Restriction map for
pFN21AB7892
(Circular) (Six-base) MAPSORT of: pFN21AB7892.seq Check: 9091 from: 1 to: 6664
pFN21AB7892 6664 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3662 3662
Size: 6664
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4007 1937
Size: 2070 4594
Acc65I G'GTAC_C
Cuts at: 3985 3985
Size: 6664
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 3221 4727 6111 6379 1632
Size: 986 603 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 986 603 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6467
AflIII A'CryG_T
Cuts at: 1203 5563 6540 1203
Size: 4360 977 1327
Fragments arranged by size:
4360 1327 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 3925 1166
Size: 2759 3905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3237 3943 1184
Size: 2053 706 3905
Fragments arranged by size:
3905 2053 706
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2544 2576 2544
Size: 32 6632
AlwNI CAG_nnn'CTG
Cuts at: 2525 3431 3668 3794 4407 5979 2525
Size: 906 237 126 613 1572 3210
Fragments arranged by size:
3210 1572 906 613 237 126
ApaI G_GGCC'C
Cuts at: 3387 3387
Size: 6664
ApaLI G'TGCA_C
Cuts at: 3675 4721 5877 3675
Size: 1046 1156 4462
Fragments arranged by size:
4462 1156 1046
ApoI r'AATT_y
Cuts at: 1493 2164 3134 3598 3973 4199 4351 6460 1493
Size: 671 970 464 375 226 152 2109 1697
Fragments arranged by size:
2109 1697 970 671 464 375 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3170 5218 160
Size: 872 2138 2048 1606
Fragments arranged by size:
2138 2048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 3989 1870
Size: 73 2046 4545
Fragments arranged by size:
4545 2046 73
BamHI G'GATC_C
Cuts at: 3592 3994 4361 3592
Size: 402 367 5895
Fragments arranged by size:
5895 402 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3985 5440 618
Size: 325 217 627 2198 1455 1842
Fragments arranged by size:
2198 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2119 3387 3811 3983 1391
Size: 728 1268 424 172 4072
Fragments arranged by size:
4072 1268 728 424 172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 5071
Fragments arranged by size:
5071 971 622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4855 4889 1903
Size: 34 2918 34 3678
Fragments arranged by size:
3678 2918 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 5766 4556
Size: 1210 5454
BclI T'GATC_A
Cuts at: 1548 2770 2892 1548
Size: 1222 122 5320
Fragments arranged by size:
5320 1222 122
BfrBI ATG'CAT
Cuts at: 2281 3539 2281
Size: 1258 5406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3427 5275 136
Size: 107 122 71 631 605 1755 1848 1525
Fragments arranged by size:
1848 1755 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2294 2367 6659 2294
Size: 73 4292 2299
Fragments arranged by size:
4292 2299 73
BlpI GC'TnA_GC
Cuts at: 2044 4081 2044
Size: 2037 4627
Bme1580I G_kGCm'C
Cuts at: 1790 3387 3679 4725 5881 1790
Size: 1597 292 1046 1156 2573
Fragments arranged by size:
2573 1597 1156 1046 292
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3165 3344 3813
Size: 416 53 83 399 24 1748 179 469
Cuts at: 3813 4395 5353 442
Size: 582 958 1753
Fragments arranged by size:
1753 1748 958 582 469 416 399 179
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2586 1056
Size: 1530 5134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3264 3264
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2667 2970 3408 3489 3798 3900 5324 1239
Size: 1428 303 438 81 309 102 1424 2579
Fragments arranged by size:
2579 1428 1424 438 309 303 102 81
Bpu10I CC'TnA_GC
Cuts at: 3579 3579
Size: 6664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4789 5654 5952 4789
Size: 865 298 5501
Fragments arranged by size:
5501 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2712 5327 882
Size: 617 1213 2615 2219
Fragments arranged by size:
2615 2219 1213 617
BsaAI yAC'GTr
Cuts at: 493 1204 4455 493
Size: 711 3251 2702
Fragments arranged by size:
3251 2702 711
BsaBI GATnn'nnATC
Cuts at: 2214 6658 2214
Size: 4444 2220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4471 4853 5488 275
Size: 53 83 186 936 2938 382 635 1451
Fragments arranged by size:
2938 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5097 5495 5769 5916 6327 5097
Size: 398 274 147 411 5434
Fragments arranged by size:
5434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2011 2041 2968 2998 3793
Size: 30 165 30 608 30 927 30 795
Cuts at: 3793 3823 1178
Size: 30 4019
Fragments arranged by size:
4019 927 795 608 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2094 2232 2370 3483 3555 3801 1186
Size: 908 138 138 1113 72 246 4049
Fragments arranged by size:
4049 1113 908 246 138 138 72
BseYI C'CCAG_C
Cuts at: 3727 5867 6363 3727
Size: 2140 496 4028
Fragments arranged by size:
4028 2140 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3444 3858 3949 1163
Size: 573 1708 414 91 3878
Fragments arranged by size:
3878 1708 573 414 91
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3679 4725 4810 5881 3679
Size: 1046 85 1071 4462
Fragments arranged by size:
4462 1071 1046 85
BsmI GAATG_Cn'
Cuts at: 2925 3906 4175 4268 2925
Size: 981 269 93 5321
Fragments arranged by size:
5321 981 269 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 3387 3679 3811 3983 4725 4810
Size: 399 329 1268 292 132 172 742 85
Cuts at: 4810 5881 1391
Size: 1071 2174
Fragments arranged by size:
2174 1268 1071 742 399 329 292 172
132 85
BspEI T'CCGG_A
Cuts at: 6327 6327
Size: 6664
BspHI T'CATG_A
Cuts at: 2571 4553 6283 2571
Size: 1982 1730 2952
Fragments arranged by size:
2952 1982 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3662 3921 4019 844
Size: 2818 259 98 3489
Fragments arranged by size:
3489 2818 259 98
BsrBI CCG'CTC
Cuts at: 1394 1823 4551 1394
Size: 429 2728 3507
Fragments arranged by size:
3507 2728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5159 5333 65
Size: 1183 3911 174 1396
Fragments arranged by size:
3911 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5308 5495 1700
Size: 110 3498 187 2869
Fragments arranged by size:
3498 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6664
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 4724 5736 4724
Size: 1012 5652
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3668 3794 794
Size: 2874 126 3664
Fragments arranged by size:
3664 2874 126
BstBI TT'CG_AA
Cuts at: 3031 5468 3031
Size: 2437 4227
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6664
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 3218 2749
Size: 469 6195
BstYI r'GATC_y
Cuts at: 1972 2294 2367 3262 3592 3994 4361 4746 4763
Size: 322 73 895 330 402 367 385 17
Cuts at: 4763 6215 6301 6313 6659 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 895 402 385 367 346 330
322 86 73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3379 3388 3406 513
Size: 552 939 1375 9 18 3771
Fragments arranged by size:
3771 1375 939 552 18 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2556 487
Size: 683 56 1330 4595
Fragments arranged by size:
4595 1330 683 56
BtsI GCAGTG_nn'
Cuts at: 2105 4180 4974 4994 2105
Size: 2075 794 20 3775
Fragments arranged by size:
3775 2075 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6629
DraI TTT'AAA
Cuts at: 3968 3968
Size: 6664
DraIII CAC_nnn'GTG
Cuts at: 3122 3122
Size: 6664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3072 5671 809
Size: 507 1756 2599 1802
Fragments arranged by size:
2599 1802 1756 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4111 5000 6629
Size: 54 1425 50 275 18 2281 889 1629
Cuts at: 6629 8
Size: 43
Fragments arranged by size:
2281 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2041 3076 3756 4594 1353
Size: 688 1035 680 838 3423
Fragments arranged by size:
3423 1035 838 688 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5251 5625 5771 387
Size: 1064 138 3662 374 146 1280
Fragments arranged by size:
3662 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 2667 2970 3221 3408 3489 3798
Size: 393 986 49 303 251 187 81 309
Cuts at: 3798 3900 4727 5324 6111 6379 1239
Size: 102 827 597 787 268 1524
Fragments arranged by size:
1524 986 827 787 597 393 309 303
268 251 187 102 81 49
EcoO109I rG'GnC_Cy
Cuts at: 2439 2981 3384 3401 3428 2439
Size: 542 403 17 27 5675
Fragments arranged by size:
5675 542 403 27 17
EcoRI G'AATT_C
Cuts at: 2164 3973 4351 2164
Size: 1809 378 4477
Fragments arranged by size:
4477 1809 378
FspI TGC'GCA
Cuts at: 5170 5170
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 5811 1678
Size: 4133 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2792 2824 2877 2909 3793
Size: 32 135 32 1220 32 53 32 884
Cuts at: 3793 3825 5305 5337 5379 5411 1373
Size: 32 1480 32 42 32 2626
Fragments arranged by size:
2626 1480 1220 884 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2086 2451 2999 4008 4254 669
Size: 988 281 148 365 548 1009 246 3079
Fragments arranged by size:
3079 1009 988 548 365 281 246 148
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6664
HpaI GTT'AAC
Cuts at: 2086 2451 4254 2086
Size: 365 1803 4496
Fragments arranged by size:
4496 1803 365
KpnI G_GTAC'C
Cuts at: 3989 3989
Size: 6664
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2317 3824 5778 5962 1305
Size: 1012 1507 1954 184 2007
Fragments arranged by size:
2007 1954 1507 1012 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4842
Fragments arranged by size:
4842 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 3925 4622 4981 5140 518
Size: 648 1375 1384 697 359 159 2042
Fragments arranged by size:
2042 1384 1375 697 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 3731 4080 4757 5905 6150 1606
Size: 442 1683 349 677 1148 245 2120
Fragments arranged by size:
2120 1683 1148 677 442 349 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3379 3388 3406 513
Size: 552 939 1375 9 18 3771
Fragments arranged by size:
3771 1375 939 552 18 9
NdeI CA'TA_TG
Cuts at: 387 3514 387
Size: 3127 3537
NheI G'CTAG_C
Cuts at: 1052 2582 1052
Size: 1530 5134
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NsiI A_TGCA'T
Cuts at: 2283 3541 2283
Size: 1258 5406
NspI r_CATG'y
Cuts at: 1132 1229 3005 4022 5567 1132
Size: 97 1776 1017 1545 2229
Fragments arranged by size:
2229 1776 1545 1017 97
PasI CC'CwG_GG
Cuts at: 1348 3523 3895 1348
Size: 2175 372 4117
Fragments arranged by size:
4117 2175 372
PciI A'CATG_T
Cuts at: 5563 5563
Size: 6664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2525 2945 3122 3431 3756 1108
Size: 1417 420 177 309 325 4016
Fragments arranged by size:
4016 1417 420 325 309 177
PfoI T'CCnGG_A
Cuts at: 3472 3472
Size: 6664
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 6246 6278 4733
Size: 32 1481 32 5119
Fragments arranged by size:
5119 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2439 2981 2439
Size: 542 6122
PshAI GACnn'nnGTC
Cuts at: 1613 2994 1613
Size: 1381 5283
PsiI TTA'TAA
Cuts at: 3468 4234 3468
Size: 766 5898
PspOMI G'GGCC_C
Cuts at: 3383 3383
Size: 6664
PstI C_TGCA'G
Cuts at: 830 2112 3010 3673 4016 830
Size: 1282 898 663 343 3478
Fragments arranged by size:
3478 1282 898 663 343
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
PvuII CAG'CTG
Cuts at: 2048 3731 2048
Size: 1683 4981
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SbfI CC_TGCA'GG
Cuts at: 3673 4016 3673
Size: 343 6321
ScaI AGT'ACT
Cuts at: 3767 4912 6624 3767
Size: 1145 1712 3807
Fragments arranged by size:
3807 1712 1145
SexAI A'CCwGG_T
Cuts at: 3067 3118 3067
Size: 51 6613
SfcI C'TryA_G
Cuts at: 826 1045 2108 3006 3128 3669 4012 5147 5828
Size: 219 1063 898 122 541 343 1135 681
Cuts at: 5828 6019 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1063 898 681 541 343 219
191 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 3427 3427
Size: 6664
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 3991 1872
Size: 2119 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4768 5669 5931 820
Size: 197 926 2825 901 262 1553
Fragments arranged by size:
2825 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 4022 1132
Size: 2890 3774
SspI AAT'ATT
Cuts at: 5 52 4588 5
Size: 47 4536 2081
Fragments arranged by size:
4536 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6664
StyI C'CwwG_G
Cuts at: 513 1065 2004 2742 3379 3388 3406 3750 4103
Size: 552 939 738 637 9 18 344 353
Cuts at: 4103 513
Size: 3074
Fragments arranged by size:
3074 939 738 637 552 353 344 18
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3006 4691 4708 4861 5046 5491 325
Size: 1255 1426 1685 17 153 185 445 1498
Fragments arranged by size:
1685 1498 1426 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3765 4910 6622
Size: 274 80 33 51 1443 1788 1145 1712
Cuts at: 6622 96
Size: 138
Fragments arranged by size:
1788 1712 1443 1145 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6624 6656 6624
Size: 32 6632
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 3386 3413 2540
Size: 846 27 5791
Fragments arranged by size:
5791 846 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6664
XmaI C'CCGG_G
Cuts at: 1870 3989 1870
Size: 2119 4545
XmnI GAAnn'nnTTC
Cuts at: 4793 4793
Size: 6664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SfoI SrfI
SwaI