Restriction map for
pFN21AB7878
(Circular) (Six-base) MAPSORT of: pFN21AB7878.seq Check: 4032 from: 1 to: 6622
pFN21AB7878 6622 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2804 2804
Size: 6622
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3811 3965 1937
Size: 1874 154 4594
Fragments arranged by size:
4594 1874 154
Acc65I G'GTAC_C
Cuts at: 3943 3943
Size: 6622
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3087 3275 4685 6069 6337 1632
Size: 1455 188 1410 1384 268 1917
Fragments arranged by size:
1917 1455 1410 1384 268 188
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 2658 5521 6498 1203
Size: 1455 2863 977 1327
Fragments arranged by size:
2863 1455 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 2446 5351 2446
Size: 2905 3717
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 3821 4365 5937 3821
Size: 544 1572 4506
Fragments arranged by size:
4506 1572 544
ApaLI G'TGCA_C
Cuts at: 4679 5835 4679
Size: 1156 5466
ApoI r'AATT_y
Cuts at: 1493 3836 3931 4157 4309 6418 1493
Size: 2343 95 226 152 2109 1697
Fragments arranged by size:
2343 2109 1697 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 3947 1870
Size: 73 2004 4545
Fragments arranged by size:
4545 2004 73
BamHI G'GATC_C
Cuts at: 2032 2116 3952 4319 2032
Size: 84 1836 367 4335
Fragments arranged by size:
4335 1836 367 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3943 5398 618
Size: 325 217 627 2156 1455 1842
Fragments arranged by size:
2156 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3941 1391
Size: 2550 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 2657 3255 3468 3866 928
Size: 971 399 359 598 213 398 3684
Fragments arranged by size:
3684 971 598 399 398 359 213
BbvCI CC'TCA_GC
Cuts at: 2010 2583 2010
Size: 573 6049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2094 2128 4813 4847 1903
Size: 34 50 34 73 34 2685 34 3678
Fragments arranged by size:
3678 2685 73 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 3897 4514 5724 2272
Size: 1625 617 1210 3170
Fragments arranged by size:
3170 1625 1210 617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5233 136
Size: 107 122 71 631 605 3561 1525
Fragments arranged by size:
3561 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2095 6617 2095
Size: 4522 2100
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 4683 5839 1790
Size: 2893 1156 2573
Fragments arranged by size:
2893 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 2729 3631
Size: 416 53 83 399 24 858 454 902
Cuts at: 3631 4353 5311 442
Size: 722 958 1753
Fragments arranged by size:
1753 958 902 858 722 454 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 3476 3528 3903 5282 1239
Size: 998 1239 52 375 1379 2579
Fragments arranged by size:
2579 1379 1239 998 375 52
Bpu10I CC'TnA_GC
Cuts at: 2010 2583 2010
Size: 573 6049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4747 5612 5910 4747
Size: 865 298 5459
Fragments arranged by size:
5459 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3349 5285 882
Size: 617 1850 1936 2219
Fragments arranged by size:
2219 1936 1850 617
BsaAI yAC'GTr
Cuts at: 493 1204 4413 493
Size: 711 3209 2702
Fragments arranged by size:
3209 2702 711
BsaBI GATnn'nnATC
Cuts at: 6616 6616
Size: 6622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4429 4811 5446 275
Size: 53 83 186 936 2896 382 635 1451
Fragments arranged by size:
2896 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5055 5453 5727 5874 6285 5055
Size: 398 274 147 411 5392
Fragments arranged by size:
5392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3876 3906 1178
Size: 30 165 30 2473 30 3894
Fragments arranged by size:
3894 2473 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3092 3101 3167 3170 3479 1186
Size: 1906 9 66 3 309 4329
Fragments arranged by size:
4329 1906 309 66 9 3
BseYI C'CCAG_C
Cuts at: 3821 5825 6321 3821
Size: 2004 496 4122
Fragments arranged by size:
4122 2004 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2829 1163
Size: 573 1093 4956
Fragments arranged by size:
4956 1093 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4072 4833 5861 1540
Size: 275 186 2071 761 1028 2301
Fragments arranged by size:
2301 2071 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2093 3009 4683 4768 5839 2093
Size: 916 1674 85 1071 2876
Fragments arranged by size:
2876 1674 1071 916 85
BsmI GAATG_Cn'
Cuts at: 4133 4226 4133
Size: 93 6529
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 3009 3941 4683 4768 5839 1391
Size: 399 303 916 932 742 85 1071 2174
Fragments arranged by size:
2174 1071 932 916 742 399 303 85
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 4511 6241 4511
Size: 1730 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 3977 844
Size: 1960 1173 3489
Fragments arranged by size:
3489 1960 1173
BsrBI CCG'CTC
Cuts at: 1394 1823 4509 1394
Size: 429 2686 3507
Fragments arranged by size:
3507 2686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3508 5117 5291 65
Size: 1183 2260 1609 174 1396
Fragments arranged by size:
2260 1609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2748 5266 5453 1700
Size: 110 938 2518 187 2869
Fragments arranged by size:
2869 2518 938 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 4682 5694 4682
Size: 1012 5610
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6622
BstBI TT'CG_AA
Cuts at: 2056 5426 2056
Size: 3370 3252
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6622
BstXI CCAn_nnnn'nTGG
Cuts at: 3366 3366
Size: 6622
BstYI r'GATC_y
Cuts at: 1972 2032 2095 2116 3952 4319 4704 4721 6173
Size: 60 63 21 1836 367 385 17 1452
Cuts at: 6173 6259 6271 6617 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1836 1452 385 367 346 86 63
60 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6070
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5883
Fragments arranged by size:
5883 683 56
BtsI GCAGTG_nn'
Cuts at: 2817 3133 4138 4932 4952 2817
Size: 316 1005 794 20 4487
Fragments arranged by size:
4487 1005 794 316 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2328 2363 529
Size: 35 1764 35 4788
Fragments arranged by size:
4788 1764 35 35
DraI TTT'AAA
Cuts at: 2881 3926 2881
Size: 1045 5577
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5629 809
Size: 507 4313 1802
Fragments arranged by size:
4313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587
Size: 54 1425 50 275 18 2239 889 1629
Cuts at: 6587 8
Size: 43
Fragments arranged by size:
2239 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 2518 2853 2997 3159 3165 3278 4552
Size: 1035 130 335 144 162 6 113 1274
Cuts at: 4552 1353
Size: 3423
Fragments arranged by size:
3423 1274 1035 335 162 144 130 113
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2025 5209 5583 5729 387
Size: 1064 138 436 3184 374 146 1280
Fragments arranged by size:
3184 1280 1064 436 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 3087 3275 3476 3528 3903 4685
Size: 393 605 850 188 201 52 375 782
Cuts at: 4685 5282 6069 6337 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 850 787 782 605 597 393 375
268 201 188 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2850 2915 3257 2850
Size: 65 342 6215
Fragments arranged by size:
6215 342 65
EcoO109I rG'GnC_Cy
Cuts at: 2587 2924 3667 3761 2587
Size: 337 743 94 5448
Fragments arranged by size:
5448 743 337 94
EcoRI G'AATT_C
Cuts at: 3931 4309 3931
Size: 378 6244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 6622
FspI TGC'GCA
Cuts at: 5128 5128
Size: 6622
HaeII r_GCGC'y
Cuts at: 1678 5769 1678
Size: 4091 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2080 2112 2432 2464 2830
Size: 32 135 32 508 32 320 32 366
Cuts at: 2830 2862 2941 2973 3200 3232 3460 3492 5263
Size: 32 79 32 227 32 228 32 1771
Cuts at: 5263 5295 5337 5369 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1771 508 366 320 228 227 135
79 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3844 3966 4212 669
Size: 988 281 1906 122 246 3079
Fragments arranged by size:
3079 1906 988 281 246 122
HindIII A'AGCT_T
Cuts at: 748 3779 748
Size: 3031 3591
HpaI GTT'AAC
Cuts at: 3844 4212 3844
Size: 368 6254
KpnI G_GTAC'C
Cuts at: 3947 3947
Size: 6622
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2438 3054 5736 5920 1305
Size: 1133 616 2682 184 2007
Fragments arranged by size:
2682 2007 1133 616 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4800
Fragments arranged by size:
4800 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2775 3364 4580 4939 5098 518
Size: 648 1609 589 1216 359 159 2042
Fragments arranged by size:
2042 1609 1216 648 589 359 159
MspA1I CmG'CkG
Cuts at: 1606 2322 2549 4038 4715 5863 6108 1606
Size: 716 227 1489 677 1148 245 2120
Fragments arranged by size:
2120 1489 1148 716 677 245 227
NaeI GCC'GGC
Cuts at: 2750 2750
Size: 6622
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6070
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NgoMIV G'CCGG_C
Cuts at: 2748 2748
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 2662 3980 5525 1132
Size: 97 1433 1318 1545 2229
Fragments arranged by size:
2229 1545 1433 1318 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6622
PciI A'CATG_T
Cuts at: 2658 5521 2658
Size: 2863 3759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3821 1108
Size: 2713 3909
PfoI T'CCnGG_A
Cuts at: 2235 2235
Size: 6622
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2924 3761 2587
Size: 337 837 5448
Fragments arranged by size:
5448 837 337
PshAI GACnn'nnGTC
Cuts at: 1613 3545 3577 1613
Size: 1932 32 4658
Fragments arranged by size:
4658 1932 32
PsiI TTA'TAA
Cuts at: 2402 4192 2402
Size: 1790 4832
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 6590
PstI C_TGCA'G
Cuts at: 830 2580 3868 3974 830
Size: 1750 1288 106 3478
Fragments arranged by size:
3478 1750 1288 106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
PvuII CAG'CTG
Cuts at: 2322 2549 2322
Size: 227 6395
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SapI GCTCTTCn'nnn_
Cuts at: 2997 2997
Size: 6622
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SfcI C'TryA_G
Cuts at: 826 1045 2576 3243 3864 3970 5105 5786 5977
Size: 219 1531 667 621 106 1135 681 191
Cuts at: 5977 826
Size: 1471
Fragments arranged by size:
1531 1471 1135 681 667 621 219 191
106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3949 1872
Size: 2077 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4726 5627 5889 820
Size: 197 926 2783 901 262 1553
Fragments arranged by size:
2783 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3980 1132
Size: 2848 3774
SspI AAT'ATT
Cuts at: 5 52 4546 5
Size: 47 4494 2081
Fragments arranged by size:
4494 2081 47
StuI AGG'CCT
Cuts at: 1517 3888 1517
Size: 2371 4251
StyI C'CwwG_G
Cuts at: 513 1065 3575 4061 513
Size: 552 2510 486 3074
Fragments arranged by size:
3074 2510 552 486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3029 3797 4649 4666 4819 5004 5449
Size: 1255 1449 768 852 17 153 185 445
Cuts at: 5449 325
Size: 1498
Fragments arranged by size:
1498 1449 1255 852 768 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2969 4868 6580
Size: 274 80 33 51 1443 992 1899 1712
Cuts at: 6580 96
Size: 138
Fragments arranged by size:
1899 1712 1443 992 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6582 6614 6582
Size: 32 6590
Tth111I GACn'n_nGTC
Cuts at: 1316 2603 1316
Size: 1287 5335
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2924 2924
Size: 6622
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6622
XmaI C'CCGG_G
Cuts at: 1870 3947 1870
Size: 2077 4545
XmnI GAAnn'nnTTC
Cuts at: 4751 4751
Size: 6622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI