075 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3140 3737 4524 4792 1239
Size: 393 1508 597 787 268 1524
Fragments arranged by size:
1524 1508 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2281 2281
Size: 5077
EcoRI G'AATT_C
Cuts at: 2386 2764 2386
Size: 378 4699
EcoRV GAT'ATC
Cuts at: 2260 2260
Size: 5077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2330 2330
Size: 5077
FspI TGC'GCA
Cuts at: 3583 3583
Size: 5077
HaeII r_GCGC'y
Cuts at: 1678 4224 1678
Size: 2546 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3718 3750 3792 3824 1373
Size: 32 135 32 2146 32 42 32 2626
Fragments arranged by size:
2626 2146 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 2667 669
Size: 988 281 483 246 3079
Fragments arranged by size:
3079 988 483 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5077
HpaI GTT'AAC
Cuts at: 2667 2667
Size: 5077
KpnI G_GTAC'C
Cuts at: 2402 2402
Size: 5077
MfeI C'AATT_G
Cuts at: 2226 2676 2226
Size: 450 4627
MluI A'CGCG_T
Cuts at: 4953 4953
Size: 5077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4191 4375 1305
Size: 2886 184 2007
Fragments arranged by size:
2886 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3255
Fragments arranged by size:
3255 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 3035 3394 3553 518
Size: 648 1038 831 359 159 2042
Fragments arranged by size:
2042 1038 831 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2493 3170 4318 4563 1606
Size: 887 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3586
Fragments arranged by size:
3586 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5077
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5077
NotI GC'GGCC_GC
Cuts at: 2524 2524
Size: 5077
NspI r_CATG'y
Cuts at: 1132 1229 2435 3980 1132
Size: 97 1206 1545 2229
Fragments arranged by size:
2229 1545 1206 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5077
PciI A'CATG_T
Cuts at: 3976 3976
Size: 5077
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5077
PmeI GTTT'AAAC
Cuts at: 2381 2381
Size: 5077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 4659 4691 3146
Size: 32 1481 32 3532
Fragments arranged by size:
3532 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2281
Size: 5077
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5077
PsiI TTA'TAA
Cuts at: 2647 2647
Size: 5077
PstI C_TGCA'G
Cuts at: 830 2429 830
Size: 1599 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5077
SalI G'TCGA_C
Cuts at: 1936 2419 1936
Size: 483 4594
SbfI CC_TGCA'GG
Cuts at: 2429 2429
Size: 5077
ScaI AGT'ACT
Cuts at: 3325 5037 3325
Size: 1712 3365
SfcI C'TryA_G
Cuts at: 826 1045 2425 3560 4241 4432 826
Size: 219 1380 1135 681 191 1471
Fragments arranged by size:
1471 1380 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5077
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5077
SmaI CCC'GGG
Cuts at: 1872 2404 1872
Size: 532 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3181 4082 4344 820
Size: 197 926 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2868 493
Size: 2375 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5077
SphI G_CATG'C
Cuts at: 1132 2435 1132
Size: 1303 3774
SspI AAT'ATT
Cuts at: 5 52 3001 5
Size: 47 2949 2081
Fragments arranged by size:
2949 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5077
StyI C'CwwG_G
Cuts at: 513 1065 2004 2285 2516 513
Size: 552 939 281 231 3074
Fragments arranged by size:
3074 939 552 281 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3104 3121 3274 3459 3904 325
Size: 1255 1524 17 153 185 445 1498
Fragments arranged by size:
1524 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2245 3323 5035
Size: 274 80 33 51 1443 268 1078 1712
Cuts at: 5035 96
Size: 138
Fragments arranged by size:
1712 1443 1078 274 268 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5037 5069 5037
Size: 32 5045
Tth111I GACn'n_nGTC
Cuts at: 1316 2238 1316
Size: 922 4155
XbaI T'CTAG_A
Cuts at: 2413 2413
Size: 5077
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5077
XmaI C'CCGG_G
Cuts at: 1870 2402 1870
Size: 532 4545
XmnI GAAnn'nnTTC
Cuts at: 3206 3206
Size: 5077
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2885 276
Size: 53 83 186 936 1351 2468
Fragments arranged by size:
2468 1351 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI