Restriction map for
pFN21AB7875
(Circular) (Six-base) MAPSORT of: pFN21AB7875.seq Check: 2850 from: 1 to: 6415
pFN21AB7875 6415 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 4478 5862 6130 1632
Size: 404 2442 1384 268 1917
Fragments arranged by size:
2442 1917 1384 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 5314 6291 1203
Size: 4111 977 1327
Fragments arranged by size:
4111 1327 977
AgeI A'CCGG_T
Cuts at: 2901 2985 3405 5246 2901
Size: 84 420 1841 4070
Fragments arranged by size:
4070 1841 420 84
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2068 1184
Size: 884 5531
AlwNI CAG_nnn'CTG
Cuts at: 4158 5730 4158
Size: 1572 4843
ApaLI G'TGCA_C
Cuts at: 4472 5628 4472
Size: 1156 5259
ApoI r'AATT_y
Cuts at: 1493 2132 2567 2679 2695 2763 2931 3200 3267
Size: 639 435 112 16 68 168 269 67
Cuts at: 3267 3724 3950 4102 6211 1493
Size: 457 226 152 2109 1697
Fragments arranged by size:
2109 1697 639 457 435 269 226 168
152 112 68 67 16
AseI AT'TA_AT
Cuts at: 160 1032 4969 160
Size: 872 3937 1606
Fragments arranged by size:
3937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 3740 1870
Size: 73 1797 4545
Fragments arranged by size:
4545 1797 73
BamHI G'GATC_C
Cuts at: 3745 4112 3745
Size: 367 6048
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3736 5191 618
Size: 325 217 627 1949 1455 1842
Fragments arranged by size:
1949 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2286 3734 1391
Size: 895 1448 4072
Fragments arranged by size:
4072 1448 895
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2212 3570 928
Size: 971 313 1358 3773
Fragments arranged by size:
3773 1358 971 313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2040 4307 5517 2040
Size: 2267 1210 2938
Fragments arranged by size:
2938 2267 1210
BclI T'GATC_A
Cuts at: 1548 2256 1548
Size: 708 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5026 136
Size: 107 122 71 631 605 3354 1525
Fragments arranged by size:
3354 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 2169 3832 2169
Size: 1663 4752
Bme1580I G_kGCm'C
Cuts at: 1790 4476 5632 1790
Size: 2686 1156 2573
Fragments arranged by size:
2686 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2318 4146 5104
Size: 416 53 83 399 24 901 1828 958
Cuts at: 5104 442
Size: 1753
Fragments arranged by size:
1828 1753 958 901 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2162 2793 2877 2961 3045 3129 3213
Size: 804 119 631 84 84 84 84 84
Cuts at: 3213 3297 3381 3465 3633 3715 5075 1239
Size: 84 84 84 168 82 1360 2579
Fragments arranged by size:
2579 1360 804 631 168 119 84 84
84 84 84 84 84 84 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 4540 5405 5703 2021
Size: 2519 865 298 2733
Fragments arranged by size:
2733 2519 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5078 882
Size: 617 3579 2219
Fragments arranged by size:
3579 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4206 493
Size: 711 3002 2702
Fragments arranged by size:
3002 2702 711
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4222 4604 5239 275
Size: 53 83 186 936 2689 382 635 1451
Fragments arranged by size:
2689 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2901 2985 3405 4848 5246 5520 5667 6078 2901
Size: 84 420 1443 398 274 147 411 3238
Fragments arranged by size:
3238 1443 420 411 398 274 147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6190
Fragments arranged by size:
6190 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 1186
Size: 973 5442
BseYI C'CCAG_C
Cuts at: 5618 6114 5618
Size: 496 5919
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 3324 3408 1163
Size: 573 723 865 84 4170
Fragments arranged by size:
4170 865 723 573 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 4626 5654 1540
Size: 275 186 1864 761 1028 2301
Fragments arranged by size:
2301 1864 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4476 4561 5632 4476
Size: 85 1071 5259
Fragments arranged by size:
5259 1071 85
BsmI GAATG_Cn'
Cuts at: 3926 4019 3926
Size: 93 6322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 3734 4476 4561 5632 1391
Size: 399 496 1448 742 85 1071 2174
Fragments arranged by size:
2174 1448 1071 742 496 399 85
BspEI T'CCGG_A
Cuts at: 6078 6078
Size: 6415
BspHI T'CATG_A
Cuts at: 4304 6034 4304
Size: 1730 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2219 3770 844
Size: 1375 1551 3489
Fragments arranged by size:
3489 1551 1375
BsrBI CCG'CTC
Cuts at: 1394 1823 4302 1394
Size: 429 2479 3507
Fragments arranged by size:
3507 2479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2159 4910 5084 65
Size: 1183 911 2751 174 1396
Fragments arranged by size:
2751 1396 1183 911 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2901 2985 3405 5059 5246 1700
Size: 110 1091 84 420 1654 187 2869
Fragments arranged by size:
2869 1654 1091 420 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 4475 5487 4475
Size: 1012 5403
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2234 794
Size: 1440 4975
BstBI TT'CG_AA
Cuts at: 5219 5219
Size: 6415
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 3745 4112 4497 4514 5966 6052 6064 6410
Size: 1773 367 385 17 1452 86 12 346
Cuts at: 6410 1972
Size: 1977
Fragments arranged by size:
1977 1773 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4924
Fragments arranged by size:
4924 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2321 487
Size: 683 56 1095 4581
Fragments arranged by size:
4581 1095 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 3931 4725 4745 2163
Size: 1768 794 20 3833
Fragments arranged by size:
3833 1768 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6380
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5422 809
Size: 507 4106 1802
Fragments arranged by size:
4106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2357 3862
Size: 54 1425 50 275 18 294 233 1505
Cuts at: 3862 4751 6380 8
Size: 889 1629 43
Fragments arranged by size:
1629 1505 1425 889 294 275 233 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 4345 1353
Size: 938 2054 3423
Fragments arranged by size:
3423 2054 938
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5002 5376 5522 387
Size: 1064 138 3413 374 146 1280
Fragments arranged by size:
3413 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2043 2162 2793 2877 2961 3045
Size: 393 404 7 119 631 84 84 84
Cuts at: 3045 3129 3213 3297 3381 3465 3633 3715 4478
Size: 84 84 84 84 84 168 82 763
Cuts at: 4478 5075 5862 6130 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 763 631 597 404 393 268
168 119 84 84 84 84 84 84
84 84 82 7
EcoRI G'AATT_C
Cuts at: 2679 2763 2931 3267 3724 4102 2679
Size: 84 168 336 457 378 4992
Fragments arranged by size:
4992 457 378 336 168 84
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 5562 1678
Size: 3884 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 2299 2331 5056
Size: 32 135 32 676 32 19 32 2725
Cuts at: 5056 5088 5130 5162 1373
Size: 32 42 32 2626
Fragments arranged by size:
2725 2626 676 135 42 32 32 32
32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 4005 669
Size: 988 281 1821 246 3079
Fragments arranged by size:
3079 1821 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2808 3060 3144 3312 3480 3648 748
Size: 2060 252 84 168 168 168 3515
Fragments arranged by size:
3515 2060 252 168 168 168 84
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5529 5713 1305
Size: 4224 184 2007
Fragments arranged by size:
4224 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 2359 10
Size: 54 1425 343 294 233 4066
Fragments arranged by size:
4066 1425 343 294 233 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4373 4732 4891 518
Size: 648 3207 359 159 2042
Fragments arranged by size:
3207 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3831 4508 5656 5901 1606
Size: 2225 677 1148 245 2120
Fragments arranged by size:
2225 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4924
Fragments arranged by size:
4924 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 2306 2653 3773 5318 1132
Size: 97 1077 347 1120 1545 2229
Fragments arranged by size:
2229 1545 1120 1077 347 97
PasI CC'CwG_GG
Cuts at: 1348 2057 1348
Size: 709 5706
PciI A'CATG_T
Cuts at: 5314 5314
Size: 6415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6415
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 5997 6029 4484
Size: 32 1481 32 4870
Fragments arranged by size:
4870 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 2565 3985 2565
Size: 1420 4995
PstI C_TGCA'G
Cuts at: 830 3767 830
Size: 2937 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SbfI CC_TGCA'GG
Cuts at: 3767 3767
Size: 6415
ScaI AGT'ACT
Cuts at: 4663 6375 4663
Size: 1712 4703
SexAI A'CCwGG_T
Cuts at: 2214 2214
Size: 6415
SfcI C'TryA_G
Cuts at: 826 1045 2206 2869 2953 3763 4898 5579 5770
Size: 219 1161 663 84 810 1135 681 191
Cuts at: 5770 826
Size: 1471
Fragments arranged by size:
1471 1161 1135 810 681 663 219 191
84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 4519 5420 5682 820
Size: 197 926 93 2483 901 262 1553
Fragments arranged by size:
2483 1553 926 901 262 197 93
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 2653 3773 1132
Size: 1521 1120 3774
Fragments arranged by size:
3774 1521 1120
SspI AAT'ATT
Cuts at: 5 52 2532 4339 5
Size: 47 2480 1807 2081
Fragments arranged by size:
2480 2081 1807 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 2004 3854 513
Size: 552 939 1850 3074
Fragments arranged by size:
3074 1850 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4442 4459 4612 4797 5242 325
Size: 1255 2862 17 153 185 445 1498
Fragments arranged by size:
2862 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 4661 6373
Size: 274 80 33 51 1443 105 2579 1712
Cuts at: 6373 96
Size: 138
Fragments arranged by size:
2579 1712 1443 274 138 105 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6375 6407 6375
Size: 32 6383
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6415
XbaI T'CTAG_A
Cuts at: 2266 3751 2266
Size: 1485 4930
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6415
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 2532 2615 4544 2532
Size: 83 1929 4403
Fragments arranged by size:
4403 1929 83
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI