Restriction map for
pFN21AB7891
(Circular) (Six-base) MAPSORT of: pFN21AB7891.seq Check: 2660 from: 1 to: 6469
pFN21AB7891 6469 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3812 1937
Size: 1875 4594
Acc65I G'GTAC_C
Cuts at: 2367 3790 2367
Size: 1423 5046
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3134 4532 5916 6184 1632
Size: 1502 1398 1384 268 1917
Fragments arranged by size:
1917 1502 1398 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 2506 5198 2506
Size: 2692 3777
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2465 1184
Size: 1281 5188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2324 2356 2324
Size: 32 6437
AlwNI CAG_nnn'CTG
Cuts at: 3379 4212 5784 3379
Size: 833 1572 4064
Fragments arranged by size:
4064 1572 833
ApaI G_GGCC'C
Cuts at: 3544 3544
Size: 6469
ApaLI G'TGCA_C
Cuts at: 3106 4526 5682 3106
Size: 1420 1156 3893
Fragments arranged by size:
3893 1420 1156
ApoI r'AATT_y
Cuts at: 1493 3778 4004 4156 6265 1493
Size: 2285 226 152 2109 1697
Fragments arranged by size:
2285 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 3168 3249 3386 3537 3794 1870
Size: 73 156 1069 81 137 151 257 4545
Fragments arranged by size:
4545 1069 257 156 151 137 81 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2342 2375 2342
Size: 33 6436
BamHI G'GATC_C
Cuts at: 2916 3799 4166 2916
Size: 883 367 5219
Fragments arranged by size:
5219 883 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 2367 2808 2962 3338
Size: 325 217 627 475 105 441 154 376
Cuts at: 3338 3790 5245 618
Size: 452 1455 1842
Fragments arranged by size:
1842 1455 627 475 452 441 376 325
217 154 105
BanII G_rGCy'C
Cuts at: 1391 2981 3544 3788 1391
Size: 1590 563 244 4072
Fragments arranged by size:
4072 1590 563 244
BbeI G_GCGC'C
Cuts at: 2266 2266
Size: 6469
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4660 4694 1903
Size: 34 50 34 2639 34 3678
Fragments arranged by size:
3678 2639 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BfrBI ATG'CAT
Cuts at: 2569 2569
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 3548 3713
Size: 107 122 71 631 605 406 1470 165
Cuts at: 3713 5080 136
Size: 1367 1525
Fragments arranged by size:
1525 1470 1367 631 605 406 165 122
107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 3110 3343 3544 4530 5686 1790
Size: 1320 233 201 986 1156 2573
Fragments arranged by size:
2573 1320 1156 986 233 201
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6469
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4200 5158 442
Size: 416 53 83 399 24 2783 958 1753
Fragments arranged by size:
2783 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2343 2343
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2050 2467 2502 2842 5129 1239
Size: 811 417 35 340 2287 2579
Fragments arranged by size:
2579 2287 811 417 340 35
Bpu10I CC'TnA_GC
Cuts at: 2393 2975 3579 2393
Size: 582 604 5283
Fragments arranged by size:
5283 604 582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4594 5459 5757 4594
Size: 865 298 5306
Fragments arranged by size:
5306 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2860 5132 882
Size: 617 1361 2272 2219
Fragments arranged by size:
2272 2219 1361 617
BsaAI yAC'GTr
Cuts at: 493 1204 3040 4260 493
Size: 711 1836 1220 2702
Fragments arranged by size:
2702 1836 1220 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2263 4276 4658 5293
Size: 53 83 186 936 730 2013 382 635
Cuts at: 5293 275
Size: 1451
Fragments arranged by size:
2013 1451 936 730 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4902 5300 5574 5721 6132 4902
Size: 398 274 147 411 5239
Fragments arranged by size:
5239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2594 2624 1178
Size: 30 165 30 1191 30 5023
Fragments arranged by size:
5023 1191 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2448 2459 2862 3035 3403 3583 3586
Size: 973 289 11 403 173 368 180 3
Cuts at: 3586 1186
Size: 4069
Fragments arranged by size:
4069 973 403 368 289 180 173 11
3
BseYI C'CCAG_C
Cuts at: 2010 2969 3504 3543 3735 5672 6168 2010
Size: 959 535 39 192 1937 496 2311
Fragments arranged by size:
2311 1937 959 535 496 192 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3144 1163
Size: 573 1408 4488
Fragments arranged by size:
