Restriction map for
pFN21AB7893
(Circular) (Six-base) MAPSORT of: pFN21AB7893.seq Check: 728 from: 1 to: 6469
pFN21AB7893 6469 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3812 1937
Size: 1875 4594
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 2269 4532 5916 6184 1632
Size: 572 65 2263 1384 268 1917
Fragments arranged by size:
2263 1917 1384 572 268 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6469
AlwNI CAG_nnn'CTG
Cuts at: 4212 5784 4212
Size: 1572 4897
ApaI G_GGCC'C
Cuts at: 2078 2078
Size: 6469
ApaLI G'TGCA_C
Cuts at: 3393 4526 5682 3393
Size: 1133 1156 4180
Fragments arranged by size:
4180 1156 1133
ApoI r'AATT_y
Cuts at: 1493 3140 3505 3778 4004 4156 6265 1493
Size: 1647 365 273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1647 365 273 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2438 2466 2645 3093 3546 3794 1870
Size: 73 495 28 179 448 453 248 4545
Fragments arranged by size:
4545 495 453 448 248 179 73 28
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2618 3524 3790 5245
Size: 325 217 627 312 519 906 266 1455
Cuts at: 5245 618
Size: 1842
Fragments arranged by size:
1842 1455 906 627 519 325 312 266
217
BanII G_rGCy'C
Cuts at: 1391 2045 2069 2078 2331 2439 2652 2706 3788
Size: 654 24 9 253 108 213 54 1082
Cuts at: 3788 1391
Size: 4072
Fragments arranged by size:
4072 1082 654 253 213 108 54 24
9
BbeI G_GCGC'C
Cuts at: 2622 2622
Size: 6469
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5498
BbvCI CC'TCA_GC
Cuts at: 2157 2157
Size: 6469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4660 4694 1903
Size: 34 2723 34 3678
Fragments arranged by size:
3678 2723 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 3039 1548
Size: 1491 4978
BfrBI ATG'CAT
Cuts at: 3264 3264
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2073 2082 5080
Size: 107 122 71 631 605 401 9 2998
Cuts at: 5080 136
Size: 1525
Fragments arranged by size:
2998 1525 631 605 401 122 107 71
9
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2142 3397 4530 5686 1790
Size: 288 64 1255 1133 1156 2573
Fragments arranged by size:
2573 1255 1156 1133 288 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6469
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2073 3761 4200
Size: 416 53 83 399 24 656 1688 439
Cuts at: 4200 5158 442
Size: 958 1753
Fragments arranged by size:
1753 1688 958 656 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2008 2111 2315 2633 2954 2987 3020 3224
Size: 769 103 204 318 321 33 33 204
Cuts at: 3224 3550 5129 1239
Size: 326 1579 2579
Fragments arranged by size:
2579 1579 769 326 321 318 204 204
103 33 33
Bpu10I CC'TnA_GC
Cuts at: 2157 3299 2157
Size: 1142 5327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2828 4594 5459 5757 2585
Size: 243 1766 865 298 3297
Fragments arranged by size:
3297 1766 865 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 2625 3485 5132 882
Size: 617 939 187 860 1647 2219
Fragments arranged by size:
2219 1647 939 860 617 187
BsaAI yAC'GTr
Cuts at: 493 1204 4260 493
Size: 711 3056 2702
Fragments arranged by size:
3056 2702 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2619 4276 4658 5293
Size: 53 83 186 936 1086 1657 382 635
Cuts at: 5293 275
Size: 1451
Fragments arranged by size:
1657 1451 1086 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3397 3447 4902 5300 5574 5721 6132 3397
Size: 50 1455 398 274 147 411 3734
Fragments arranged by size:
3734 1455 411 398 274 147 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2504 2534 3527
Size: 30 165 30 855 30 216 30 993
Cuts at: 3527 3557 1178
Size: 30 4090
Fragments arranged by size:
4090 993 855 216 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 2801 3553 3604 1186
Size: 1339 276 752 51 4051
Fragments arranged by size:
4051 1339 752 276 51
BseYI C'CCAG_C
Cuts at: 2335 3669 5672 6168 2335
Size: 1334 2003 496 2636
Fragments arranged by size:
2636 2003 1334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5896
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2706 2760 3397 4530 4615 5686 2706
Size: 54 637 1133 85 1071 3489
Fragments arranged by size:
3489 1133 1071 637 85 54
BsiWI C'GTAC_G
Cuts at: 2319 2319
Size: 6469
BsmI GAATG_Cn'
Cuts at: 3570 3980 4073 3570
Size: 410 93 5966
Fragments arranged by size:
5966 410 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2801 3474 2801
Size: 673 5796
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2069 2078 2142 2331 2439 2652
Size: 399 255 24 9 64 189 108 213
Cuts at: 2652 2706 2760 3397 3788 4530 4615 5686 1391
Size: 54 54 637 391 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 399 391 255 213
189 108 85 64 54 54 24 9
BspEI T'CCGG_A
Cuts at: 3447 6132 3447
Size: 2685 3784
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 3258 3600 3824 844
Size: 1891 523 342 224 3489
Fragments arranged by size:
3489 1891 523 342 224
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2227 4964 5138 65
Size: 1183 979 2737 174 1396
Fragments arranged by size:
2737 1396 1183 979 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3717 5113 5300 1700
Size: 110 1907 1396 187 2869
Fragments arranged by size:
2869 1907 1396 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3703 794
Size: 2909 3560
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 3341 1184
Size: 2157 4312
BstYI r'GATC_y
Cuts at: 1972 3799 4166 4551 4568 6020 6106 6118 6464
Size: 1827 367 385 17 1452 86 12 346
Cuts at: 6464 1972
Size: 1977
Fragments arranged by size:
1977 1827 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 4564
Fragments arranged by size:
4564 939 552 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3332 487
Size: 683 56 2106 3624
Fragments arranged by size:
3624 2106 683 56
BtsI GCAGTG_nn'
Cuts at: 2819 3985 4779 4799 2819
Size: 1166 794 20 4489
Fragments arranged by size:
4489 1166 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 3773 3773
Size: 6469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2815 3596 3706 5476 809
Size: 507 1499 781 110 1770 1802
Fragments arranged by size:
1802 1770 1499 781 507 110
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3475 3719 3916
Size: 54 1425 50 275 18 1645 244 197
Cuts at: 3916 4805 6434 8
Size: 889 1629 43
Fragments arranged by size:
1645 1629 1425 889 275 244 197 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2711 3404 4399 1353
Size: 1358 693 995 3423
Fragments arranged by size:
3423 1358 995 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2467 3308 5056 5430 5576 387
Size: 1064 138 878 841 1748 374 146 1280
Fragments arranged by size:
1748 1280 1064 878 841 374 146 138
EcoICRI GAG'CTC
Cuts at: 2704 2704
Size: 6469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2111 2204 2269 2315 2633 2954
Size: 393 376 103 93 65 46 318 321
Cuts at: 2954 2987 3020 3224 3550 4532 5129 5916 6184
Size: 33 33 204 326 982 597 787 268
Cuts at: 6184 1239
Size: 1524
Fragments arranged by size:
1524 982 787 597 393 376 326 321
318 268 204 103 93 65 46 33
33
EcoNI CCTnn'n_nnAGG
Cuts at: 3203 3203
Size: 6469
EcoO109I rG'GnC_Cy
Cuts at: 2075 2950 2962 3214 2075
Size: 875 12 252 5330
Fragments arranged by size:
5330 875 252 12
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FspI TGC'GCA
Cuts at: 2387 4975 2387
Size: 2588 3881
HaeII r_GCGC'y
Cuts at: 1678 2181 2208 2622 5616 1678
Size: 503 27 414 2994 2531
Fragments arranged by size:
2994 2531 503 414 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2226 2258 2290 5110 5142
Size: 32 135 32 654 32 32 2820 32
Cuts at: 5142 5184 5216 1373
Size: 42 32 2626
Fragments arranged by size:
2820 2626 654 135 42 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3733 3813 4059 669
Size: 988 281 1795 80 246 3079
Fragments arranged by size:
3079 1795 988 281 246 80
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6469
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KasI G'GCGC_C
Cuts at: 2618 2618
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 3780 5583 5767 1305
Size: 996 1479 1803 184 2007
Fragments arranged by size:
2007 1803 1479 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4647
Fragments arranged by size:
4647 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3351 4427 4786 4945 518
Size: 648 2185 1076 359 159 2042
Fragments arranged by size:
2185 2042 1076 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2335 2641 2836 3007 3103 3885 4562
Size: 550 179 306 195 171 96 782 677
Cuts at: 4562 5710 5955 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 782 677 550 306 245 195
179 171 96
NarI GG'CG_CC
Cuts at: 2619 2619
Size: 6469
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4978
Fragments arranged by size:
4978 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NsiI A_TGCA'T
Cuts at: 3266 3266
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 2749 3268 3827 5372 1132
Size: 97 1520 519 559 1545 2229
Fragments arranged by size:
2229 1545 1520 559 519 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PfoI T'CCnGG_A
Cuts at: 2024 2408 2655 2751 2931 2946 2024
Size: 384 247 96 180 15 5547
Fragments arranged by size:
5547 384 247 180 96 15
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2950 2962 2950
Size: 12 6457
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PspOMI G'GGCC_C
Cuts at: 2074 2074
Size: 6469
PspXI vC'TCGA_Gb
Cuts at: 2645 2645
Size: 6469
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2264 2296 2264
Size: 32 6437
PstI C_TGCA'G
Cuts at: 830 2017 2841 3138 3821 830
Size: 1187 824 297 683 3478
Fragments arranged by size:
3478 1187 824 683 297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 2156 2335 2641 2836 3103 2156
Size: 179 306 195 267 5522
Fragments arranged by size:
5522 306 267 195 179
SacI G_AGCT'C
Cuts at: 2706 2706
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SapI GCTCTTCn'nnn_
Cuts at: 2711 2711
Size: 6469
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 3560 4717 6429 3560
Size: 1157 1712 3600
Fragments arranged by size:
3600 1712 1157
SexAI A'CCwGG_T
Cuts at: 2952 2952
Size: 6469
SfcI C'TryA_G
Cuts at: 826 1045 2013 2837 3134 3817 4952 5633 5824
Size: 219 968 824 297 683 1135 681 191
Cuts at: 5824 826
Size: 1471
Fragments arranged by size:
1471 1135 968 824 683 681 297 219
191
SfoI GGC'GCC
Cuts at: 2620 2620
Size: 6469
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 2440 2468 3095 3548 3796 1872
Size: 568 28 627 453 248 4545
Fragments arranged by size:
4545 627 568 453 248 28
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 2645 2843 4573 5474 5736
Size: 197 926 657 45 198 1730 901 262
Cuts at: 5736 820
Size: 1553
Fragments arranged by size:
1730 1553 926 901 657 262 198 197
45
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3268 3827 1132
Size: 2136 559 3774
Fragments arranged by size:
3774 2136 559
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 1517
Size: 1062 5407
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 2793 3066 3486 3604 3663
Size: 552 939 99 690 273 420 118 59
Cuts at: 3663 3908 513
Size: 245 3074
Fragments arranged by size:
3074 939 690 552 420 273 245 118
99 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2732 3448 3512 4496 4513 4666 4851
Size: 1255 1152 716 64 984 17 153 185
Cuts at: 4851 5296 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1152 984 716 445 185 153
64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3558 4715 6427
Size: 274 80 33 51 1443 1581 1157 1712
Cuts at: 6427 96
Size: 138
Fragments arranged by size:
1712 1581 1443 1157 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 6429 6461 2504
Size: 32 3893 32 2512
Fragments arranged by size:
3893 2512 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2086
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 2645 1943
Size: 702 5767
XmaI C'CCGG_G
Cuts at: 1870 2438 2466 3093 3546 3794 1870
Size: 568 28 627 453 248 4545
Fragments arranged by size:
4545 627 568 453 248 28
XmnI GAAnn'nnTTC
Cuts at: 3374 3506 4598 3374
Size: 132 1092 5245
Fragments arranged by size:
5245 1092 132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BstXI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PmlI RsrII SacII SanDI SfiI SrfI
SwaI