Restriction map for
pFN21AB7895
(Circular) (Six-base) MAPSORT of: pFN21AB7895.seq Check: 3232 from: 1 to: 6469
pFN21AB7895 6469 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3318 3812 1937
Size: 1381 494 4594
Fragments arranged by size:
4594 1381 494
Acc65I G'GTAC_C
Cuts at: 3213 3790 3213
Size: 577 5892
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2916 3465 3470 4532 5916 6184 1632
Size: 695 589 549 5 1062 1384 268 1917
Fragments arranged by size:
1917 1384 1062 695 589 549 268 5
AfeI AGC'GCT
Cuts at: 2336 2336
Size: 6469
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 3464 3580 3766 5368 6345 1203
Size: 2261 116 186 1602 977 1327
Fragments arranged by size:
2261 1602 1327 977 186 116
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6469
AlwNI CAG_nnn'CTG
Cuts at: 3401 4212 5784 3401
Size: 811 1572 4086
Fragments arranged by size:
4086 1572 811
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 3529 3557 3778 4004 4156 6265 1493
Size: 2036 28 221 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 221 152 28
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 3794 1870
Size: 73 69 1782 4545
Fragments arranged by size:
4545 1782 73 69
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3213 3790 5245 618
Size: 325 217 627 1426 577 1455 1842
Fragments arranged by size:
1842 1455 1426 627 577 325 217
BanII G_rGCy'C
Cuts at: 1391 3788 1391
Size: 2397 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2904 928
Size: 971 1005 4493
Fragments arranged by size:
4493 1005 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3367 3401 4660 4694 1903
Size: 34 1430 34 1259 34 3678
Fragments arranged by size:
3678 1430 1259 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BfrBI ATG'CAT
Cuts at: 2968 2968
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3047 5080 136
Size: 107 122 71 631 605 1375 2033 1525
Fragments arranged by size:
2033 1525 1375 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2701 6464 2701
Size: 3763 2706
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 4530 5686 1790
Size: 2740 1156 2573
Fragments arranged by size:
2740 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2786 1318
Size: 1468 5001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 2443 4200
Size: 416 53 83 399 24 952 74 1757
Cuts at: 4200 5158 442
Size: 958 1753
Fragments arranged by size:
1757 1753 958 952 416 399 83 74
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5129 1239
Size: 3890 2579
Bpu10I CC'TnA_GC
Cuts at: 2448 2448
Size: 6469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4594 5459 5757 4594
Size: 865 298 5306
Fragments arranged by size:
5306 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5132 882
Size: 617 3633 2219
Fragments arranged by size:
3633 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2986 4260 493
Size: 711 1782 1274 2702
Fragments arranged by size:
2702 1782 1274 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4276 4658 5293 275
Size: 53 83 186 936 2743 382 635 1451
Fragments arranged by size:
2743 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4902 5300 5574 5721 6132 4902
Size: 398 274 147 411 5239
Fragments arranged by size:
5239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 1178
Size: 30 165 30 1086 30 5128
Fragments arranged by size:
5128 1086 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 5625
BseYI C'CCAG_C
Cuts at: 2217 5672 6168 2217
Size: 3455 496 2518
Fragments arranged by size:
3455 2518 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5896
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4530 4615 5686 4530
Size: 85 1071 5313
Fragments arranged by size:
5313 1071 85
BsmI GAATG_Cn'
Cuts at: 3980 4073 3980
Size: 93 6376
BsmBI CGTCTCn'nnnn_
Cuts at: 3754 3754
Size: 6469
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3788 4530 4615 5686 1391
Size: 399 1998 742 85 1071 2174
Fragments arranged by size:
2174 1998 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3824 844
Size: 2980 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2495 4356 1394
Size: 429 672 1861 3507
Fragments arranged by size:
3507 1861 672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4964 5138 65
Size: 1183 3716 174 1396
Fragments arranged by size:
3716 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5113 5300 1700
Size: 110 3303 187 2869
Fragments arranged by size:
3303 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3070 3575 96
Size: 2974 505 2990
Fragments arranged by size:
2990 2974 505
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2103 794
Size: 1309 5160
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 2576 2701 3746 3799 4166 4551 4568 6020
Size: 604 125 1045 53 367 385 17 1452
Cuts at: 6020 6106 6118 6464 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1045 604 385 367 346 125
86 53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3191 3472 513
Size: 552 939 1187 281 3510
Fragments arranged by size:
3510 1187 939 552 281