4488 1408 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 3919 4680 5708 1540
Size: 275 186 81 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 3110 4530 4615 5686 3110
Size: 1420 85 1071 3893
Fragments arranged by size:
3893 1420 1071 85
BsmI GAATG_Cn'
Cuts at: 3061 3980 4073 3061
Size: 919 93 5457
Fragments arranged by size:
5457 919 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2941 2941
Size: 6469
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2981 3110 3343 3544 3788 4530 4615
Size: 399 1191 129 233 201 244 742 85
Cuts at: 4615 5686 1391
Size: 1071 2174
Fragments arranged by size:
2174 1191 1071 742 399 244 233 201
129 85
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2457 3387 3824 844
Size: 1613 930 437 3489
Fragments arranged by size:
3489 1613 930 437
BsrBI CCG'CTC
Cuts at: 1394 1823 3211 4356 1394
Size: 429 1388 1145 3507
Fragments arranged by size:
3507 1388 1145 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2605 4964 5138 65
Size: 1183 1357 2359 174 1396
Fragments arranged by size:
2359 1396 1357 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 2959 5113 5300 1700
Size: 110 271 878 2154 187 2869
Fragments arranged by size:
2869 2154 878 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 2438 1925
Size: 513 5956
BssSI C'ACGA_G
Cuts at: 2503 4529 5541 2503
Size: 2026 1012 3431
Fragments arranged by size:
3431 2026 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2566 3338 794
Size: 1772 772 3925
Fragments arranged by size:
3925 1772 772
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 2508 3751 1184
Size: 1324 1243 3902
Fragments arranged by size:
3902 1324 1243
BstXI CCAn_nnnn'nTGG
Cuts at: 3097 3143 3097
Size: 46 6423
BstYI r'GATC_y
Cuts at: 1972 2916 3138 3799 4166 4551 4568 6020 6106
Size: 944 222 661 367 385 17 1452 86
Cuts at: 6106 6118 6464 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 944 661 385 367 346 222
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2328 2911 2328
Size: 583 5886
BtgI C'CryG_G
Cuts at: 513 1065 2297 3418 513
Size: 552 1232 1121 3564
Fragments arranged by size:
3564 1232 1121 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5730
Fragments arranged by size:
5730 683 56
BtsI GCAGTG_nn'
Cuts at: 2991 3985 4779 4799 2991
Size: 994 794 20 4661
Fragments arranged by size:
4661 994 794 20
ClaI AT'CG_AT
Cuts at: 1554 2649 1554
Size: 1095 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 2347 3773 2347
Size: 1426 5043
DraIII CAC_nnn'GTG
Cuts at: 2881 2881
Size: 6469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 3441 3916
Size: 54 1425 50 275 18 249 1362 475
Cuts at: 3916 4805 6434 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1362 889 475 275 249 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 3916 1537
Size: 275 267 1837 4090
Fragments arranged by size:
4090 1837 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 3269 3275 4399 1353
Size: 1916 6 1124 3423
Fragments arranged by size:
3423 1916 1124 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2100 2270 5056 5430 5576 387
Size: 1064 138 511 170 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 511 374 170 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2467 2502 2842 3134 4532 5129
Size: 393 418 417 35 340 292 1398 597
Cuts at: 5129 5916 6184 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1398 787 597 418 417 393 340
292 268 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 6469
EcoO109I rG'GnC_Cy
Cuts at: 2185 2351 3715 2185
Size: 166 1364 4939
Fragments arranged by size:
4939 1364 166
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FspI TGC'GCA
Cuts at: 4975 4975
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 2073 2172 2266 2490 5616 1678
Size: 395 99 94 224 3126 2531
Fragments arranged by size:
3126 2531 395 224 99 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2186 2188 2218 2465
Size: 32 135 32 584 30 2 30 247
Cuts at: 2465 2492 2497 2524 2594 2626 5110 5142 5184
Size: 27 5 27 70 32 2484 32 42
Cuts at: 5184 5216 1373
Size: 32 2626
Fragments arranged by size:
2626 2484 584 247 135 70 42 32
32 32 32 32 30 30 27 27
5 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2215 3813 4059 669
Size: 988 281 277 1598 246 3079
Fragments arranged by size:
3079 1598 988 281 277 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6469
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KasI G'GCGC_C
Cuts at: 2262 2262
Size: 6469
KpnI G_GTAC'C
Cuts at: 2371 3794 2371
Size: 1423 5046
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 2436 2707 5583 5767 1305
Size: 897 234 271 2876 184 2007
Fragments arranged by size:
2876 2007 897 271 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4647
Fragments arranged by size:
4647 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4427 4786 4945 518
Size: 648 3261 359 159 2042
Fragments arranged by size:
3261 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2182 2299 2575 3295 3473 3547 3885
Size: 443 133 117 276 720 178 74 338
Cuts at: 3885 4562 5710 5955 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 720 677 443 338 276 245
178 133 117 74
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 6469
NarI GG'CG_CC
Cuts at: 2263 2263
Size: 6469
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5917
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NsiI A_TGCA'T
Cuts at: 2571 2571
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 2569 3430 3827 5372 1132
Size: 97 1340 861 397 1545 2229
Fragments arranged by size:
2229 1545 1340 861 397 97
PasI CC'CwG_GG
Cuts at: 1348 2363 1348
Size: 1015 5454
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PfoI T'CCnGG_A
Cuts at: 2127 2277 2385 2428 2716 3324 2127
Size: 150 108 43 288 608 5272
Fragments arranged by size:
5272 608 288 150 108 43
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PmlI CAC'GTG
Cuts at: 3040 3040
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2185 2351 2185
Size: 166 6303
PshAI GACnn'nnGTC
Cuts at: 1613 2422 1613
Size: 809 5660
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PspOMI G'GGCC_C
Cuts at: 3540 3540
Size: 6469
PstI C_TGCA'G
Cuts at: 830 2454 2986 3457 3589 3821 830
Size: 1624 532 471 132 232 3478
Fragments arranged by size:
3478 1624 532 471 232 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 3295 3473 3547 3295
Size: 178 74 6217
Fragments arranged by size:
6217 178 74
SacII CC_GC'GG
Cuts at: 2300 2300
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 3188 4717 6429 3188
Size: 1529 1712 3228
Fragments arranged by size:
3228 1712 1529
SexAI A'CCwGG_T
Cuts at: 3239 3239
Size: 6469
SfcI C'TryA_G
Cuts at: 826 1045 2450 2593 2982 3453 3585 3728 3817
Size: 219 1405 143 389 471 132 143 89
Cuts at: 3817 4952 5633 5824 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1405 1135 681 471 389 219 191
143 143 132 89
SfoI GGC'GCC
Cuts at: 2264 2264
Size: 6469
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3251 3539 3796 1872
Size: 1379 288 257 4545
Fragments arranged by size:
4545 1379 288 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3168 4573 5474 5736 820
Size: 197 926 1225 1405 901 262 1553
Fragments arranged by size:
1553 1405 1225 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3430 3827 1132
Size: 2298 397 3774
Fragments arranged by size:
3774 2298 397
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 2326 1517
Size: 809 5660
StyI C'CwwG_G
Cuts at: 513 1065 3090 3142 3646 3908 513
Size: 552 2025 52 504 262 3074
Fragments arranged by size:
3074 2025 552 504 262 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2645 2697 3503 4496 4513 4666 4851
Size: 1255 1065 52 806 993 17 153 185
Cuts at: 4851 5296 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1065 993 806 445 185 153
52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 3186 4715
Size: 274 80 33 51 1443 804 405 1529
Cuts at: 4715 6427 96
Size: 1712 138
Fragments arranged by size:
1712 1529 1443 804 405 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6429 6461 6429
Size: 32 6437
Tth111I GACn'n_nGTC
Cuts at: 1316 2195 3307 1316
Size: 879 1112 4478
Fragments arranged by size:
4478 1112 879
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3667 3667
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 3168 1943
Size: 1225 5244
XmaI C'CCGG_G
Cuts at: 1870 3249 3537 3794 1870
Size: 1379 288 257 4545
Fragments arranged by size:
4545 1379 288 257
XmnI GAAnn'nnTTC
Cuts at: 3059 4598 3059
Size: 1539 4930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbvCI BsiWI BstZ17I EcoICRI
EcoRV FalI FseI FspAI NruI PacI PspXI PsrI
RsrII SacI SanDI SapI SfiI SrfI SwaI