Restriction map for
pFN21AB7890
(Circular) (Six-base) MAPSORT of: pFN21AB7890.seq Check: 1821 from: 1 to: 6661
pFN21AB7890 6661 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4471 278
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4004 1937
Size: 2067 4594
Acc65I G'GTAC_C
Cuts at: 3982 3982
Size: 6661
AclI AA'CG_TT
Cuts at: 1563 3694 4788 5161 1563
Size: 2131 1094 373 3063
Fragments arranged by size:
3063 2131 1094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2744 3122 3477 3636 3933 4724 6108 6376
Size: 1112 378 355 159 297 791 1384 268
Cuts at: 6376 1632
Size: 1917
Fragments arranged by size:
1917 1384 1112 791 378 355 297 268
159
AfeI AGC'GCT
Cuts at: 3567 3567
Size: 6661
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6464
AflIII A'CryG_T
Cuts at: 1203 5560 6537 1203
Size: 4357 977 1327
Fragments arranged by size:
4357 1327 977
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 2575 5390 2575
Size: 2815 3846
AleI CACnn'nnGTG
Cuts at: 1166 3197 1166
Size: 2031 4630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6661
AlwNI CAG_nnn'CTG
Cuts at: 2722 4404 5976 2722
Size: 1682 1572 3407
Fragments arranged by size:
3407 1682 1572
ApaI G_GGCC'C
Cuts at: 3222 3222
Size: 6661
ApaLI G'TGCA_C
Cuts at: 4718 5874 4718
Size: 1156 5505
ApoI r'AATT_y
Cuts at: 1493 2694 2712 3020 3583 3970 4196 4348 6457
Size: 1201 18 308 563 387 226 152 2109
Cuts at: 6457 1493
Size: 1697
Fragments arranged by size:
2109 1697 1201 563 387 308 226 152
18
AseI AT'TA_AT
Cuts at: 160 1032 5215 160
Size: 872 4183 1606
Fragments arranged by size:
4183 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 3986 1870
Size: 73 185 1858 4545
Fragments arranged by size:
4545 1858 185 73
BamHI G'GATC_C
Cuts at: 3076 3991 4358 3076
Size: 915 367 5379
Fragments arranged by size:
5379 915 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3982 5437 618
Size: 325 217 627 2195 1455 1842
Fragments arranged by size:
2195 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3222 3980 1391
Size: 1831 758 4072
Fragments arranged by size:
4072 1831 758
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 3139 3315 3912 928
Size: 971 261 979 176 597 3677
Fragments arranged by size:
3677 979 971 597 261 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2744 2778 4852 4886 1903
Size: 34 807 34 2074 34 3678
Fragments arranged by size:
3678 2074 807 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4553 5763 4553
Size: 1210 5451
BclI T'GATC_A
Cuts at: 1548 2514 1548
Size: 966 5695
BfrBI ATG'CAT
Cuts at: 3757 3757
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3529 5272 136
Size: 107 122 71 631 605 1857 1743 1525
Fragments arranged by size:
1857 1743 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2314 6656 2314
Size: 4342 2319
BlpI GC'TnA_GC
Cuts at: 4078 4078
Size: 6661
Bme1580I G_kGCm'C
Cuts at: 1790 2136 3222 4722 5878 1790
Size: 346 1086 1500 1156 2573
Fragments arranged by size:
2573 1500 1156 1086 346
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6661
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3125 4392 5350
Size: 416 53 83 399 24 1708 1267 958
Cuts at: 5350 442
Size: 1753
Fragments arranged by size:
1753 1708 1267 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2303 2862 3000 3165 3180 3526 3659
Size: 886 178 559 138 165 15 346 133
Cuts at: 3659 3795 5321 1239
Size: 136 1526 2579
Fragments arranged by size:
2579 1526 886 559 346 178 165 138
136 133 15
Bpu10I CC'TnA_GC
Cuts at: 3665 3665
Size: 6661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3534 4786 5651 5949 3534
Size: 1252 865 298 4246
Fragments arranged by size:
4246 1252 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2280 2639 3644 5324 882
Size: 617 781 359 1005 1680 2219
Fragments arranged by size:
2219 1680 1005 781 617 359
BsaAI yAC'GTr
Cuts at: 493 1204 3357 3831 4452 493
Size: 711 2153 474 621 2702
Fragments arranged by size:
2702 2153 711 621 474
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4468 4850 5485 275
Size: 53 83 186 936 2935 382 635 1451
Fragments arranged by size:
2935 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5094 5492 5766 5913 6324 5094
Size: 398 274 147 411 5431
Fragments arranged by size:
5431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6436
Fragments arranged by size:
6436 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5781
BseYI C'CCAG_C
Cuts at: 2873 5864 6360 2873
Size: 2991 496 3174
Fragments arranged by size:
3174 2991 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6088
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4111 4872 5900 1540
Size: 275 186 2110 761 1028 2301
Fragments arranged by size:
2301 2110 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3704 4722 4807 5878 3704
Size: 1018 85 1071 4487
Fragments arranged by size:
4487 1071 1018 85
BsmI GAATG_Cn'
Cuts at: 3418 4172 4265 3418
Size: 754 93 5814
Fragments arranged by size:
5814 754 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 3222 3704 3980 4722 4807 5878
Size: 399 346 1086 482 276 742 85 1071
Cuts at: 5878 1391
Size: 2174
Fragments arranged by size:
2174 1086 1071 742 482 399 346 276
85
BspEI T'CCGG_A
Cuts at: 6324 6324
Size: 6661
BspHI T'CATG_A
Cuts at: 2167 4550 6280 2167
Size: 2383 1730 2548
Fragments arranged by size:
2548 2383 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4016 844
Size: 3172 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4548 1394
Size: 429 2725 3507
Fragments arranged by size:
3507 2725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3449 3855 5156 5330 65
Size: 1183 2201 406 1301 174 1396
Fragments arranged by size:
2201 1396 1301 1183 406 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5305 5492 1700
Size: 110 3495 187 2869
Fragments arranged by size:
3495 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6661
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6661
BssSI C'ACGA_G
Cuts at: 4721 5733 4721
Size: 1012 5649
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6661
BstBI TT'CG_AA
Cuts at: 2151 3841 5465 2151
Size: 1690 1624 3347
Fragments arranged by size:
3347 1690 1624
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6661
BstYI r'GATC_y
Cuts at: 1972 2314 2455 3076 3991 4358 4743 4760 6212
Size: 342 141 621 915 367 385 17 1452
Cuts at: 6212 6298 6310 6656 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 915 621 385 367 346 342
141 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 513
Size: 552 939 114 5056
Fragments arranged by size:
5056 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5922
Fragments arranged by size:
5922 683 56
BtsI GCAGTG_nn'
Cuts at: 4177 4971 4991 4177
Size: 794 20 5847
Fragments arranged by size:
5847 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6626
DraI TTT'AAA
Cuts at: 2923 3965 2923
Size: 1042 5619
DraIII CAC_nnn'GTG
Cuts at: 2798 2798
Size: 6661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5668 809
Size: 507 4352 1802
Fragments arranged by size:
4352 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3351 4108 4997
Size: 54 1425 50 275 18 1521 757 889
Cuts at: 4997 6626 8
Size: 1629 43
Fragments arranged by size:
1629 1521 1425 889 757 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2248 4591 1353
Size: 800 95 2343 3423
Fragments arranged by size:
3423 2343 800 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5248 5622 5768 387
Size: 1064 138 3659 374 146 1280
Fragments arranged by size:
3659 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2303 2744 2862 3000 3122 3165
Size: 393 493 178 441 118 138 122 43
Cuts at: 3165 3180 3477 3526 3636 3659 3795 3933 4724
Size: 15 297 49 110 23 136 138 791
Cuts at: 4724 5321 6108 6376 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 791 787 597 493 441 393 297
268 178 138 138 136 122 118 110
49 43 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 6661
EcoO109I rG'GnC_Cy
Cuts at: 3219 3426 3737 3219
Size: 207 311 6143
Fragments arranged by size:
6143 311 207
EcoRI G'AATT_C
Cuts at: 2712 3970 4348 2712
Size: 1258 378 5025
Fragments arranged by size:
5025 1258 378
EcoRV GAT'ATC
Cuts at: 3820 3820
Size: 6661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2617 2924 2617
Size: 307 6354
FspI TGC'GCA
Cuts at: 5167 5167
Size: 6661
HaeII r_GCGC'y
Cuts at: 1678 3569 5808 1678
Size: 1891 2239 2531
Fragments arranged by size:
2531 2239 1891
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 2561 2593 3766
Size: 32 135 32 868 32 89 32 1173
Cuts at: 3766 3798 5302 5334 5376 5408 1373
Size: 32 1504 32 42 32 2626
Fragments arranged by size:
2626 1504 1173 868 135 89 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4005 4251 669
Size: 988 281 2067 246 3079
Fragments arranged by size:
3079 2067 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2418 748
Size: 1670 4991
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KpnI G_GTAC'C
Cuts at: 3986 3986
Size: 6661
MfeI C'AATT_G
Cuts at: 2441 4260 2441
Size: 1819 4842
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2013 5775 5959 1305
Size: 708 3762 184 2007
Fragments arranged by size:
3762 2007 708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3353 10
Size: 54 1425 343 1521 3318
Fragments arranged by size:
3318 1521 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3197 4619 4978 5137 518
Size: 648 2031 1422 359 159 2042
Fragments arranged by size:
2042 2031 1422 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4077 4754 5902 6147 1606
Size: 2471 677 1148 245 2120
Fragments arranged by size:
2471 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5170
Fragments arranged by size:
5170 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6661
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NsiI A_TGCA'T
Cuts at: 3759 3759
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 4019 5564 1132
Size: 97 2790 1545 2229
Fragments arranged by size:
2790 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6661
PciI A'CATG_T
Cuts at: 5560 5560
Size: 6661
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3073 1108
Size: 1965 4696
PfoI T'CCnGG_A
Cuts at: 3422 3675 3422
Size: 253 6408
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PmlI CAC'GTG
Cuts at: 3357 3357
Size: 6661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4730 4762 6243 6275 4730
Size: 32 1481 32 5116
Fragments arranged by size:
5116 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3426 3737 3426
Size: 311 6350
PshAI GACnn'nnGTC
Cuts at: 1613 3139 1613
Size: 1526 5135
PsiI TTA'TAA
Cuts at: 2658 4231 2658
Size: 1573 5088
PspOMI G'GGCC_C
Cuts at: 3218 3218
Size: 6661
PstI C_TGCA'G
Cuts at: 830 4013 830
Size: 3183 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6661
SalI G'TCGA_C
Cuts at: 1936 4003 1936
Size: 2067 4594
SanDI GG'GwC_CC
Cuts at: 3737 3737
Size: 6661
SbfI CC_TGCA'GG
Cuts at: 4013 4013
Size: 6661
ScaI AGT'ACT
Cuts at: 3547 4909 6621 3547
Size: 1362 1712 3587
Fragments arranged by size:
3587 1712 1362
SexAI A'CCwGG_T
Cuts at: 3949 3949
Size: 6661
SfcI C'TryA_G
Cuts at: 826 1045 3223 4009 5144 5825 6016 826
Size: 219 2178 786 1135 681 191 1471
Fragments arranged by size:
2178 1471 1135 786 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6661
SmaI CCC'GGG
Cuts at: 1872 3988 1872
Size: 2116 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3549 4765 5666 5928 820
Size: 197 926 1606 1216 901 262 1553
Fragments arranged by size:
1606 1553 1216 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4452 493
Size: 3959 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 4019 1132
Size: 2887 3774
SspI AAT'ATT
Cuts at: 5 52 3630 4585 5
Size: 47 3578 955 2081
Fragments arranged by size:
3578 2081 955 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6661
StyI C'CwwG_G
Cuts at: 513 1065 2004 4100 513
Size: 552 939 2096 3074
Fragments arranged by size:
3074 2096 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2541 4688 4705 4858 5043 5488 325
Size: 1255 961 2147 17 153 185 445 1498
Fragments arranged by size:
2147 1498 1255 961 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2615 3545 4907
Size: 274 80 33 51 1443 638 930 1362
Cuts at: 4907 6619 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1362 930 638 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6621 6653 6621
Size: 32 6629
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6661
XbaI T'CTAG_A
Cuts at: 3997 3997
Size: 6661
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6661
XmaI C'CCGG_G
Cuts at: 1870 3986 1870
Size: 2116 4545
XmnI GAAnn'nnTTC
Cuts at: 2712 4790 2712
Size: 2078 4583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4469 276
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI
PvuII RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI XcmI