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5730
Fragments arranged by size:
5730 683 56
BtsI GCAGTG_nn'
Cuts at: 3515 3985 4779 4799 3515
Size: 470 794 20 5185
Fragments arranged by size:
5185 794 470 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 2032 3773 2032
Size: 1741 4728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3916 4805 6434
Size: 54 1425 50 275 18 2086 889 1629
Cuts at: 6434 8
Size: 43
Fragments arranged by size:
2086 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2342 2715 3441 3644 4399 1353
Size: 666 323 373 726 203 755 3423
Fragments arranged by size:
3423 755 726 666 373 323 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 5056 5430 5576 387
Size: 1064 138 447 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 447 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2916 3465 3470 4532 5129 5916
Size: 393 695 589 549 5 1062 597 787
Cuts at: 5916 6184 1239
Size: 268 1524
Fragments arranged by size:
1524 1062 787 695 597 589 549 393
268 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2015
Size: 6469
EcoRI G'AATT_C
Cuts at: 3529 3778 4156 3529
Size: 249 378 5842
Fragments arranged by size:
5842 378 249
EcoRV GAT'ATC
Cuts at: 2126 2126
Size: 6469
FspI TGC'GCA
Cuts at: 4975 4975
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 2338 5616 1678
Size: 660 3278 2531
Fragments arranged by size:
3278 2531 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2489 2521 3016 3048 5110
Size: 32 135 32 917 32 495 32 2062
Cuts at: 5110 5142 5184 5216 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2062 917 495 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2950 3319 3813 4059 669
Size: 988 281 1012 369 494 246 3079
Fragments arranged by size:
3079 1012 988 494 369 281 246
HindIII A'AGCT_T
Cuts at: 748 3231 748
Size: 2483 3986
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KpnI G_GTAC'C
Cuts at: 3217 3794 3217
Size: 577 5892
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2836 5583 5767 1305
Size: 1531 2747 184 2007
Fragments arranged by size:
2747 2007 1531 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4647
Fragments arranged by size:
4647 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 4427 4786 4945 518
Size: 648 1926 1335 359 159 2042
Fragments arranged by size:
2042 1926 1335 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2469 3885 4562 5710 5955 1606
Size: 863 1416 677 1148 245 2120
Fragments arranged by size:
2120 1416 1148 863 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3191 3472 513
Size: 552 939 1187 281 3510
Fragments arranged by size:
3510 1187 939 552 281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NsiI A_TGCA'T
Cuts at: 2970 2970
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 3584 3770 3827 5372 1132
Size: 97 2355 186 57 1545 2229
Fragments arranged by size:
2355 2229 1545 186 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 3580 3766 5368 3580
Size: 186 1602 4681
Fragments arranged by size:
4681 1602 186
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PmlI CAC'GTG
Cuts at: 2986 2986
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PspXI vC'TCGA_Gb
Cuts at: 2012 2012
Size: 6469
PstI C_TGCA'G
Cuts at: 830 3566 3821 830
Size: 2736 255 3478
Fragments arranged by size:
3478 2736 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 2469 2469
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3317 3811 1936
Size: 1381 494 4594
Fragments arranged by size:
4594 1381 494
SapI GCTCTTCn'nnn_
Cuts at: 2342 2342
Size: 6469
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 4717 6429 4717
Size: 1712 4757
SfcI C'TryA_G
Cuts at: 826 1045 3036 3078 3362 3562 3817 4952 5633
Size: 219 1991 42 284 200 255 1135 681
Cuts at: 5633 5824 826
Size: 191 1471
Fragments arranged by size:
1991 1471 1135 681 284 255 219 200
191 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 4573 5474 5736 820
Size: 197 926 69 2561 901 262 1553
Fragments arranged by size:
2561 1553 926 901 262 197 69
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 2090 4393 5
Size: 47 2038 2303 2081
Fragments arranged by size:
2303 2081 2038 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 2004 2284 3191 3472 3908 513
Size: 552 939 280 907 281 436 3074
Fragments arranged by size:
3074 939 907 552 436 281 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4496 4513 4666 4851 5296 325
Size: 1255 2916 17 153 185 445 1498
Fragments arranged by size:
2916 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2042 3070 3548
Size: 274 80 33 51 1443 65 1028 478
Cuts at: 3548 3575 4715 6427 96
Size: 27 1140 1712 138
Fragments arranged by size:
1712 1443 1140 1028 478 274 138 80
65 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6429 6461 6429
Size: 32 6437
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 2012 1943
Size: 69 6400
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 4598 4598
Size: 6469
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PpuMI PspOMI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI