Restriction map for
pFN21AB7881
(Circular) (Six-base) MAPSORT of: pFN21AB7881.seq Check: 8299 from: 1 to: 6439
pFN21AB7881 6439 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4249 278
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2570 3782 1937
Size: 633 1212 4594
Fragments arranged by size:
4594 1212 633
Acc65I G'GTAC_C
Cuts at: 3760 3760
Size: 6439
AclI AA'CG_TT
Cuts at: 1563 4566 4939 1563
Size: 3003 373 3063
Fragments arranged by size:
3063 3003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2013 2031 2079 2149 3064 3148 3400 3484
Size: 381 18 48 70 915 84 252 84
Cuts at: 3484 4502 5886 6154 1632
Size: 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1018 915 381 268 252 84
84 70 48 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6242
AflIII A'CryG_T
Cuts at: 1203 2766 5338 6315 1203
Size: 1563 2572 977 1327
Fragments arranged by size:
2572 1563 1327 977
AgeI A'CCGG_T
Cuts at: 5270 5270
Size: 6439
AhdI GACnn_n'nnGTC
Cuts at: 2420 5168 2420
Size: 2748 3691
AleI CACnn'nnGTG
Cuts at: 1166 3711 1166
Size: 2545 3894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6439
AlwNI CAG_nnn'CTG
Cuts at: 2242 2275 2332 2480 4182 5754 2242
Size: 33 57 148 1702 1572 2927
Fragments arranged by size:
2927 1702 1572 148 57 33
ApaI G_GGCC'C
Cuts at: 2227 2227
Size: 6439
ApaLI G'TGCA_C
Cuts at: 4496 5652 4496
Size: 1156 5283
ApoI r'AATT_y
Cuts at: 1493 2755 3618 3748 3974 4126 6235 1493
Size: 1262 863 130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1262 863 226 152 130
AseI AT'TA_AT
Cuts at: 160 1032 4993 160
Size: 872 3961 1606
Fragments arranged by size:
3961 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2910 3764 1870
Size: 73 256 711 854 4545
Fragments arranged by size:
4545 854 711 256 73
BamHI G'GATC_C
Cuts at: 3769 4136 3769
Size: 367 6072
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3760 5215 618
Size: 325 217 627 1973 1455 1842
Fragments arranged by size:
1973 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2227 2325 3008 3428 3758 1391
Size: 836 98 683 420 330 4072
Fragments arranged by size:
4072 836 683 420 330 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2679 928
Size: 971 780 4688
Fragments arranged by size:
4688 971 780
BbvCI CC'TCA_GC
Cuts at: 2243 2243
Size: 6439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4630 4664 1903
Size: 34 2693 34 3678
Fragments arranged by size:
3678 2693 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4331 5541 4331
Size: 1210 5229
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6439
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5050 136
Size: 107 122 71 631 605 3378 1525
Fragments arranged by size:
3378 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6434 6434
Size: 6439
BlpI GC'TnA_GC
Cuts at: 3856 3856
Size: 6439
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2544 4500 5656 1790
Size: 437 317 1956 1156 2573
Fragments arranged by size:
2573 1956 1156 437 317
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6439
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3048 3216 3384
Size: 416 53 83 399 24 1631 168 168
Cuts at: 3384 3468 4170 5128 442
Size: 84 702 958 1753
Fragments arranged by size:
1753 1631 958 702 416 399 168 168
84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2604 2604
Size: 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2309 2312 2520 2631 3046 3564 5099
Size: 1058 12 3 208 111 415 518 1535
Cuts at: 5099 1239
Size: 2579
Fragments arranged by size:
2579 1535 1058 518 415 208 111 12
3
Bpu10I CC'TnA_GC
Cuts at: 2243 3007 2243
Size: 764 5675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 4564 5429 5727 2915
Size: 1649 865 298 3627
Fragments arranged by size:
3627 1649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2607 2780 2907 3292 3605 5102 882
Size: 617 1108 173 127 385 313 1497 2219
Fragments arranged by size:
2219 1497 1108 617 385 313 173 127
BsaAI yAC'GTr
Cuts at: 493 1204 4230 493
Size: 711 3026 2702
Fragments arranged by size:
3026 2702 711
BsaBI GATnn'nnATC
Cuts at: 6433 6433
Size: 6439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4246 4628 5263 275
Size: 53 83 186 936 2713 382 635 1451
Fragments arranged by size:
2713 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4872 5270 5544 5691 6102 4872
Size: 398 274 147 411 5209
Fragments arranged by size:
5209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2352 2382 2756 2786 1178
Size: 30 165 30 949 30 374 30 4831
Fragments arranged by size:
4831 949 374 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2333 2378 2474 2714 3463 1186
Size: 916 231 45 96 240 749 4162
Fragments arranged by size:
4162 916 749 240 231 96 45
BseYI C'CCAG_C
Cuts at: 5642 6138 5642
Size: 496 5943
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2466 3173 3257 1163
Size: 573 730 707 84 4345
Fragments arranged by size:
4345 730 707 573 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3889 4650 5678 1540
Size: 275 186 1888 761 1028 2301
Fragments arranged by size:
2301 1888 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 3008 3428 4500 4585 5656 2325
Size: 683 420 1072 85 1071 3108
Fragments arranged by size:
3108 1072 1071 683 420 85
BsmI GAATG_Cn'
Cuts at: 3950 4043 3950
Size: 93 6346
BsmBI CGTCTCn'nnnn_
Cuts at: 2956 2956
Size: 6439
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2227 2325 2544 3008 3428 3758 4500
Size: 399 437 98 219 464 420 330 742
Cuts at: 4500 4585 5656 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 464 437 420 399 330
219 98 85
BspEI T'CCGG_A
Cuts at: 6102 6102
Size: 6439
BspHI T'CATG_A
Cuts at: 4328 6058 4328
Size: 1730 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3794 844
Size: 2950 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4326 1394
Size: 429 2503 3507
Fragments arranged by size:
3507 2503 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3487 4934 5108 65
Size: 1183 2239 1447 174 1396
Fragments arranged by size:
2239 1447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5083 5270 1700
Size: 110 3273 187 2869
Fragments arranged by size:
3273 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6439
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6439
BssSI C'ACGA_G
Cuts at: 4499 5511 4499
Size: 1012 5427
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6439
BstBI TT'CG_AA
Cuts at: 5243 5243
Size: 6439
BstEII G'GTnAC_C
Cuts at: 1184 2371 1184
Size: 1187 5252
BstXI CCAn_nnnn'nTGG
Cuts at: 2849 2849
Size: 6439
BstYI r'GATC_y
Cuts at: 1972 2065 2271 2728 3769 4136 4521 4538 5990
Size: 93 206 457 1041 367 385 17 1452
Cuts at: 5990 6076 6088 6434 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1041 457 385 367 346 206
93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2316 2316
Size: 6439
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4948
Fragments arranged by size:
4948 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5700
Fragments arranged by size:
5700 683 56
BtsI GCAGTG_nn'
Cuts at: 3955 4749 4769 3955
Size: 794 20 5625
Fragments arranged by size:
5625 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3217 3252 3469 3504 529
Size: 35 2653 35 217 35 3464
Fragments arranged by size:
3464 2653 217 35 35 35
DraI TTT'AAA
Cuts at: 3743 3743
Size: 6439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5446 809
Size: 507 4130 1802
Fragments arranged by size:
4130 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 3886 4775
Size: 54 1425 50 275 18 310 1746 889
Cuts at: 4775 6404 8
Size: 1629 43
Fragments arranged by size:
1746 1629 1425 889 310 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3886 1537
Size: 275 2074 4090
Fragments arranged by size:
4090 2074 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 4369 1353
Size: 702 2314 3423
Fragments arranged by size:
3423 2314 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 3436 5026 5400 5546 387
Size: 1064 138 555 1292 1590 374 146 1280
Fragments arranged by size:
1590 1292 1280 1064 555 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 3006 3426 2323
Size: 683 420 5336
Fragments arranged by size:
5336 683 420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2031 2079 2149 2297 2309 2312
Size: 393 381 18 48 70 148 12 3
Cuts at: 2312 2520 2631 3046 3064 3148 3400 3484 3564
Size: 208 111 415 18 84 252 84 80
Cuts at: 3564 4502 5099 5886 6154 1239
Size: 938 597 787 268 1524
Fragments arranged by size:
1524 938 787 597 415 393 381 268
252 208 148 111 84 84 80 70
48 18 18 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 2381 2315
Size: 66 6373
EcoO109I rG'GnC_Cy
Cuts at: 2195 2224 3495 2195
Size: 29 1271 5139
Fragments arranged by size:
5139 1271 29
EcoRI G'AATT_C
Cuts at: 3618 3748 4126 3618
Size: 130 378 5931
Fragments arranged by size:
5931 378 130
EcoRV GAT'ATC
Cuts at: 3115 3115
Size: 6439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2043 2084 2043
Size: 41 6398
FspI TGC'GCA
Cuts at: 4945 4945
Size: 6439
HaeII r_GCGC'y
Cuts at: 1678 5586 1678
Size: 3908 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2406 2438 3415
Size: 32 135 32 524 32 278 32 977
Cuts at: 3415 3447 3667 3699 5080 5112 5154 5186 1373
Size: 32 220 32 1381 32 42 32 2626
Fragments arranged by size:
2626 1381 977 524 278 220 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3783 4029 669
Size: 988 281 1845 246 3079
Fragments arranged by size:
3079 1845 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2992 3327 3579 748
Size: 2244 335 252 3608
Fragments arranged by size:
3608 2244 335 252
HpaI GTT'AAC
Cuts at: 4029 4029
Size: 6439
KpnI G_GTAC'C
Cuts at: 3764 3764
Size: 6439
MfeI C'AATT_G
Cuts at: 4038 4038
Size: 6439
MluI A'CGCG_T
Cuts at: 6315 6315
Size: 6439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2239 5553 5737 1305
Size: 934 3314 184 2007
Fragments arranged by size:
3314 2007 934 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2142 10
Size: 54 1425 343 310 4307
Fragments arranged by size:
4307 1425 343 310 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2681 2973 3223 3711 4397 4756 4915
Size: 648 1515 292 250 488 686 359 159
Cuts at: 4915 518
Size: 2042
Fragments arranged by size:
2042 1515 686 648 488 359 292 250
159
MspA1I CmG'CkG
Cuts at: 1606 2861 2941 3855 4532 5680 5925 1606
Size: 1255 80 914 677 1148 245 2120
Fragments arranged by size:
2120 1255 1148 914 677 245 80
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4948
Fragments arranged by size:
4948 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6439
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6439
NotI GC'GGCC_GC
Cuts at: 3886 3886
Size: 6439
NspI r_CATG'y
Cuts at: 1132 1229 2770 3797 5342 1132
Size: 97 1541 1027 1545 2229
Fragments arranged by size:
2229 1545 1541 1027 97
PasI CC'CwG_GG
Cuts at: 1348 2776 1348
Size: 1428 5011
PciI A'CATG_T
Cuts at: 2766 5338 2766
Size: 2572 3867
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 2659 1108
Size: 1224 327 4888
Fragments arranged by size:
4888 1224 327
PfoI T'CCnGG_A
Cuts at: 2274 2274
Size: 6439
PmeI GTTT'AAAC
Cuts at: 3743 3743
Size: 6439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4508 4540 6021 6053 4508
Size: 32 1481 32 4894
Fragments arranged by size:
4894 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2195 2195
Size: 6439
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6439
PsiI TTA'TAA
Cuts at: 4009 4009
Size: 6439
PspOMI G'GGCC_C
Cuts at: 2223 2223
Size: 6439
PstI C_TGCA'G
Cuts at: 830 3791 830
Size: 2961 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6439
PvuII CAG'CTG
Cuts at: 2861 2861
Size: 6439
SacI G_AGCT'C
Cuts at: 2325 3008 3428 2325
Size: 683 420 5336
Fragments arranged by size:
5336 683 420
SalI G'TCGA_C
Cuts at: 1936 3781 1936
Size: 1845 4594
SbfI CC_TGCA'GG
Cuts at: 3791 3791
Size: 6439
ScaI AGT'ACT
Cuts at: 4687 6399 4687
Size: 1712 4727
SfcI C'TryA_G
Cuts at: 826 1045 3787 4922 5603 5794 826
Size: 219 2742 1135 681 191 1471
Fragments arranged by size:
2742 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6439
SmaI CCC'GGG
Cuts at: 1872 3766 1872
Size: 1894 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2894 4543 5444 5706 820
Size: 197 926 951 1649 901 262 1553
Fragments arranged by size:
1649 1553 951 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4230 493
Size: 3737 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6439
SphI G_CATG'C
Cuts at: 1132 3797 1132
Size: 2665 3774
SspI AAT'ATT
Cuts at: 5 52 4363 5
Size: 47 4311 2081
Fragments arranged by size:
4311 2081 47
StuI AGG'CCT
Cuts at: 1517 3012 3180 3516 1517
Size: 1495 168 336 4440
Fragments arranged by size:
4440 1495 336 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 3878 513
Size: 552 939 258 1616 3074
Fragments arranged by size:
3074 1616 939 552 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 4466 4483 4636 4821 5266 325
Size: 1255 1188 1698 17 153 185 445 1498
Fragments arranged by size:
1698 1498 1255 1188 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2467 4685 6397
Size: 274 80 33 51 1443 490 2218 1712
Cuts at: 6397 96
Size: 138
Fragments arranged by size:
2218 1712 1443 490 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2352 2384 6399 6431 2352
Size: 32 4015 32 2360
Fragments arranged by size:
4015 2360 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6439
XbaI T'CTAG_A
Cuts at: 3775 3775
Size: 6439
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6439
XmaI C'CCGG_G
Cuts at: 1870 3764 1870
Size: 1894 4545
XmnI GAAnn'nnTTC
Cuts at: 2026 3245 3329 3413 3581 4568 2026
Size: 1219 84 84 168 987 3897
Fragments arranged by size:
3897 1219 987 168 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4247 276
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI