Restriction map for
pFN21AB7886
(Circular) (Six-base) MAPSORT of: pFN21AB7886.seq Check: 5723 from: 1 to: 6658
pFN21AB7886 6658 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4468 278
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4001 1937
Size: 2064 4594
Acc65I G'GTAC_C
Cuts at: 3979 3979
Size: 6658
AclI AA'CG_TT
Cuts at: 1563 4785 5158 1563
Size: 3222 373 3063
Fragments arranged by size:
3222 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 2823 3446 3530 3725 3783 4721 6105
Size: 575 616 623 84 195 58 938 1384
Cuts at: 6105 6373 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 938 623 616 575 268 195
84 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6461
AflIII A'CryG_T
Cuts at: 1203 2027 2558 5557 6534 1203
Size: 824 531 2999 977 1327
Fragments arranged by size:
2999 1327 977 824 531
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 5387 5387
Size: 6658
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6658
AlwNI CAG_nnn'CTG
Cuts at: 2519 3469 3613 4401 5973 2519
Size: 950 144 788 1572 3204
Fragments arranged by size:
3204 1572 950 788 144
ApaLI G'TGCA_C
Cuts at: 4715 5871 4715
Size: 1156 5502
ApoI r'AATT_y
Cuts at: 1493 2285 2548 2636 2730 3248 3472 3719 3967
Size: 792 263 88 94 518 224 247 248
Cuts at: 3967 4193 4345 6454 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 792 518 263 248 247 226
224 152 94 88
AseI AT'TA_AT
Cuts at: 160 1032 5212 160
Size: 872 4180 1606
Fragments arranged by size:
4180 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 2478 3983 1870
Size: 73 535 1505 4545
Fragments arranged by size:
4545 1505 535 73
BamHI G'GATC_C
Cuts at: 3988 4355 3988
Size: 367 6291
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 3979 5434 618
Size: 325 217 627 224 1968 1455 1842
Fragments arranged by size:
1968 1842 1455 627 325 224 217
BanII G_rGCy'C
Cuts at: 1391 3923 3977 1391
Size: 2532 54 4072
Fragments arranged by size:
4072 2532 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3565 3756 928
Size: 971 1666 191 3830
Fragments arranged by size:
3830 1666 971 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4849 4883 1903
Size: 34 2912 34 3678
Fragments arranged by size:
3678 2912 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4550 5760 4550
Size: 1210 5448
BclI T'GATC_A
Cuts at: 1548 2484 1548
Size: 936 5722
BfrBI ATG'CAT
Cuts at: 2777 2777
Size: 6658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5269 136
Size: 107 122 71 631 605 337 3260 1525
Fragments arranged by size:
3260 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3283 3654 6653 3283
Size: 371 2999 3288
Fragments arranged by size:
3288 2999 371
BlpI GC'TnA_GC
Cuts at: 4075 4075
Size: 6658
Bme1580I G_kGCm'C
Cuts at: 1790 3846 4719 5875 1790
Size: 2056 873 1156 2573
Fragments arranged by size:
2573 2056 1156 873
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6658
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 3942 4389
Size: 416 53 83 399 24 683 1842 447
Cuts at: 4389 5347 442
Size: 958 1753
Fragments arranged by size:
1842 1753 958 683 447 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2756 3657 5318 1239
Size: 1517 901 1661 2579
Fragments arranged by size:
2579 1661 1517 901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2740 2744 4783 5648 5946 2740
Size: 4 2039 865 298 3452
Fragments arranged by size:
3452 2039 865 298 4
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5321 882
Size: 617 3822 2219
Fragments arranged by size:
3822 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4449 493
Size: 711 3245 2702
Fragments arranged by size:
3245 2702 711
BsaBI GATnn'nnATC
Cuts at: 2535 3033 3050 3866 6652 2535
Size: 498 17 816 2786 2541
Fragments arranged by size:
2786 2541 816 498 17
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4465 4847 5482 275
Size: 53 83 186 936 2932 382 635 1451
Fragments arranged by size:
2932 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5091 5489 5763 5910 6321 5091
Size: 398 274 147 411 5428
Fragments arranged by size:
5428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3633 3663 1178
Size: 30 165 30 2230 30 4173
Fragments arranged by size:
4173 2230 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3654 1186
Size: 2468 4190
BseYI C'CCAG_C
Cuts at: 2074 5861 6357 2074
Size: 3787 496 2375
Fragments arranged by size:
3787 2375 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3287 3643 3671 1163
Size: 573 1551 356 28 4150
Fragments arranged by size:
4150 1551 573 356 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 4108 4869 5897 1540
Size: 275 186 48 2059 761 1028 2301
Fragments arranged by size:
2301 2059 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 4719 4804 5875 4719
Size: 85 1071 5502
Fragments arranged by size:
5502 1071 85
BsmI GAATG_Cn'
Cuts at: 2779 3341 4169 4262 2779
Size: 562 828 93 5175
Fragments arranged by size:
5175 828 562 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3846 3923 3977 4719 4804 5875 1391
Size: 399 2056 77 54 742 85 1071 2174
Fragments arranged by size:
2174 2056 1071 742 399 85 77 54
BspEI T'CCGG_A
Cuts at: 6321 6321
Size: 6658
BspHI T'CATG_A
Cuts at: 4547 6277 4547
Size: 1730 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4013 844
Size: 3169 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 3481 4545 1394
Size: 429 1609 49 1064 3507
Fragments arranged by size:
3507 1609 1064 429 49
BsrDI GCAATG_nn'
Cuts at: 65 1248 5153 5327 65
Size: 1183 3905 174 1396
Fragments arranged by size:
3905 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5302 5489 1700
Size: 110 3492 187 2869
Fragments arranged by size:
3492 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3094 3936 96
Size: 2998 842 2818
Fragments arranged by size:
2998 2818 842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6658
BssSI C'ACGA_G
Cuts at: 4718 5730 4718
Size: 1012 5646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6658
BstBI TT'CG_AA
Cuts at: 2650 5462 2650
Size: 2812 3846
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6658
BstYI r'GATC_y
Cuts at: 1972 2242 3028 3283 3654 3988 4355 4740 4757
Size: 270 786 255 371 334 367 385 17
Cuts at: 4757 6209 6295 6307 6653 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 786 385 371 367 346 334
270 255 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2606 2841 3361 513
Size: 552 939 602 235 520 3810
Fragments arranged by size:
3810 939 602 552 520 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2514 3363 487
Size: 683 56 1288 849 3782
Fragments arranged by size:
3782 1288 849 683 56
BtsI GCAGTG_nn'
Cuts at: 4174 4968 4988 4174
Size: 794 20 5844
Fragments arranged by size:
5844 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2610 2645 3897 3915 3932 3950 529
Size: 35 2046 35 1252 18 17 18 3237
Fragments arranged by size:
3237 2046 1252 35 35 18 18 17
DraI TTT'AAA
Cuts at: 3962 3962
Size: 6658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5665 809
Size: 507 4349 1802
Fragments arranged by size:
4349 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 4105 4994
Size: 54 1425 50 275 18 216 2059 889
Cuts at: 4994 6623 8
Size: 1629 43
Fragments arranged by size:
2059 1629 1425 889 275 216 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 4105 1537
Size: 275 234 2059 4090
Fragments arranged by size:
4090 2059 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 3701 3759 4588 1353
Size: 1026 1322 58 829 3423
Fragments arranged by size:
3423 1322 1026 829 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5245 5619 5765 387
Size: 1064 138 3656 374 146 1280
Fragments arranged by size:
3656 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2756 2823 3446 3530 3657 3725
Size: 393 575 549 67 623 84 127 68
Cuts at: 3725 3783 4721 5318 6105 6373 1239
Size: 58 938 597 787 268 1524
Fragments arranged by size:
1524 938 787 623 597 575 549 393
268 127 84 68 67 58
EcoO109I rG'GnC_Cy
Cuts at: 3178 3178
Size: 6658
EcoRI G'AATT_C
Cuts at: 2285 3967 4345 2285
Size: 1682 378 4598
Fragments arranged by size:
4598 1682 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2370 2370
Size: 6658
FspI TGC'GCA
Cuts at: 5164 5164
Size: 6658
HaeII r_GCGC'y
Cuts at: 1678 2944 5805 1678
Size: 1266 2861 2531
Fragments arranged by size:
2861 2531 1266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2430 2462 2911 2943 3586
Size: 32 135 32 858 32 449 32 643
Cuts at: 3586 3618 5299 5331 5373 5405 1373
Size: 32 1681 32 42 32 2626
Fragments arranged by size:
2626 1681 858 643 449 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3295 3535 4002 4248 669
Size: 988 281 1357 240 467 246 3079
Fragments arranged by size:
3079 1357 988 467 281 246 240
HindIII A'AGCT_T
Cuts at: 748 3229 748
Size: 2481 4177
HpaI GTT'AAC
Cuts at: 3295 3535 4248 3295
Size: 240 713 5705
Fragments arranged by size:
5705 713 240
KpnI G_GTAC'C
Cuts at: 3983 3983
Size: 6658
MfeI C'AATT_G
Cuts at: 4257 4257
Size: 6658
MluI A'CGCG_T
Cuts at: 2027 6534 2027
Size: 4507 2151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2738 2745 5772 5956 1305
Size: 1433 7 3027 184 2007
Fragments arranged by size:
3027 2007 1433 184 7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4836
Fragments arranged by size:
4836 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2671 2954 4616 4975 5134 518
Size: 648 1505 283 1662 359 159 2042
Fragments arranged by size:
2042 1662 1505 648 359 283 159
MspA1I CmG'CkG
Cuts at: 1606 2236 3635 3670 4074 4751 5899 6144 1606
Size: 630 1399 35 404 677 1148 245 2120
Fragments arranged by size:
2120 1399 1148 677 630 404 245 35
NcoI C'CATG_G
Cuts at: 513 1065 2004 3361 513
Size: 552 939 1357 3810
Fragments arranged by size:
3810 1357 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6658
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 2046 4105 2046
Size: 2059 4599
NruI TCG'CGA
Cuts at: 3039 3039
Size: 6658
NsiI A_TGCA'T
Cuts at: 2779 2779
Size: 6658
NspI r_CATG'y
Cuts at: 1132 1229 2562 3943 4016 5561 1132
Size: 97 1333 1381 73 1545 2229
Fragments arranged by size:
2229 1545 1381 1333 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6658
PciI A'CATG_T
Cuts at: 2558 5557 2558
Size: 2999 3659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6658
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4759 6240 6272 4727
Size: 32 1481 32 5113
Fragments arranged by size:
5113 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3178 3178
Size: 6658
PshAI GACnn'nnGTC
Cuts at: 1613 2177 2559 1613
Size: 564 382 5712
Fragments arranged by size:
5712 564 382
PsiI TTA'TAA
Cuts at: 4228 4228
Size: 6658
PstI C_TGCA'G
Cuts at: 830 2065 4010 830
Size: 1235 1945 3478
Fragments arranged by size:
3478 1945 1235
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
PvuII CAG'CTG
Cuts at: 2236 2236
Size: 6658
SalI G'TCGA_C
Cuts at: 1936 4000 1936
Size: 2064 4594
SapI GCTCTTCn'nnn_
Cuts at: 3759 3759
Size: 6658
SbfI CC_TGCA'GG
Cuts at: 4010 4010
Size: 6658
ScaI AGT'ACT
Cuts at: 4906 6618 4906
Size: 1712 4946
SfcI C'TryA_G
Cuts at: 826 1045 2061 2931 3118 3770 4006 5141 5822
Size: 219 1016 870 187 652 236 1135 681
Cuts at: 5822 6013 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1016 870 681 652 236 219
191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 3985 1872
Size: 2113 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2719 2759 4762 5663 5925 820
Size: 197 926 776 40 2003 901 262 1553
Fragments arranged by size:
2003 1553 926 901 776 262 197 40
SnaBI TAC'GTA
Cuts at: 493 4449 493
Size: 3956 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6658
SphI G_CATG'C
Cuts at: 1132 4016 1132
Size: 2884 3774
SspI AAT'ATT
Cuts at: 5 52 4582 5
Size: 47 4530 2081
Fragments arranged by size:
4530 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6658
StyI C'CwwG_G
Cuts at: 513 1065 2004 2354 3361 4097 513
Size: 552 939 350 1007 736 3074
Fragments arranged by size:
3074 1007 939 736 552 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3092 4685 4702 4855 5040 5485 325
Size: 1255 1512 1593 17 153 185 445 1498
Fragments arranged by size:
1593 1512 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 2304 3094
Size: 274 80 33 51 1443 213 114 790
Cuts at: 3094 3936 4904 6616 96
Size: 842 968 1712 138
Fragments arranged by size:
1712 1443 968 842 790 274 213 138
114 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3633 3665 6618 6650 3633
Size: 32 2953 32 3641
Fragments arranged by size:
3641 2953 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2442 1316
Size: 1126 5532
XbaI T'CTAG_A
Cuts at: 3994 3994
Size: 6658
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2604 2604
Size: 6658
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6658
XmaI C'CCGG_G
Cuts at: 1870 3983 1870
Size: 2113 4545
XmnI GAAnn'nnTTC
Cuts at: 2637 3904 4787 2637
Size: 1267 883 4508
Fragments arranged by size:
4508 1267 883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4466 276
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI