(Circular) (Six-base) MAPSORT of: pFN21AB7881.seq Check: 8299 from: 1 to: 6439 pFN21AB7881 6439 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4249 278 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2570 3782 1937 Size: 633 1212 4594 Fragments arranged by size: 4594 1212 633 Acc65I G'GTAC_C Cuts at: 3760 3760 Size: 6439 AclI AA'CG_TT Cuts at: 1563 4566 4939 1563 Size: 3003 373 3063 Fragments arranged by size: 3063 3003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2013 2031 2079 2149 3064 3148 3400 3484 Size: 381 18 48 70 915 84 252 84 Cuts at: 3484 4502 5886 6154 1632 Size: 1018 1384 268 1917 Fragments arranged by size: 1917 1384 1018 915 381 268 252 84 84 70 48 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6242 AflIII A'CryG_T Cuts at: 1203 2766 5338 6315 1203 Size: 1563 2572 977 1327 Fragments arranged by size: 2572 1563 1327 977 AgeI A'CCGG_T Cuts at: 5270 5270 Size: 6439 AhdI GACnn_n'nnGTC Cuts at: 2420 5168 2420 Size: 2748 3691 AleI CACnn'nnGTG Cuts at: 1166 3711 1166 Size: 2545 3894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6439 AlwNI CAG_nnn'CTG Cuts at: 2242 2275 2332 2480 4182 5754 2242 Size: 33 57 148 1702 1572 2927 Fragments arranged by size: 2927 1702 1572 148 57 33 ApaI G_GGCC'C Cuts at: 2227 2227 Size: 6439 ApaLI G'TGCA_C Cuts at: 4496 5652 4496 Size: 1156 5283 ApoI r'AATT_y Cuts at: 1493 2755 3618 3748 3974 4126 6235 1493 Size: 1262 863 130 226 152 2109 1697 Fragments arranged by size: 2109 1697 1262 863 226 152 130 AseI AT'TA_AT Cuts at: 160 1032 4993 160 Size: 872 3961 1606 Fragments arranged by size: 3961 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 AvaI C'yCGr_G Cuts at: 1870 1943 2199 2910 3764 1870 Size: 73 256 711 854 4545 Fragments arranged by size: 4545 854 711 256 73 BamHI G'GATC_C Cuts at: 3769 4136 3769 Size: 367 6072 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3760 5215 618 Size: 325 217 627 1973 1455 1842 Fragments arranged by size: 1973 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2227 2325 3008 3428 3758 1391 Size: 836 98 683 420 330 4072 Fragments arranged by size: 4072 836 683 420 330 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2679 928 Size: 971 780 4688 Fragments arranged by size: 4688 971 780 BbvCI CC'TCA_GC Cuts at: 2243 2243 Size: 6439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4630 4664 1903 Size: 34 2693 34 3678 Fragments arranged by size: 3678 2693 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4331 5541 4331 Size: 1210 5229 BclI T'GATC_A Cuts at: 1548 1548 Size: 6439 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5050 136 Size: 107 122 71 631 605 3378 1525 Fragments arranged by size: 3378 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6434 6434 Size: 6439 BlpI GC'TnA_GC Cuts at: 3856 3856 Size: 6439 Bme1580I G_kGCm'C Cuts at: 1790 2227 2544 4500 5656 1790 Size: 437 317 1956 1156 2573 Fragments arranged by size: 2573 1956 1156 437 317 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6439 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3048 3216 3384 Size: 416 53 83 399 24 1631 168 168 Cuts at: 3384 3468 4170 5128 442 Size: 84 702 958 1753 Fragments arranged by size: 1753 1631 958 702 416 399 168 168 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6439 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2604 2604 Size: 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2309 2312 2520 2631 3046 3564 5099 Size: 1058 12 3 208 111 415 518 1535 Cuts at: 5099 1239 Size: 2579 Fragments arranged by size: 2579 1535 1058 518 415 208 111 12 3 Bpu10I CC'TnA_GC Cuts at: 2243 3007 2243 Size: 764 5675 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 4564 5429 5727 2915 Size: 1649 865 298 3627 Fragments arranged by size: 3627 1649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2607 2780 2907 3292 3605 5102 882 Size: 617 1108 173 127 385 313 1497 2219 Fragments arranged by size: 2219 1497 1108 617 385 313 173 127 BsaAI yAC'GTr Cuts at: 493 1204 4230 493 Size: 711 3026 2702 Fragments arranged by size: 3026 2702 711 BsaBI GATnn'nnATC Cuts at: 6433 6433 Size: 6439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4246 4628 5263 275 Size: 53 83 186 936 2713 382 635 1451 Fragments arranged by size: 2713 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4872 5270 5544 5691 6102 4872 Size: 398 274 147 411 5209 Fragments arranged by size: 5209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2352 2382 2756 2786 1178 Size: 30 165 30 949 30 374 30 4831 Fragments arranged by size: 4831 949 374 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2333 2378 2474 2714 3463 1186 Size: 916 231 45 96 240 749 4162 Fragments arranged by size: 4162 916 749 240 231 96 45 BseYI C'CCAG_C Cuts at: 5642 6138 5642 Size: 496 5943 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2466 3173 3257 1163 Size: 573 730 707 84 4345 Fragments arranged by size: 4345 730 707 573 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3889 4650 5678 1540 Size: 275 186 1888 761 1028 2301 Fragments arranged by size: 2301 1888 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 3008 3428 4500 4585 5656 2325 Size: 683 420 1072 85 1071 3108 Fragments arranged by size: 3108 1072 1071 683 420 85 BsmI GAATG_Cn' Cuts at: 3950 4043 3950 Size: 93 6346 BsmBI CGTCTCn'nnnn_ Cuts at: 2956 2956 Size: 6439 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2227 2325 2544 3008 3428 3758 4500 Size: 399 437 98 219 464 420 330 742 Cuts at: 4500 4585 5656 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 464 437 420 399 330 219 98 85 BspEI T'CCGG_A Cuts at: 6102 6102 Size: 6439 BspHI T'CATG_A Cuts at: 4328 6058 4328 Size: 1730 4709 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3794 844 Size: 2950 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4326 1394 Size: 429 2503 3507 Fragments arranged by size: 3507 2503 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3487 4934 5108 65 Size: 1183 2239 1447 174 1396 Fragments arranged by size: 2239 1447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5083 5270 1700 Size: 110 3273 187 2869 Fragments arranged by size: 3273 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6439 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6439 BssSI C'ACGA_G Cuts at: 4499 5511 4499 Size: 1012 5427 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6439 BstBI TT'CG_AA Cuts at: 5243 5243 Size: 6439 BstEII G'GTnAC_C Cuts at: 1184 2371 1184 Size: 1187 5252 BstXI CCAn_nnnn'nTGG Cuts at: 2849 2849 Size: 6439 BstYI r'GATC_y Cuts at: 1972 2065 2271 2728 3769 4136 4521 4538 5990 Size: 93 206 457 1041 367 385 17 1452 Cuts at: 5990 6076 6088 6434 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1041 457 385 367 346 206 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2316 2316 Size: 6439 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4948 Fragments arranged by size: 4948 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5700 Fragments arranged by size: 5700 683 56 BtsI GCAGTG_nn' Cuts at: 3955 4749 4769 3955 Size: 794 20 5625 Fragments arranged by size: 5625 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3217 3252 3469 3504 529 Size: 35 2653 35 217 35 3464 Fragments arranged by size: 3464 2653 217 35 35 35 DraI TTT'AAA Cuts at: 3743 3743 Size: 6439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5446 809 Size: 507 4130 1802 Fragments arranged by size: 4130 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2140 3886 4775 Size: 54 1425 50 275 18 310 1746 889 Cuts at: 4775 6404 8 Size: 1629 43 Fragments arranged by size: 1746 1629 1425 889 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3886 1537 Size: 275 2074 4090 Fragments arranged by size: 4090 2074 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 4369 1353 Size: 702 2314 3423 Fragments arranged by size: 3423 2314 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2144 3436 5026 5400 5546 387 Size: 1064 138 555 1292 1590 374 146 1280 Fragments arranged by size: 1590 1292 1280 1064 555 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 3006 3426 2323 Size: 683 420 5336 Fragments arranged by size: 5336 683 420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2031 2079 2149 2297 2309 2312 Size: 393 381 18 48 70 148 12 3 Cuts at: 2312 2520 2631 3046 3064 3148 3400 3484 3564 Size: 208 111 415 18 84 252 84 80 Cuts at: 3564 4502 5099 5886 6154 1239 Size: 938 597 787 268 1524 Fragments arranged by size: 1524 938 787 597 415 393 381 268 252 208 148 111 84 84 80 70 48 18 18 12 3 EcoNI CCTnn'n_nnAGG Cuts at: 2315 2381 2315 Size: 66 6373 EcoO109I rG'GnC_Cy Cuts at: 2195 2224 3495 2195 Size: 29 1271 5139 Fragments arranged by size: 5139 1271 29 EcoRI G'AATT_C Cuts at: 3618 3748 4126 3618 Size: 130 378 5931 Fragments arranged by size: 5931 378 130 EcoRV GAT'ATC Cuts at: 3115 3115 Size: 6439 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2043 2084 2043 Size: 41 6398 FspI TGC'GCA Cuts at: 4945 4945 Size: 6439 HaeII r_GCGC'y Cuts at: 1678 5586 1678 Size: 3908 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2406 2438 3415 Size: 32 135 32 524 32 278 32 977 Cuts at: 3415 3447 3667 3699 5080 5112 5154 5186 1373 Size: 32 220 32 1381 32 42 32 2626 Fragments arranged by size: 2626 1381 977 524 278 220 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3783 4029 669 Size: 988 281 1845 246 3079 Fragments arranged by size: 3079 1845 988 281 246 HindIII A'AGCT_T Cuts at: 748 2992 3327 3579 748 Size: 2244 335 252 3608 Fragments arranged by size: 3608 2244 335 252 HpaI GTT'AAC Cuts at: 4029 4029 Size: 6439 KpnI G_GTAC'C Cuts at: 3764 3764 Size: 6439 MfeI C'AATT_G Cuts at: 4038 4038 Size: 6439 MluI A'CGCG_T Cuts at: 6315 6315 Size: 6439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2239 5553 5737 1305 Size: 934 3314 184 2007 Fragments arranged by size: 3314 2007 934 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2142 10 Size: 54 1425 343 310 4307 Fragments arranged by size: 4307 1425 343 310 54 MslI CAynn'nnrTG Cuts at: 518 1166 2681 2973 3223 3711 4397 4756 4915 Size: 648 1515 292 250 488 686 359 159 Cuts at: 4915 518 Size: 2042 Fragments arranged by size: 2042 1515 686 648 488 359 292 250 159 MspA1I CmG'CkG Cuts at: 1606 2861 2941 3855 4532 5680 5925 1606 Size: 1255 80 914 677 1148 245 2120 Fragments arranged by size: 2120 1255 1148 914 677 245 80 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4948 Fragments arranged by size: 4948 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6439 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6439 NotI GC'GGCC_GC Cuts at: 3886 3886 Size: 6439 NspI r_CATG'y Cuts at: 1132 1229 2770 3797 5342 1132 Size: 97 1541 1027 1545 2229 Fragments arranged by size: 2229 1545 1541 1027 97 PasI CC'CwG_GG Cuts at: 1348 2776 1348 Size: 1428 5011 PciI A'CATG_T Cuts at: 2766 5338 2766 Size: 2572 3867 PflMI CCAn_nnn'nTGG Cuts at: 1108 2332 2659 1108 Size: 1224 327 4888 Fragments arranged by size: 4888 1224 327 PfoI T'CCnGG_A Cuts at: 2274 2274 Size: 6439 PmeI GTTT'AAAC Cuts at: 3743 3743 Size: 6439 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4508 4540 6021 6053 4508 Size: 32 1481 32 4894 Fragments arranged by size: 4894 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2195 2195 Size: 6439 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6439 PsiI TTA'TAA Cuts at: 4009 4009 Size: 6439 PspOMI G'GGCC_C Cuts at: 2223 2223 Size: 6439 PstI C_TGCA'G Cuts at: 830 3791 830 Size: 2961 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6439 PvuII CAG'CTG Cuts at: 2861 2861 Size: 6439 SacI G_AGCT'C Cuts at: 2325 3008 3428 2325 Size: 683 420 5336 Fragments arranged by size: 5336 683 420 SalI G'TCGA_C Cuts at: 1936 3781 1936 Size: 1845 4594 SbfI CC_TGCA'GG Cuts at: 3791 3791 Size: 6439 ScaI AGT'ACT Cuts at: 4687 6399 4687 Size: 1712 4727 SfcI C'TryA_G Cuts at: 826 1045 3787 4922 5603 5794 826 Size: 219 2742 1135 681 191 1471 Fragments arranged by size: 2742 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6439 SmaI CCC'GGG Cuts at: 1872 3766 1872 Size: 1894 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2894 4543 5444 5706 820 Size: 197 926 951 1649 901 262 1553 Fragments arranged by size: 1649 1553 951 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4230 493 Size: 3737 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6439 SphI G_CATG'C Cuts at: 1132 3797 1132 Size: 2665 3774 SspI AAT'ATT Cuts at: 5 52 4363 5 Size: 47 4311 2081 Fragments arranged by size: 4311 2081 47 StuI AGG'CCT Cuts at: 1517 3012 3180 3516 1517 Size: 1495 168 336 4440 Fragments arranged by size: 4440 1495 336 168 StyI C'CwwG_G Cuts at: 513 1065 2004 2262 3878 513 Size: 552 939 258 1616 3074 Fragments arranged by size: 3074 1616 939 552 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2768 4466 4483 4636 4821 5266 325 Size: 1255 1188 1698 17 153 185 445 1498 Fragments arranged by size: 1698 1498 1255 1188 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2467 4685 6397 Size: 274 80 33 51 1443 490 2218 1712 Cuts at: 6397 96 Size: 138 Fragments arranged by size: 2218 1712 1443 490 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2352 2384 6399 6431 2352 Size: 32 4015 32 2360 Fragments arranged by size: 4015 2360 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6439 XbaI T'CTAG_A Cuts at: 3775 3775 Size: 6439 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6439 XmaI C'CCGG_G Cuts at: 1870 3764 1870 Size: 1894 4545 XmnI GAAnn'nnTTC Cuts at: 2026 3245 3329 3413 3581 4568 2026 Size: 1219 84 84 168 987 3897 Fragments arranged by size: 3897 1219 987 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4247 276 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI