Restriction map for
pFN21AB7876
(Circular) (Six-base) MAPSORT of: pFN21AB7876.seq Check: 1087 from: 1 to: 6619
pFN21AB7876 6619 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3962 1937
Size: 2025 4594
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2201 2755 3175 3511 4682 6066 6334
Size: 370 199 554 420 336 1171 1384 268
Cuts at: 6334 1632
Size: 1917
Fragments arranged by size:
1917 1384 1171 554 420 370 336 268
199
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 5518 6495 1203
Size: 4315 977 1327
Fragments arranged by size:
4315 1327 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6619
AlwNI CAG_nnn'CTG
Cuts at: 4362 5934 4362
Size: 1572 5047
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 2151 2501 3549 3928 4154 4306 6415 1493
Size: 658 350 1048 379 226 152 2109 1697
Fragments arranged by size:
2109 1697 1048 658 379 350 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 3944 1870
Size: 73 2001 4545
Fragments arranged by size:
4545 2001 73
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3940 5395 618
Size: 325 217 627 2153 1455 1842
Fragments arranged by size:
2153 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2389 3458 3901 3938 1391
Size: 998 1069 443 37 4072
Fragments arranged by size:
4072 1069 998 443 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5648
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4810 4844 1903
Size: 34 50 34 2789 34 3678
Fragments arranged by size:
3678 2789 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BfrBI ATG'CAT
Cuts at: 3251 3637 3721 3251
Size: 386 84 6149
Fragments arranged by size:
6149 386 84
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5230 136
Size: 107 122 71 631 605 3558 1525
Fragments arranged by size:
3558 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3194 3530 6614 3194
Size: 336 3084 3199
Fragments arranged by size:
3199 3084 336
BlpI GC'TnA_GC
Cuts at: 4036 4036
Size: 6619
Bme1580I G_kGCm'C
Cuts at: 1790 4680 5836 1790
Size: 2890 1156 2573
Fragments arranged by size:
2890 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6619
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2655 2739 2859
Size: 416 53 83 399 24 1238 84 120
Cuts at: 2859 3027 3363 3911 4350 5308 442
Size: 168 336 548 439 958 1753
Fragments arranged by size:
1753 1238 958 548 439 416 399 336
168 120 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2132 2132
Size: 6619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 2583 3003 3087 3171 3423 3507 3591
Size: 978 366 420 84 84 252 84 84
Cuts at: 3591 3675 3759 3843 5279 1239
Size: 84 84 84 1436 2579
Fragments arranged by size:
2579 1436 978 420 366 252 84 84
84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4744 5609 5907 4744
Size: 865 298 5456
Fragments arranged by size:
5456 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5282 882
Size: 617 3783 2219
Fragments arranged by size:
3783 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4410 493
Size: 711 3206 2702
Fragments arranged by size:
3206 2702 711
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4426 4808 5443 275
Size: 53 83 186 936 2893 382 635 1451
Fragments arranged by size:
2893 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5052 5450 5724 5871 6282 5052
Size: 398 274 147 411 5389
Fragments arranged by size:
5389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 1178
Size: 30 165 30 942 30 5422
Fragments arranged by size:
5422 942 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2353 1186
Size: 1167 5452
BseYI C'CCAG_C
Cuts at: 5822 6318 5822
Size: 496 6123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6046
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4069 4830 5858 1540
Size: 275 186 2068 761 1028 2301
Fragments arranged by size:
2301 2068 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2245 3458 4680 4765 5836 2245
Size: 1213 1222 85 1071 3028
Fragments arranged by size:
3028 1222 1213 1071 85
BsmI GAATG_Cn'
Cuts at: 2426 2684 2852 2979 3425 3509 4130 4223 2426
Size: 258 168 127 446 84 621 93 4822
Fragments arranged by size:
4822 621 446 258 168 127 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2245 2389 3458 3901 3938 4680 4765
Size: 399 455 144 1069 443 37 742 85
Cuts at: 4765 5836 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1069 742 455 443 399 144
85 37
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 3133 4508 6238 3133
Size: 1375 1730 3514
Fragments arranged by size:
3514 1730 1375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3974 844
Size: 3130 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4506 1394
Size: 429 2683 3507
Fragments arranged by size:
3507 2683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3182 3518 3770 5114 5288 65
Size: 1183 1934 336 252 1344 174 1396
Fragments arranged by size:
1934 1396 1344 1183 336 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5263 5450 1700
Size: 110 3453 187 2869
Fragments arranged by size:
3453 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6619
BstBI TT'CG_AA
Cuts at: 2623 3799 5423 2623
Size: 1176 1624 3819
Fragments arranged by size:
3819 1624 1176
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstYI r'GATC_y
Cuts at: 1972 3194 3437 3530 3614 3949 4316 4701 4718
Size: 1222 243 93 84 335 367 385 17
Cuts at: 4718 6170 6256 6268 6614 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1222 385 367 346 335 243
93 86 84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2476 513
Size: 552 1411 4656
Fragments arranged by size:
4656 1411 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 2224 2245 3434 3602 3686 3901 4135 4929 4949
Size: 21 1189 168 84 215 234 794 20
Cuts at: 4949 2224
Size: 3894
Fragments arranged by size:
3894 1189 794 234 215 168 84 21
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3076 3111 3412 3447 3496 3531 529
Size: 35 2512 35 301 35 49 35 3617
Fragments arranged by size:
3617 2512 301 49 35 35 35 35
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4066 4955 6584
Size: 54 1425 50 275 18 2236 889 1629
Cuts at: 6584 8
Size: 43
Fragments arranged by size:
2236 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2782 3315 4549 1353
Size: 1429 533 1234 3423
Fragments arranged by size:
3423 1429 1234 533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3456 3456
Size: 6619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2201 2217 2583 2755 3003 3087
Size: 393 370 199 16 366 172 248 84
Cuts at: 3087 3171 3175 3423 3507 3511 3591 3675 3759
Size: 84 4 248 84 4 80 84 84
Cuts at: 3759 3843 4682 5279 6066 6334 1239
Size: 84 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 597 393 370 366 268
248 248 199 172 84 84 84 84
84 84 80 16 4 4
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
EcoRV GAT'ATC
Cuts at: 2991 3131 3467 2991
Size: 140 336 6143
Fragments arranged by size:
6143 336 140
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 5766 1678
Size: 4088 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5260 5292 5334 5366 1373
Size: 32 135 32 3688 32 42 32 2626
Fragments arranged by size:
3688 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3963 4209 669
Size: 988 281 2025 246 3079
Fragments arranged by size:
3079 2025 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3774 748
Size: 3026 3593
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5733 5917 1305
Size: 4428 184 2007
Fragments arranged by size:
4428 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4797
Fragments arranged by size:
4797 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 2326 2578 2746 2830 2857 3384
Size: 648 976 184 252 168 84 27 527
Cuts at: 3384 4577 4936 5095 518
Size: 1193 359 159 2042
Fragments arranged by size:
2042 1193 976 648 527 359 252 184
168 159 84 27
MspA1I CmG'CkG
Cuts at: 1606 4035 4712 5860 6105 1606
Size: 2429 677 1148 245 2120
Fragments arranged by size:
2429 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6067
NdeI CA'TA_TG
Cuts at: 387 2293 2743 2827 387
Size: 1906 450 84 4179
Fragments arranged by size:
4179 1906 450 84
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NruI TCG'CGA
Cuts at: 3709 3709
Size: 6619
NsiI A_TGCA'T
Cuts at: 3253 3639 3723 3253
Size: 386 84 6149
Fragments arranged by size:
6149 386 84
NspI r_CATG'y
Cuts at: 1132 1229 2179 2703 3255 3977 5522 1132
Size: 97 950 524 552 722 1545 2229
Fragments arranged by size:
2229 1545 950 722 552 524 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6619
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2015 2047 4688 4720 6201 6233 2015
Size: 32 2641 32 1481 32 2401
Fragments arranged by size:
2641 2401 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 2493 2661 4189 2493
Size: 168 1528 4923
Fragments arranged by size:
4923 1528 168
PstI C_TGCA'G
Cuts at: 830 3971 830
Size: 3141 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
SacI G_AGCT'C
Cuts at: 3458 3458
Size: 6619
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SapI GCTCTTCn'nnn_
Cuts at: 2782 2782
Size: 6619
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 4867 6579 4867
Size: 1712 4907
SfcI C'TryA_G
Cuts at: 826 1045 2034 3967 5102 5783 5974 826
Size: 219 989 1933 1135 681 191 1471
Fragments arranged by size:
1933 1471 1135 989 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 3946 1872
Size: 2074 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4723 5624 5886 820
Size: 197 926 2780 901 262 1553
Fragments arranged by size:
2780 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 4543 5
Size: 47 4491 2081
Fragments arranged by size:
4491 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6619
StyI C'CwwG_G
Cuts at: 513 1065 4058 513
Size: 552 2993 3074
Fragments arranged by size:
3074 2993 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4646 4663 4816 5001 5446 325
Size: 1255 3066 17 153 185 445 1498
Fragments arranged by size:
3066 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4865 6577 96
Size: 274 80 33 51 1443 2888 1712 138
Fragments arranged by size:
2888 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6579 6611 6579
Size: 32 6587
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6619
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 3944 1870
Size: 2074 4545
XmnI GAAnn'nnTTC
Cuts at: 2684 2768 3188 3356 4748 2684
Size: 84 420 168 1392 4555
Fragments arranged by size:
4555 1392 420 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI